| simple sequence repeat markers distinguish among morphotypes of sphaeropsis sapinea. | sphaeropsis sapinea is a fungal endophyte of pinus spp. that can cause disease following predisposition of trees by biotic or abiotic stresses. four morphotypes of s. sapinea have been described from within the natural range of the fungus, while only one morphotype has been identified on exotic pines in the southern hemisphere. the aim of this study was to develop robust polymorphic markers that could be used in both taxonomic and population studies. inter-short-sequence-repeat primers containin ... | 2001 | 11133466 |
| multiple gene genealogies and microsatellite markers reflect relationships between morphotypes of sphaeropsis sapinea and distinguish a new species of diplodia. | sphaeropsis sapinea is an opportunistic pathogen causing serious damage to conifers, pre-disposed by adverse environmental conditions or mechanical damage. three different morphological forms of the fungus have been described and are commonly referred to as the a, b and c morphotypes. isolates of the different morphotypes have also been separated based on differences in pathogenicity and molecular characteristics. these differences, however, overlap and have not been considered sufficiently robu ... | 2003 | 12884952 |
| apple polygalacturonase inhibiting protein1 expressed in transgenic tobacco inhibits polygalacturonases from fungal pathogens of apple and the anthracnose pathogen of lupins. | extracts from apple fruit (cultivar "granny smith") inhibited the cell-wall degrading polygalacturonase (pg) activity of colletotrichum lupini, the causal agent of anthracnose on lupins, as well as aspergillus niger pg. southern blot analysis indicated that this cultivar of apple has a small gene family of polygalacturonase inhibiting proteins (pgips), and therefore heterologous expression in transgenic tobacco was used to identify the specific gene product responsible for the inhibitory activit ... | 2006 | 16364381 |
| botryosphaeriaceae as potential pathogens of prunus species in south africa, with descriptions of diplodia africana and lasiodiplodia plurivora sp. nov. | botryosphaeriaceae are common dieback and canker pathogens of woody host plants, including stone fruit trees. in the present study the diversity of members of the botryosphaeriaceae isolated from symptomatic wood of prunus species (plum, peach, nectarine and apricot) was determined in stone fruit-growing areas in south africa. morphological and cultural characteristics as well as dna sequence data (5.8s rdna, its-1, its-2 and ef-1a) were used to identify known members and describe novel members ... | 2007 | 18268901 |
| detection of latent sphaeropsis sapinea infections in austrian pine tissues using nested-polymerase chain reaction. | abstract sphaeropsis sapinea is the causal agent of sphaeropsis tip blight disease of pines. past surveys of diseased and symptomless austrian and scots pines revealed that latent infections of symptomless shoots by s. sapinea are common. the role of these latent infections in the tip blight disease is unknown. a sampling technique and nested-polymerase chain reaction (pcr) protocol were developed to detect latent s. sapinea in symptomless pine shoots. the sampling protocol was designed to be mi ... | 2003 | 18943610 |
| differential analysis of mycoalexins in confrontation zones of grapevine fungal pathogens by ultrahigh pressure liquid chromatography/time-of-flight mass spectrometry and capillary nuclear magnetic resonance. | an original approach was developed for the chemical and biological investigation of zone lines formed by the confrontation of fungi growing in confined spaces. two wood-decaying fungi involved in esca disease, eutypa lata and botryosphaeria obtusa, were grown in petri dishes. metabolic profiles of pure fungal strains and confrontation zones were differentially analyzed by ultrahigh pressure liquid chromatography coupled to time-of-flight mass spectrometry (uhplc/tofms). selected metabolites indu ... | 2009 | 19182944 |
| rare or remarkable microfungi from oaxaca (south mexico)--part ii. | microfungi were collected in southern mexico in the vicinity of puerto escondido, oaxaca in 2007. in 2006, samples were gathered from acacia myrmecophytes [(remarkable microfungi from oaxaca of acacia species) part i]. in the present investigation [part ii], we collected microfungi from different parts of a variety of wild and cultivated higher plants belonging to the families anacardiaceae, caricaceae, fabaceae, moraceae, and nyctaginacae. the microfungi found here live as parasites or saprophy ... | 2008 | 19226752 |
| characterization and pathogenicity of botryosphaeriaceae species collected from olive and other hosts in spain and california. | species in the family botryosphaeriaceae are common pathogens causing fruit rot and dieback of many woody plants. in this study, 150 botryosphaeriaceae isolates were collected from olive and other hosts in spain and california. representative isolates of each type were characterized based on morphological features and comparisons of dna sequence data of three regions: internal transcribed spacer 5.8s, β-tubulin, and elongation factor. three main species were identified as neofusicoccum mediterra ... | 2010 | 20731532 |
| cytoplasmic- and extracellular-proteome analysis of diplodia seriata: a phytopathogenic fungus involved in grapevine decline. | abstract: | 2010 | 20828386 |
| a six locus phylogeny reveals high species diversity in botryosphaeriaceae from california almond. | botryosphaeriaceae are important pathogens on a variety of woody hosts, including almond, a major crop in california. almond is susceptible to botryosphaeria dothidea that forms band cankers on almond trunks, and the same fungus was also isolated from cankers of the canopy. to study the diversity and host range of b. dothidea and allied species from almond we used 132 isolates from 36 plant hosts from five continents, including 45 strains from almond in california. species were identified by com ... | 2010 | 20943551 |
| high-resolution melting analysis: a new molecular approach for the early detection of diplodia pinea in austrian pine. | the differentiation of diplodia pinea from closely related species, such as diplodia scrobiculata and diplodia seriata, and its detection in plant tissue, represented a critical issue for a long time. molecular screening tools have recently been developed to address this topic. in this study we applied one of the most sensitive and rapid diagnostic screening method so far developed, called high-resolution melting analysis (hrma), to detect d. pinea in austrian pine (pinus nigra). hrma exploits d ... | 2011 | 21802051 |