| comparison and differentiation of potyvirus isolates and identification of strain-, virus-, subgroup-specific and potyvirus group-common epitopes using monoclonal antibodies. | a panel of monoclonal antibodies (mabs) generated against an admixture of 12 potyvirus isolates was used to compare and differentiate diverse potyviruses. both native and denatured virions of strains of bean yellow mosaic (bymv), potato virus y, tobacco etch, pea seed-borne mosaic, iris severe mosaic, iris mild mosaic and asparagus virus-1 potyviruses were used as immunogen and as antigen for screening of the hybridoma cell lines. thirty cell lines secreting potyvirus-specific antibodies reactiv ... | 1991 | 1703561 |
| properties of a virus causing mosaic and leaf curl disease of celosia argentea l. in nigeria. | a sap transmissible virus, causing mosaic and leaf curl disease of celosia argentea, was isolated at vegetable farms in amuwo odofin, tejuoso, and abule ado, lagos, nigeria. the virus had a restricted host range confined to a few species of the amaranthaceae, chenopodiaceae and solanaceae families. it failed to infect several other species of the aizoaceae, brassicaceae, cucurbitaceae, fabaceae, lamiaceae, malvaceae, poaceae and tiliaceae families. the virus was transmitted in a non-persistent m ... | 1998 | 9842442 |
| differentiation of celosia mosaic virus and asparagus virus 1 based on biological properties. | an attempt was made to distinguish between celosia mosaic virus (cimv) and asparagus virus 1 (av-1) based on biological properties, which hitherto was obscured from serological data from previous work. the host range of av-1 was found to be a subset of that of cimv and av-1 was transmitted by the aphid myzus persicae which, on the other hand, did not transmit cimv. no evidence of cross-protection was obtained between these two viruses. | 2000 | 11155362 |
| detection of plant viruses in mixed infection by a macroarray-assisted method. | the protocol for a simple, sensitive, and specific method using a cdna macroarray to detect multiple viruses is provided. the method can be used even at the production sites for crops, which need a reliable routine diagnosis for mixed infection of plant viruses. the method consists of three steps: rna extraction, duplex rt-pcr, and "microtube hybridization" (mth). biotinylated cdna probes are prepared using rt-pcr and used to hybridize a nylon membrane containing target viral cdnas by mth. posit ... | 2015 | 25287491 |
| viruses of asparagus. | the current knowledge on viruses infecting asparagus (asparagus officinalis) is reviewed. over half a century, nine virus species belonging to the genera ilarvirus, cucumovirus, nepovirus, tobamovirus, potexvirus, and potyvirus have been found in this crop. the potyvirus asparagus virus 1 (av1) and the ilarvirus asparagus virus 2 (av2) are widespread and negatively affect the economic life of asparagus crops reducing yield and increasing the susceptibility to biotic and abiotic stress. the main ... | 2012 | 22682173 |
| complete genome sequences of two biologically distinct isolates of asparagus virus 1. | the complete genome sequences of two asparagus virus 1 (av-1) isolates differing in their ability to cause systemic infection in nicotiana benthamiana were determined. their genomes had 9,741 nucleotides excluding the 3'-terminal poly(a) tail, encoded a polyprotein of 3,112 amino acids, and shared 99.6 % nucleotide sequence identity. they differed at 37 nucleotide and 15 amino acid sequence positions (99.5 % identity) scattered over the polyprotein. the closest relatives of av-1 in amino acid se ... | 2015 | 25216774 |
| molecular identification and biological characterization of a new potyvirus in lettuce. | a potyvirus causing necrosis and leaf distortion on lettuce was found in the lazio region of italy. host range analysis showed its ability to infect only chenopodium quinoa and c. amaranticolor in addition to some lettuce cultivars. the virus could be transmitted by aphids of the species myzus persicae. the complete 9829-nt genome was characterized. blast analysis of sequence of the complete encoded polyprotein showed that the most closely related virus is asparagus virus 1, with 52 % amino acid ... | 2016 | 27287434 |