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behavior of enterobacter pulveris in amorphous and crystalline powder matrices treated with supercritical carbon dioxide.the resistance of an enterobacter pulveris strain to combined heat and supercritical carbon dioxide (scco(2)) treatments in different powder matrices was examined. the strain proved resistant to scco(2) treatment up to 50 mpa pressure at temperatures >73 °c for at least 20 min in a commercial infant formula. water availability was shown to be important for the observed thermotolerance, because introduction of water in the scco(2) gas flow during treatment resulted in a 1 log(10) cfu/g reduction ...201222959935
draft genome sequences of three newly identified species in the genus cronobacter, c. helveticus lmg23732t, c. pulveris lmg24059, and c. zurichensis lmg23730t.cronobacter helveticus, cronobacter pulveris, and cronobacter zurichensis are newly described species in the cronobacter genus, which is associated with serious infections of neonates. this is the first report of draft genome sequences for these species.201324072872
genome sequences of two enterobacter pulveris strains, 601/05t (=lmg 24057t =dsm 19144t) and 1160/04 (=lmg 24058 =dsm 19146), isolated from fruit powder.we report the draft genome sequences of the enterobacter pulveris strains 601/05(t) (=lmg24057(t) =dsm19144(t)) and 1160/04 (=lmg24058 =dsm19146), isolated from fruit powder. the genome assemblies for the e. pulveris type strain, lmg24057, and strain lmg24058 have sizes of 4,708,624 and 4,811,103 bp and g+c contents of 56.6% and 56.5%, respectively.201324309737
taxonomic evaluation of the genus enterobacter based on multilocus sequence analysis (mlsa): proposal to reclassify e. nimipressuralis and e. amnigenus into lelliottia gen. nov. as lelliottia nimipressuralis comb. nov. and lelliottia amnigena comb. nov., respectively, e. gergoviae and e. pyrinus into pluralibacter gen. nov. as pluralibacter gergoviae comb. nov. and pluralibacter pyrinus comb. nov., respectively, e. cowanii, e. radicincitans, e. oryzae and e. arachidis into kosakonia gen. nov. as kosakonia cowanii comb. nov., kosakonia radicincitans comb. nov., kosakonia oryzae comb. nov. and kosakonia arachidis comb. nov., respectively, and e. turicensis, e. helveticus and e. pulveris into cronobacter as cronobacter zurichensis nom. nov., cronobacter helveticus comb. nov. and cronobacter pulveris comb. nov., respectively, and emended description of the genera enterobacter and cronobacter.the taxonomy of enterobacter has a complicated history, with several species transferred to and from this genus. classification of strains is difficult owing to its polyphyletic nature, based on 16s rrna gene sequences. it has been previously acknowledged that enterobacter contains species which should be transferred to other genera. in an attempt to resolve the taxonomy of enterobacter, mlsa based on partial sequencing of protein-encoding genes (gyrb, rpob, infb and atpd) was performed on the t ...201323632228
genotypic and phenotypic characteristics of cronobacter species, with particular attention to the newly reclassified species cronobacter helveticus, cronobacter pulveris, and cronobacter zurichensis.in 2013, enterobacter helveticus, enterobacter pulveris and enterobacter turicensis, were reclassified as cronobacter helveticus, cronobacter pulveris and cronobacter zurichensis, respectively. previously these species had been used as negative controls for some cronobacter detection assays. this study examined cultural, biochemical and molecular cronobacter detection and identification assays, with emphasis on the new species. additionally, 32 cronobacter genomes were examined for the presence ...201425084667
o antigen of franconibacterpulverisg3872 (o1) is a 4-deoxy-d-arabino-hexose-containing polysaccharide synthesized by the abc-transporter-dependent pathway.franconibacter (enterobacter, cronobacter) pulveris bacteria share several typical characteristics with, and hence pose a challenge for the detection of, cronobacter sakazakii, an emerging opportunistic pathogen, which can cause severe infections in neonates. a structurally variable o-specific polysaccharide (ops) called o antigen provides the major basis for the typing of gram-negative bacteria. we investigated the structure and genetics of the o antigen of f. pulveris g3872 (designated o1). an ...201627166227
plant growth promoting potential and phylogenetic characteristics of a lichenized nitrogen fixing bacterium, enterobacter cloacae.lichens are complex symbiotic association of mycobionts, photobionts, and bacteriobionts, including chemolithotropic bacteria. in the present study, 46 lichenized bacteria were isolated by conventional and enrichment culture methods on nitrogen-free bromothymol blue (nfb) medium. only 11 of the 46 isolates fixed nitrogen on nfb and had reduced acetylene. all these 11 isolates had also produced siderophore and 10 of them the iaa. further, ammonia production was recorded from nine of these nitroge ...201627273065
analysis of bacterial diversity during the fermentation of inyu, a high-temperature fermented soy sauce, using nested pcr-denaturing gradient gel electrophoresis and the plate count method.the diversity of bacteria associated with the fermentation of inyu, also known as black soy sauce, was studied through the nested pcr-denaturing gradient gel electrophoresis (dgge) of samples collected from the fermentation stages of the inyu production process. the dgge profiles targeted the bacterial 16s rdna and revealed the presence of citrobacter farmeri, enterobacter cloacae, enterobacter hormaechei, enterococcus faecium, klebsiella pneumoniae, pantoea agglomerans, salmonella enterica, ser ...201323200659
detection of cronobacter genus in powdered infant formula by enzyme-linked immunosorbent assay using anti-cronobacter antibody.cronobacter species (cronobacter spp.) are hazardous foodborne pathogens associated with baby food, powdered infant formula (pif). to develop a rapid and sensitive method for simultaneous detection of seven cronobacter spp. in pif, an indirect non-competitive enzyme-linked immunosorbent assay (inc-elisa) was developed based on a novel immunoglobulin g (igg), anti-cronobacter igg. the developed inc-elisa was able to detect seven cronobacter spp. at concentrations ranging from (5.6 ± 0.30) × 10(3) ...201627493642
re-examination of the taxonomic status of enterobacter helveticus, enterobacter pulveris and enterobacter turicensis as members of the genus cronobacter and their reclassification in the genera franconibacter gen. nov. and siccibacter gen. nov. as franconibacter helveticus comb. nov., franconibacter pulveris comb. nov. and siccibacter turicensis comb. nov., respectively.recently, a taxonomical re-evaluation of the genus enterobacter, based on multi-locus sequence typing (mlst) analysis, has led to the proposal that the species enterobacter pulveris, enterobacter helveticus and enterobacter turicensis should be reclassified as novel species of the genus cronobacter. in the present work, new genome-scale analyses, including average nucleotide identity, genome-scale phylogeny and k-mer analysis, coupled with previously reported dna-dna hybridization values and bio ...201425028159
rapid genus- and species-specific identification of cronobacter spp. by matrix-assisted laser desorption ionization-time of flight mass spectrometry.cronobacter spp. are gram-negative opportunistic food-borne pathogens and are known as rare but important causes of life-threatening neonatal infections. rapid and reliable identification of cronobacter species and their differentiation from phenotypically similar, nonpathogenic enterobacter turicensis, enterobacter helveticus, and enterobacter pulveris have become increasingly important. we evaluated here the application of matrix-assisted laser desorption ionization-time of flight mass spectro ...201020554814
enterobacter pulveris sp. nov., isolated from fruit powder, infant formula and an infant formula production environment.six gram-negative, facultatively anaerobic, non-spore-forming, coccoid rod-shaped isolates were obtained from fruit powder (n=3), infant formula (n=2) and an infant formula production environment (n=1) and investigated in a polyphasic taxonomic study. comparative 16s rrna gene sequence analysis combined with rpob gene sequence analysis allocated the isolates to the family enterobacteriaceae. the highest rpob gene sequence similarities (91.2-95.8%) were obtained with enterobacter helveticus, ente ...200818175715
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