| phenotypic and molecular characterization of candida nivariensis sp. nov., a possible new opportunistic fungus. | the new species candida nivariensis, isolated from the clinical samples of three patients in spain over a 3-year period, is presented here. this species can be easily differentiated from candida glabrata, the closest genetic species, by different colony color on chromagar and by its ability to ferment trehalose. the analyses of the internal transcribed spacer region and the d1-d2 region of the 26s rrna gene sequences support a new species designation. | 2005 | 16081957 |
| pcr protocol for specific identification of candida nivariensis, a recently described pathogenic yeast. | candida nivariensis is a recently described pathogenic yeast closely related to candida glabrata. we developed a specific set of oligonucleotide primers based on the internal transcribed spacer regions of the rrna gene for the rapid identification of c. nivariensis. pcr with these primers amplified a 206-bp amplicon from c. nivariensis. | 2005 | 16333128 |
| catheter-related fungemia due to fluconazole-resistant candida nivariensis. | we report on a case of fungemia due to fluconazole-resistant candida nivariensis (mic, > or =128 microg/ml). internal transcribed spacer pcr followed by microchip gel electrophoresis with a blood culture that tested positive revealed a unique pattern different from those of other pathogenic yeasts. | 2007 | 17652473 |
| candida nivariensis isolated from an indonesian human immunodeficiency virus-infected patient suffering from oropharyngeal candidiasis. | candida nivariensis was isolated from an indonesian human immunodeficiency virus-infected patient who suffered from oropharyngeal candidiasis and was identified with molecular tools. our isolate demonstrated low mics to amphotericin b, flucytosine, posaconazole, caspofungin, and isavuconazole and was susceptible to fluconazole, itraconazole, and voriconazole. | 2008 | 18003796 |
| candida bracarensis detected among isolates of candida glabrata by peptide nucleic acid fluorescence in situ hybridization: susceptibility data and documentation of presumed infection. | molecular taxonomic studies have revealed new candida species among phenotypically delineated species, the best example being candida dubliniensis. this study was designed to determine the occurrence of two new molecularly defined species, candida bracarensis and candida nivariensis, which are closely related to and identified as candida glabrata by phenotypic assays. a total of 137 recent clinical isolates of c. glabrata identified by phenotypic characteristics was tested with c. bracarensis an ... | 2008 | 18077641 |
| candida nivariensis, an emerging pathogenic fungus with multidrug resistance to antifungal agents. | in 2005, candida nivariensis, a yeast species genetically related to candida glabrata, was described following its isolation from three patients in a single spanish hospital. between 2005 and 2006, 16 fungal isolates with phenotypic similarities to c. nivariensis were submitted to the united kingdom mycology reference laboratory for identification. the strains originated from various clinical specimens, including deep, usually sterile sites, from patients at 12 different hospitals in the united ... | 2008 | 18199788 |
| identification of candida nivariensis and candida bracarensis in a large global collection of candida glabrata isolates: comparison to the literature. | we analyzed 1,598 candida glabrata isolates for the presence of the cryptic species candida nivariensis and candida bracarensis. both species were very rare in this collection (0.2% prevalence), despite the number of isolates analyzed and the global distribution of the isolates. we saw no associated antifungal resistance in c. nivariensis. | 2009 | 19193845 |
| first isolations in india of candida nivariensis, a globally emerging opportunistic pathogen. | we randomly screened 363 yeast isolates during 2008 for their ability to form white colonies on chrom agar candida medium. two of these isolates (0.5%) were identified as candida nivariensis based on detailed phenotypic characterization and dna sequencing. one was recovered from the sputum of an hiv-positive patient with a pneumonic lesion and the second from the blood of a diabetic with oropharyngeal lesions. direct dna sequencing of the d1/d2 region of 28s rrna gene and/or the internal transcr ... | 2010 | 19626545 |
| a multiplex pcr protocol for rapid identification of candida glabrata and its phylogenetically related species candida nivariensis and candida bracarensis. | we have developed a multiplex pcr protocol for the detection of candida glabrata and its closely related species candida nivariensis and candida bracarensis. the method uses four pcr primers, targeting the its1 region and the 5.8s ribosomal rna gene. the combination of these primers yielded unique results to all candida species tested. the pcr assay we developed was found to be a rapid, specific and easy to perform method and it will be useful for characterizing large numbers of isolates for epi ... | 2009 | 19635503 |
| prevalence of candida bracarensis and candida nivariensis in a spanish collection of yeasts: comparison of results from a reference centre and from a population-based surveillance study of candidemia. | two new species related to candida glabrata, i.e., candida nivariensis and candida bracarensis, have been proposed. the occurrence of these species among isolates collected in a spanish mycology reference laboratory in 2008-2009 was reviewed. in addition, strains recovered as part of an active population-based surveillance of candidemia conducted in barcelona between 2002 and 2003 were also analyzed. among 143 clinical isolates received in 2008-2009, three (2%) were identified as c. bracarensis ... | 2011 | 21198347 |
| candida uthaithanina sp. nov., an anamorphic yeast species in nakaseomyces clade isolated in thailand. | three yeast stains were isolated from two unknown fruits (strains dd2-22-1(t) and sk44) and moss (strain st-449) in thailand. analysis of the d1/d2 domain of the large subunit (lsu) rrna gene sequences of the three strains revealed that they belonged to the same species. in terms of pairwise sequence similarity, candida cf. glabrata uwo(ps) 98-110.4 and candida nivariensis were the closest undescribed and recognized taxa, but the levels of nucleotide substitutions were 1.7-1.9% and 2.0-2.2%, res ... | 2011 | 21311971 |
| prevalence and antifungal susceptibility patterns of new cryptic species inside the species complexes candida parapsilosis and candida glabrata among blood isolates from a spanish tertiary hospital. | objectives there is scarce information on the clinical relevance and antifungal susceptibility of candida bracarensis, candida nivariensis, candida orthopsilosis and candida metapsilosis. the objective of this study was to assess the prevalence and in vitro antifungal susceptibility of these cryptic species among 173 blood isolates previously identified as candida glabrata or candida parapsilosis at the hospital of cruces (barakaldo, spain). the survey was extended to 518 clinical isolates from ... | 2011 | 21795259 |
| rapid discrimination between candida glabrata, candida nivariensis and candida bracarensis using a simple-plex pcr. | we report here a pcr-based assay using a single primer pair targeting the rpl31 gene that allows the discrimination between candida glabrata, c. bracarensis and c. nivariensis according to the size of the generated amplicon. | 2011 | 21752976 |
| digestive fungal flora in asymptomatic subjects in bobo-dioulasso, burkina faso. | to identify candida species in asymptomatic subjects in bobo-dioulasso (burkina faso) by the matrix-assisted laser desorption ionization-time of flight mass spectrometry. | 2014 | 25183337 |
| rapid and accurate identification of isolates of candida species by melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (its2-mca). | rapid identification of clinically important yeasts can facilitate the initiation of anti-fungal therapy, since susceptibility is largely species-dependent. we evaluated melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (its2-mca) as an identification tool for distinguishing between 16 candida spp., i.e. candida albicans, candida bracarensis, candida dubliniensis, candida famata, candida glabrata, candida guilliermondii, candida inconspicua, candida k ... | 2013 | 23142490 |
| epidemiology, species distribution and in vitro antifungal susceptibility of fungaemia in a spanish multicentre prospective survey. | to update the knowledge of the epidemiology of fungaemia episodes in spain, the species implicated and their in vitro antifungal susceptibilities. | 2012 | 22351683 |
| evaluation of the commercial rapid trehalose test (glabrata rtt) for the point of isolation identification of candida glabrata isolates in primary cultures. | candidaemias account for 10-20% of nosocomial bloodstream infections depending on the study. whilst candida albicans remains the most frequently isolated species, candida glabrata may be responsible for as many as 10-25% of all candidaemias. moreover, c. glabrata is generally less susceptible to the azole antifungals than the majority of other pathogenic yeast species. thus, a rapid test for the specific identification of isolates of c. glabrata would be useful for patient management if it could ... | 2012 | 22234529 |
| identification and antifungal susceptibility profiles of candida nivariensis and candida bracarensis in a multi-center chinese collection of yeasts. | candida nivariensis and c. bracarensis are two emerging cryptic species within the c. glabrata complex. thirteen of these isolates from 10 hospitals in china were studied for their species identification and antifungal susceptibilities. phenotypic and molecular [rdna its sequencing, d1/d2 sequencing and its sequencer-based capillary gel electrophoresis (scge)] and matrix-assisted laser desorption ionization-time of flight (maldi-tof) ms identification methods were compared for their performance ... | 2017 | 28154553 |
| multiplex pcr designed to differentiate species within the candida glabrata complex. | no phenotypic methods are available to unequivocally differentiate species within the candida glabrata complex. | 2017 | 27810263 |
| candida glabrata species complex prevalence and antifungal susceptibility testing in a culture collection: first description of candida nivariensis in argentina. | the presence of the cryptic species belonging to the candida glabrata complex has not been studied in argentina. we analyzed a collection of 117 clinical isolates of c. glabrata complex belonging to a national culture collection of instituto nacional de microbiología "dr. carlos g. malbrán" from argentina (40 isolates from blood samples, 18 from other normally sterile sites, 20 from vagina, 14 from urine, 7 from oral cavity, 3 from catheter, 1 from a stool sample and 14 isolates whose clinical o ... | 2016 | 27681573 |
| in vitro fungicidal activities of anidulafungin, caspofungin, and micafungin against candida glabrata, candida bracarensis, and candida nivariensis evaluated by time-kill studies. | anidulafungin, caspofungin, and micafungin killing activities against candida glabrata, candida bracarensis, and candida nivariensis were evaluated by the time-kill methodology. the concentrations assayed were 0.06, 0.125, and 0.5 μg/ml, which are achieved in serum. anidulafungin and micafungin required between 13 and 26 h to reach the fungicidal endpoint (99.9% killing) against c. glabrata and c. bracarensis. all echinocandins were less active against c. nivariensis. | 2015 | 25801575 |
| candida nivariensis in comparison to different phenotypes of candida glabrata. | the purpose of the study was to establish the prevalence of new candida glabrata complex species: candida nivariensis and candida bracarensis isolated from clinical material, evaluate their phenotypes and the prevalence of gene family encoding extracellular glycosylphosphatidylinositol-linked aspartyl proteases, crucial for c. glabrata virulence. study material included 224 c. glabrata clinical strains. candida glabrata phenotypes were identified using chromagar candida medium. strains were anal ... | 2014 | 25267284 |
| prevalence of candida nivariensis and candida bracarensis in vulvovaginal candidiasis. | candida nivariensis and candida bracarensis were isolated from patients with vulvovaginal candidiasis (vvc). candida nivariensis and candida bracarensis were found in presumptive candida glabrata isolates, which were identified using the api candida system. we retrospectively re-examined vaginal presumptive candida glabrata isolates for candida nivariensis and candida bracarensis from january 1, 2003, through december 31, 2012, via detection of the its1 region and the 5.8s ribosomal rna gene. am ... | 2014 | 25118875 |
| [first case report of catheter-related fungemia by candida nivariensis in the iberian peninsula]. | in recent years the incidence of candidemia caused by non-albicans candida species has been increasing. two cryptic species have been described within the candida glabrata complex, candida nivariensis and candida bracarensis, which may be troublesome in laboratory identification and have lower susceptibility to fluconazole. | 2013 | 22982698 |
| validation of the pcr-dhplc method for rapid identification of candida glabrata phylogenetically related species in different biological matrices. | since two new species phylogenetically related to candida glabrata with slightly different phenotypes and antifungal susceptibility profiles have been described, it seems to be necessary from clinical point of view, to develop a rapid and accurate identification system in order to distinguish between these three fungal species. we studied the performance of denaturing high performance liquid chromatography (dhplc) as a faster (less than 7 min) and alternative novel technique for simultaneous ana ... | 2012 | 22456535 |
| candida nivariensis as a new emergent agent of vulvovaginal candidiasis: description of cases and review of published studies. | candida nivariensis is a new emergent agent related to human infections in the vaginal tract and other localizations, but the phenotypic characteristics are very similar to candida glabrata and can be misidentified and underdiagnosed. we described four cases of vulvovaginitis identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and confirmed the results with pcr amplification and sequencing of the entire its genomic region (its1, its2 and 5.8 rrna). we reinf ... | 2016 | 26708316 |
| occurrence and characterization of candida nivariensis from a culture collection of candida glabrata clinical isolates in malaysia. | candida nivariensis and c. bracarensis have been recently identified as emerging yeast pathogens which are phenotypically indistinguishable from c. glabrata. however, there is little data on the prevalence and antifungal susceptibilities of these species. | 2014 | 25022264 |
| candida nivariensis isolated from a renal transplant patient with persistent candiduria-molecular identification using its pcr and maldi-tof. | we report on the isolation of candida nivariensis from a renal transplant patient with persistent candiduria. biochemical profiling misidentified isolates as candida glabrata (3/5) and candida inconspicua (2/5). all isolates produced white colonies on chromagar(™) candida medium. internal transcribed spacer (its) ribosomal gene sequence analysis and maldi-tof-ms analysis (bruker biotyper(™) 2.0) identified all isolates as c. nivariensis, demonstrating the utility of maldi-tof as a rapid, accurat ... | 2013 | 24432244 |
| candida nivariensis as an etiologic agent of vulvovaginal candidiasis in a tertiary care hospital of new delhi, india. | candida nivariensis is a cryptic species, phenotypically indistinguishable from candida glabrata and identified by molecular methods. aside its isolation from broncho-alveolar lavage, we report for the first time the etiologic role of c. nivariensis in 4 patients with vulvovaginal candidiasis. of 100 phenotypically identified c. glabrata isolates originating from vaginal swabs, 4 were identified as c. nivariensis by polymerase chain reaction and confirmed by sequencing. all of the c. nivariensis ... | 2013 | 23537782 |
| first description of candida nivariensis in brazil: antifungal susceptibility profile and potential virulence attributes. | this study evaluated the antifungal susceptibility profile and the production of potential virulence attributes in a clinical strain of candida nivariensis for the first time in brazil, as identified by sequencing the internal transcribed spacer (its)1-5.8s-its2 region and d1/d2 domains of the 28s of the rdna. for comparative purposes, tests were also performed with reference strains. all strains presented low planktonic minimal inhibitory concentrations (pmics) to amphotericin b (amb), caspofun ... | 2016 | 26814644 |
| diversity of culturable yeasts in phylloplane of sugarcane in thailand and their capability to produce indole-3-acetic acid. | yeasts were isolated by the enrichment technique from the phylloplane of 94 samples of sugarcane leaf collected from seven provinces in thailand. all sugarcane leaf samples contained yeasts and 158 yeast strains were obtained. on the basis of the d1/d2 domain of the large subunit rrna gene sequence analysis, 144 strains were identified to 24 known species in 14 genera belonging to the ascomycota viz. candida akabanensis, candida dendronema, candida mesorugosa, candida michaelii, candida nivarien ... | 2014 | 24442819 |
| looking for candida nivariensis and c. bracarensis among a large italian collection of c. glabrata isolates: results of the fimua working group. | two recently described pathogenic candida species, c. nivariensis and c. bracarensis, share many phenotypic characteristics with c. glabrata and are easily misidentified as such. the aim of this study was to determine the occurrence of these cryptic species in italy. one thousand yeast isolates collected in 14 italian regions and identified as c. glabrata by phenotypic and biochemical methods were included in this study: 928 were screened on chromagar and 72 were analysed by a multiplex pcr. non ... | 2013 | 23170902 |