| dna probe for the identification of histoplasma capsulatum. | a 1.85-kilobase hindiii nuclear dna probe from histoplasma capsulatum g217b detected polymorphic restriction fragments within whole-cell dna from different clinical isolates of h. capsulatum, consistent with the previous system of classification. the probe failed to hybridize to dna from blastomyces dermatitidis, candida spp., saccharomyces cerevisiae, sepedonium chrysospermum, and chrysosporium keratinophilum under low-stringency conditions and therefore may have value as a diagnostic reagent t ... | 1989 | 2573616 |
| efficacy of interferon-gamma and amphotericin b for the treatment of systemic murine histoplasmosis. | the number of cases of systemic histoplasmosis has increased substantially in recent years, and improved therapy is needed. we examined the efficacy of immunomodulation with interferon (ifn)-gamma alone or in combination with a suboptimal regimen of amphotericin b for the treatment of primary systemic murine histoplasmosis. in the first study, balb/c mice were infected with histoplasma capsulatum g217b and treated with 10(5) u of ifn given every other day either preinfection and postinfection or ... | 2001 | 11226849 |
| utilization and cell-surface binding of hemin by histoplasma capsulatum. | histoplasma capsulatum, a dimorphic fungus capable of causing severe respiratory illness in immunocompromised individuals, resides in macrophages during mammalian infection. previous studies suggest that siderophore-mediated iron transport may be important for the acquisition of iron from transferrin while the organism resides in macrophages. however, iron is also present as hemin in the intracellular environment of the macrophage and may serve as a major source of iron during infection. thus th ... | 2002 | 12109883 |
| comparative serological evaluation of 10 blastomyces dermatitidis yeast phase lysate antigens from different sources. | yeast phase lysate antigens from 10 isolates of blastomyces dermatitidis (dog, erc-2 and t-58; t-27, polar bear; woodpile, er-3; bat lung, 48938; human, b5929, b5895, b5896, b5931, and capp) from different geographical regions, in addition to a histoplasma capsulatum (g217b) lysate preparation were compared with respect to their reactivity against serum specimens from dogs, rabbits and humans positive for blastomycosis using an indirect enzyme-linked immunosorbent assay. in addition, the lysate ... | 2004 | 15078431 |
| fosmid-based physical mapping of the histoplasma capsulatum genome. | a fosmid library representing 10-fold coverage of the histoplasma capsulatum g217b genome was used to construct a restriction-based physical map. the data obtained from three restriction endonuclease fingerprints, generated from each clone using bamhi, hindiii, and psti endonucleases, were combined and used in fpc for automatic and manual contig assembly builds. concomitantly, a whole-genome shotgun (wgs) sequencing of paired-end reads from plasmids and fosmids were assembled with pcap, providin ... | 2004 | 15289478 |