Publications

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in vivo replication kinetics and transcription patterns of the nucleopolyhedrovirus (neabnpv) of the balsam fir sawfly, neodiprion abietis.dna replication and transcription of neabnpv, the nucleopolyhedrovirus (npv) of the balsam fir sawfly, neodiprion abietis (hymenoptera: diprionidae), in host larvae were investigated. npv dna replication kinetics and gene-expression patterns have been resolved only in lepidopteran cell-culture systems and in limited in vivo experiments with lepidopteran larvae. furthermore, there are significant differences in pathologies caused by lepidopteran npvs, which replicate in many tissues, and hymenopt ...200717554027
sequence analysis and organization of the neodiprion abietis nucleopolyhedrovirus genome.of 30 baculovirus genomes that have been sequenced to date, the only nonlepidopteran baculoviruses include the dipteran culex nigripalpus nucleopolyhedrovirus and two hymenopteran nucleopolyhedroviruses that infect the sawflies neodiprion lecontei (nelenpv) and neodiprion sertifer (nesenpv). this study provides a complete sequence and genome analysis of the nucleopolyhedrovirus that infects the balsam fir sawfly neodiprion abietis (hymenoptera, symphyta, diprionidae). the n. abietis nucleopolyhe ...200616809301
influence of larval stage and virus inoculum on virus yield in insect host neodiprion abietis (hymenoptera: diprionidae).virus yield produced by dead larvae of balsam fir sawfly, neodiprion abietis (harris) (hymenoptera: diprionidae), that had been infected at four different larval stages (second, third, fourth, or fifth instar) with two virus concentrations (10(5) polyhedral inclusion bodies (pib) /ml or 10(7) pib/ml), were analyzed and compared to determine the effects of instar and amount of virus inoculum on virus production. the results indicate that both larval stage and inoculation dosage significantly affe ...200516539107
virulence of a nucleopolyhedrovirus to neodiprion abietis (hymenoptera: diprionidae).different larval stages of balsam fir sawfly, neodiprion abietis (harris) (hymenoptera: diprionidae), were challenged by different concentrations of a nucleopolyhedrovirus in the laboratory to determine larval susceptibility to the virus and to test treatment effects of the virus on the sawfly survivors. the results indicated that younger larvae were more susceptible to the virus than older instars. the speed of larval death depended on the larval age and the virus concentration. generally, the ...200516539106
advantages of a mixed diet: feeding on several foliar age classes increases the performance of a specialist insect herbivore.two field studies were carried out to determine the influence of abies balsamea foliage age on the preference and performance of larvae of neodiprion abietis, a specialist diprionid sawfly. preference was determined by examining n. abietis defoliation on all age classes of foliage. performance was estimated using larval survival, cocoon weights and the percentage of adults that were females. neodiprion abietis preference for, and performance on, current-year foliage was very low, peaked on 2 or ...200312721829
ecosystem alteration modifies the relative strengths of bottom-up and top-down forces in a herbivore population.1. ecosystem alterations can affect the abundance, distribution and diversity of plants and animals, and thus potentially change the relative strength of bottom-up (the plant resource) and top-down (natural enemies) trophic forces acting on herbivore populations. 2. the hypothesis that alterations of the forest ecosystem associated with precommercial thinning have contributed to the increased severity of outbreaks of neodiprion abietis (harris), a sawfly defoliator, through the reduction of trop ...200617009749
photoperiodism in the early balsam strain of the neodiprion abietis complex (hymenoptera: diprionidae). 19744832967
chromosome fission associated with growth of ribosomal dna in neodiprion abietis (hymenoptera: diprionidae).the haploid complement consists of seven metacentric chromosomes in most diprionid species but has evolved to n = 8 by fission in neodiprion abietis. this fission generated a small telocentric chromosome and a large pseudoacrocentric chromosome with a short arm carrying a satellite. in situ hybridization indicated that the location of the rrna gene cluster corresponds to the whole short arm. this suggests that (i) the breaking point was located close to an rrna gene cluster, and (ii) fission was ...200011052531
lethal and sublethal effects of a neem-based insecticide on balsam fir sawfly (hymenoptera: diprionidae).lethal and sublethal effects of neemix 4.5 ec, a commercial neem preparation, on balsam fir sawfly, neodiprion abietis (harris), were determined in the laboratory. larval mortality of n. abietis increased in a concentration-dependent manner, and lethal time decreased with increasing neemix 4.5 ec concentration. fifty percent of the larvae died after 4.6 d at a concentration of 90 ppm azadirachtin (aza) and 12.3 d at a concentration of 0.08 ppm. neemix 4.5 ec showed some deterrent effects to feed ...200312650342
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