rapid fixation and embedding method for immunocytochemical studies of tomato spotted wilt tospovirus (tswv) in plant and insect tissues.a new rapid fixation and embedding technique using microwave energy was evaluated for immunolabelling and examination of ultrastructure of plant and insect cells. tissues in gluteraldehyde-paraformaldehyde were fixed for fifteen seconds in a microwave at 100% power, and dehydrated. microwave energy was then used to polymerize the london resin white (lr white) acrylic resin during the embedding process. embedded specimens were then thin sectioned (90 nm) and treated with anti-tomato spotted wilt ...19938490237
in vitro cytotoxic and antitumor property of emilia sonchifolia (l.) dc in mice.methanolic extract of emilia sonchifolia (compositae), a folklore medicinal plant, was found to be cytotoxic to daltons lymphoma (dl), ehrlich ascites carcinoma (eac) and mouse lung fibroblast (l-929) cells, but not toxic to normal human lymphocytes, under in vitro conditions. oral administration of the extract (100 mg/kg, b. wt) to mice reduced the development of both solid and ascites tumors and increased the life span of these tumor bearing mice. further, the extract inhibited dna synthesis a ...200011091004
a preliminary study on the anti-inflammatory properties of emilia sonchifolia leaf extracts.the aqueous and methanolic extracts of emilia sonchifolia leaves progressively reduced rat paw oedema induced by subplantar injection of albumin, the aqueous extract showing a more pronounced effect than the methanol extract.200011449473
the route of tomato spotted wilt virus inside the thrips body in relation to transmission efficiency.the route of tomato spotted wilt virus (tswv) in the body of its vectors, frankliniella occidentalis and thrips tabaci (thysanoptera: thripidae) was studied during their development. first instar larvae were allowed, immediately upon hatching, to acquire virus from mechanically infected datura stramonium plants for 24 h. the rate of transmission by adults was determined in inoculation access feeding test on emilia sonchifolia leaf disks. thrips tissues were analysed for infection at 24 h interva ...200212417949
modulation of selenite cataract by the flavonoid fraction of emilia sonchifolia in experimental animal models.the purpose of the study was to investigate the role of flavonoids from emilia sonchifolia (es) on the progression of selenite-induced cataract. the antioxidant property of the flavonoids isolated from es was assessed by measuring its capacity to inhibit superoxide production and serum oxidation in vitro in comparison with quercetin. based on these experiments, an in vivo study was conducted to evaluate the modulatory effects of the flavonoids against selenite cataract. cataract was induced by a ...200617009203
effects of temperature and host on the generation of tomato spotted wilt virus defective interfering rnas.abstract the generation of defective interfering (di) rna molecules of tomato spotted wilt tospovirus (tswv) was studied by serially passaging in-ocula from plant to plant under different controlled conditions. di rnas were generated at higher rates in plants at 16 degrees c than in plants incubated at higher temperatures. another factor promoting the tswv di rna generation was the use of high virus concentrations in the inocula. the solanaceous species capsicum annuum, datura stramonium, lycope ...199718945014
death receptor 5-mediated tnfr family signaling pathways modulate γ-humulene-induced apoptosis in human colorectal cancer ht29 cells.a component from emilia sonchifolia (l.) dc, γ-humulene, was investigated. significantly decreased cell viability of human colorectal cancer ht29 cells in a dose-dependent manner with ic50 53.67±2.99 μm for 24-h treatment was found. γ-humulene induced apoptotic cell death and apoptosis was confirmed by morphological assessment. the staining with propidium iodide (pi) and flow cytometric analysis also showed that γ-humulene significantly promoted the sub-g1 phase (an apoptotic population) in ht29 ...201021152878
antinociceptive effect of extract of emilia sonchifolia in mice.emilia sonchifolia (l.) dc. (asteraceae) is a medicinal plant traditionally used in brazilian folk medicine to treat asthma, fever, cuts, wounds and rheumatism. this study was conducted to establish the antinociceptive properties of hydroethanolic extract from aerial parts of emilia sonchifolia in mice using chemical and thermal models of nociception.201021185930
effect of emilia sonchifolia (linn.)dc on alcohol- induced oxidative stress in pancreas of male albino explore the efficacy of n-hexane extract of emilia sonchifolia (e. sonchifobia) against ethanol induced pancreatic dysfunction in the young wistar albino rats.201122118034
antimicrobial activity of emilia sonchifolia dc., tridax procumbens l. and vernonia cinerea l. of asteracea family: potential as food preservatives.chemical preservatives have been used in the food industry for many years. however, with increased health concerns, consumers prefer additive-free products or food preservatives based on natural products. this study evaluated antimicrobial activities of extracts from emilia sonchifolia l. (common name: lilac tassel flower), tridax procumbens l. (common name: tridax daisy) and vernonia cinerea l. (common name: sahadevi), belonging to the asteracea family, to explore their potential for use agains ...200922691820
emilia sonchifolia extract activity against white spot syndrome virus and yellow head virus in shrimp cell cultures.emilia sonchifolia (l.) dc is a plant used in traditional medicine to treat several viral and bacterial diseases. the antiviral activities of selected sephadex lh-20 column fractions and hplc subfractions of an acetone extract of e. sonchifolia leaves were determined in shrimp penaeus merguiensis primary lymphoid cells infected with either white spot syndrome virus (wssv) or yellow head virus (yhv). wssv and yhv replication was quantified using quantitative real-time pcr tests targeted to the vp ...201526203887
performance of bemisia tabaci (genn.) biotype b (hemiptera: aleyrodidae) on weeds.bemisia tabaci (genn.) biotype b (hemiptera: aleyrodidae) is regarded as a pest with a large number of hosts, including crops and weeds. the performance of this whitefly on seven weeds was evaluated in order to identify the most suitable host. the following weeds that are very common in intense agricultural areas in the state of são paulo, brazil, were selected for this study: spurge (euphorbia heterophylla), beggarticks (bidens pilosa), red tasselflower (emilia sonchifolia), small-flower galins ...201427194066
selection of nectar plants for use in ecological engineering to promote biological control of rice pests by the predatory bug, cyrtorhinus lividipennis, (heteroptera: miridae).ecological engineering for pest management involves the identification of optimal forms of botanical diversity to incorporate into a farming system to suppress pests, by promoting their natural enemies. whilst this approach has been extensively researched in many temperate crop systems, much less has been done for rice. this paper reports the influence of various plant species on the performance of a key natural enemy of rice planthopper pests, the predatory mirid bug, cyrtorhinus lividipennis. ...201425254377
evaluation of antiangiogenic efficacy of emilia sonchifolia (l.) dc on tumor-specific neovessel formation by regulating mmps, vegf, and proinflammatory cytokines.formation of new blood vessels from preexisting vasculature is an indispensable process in tumor initiation, invasion, and metastasis. novel therapeutic approaches target endothelial cells involved in the process of angiogenesis, due to their genetic stability relative to the rapidly mutating drug-resistant cancer cells. in the present study, we investigated the effect of an active fraction from emilia sonchifolia, belonging to the family asteraceae, a plant well known for its anti-inflammatory ...201627146127
inhibition of pulmonary metastasis by emilia sonchifolia (l.) dc: an in vivo experimental study.emilia sonchifolia (l.) dc is a widely distributed medicinal herb used mainly in the indigenous ayurvedic system of medicine in india. this plant is one among the ten sacred plants of kerala state in india, collectively known as dasapushpam.201626926173
immune response modulatory effect of emilia sonchifolia (l.) dc: an in vivo experimental study.this study was performed in order to provide a scientific basis for the conventional use of emilia sonchifolia in the traditional indian ayurvedic medicine possibly through modulation of the host immune defense.201526280169
[chemical constituents contained in aerial parts of emilia sonchifolia].to study the chemical constituents contained in ethanol extracts from aerial parts of emilia sonchifolia.201223397723
a new cyclohexylacetic acid derivative from the aerial parts of emilia sonchifolia.a new cyclohexylacetic acid derivative, named 2-{4-hydroxy-7-oxabicyclo [2.2.1] heptanyl}-acetic acid (1), was isolated from emilia sonchifolia, together with a known analogue, 2-(1,4-dihydroxy cyclohexanyl)-acetic acid (2). their structures were determined on the basis of spectroscopic techniques including ir, nmr, hr-esi-ms and x-ray diffraction.201323163244
inhibition of pro-inflammatory cytokines and inducible nitric oxide by extract of emilia sonchifolia l. aerial parts.emilia sonchifolia l. (asteraceae) is used in ethnomedicine for the treatment of a wide array of inflammatory disorders. this practice has also been supported by scientific reports which showed that extracts of e. sonchifolia possess anti-inflammatory effects in rodents. however, the mechanism(s) through which the extracts produce these effects is not known. in this study, the effect of a methanol/methylene chloride extract of e. sonchifolia (es) on the levels of il-1β and tnf-α after an intrape ...201222712801
activations of both extrinsic and intrinsic pathways in hct 116 human colorectal cancer cells contribute to apoptosis through p53-mediated atm/fas signaling by emilia sonchifolia extract, a folklore medicinal plant.emilia sonchifolia (l.) dc (compositae), an herbaceous plant found in taiwan and india, is used as folk medicine. the clinical applications include inflammation, rheumatism, cough, cuts fever, dysentery, analgesic, and antibacteria. the activities of emilia sonchifolia extract (ese) on colorectal cancer cell death have not been fully investigated. the purpose of this study explored the induction of apoptosis and its molecular mechanisms in ese-treated hct 116 human colorectal cancer cells in vit ...201222474491
protective effect of emilia sonchifolia (l.) against high protein diet induced oxidative stress in pancreas of wistar rats.the present study was undertaken to investigate the oxidative damage, the biochemical and histopathological changes in the pancreas of the wistar rats which was fed high protein diet and the recovery after the oral administration of the n-hexane extract of the herb, emilia sonchifolia.201222368400
in vitro antioxidant activity and hptlc determination of n-hexane extract of emilia sonchifolia (l.)dc.the free radical scavenging activities of n-hexane extract of the whole plant of emilia sonchifolia was evaluated by employing various in vitro assay systems like dpph radical scavenging activity, superoxide radical scavenging activity and hydrogen peroxide scavenging activity with ic50 values 180, 160 and 160 μg/ml respectively. the results of the study indicate that the n-hexane extract of the whole plant of emilia sonchifolia possess a significant scavenging effect with increasing concentrati ...201124826021
fire control - a conservation tool for certain medical plants in grass hills ecosystem, the western ghats.grass hills ecosystem lies in anaimalais. the western ghats possesses rich biodiversity, the annual summer fire, an integral part of this ecosystem, promotes the ecological status of certain perennial grasses including the dominant grass. chrysopogon zeylanicus thw. on the other hand, some medicinal plants viz., impatiens tomentosa heyne, drosera peltata sm osbeckia parviflora arn., emilia sonchifolia dc. lecanthus penduncularis wedd. and lobelia nicotianifolia heyne were identiflora arn. emilia ...200122557019
[chemical constituents of emilia sonchifolia l. dc].five known compounds were obtained from the whole plant of emilia sonchifolia. by means of chemical and spectral methods, they were identified to be simiral, beta-sitosterol, stigmasterol, palmitic acid and honey acid.19938323691
[pyrrolizidine alkaloids from emilia sonchifolia.].the pyrrolizidine alkaloids, senkirkine ( 1) and doronine ( 2) were isolated from the aerial parts of emilia sonchifolia.198617345415
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