| characterization of italian cheeses ripened under nonconventional conditions. | four italian cheeses (casciotta di urbino, barricato san martino, vento d'estate, and ubriaco di raboso) nonconventionally ripened under different plant materials (walnut leaves, herbs, hay, and wine by-products, respectively) were compared for compositional, microbiological, biochemical, and volatile profile characteristics. mean values for gross composition were rather similar. because primary starters were not used for manufacture, the endogenous lactic acid bacteria were mainly present (7.0 ... | 2007 | 17517708 |
| enterococcus thailandicus sp. nov., isolated from fermented sausage ('mum') in thailand. | a gram-positive and catalase-negative coccus that formed chains, designated strain fp48-3(t), isolated from fermented sausage ('mum'), was studied systematically. strain fp48-3(t) was facultatively anaerobic and produced l-lactic acid from glucose. straight-chain fatty acids c(18 : 1) and c(16 : 0) were the dominant components. the dna g+c content of strain fp48-3(t) was 37.9 mol%. on the basis of 16s rrna and rna polymerase alpha-subunit (rpoa) gene sequence analysis, strain fp48-3(t) was close ... | 2008 | 18599707 |
| designation of the provisional new enterococcus species cdc pns-e2 as enterococcus sanguinicola sp. nov., isolated from human blood, and identification of a strain previously named enterococcus cdc pns-e1 as enterococcus italicus fortina, ricci, mora, and manachini 2004. | we have previously characterized two new enterococcal species (provisionally designated cdc pns-e1 and cdc pns-e2) recovered from clinically significant specimens associated with invasive infections in humans. in the present report we provide additional data and propose formal denominations for isolates of these two species of enterococcus. results of 16s rrna gene sequencing, sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis of whole-cell protein profiles, and dna-dna reassocia ... | 2008 | 18667594 |
| identification and tracing of enterococcus spp. by rapd-pcr in traditional fermented sausages and meat environment. | four local small-scale factories were studied to determine the sources of enterococci in traditional fermented sausages. | 2009 | 19120623 |
| polyphasic taxonomic studies of lactic acid bacteria associated with tunisian fermented meat based on the heterogeneity of the 16s-23s rrna gene intergenic spacer region. | the objective of this work was to investigate the structure and diversity of lactic acid bacteria (lab) communities in traditionally fermented meat collected from different areas of tunisia. a polyphasic study, which involves phenotypic tests and ribosomal dna-based techniques, was used to identify gram-positive and catalase-negative isolates. pcr amplification of the 16s-23s rdna isr of 102 isolates and other reference lab strains gave (1) one type of rrn operon (m-isr) for lactococci, (2) two ... | 2009 | 19669730 |
| reevaluation of the taxonomic status of recently described species of enterococcus: evidence that enterococcus thailandicus is a senior subjective synonym of "enterococcus sanguinicola", and confirmation of enterococcus caccae as a species distinct from enterococcus silesiacus. | several of the more recently proposed new species of enterococcus are nearly identical based on 16s rdna sequence analysis and phenotypic traits. in the present study, dna-dna reassociation experiments, in conjunction with sequencing of the 16s rrna and rpob genes, provided evidence that "enterococcus sanguinicola" and enterococcus thailandicus actually represent the same species. in contrast, enterococcus caccae and enterococcus silesiacus, two other species with nearly identical 16s rrna gene ... | 2011 | 21543565 |
| reevaluation of the taxonomic status of recently described species of enterococcus: evidence that enterococcus thailandicus is a senior subjective synonym of "enterococcus sanguinicola", and confirmation of enterococcus caccae as a species distinct from enterococcus silesiacus. | several of the more recently proposed new species of enterococcus are nearly identical based on 16s rdna sequence analysis and phenotypic traits. in the present study, dna-dna reassociation experiments, in conjunction with sequencing of the 16s rrna and rpob genes, provided evidence that "enterococcus sanguinicola" and enterococcus thailandicus actually represent the same species. in contrast, enterococcus caccae and enterococcus silesiacus, two other species with nearly identical 16s rrna gene ... | 2011 | 21543565 |
| phylogenetic analysis of antimicrobial lactic acid bacteria from farmed seabass dicentrarchus labrax. | the use of lactic acid bacteria (lab) in the prevention or reduction of fish diseases is receiving increasing attention. in the present study, 47 lab strains were isolated from farmed seabass ( dicentrarchus labrax ) and were phenotypically and phylogenetically analysed by 16s rdna and randomly amplified polymorphic dna - polymerase chain reaction (rapd-pcr). their antimicrobial effect was tested in vitro against a wide variety of pathogenic and spoilage bacteria. most of the strains isolated we ... | 2012 | 22439634 |
| isolation and taxonomic identity of bacteriocin-producing lactic acid bacteria from retail foods and animal sources. | bacteriocin-producing (bac⁺) lactic acid bacteria (lab) were isolated from a variety of food products and animal sources. samples were enriched in de man, rogosa, and sharpe (mrs) lactocilli broth and plated onto mrs agar plates using a "sandwich overlay" technique. inhibitory activity was detected by the "deferred antagonism" indicator overlay method using listeria monocytogenes as the primary indicator organism. antimicrobial activity against l. monocytogenes was detected by 41 isolates obtain ... | 2015 | 27682080 |
| comparative genomics of enterococcus spp. isolated from bovine feces. | enterococcus is ubiquitous in nature and is a commensal of both the bovine and human gastrointestinal (gi) tract. it is also associated with clinical infections in humans. subtherapeutic administration of antibiotics to cattle selects for antibiotic resistant enterococci in the bovine gi tract. antibiotic resistance genes (args) may be present in enterococci following antibiotic use in cattle. if located on mobile genetic elements (mges) their dissemination between enterococcus species and to pa ... | 2017 | 28270110 |
| effect of in-feed administration and withdrawal of tylosin phosphate on antibiotic resistance in enterococci isolated from feedlot steers. | tylosin phosphate is a macrolide commonly administered to cattle in north america for the control of liver abscesses. this study investigated the effect of in-feed administration of tylosin phosphate to cattle at subtherapeutic levels and its subsequent withdrawal on macrolide resistance using enterococci as an indicator bacterium. fecal samples were collected from steers that received no antibiotics and steers administered tylosin phosphate (11 ppm) in-feed for 197 days and withdrawn 28 days be ... | 2015 | 26074889 |
| virulence factors genes in enterococci isolated from beavers (castor fiber). | only limited information exists concerning the microbiota in beaver (castor fiber). this study has been focused on the virulence factors genes detection in enterococci from beavers. in general, animals are not affected by enterococcal infections, but they can be a reservoir of, e.g. pathogenic strains. moreover, detection of virulence factors genes in enterococci from beavers was never tested before. free-living beavers (12), male and female (age 4-5 years) were caught in the north-east part of ... | 2015 | 25300356 |
| selection and characterization of cheonggukjang (fast fermented soybean paste)-originated bacterial strains with a high level of s-adenosyl-l-methionine production and probiotics efficacy. | this study was executed to develop probiotics producing s-adenosyl-l-methionine (same), a methyl group donor in the 5-methyltetrahydrofolate methylation reaction in animal cells. same is an essential substance in the synthesis, activation, and metabolism of hormones, neurotransmitters, nucleic acids, phospholipids, and cell membranes of animals. same is also known as a nutritional supplement for improving human brain function. in this study, same-producing strains were identified in six kinds of ... | 2014 | 25268945 |
| transferable multiresistance plasmids carrying cfr in enterococcus spp. from swine and farm environment. | seventy-seven porcine enterococcus isolates with florfenicol mics of ≥16 μg of were/ml screened for the presence of the multiresistance gene cfr, its location on plasmids, and its genetic environment. three isolates-enterococcus thailandicus 3-38 (from a porcine rectal swab collected at a pig farm), enterococcus thailandicus w3, and enterococcus faecalis w9-2 (the latter two from sewage at a different farm), carried the cfr gene. the smai pulsed-field gel electrophoresis patterns of the three is ... | 2013 | 23070165 |
| a microplate growth inhibition assay for screening bacteriocins against listeria monocytogenes to differentiate their mode-of-action. | lactic acid bacteria (lab) have historically been used in food fermentations to preserve foods and are generally-recognized-as-safe (gras) by the fda for use as food ingredients. in addition to lactic acid; some strains also produce bacteriocins that have been proposed for use as food preservatives. in this study we examined the inhibition of listeria monocytogenes 39-2 by neutralized and non-neutralized bacteriocin preparations (bac+ preps) produced by lactobacillus curvatus fs47; lb. curvatus ... | 2015 | 26111195 |
| screening and characterization of lab-produced bacteriocin-like substances from the intestine of grey mullet (mugil cephalus l.) as potential biocontrol agents in aquaculture. | to isolate and characterize bacteriocin-like inhibitory substance (blis)-producing lactic acid bacteria from the intestine of grey mullet. | 2013 | 23075097 |
| draft genome sequence of an enterococcus thailandicus strain isolated from bovine feces. | here, we report the first draft genome sequence of enterococcus thailandicus isolated from the feces of feedlot cattle in southern alberta. | 2016 | 27445365 |
| identification and characterization of lactic acid bacteria isolated from traditional pickles in sichuan, china. | the pickle, a traditional fermented vegetable product, is popular in sichuan province of china. the objective of this study was to investigate the diversity of dominant lactic acid bacteria (lab) in pickles by analyzing 36 samples collected from 6 different regions in sichuan province. the lab counts in these samples varied from 3.90 to 8.40 log cfu ml(-1). in total, 185 presumptive lab with gram-positive and catalase-negative properties were obtained from these samples using mrs agar, and those ... | 2012 | 22878734 |