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paenibacillus humicus sp. nov., isolated from poultry litter compost.two bacterial strains, pc-142 and pc-147(t), isolated from poultry litter compost, were characterized with respect to their phenetic and phylogenetic characteristics. the isolates were endospore-forming rods that were reddish in colour after gram staining. they were catalase- and oxidase-positive, were able to degrade starch and gelatin and grew at 15-40 degrees c and ph 5.5-10.0. the predominant fatty acids were anteiso-c(15 : 0), iso-c(15 : 0) and iso-c(16 : 0), the major respiratory quinone w ...200717911294
identification of novel hora-harbouring bacteria capable of spoiling beer.an atp-binding cassette (abc) multi-drug resistance (mdr) gene was found in 4 gram-positive bacterial isolates of environmental origin and found capable of spoiling beer. the bacteria isolated were bacillus cereus, bacillus licheniformis, paenibacillus humicus, and staphylococcus epidermidis; all of which were previously unappreciated as beer-spoilage bacteria. the mdr gene found in these bacteria has less than 37% similarity to known abc mdr proteins described for bacillus and staphylococcus, a ...200818389005
broad-range pcr, cloning and sequencing of the full 16s rrna gene for detection of bacterial dna in synovial fluid samples of tunisian patients with reactive and undifferentiated arthritis.broad-range rdna pcr provides an alternative, cultivation-independent approach for identifying bacterial dna in reactive and other form of arthritis. the aim of this study was to use broad-range rdna pcr targeting the 16s rrna gene in patients with reactive and other forms of arthritis and to screen for the presence of dna from any given bacterial species in synovial fluid (sf) samples.200919570210
staphylococcus aureus and zygosaccharomyces bailii as primary microbial contaminants of a spoiled herbal food supplement and evaluation of their survival during shelf life.this investigation was carried out to identify the microbiota in a spoiled commercial food supplement consisting of a syrup suspension of a mixture of dried herbs and herb extracts. the product did not contain alkyl-p-hydroxybenzoates (parabens) as preservatives, was kept at room temperature and showed abundant gas formation. colonies of distinct morphology were recovered on bacteria- and yeast-specific media, and tested for their ability to grow in the product. genetic differentiation and ident ...201020227600
paenibacillus wooponensis sp. nov., isolated from wetland freshwater.a rod-shaped enodospore-forming gram-positive bacterium, strain wpcb018t, was isolated from a fresh water sample collected from woopo wetland, korea. the isolate was identified as a member of the genus paenibacillus on the basis of phenotypic characteristics and phylogenetic inference based on 16s rrna gene sequence. the major polar lipids are diphosphatidylglycerol, phosphatidylethanolamine and unknown aminophospholipids. the diamino acid found in the cell wall peptidoglycan is meso-diaminopime ...201121216920
application of chimeric glucanase comprising mutanase and dextranase for prevention of dental biofilm formation.water-insoluble glucan (wig) produced by mutans streptococci, an important cariogenic pathogen, plays an important role in the formation of dental biofilm and adhesion of biofilm to tooth surfaces. glucanohydrolases, such as mutanase (α-1,3-glucanase) and dextranase (α-1,6-glucanase), are able to hydrolyze wig. the purposes of this study were to construct bi-functional chimeric glucanase, composed of mutanase and dextranase, and to examine the effects of this chimeric glucanase on the formation ...201525411090
gene cloning, expression, and characterization of mutanase from paenibacillus curdlanolyticus mp-1.mutanases hydrolyze d-glucosidic linkages of α-1,3-linked polysaccharides which are important components of dental plaque. therefore, these enzymes can be useful in preventive oral hygiene. a gene encoding mutanase was cloned from soil-isolated paenibacillus curdlanolyticus mp-1 and expressed in escherichia coli, and the resulting recombinant enzyme was characterized. the nucleotide sequence of the mutanase gene consisted of 3786 nucleotides encoding a protein of 1261 amino acids with a theoreti ...201222982219
paenibacillus herberti sp. nov., an endophyte isolated from herbertus sendtneri.strain r33(t), an endophyte recovered from herbertus sendtneri, was identified as representing a novel species of the genus paenibacillus by using a polyphasic taxonomic approach. the novel strain was observed to be a gram-stain positive, aerobic, rod-shaped, motile and endospore-forming bacterium. the major polar lipids of strain r33(t) were identified as diphosphatidylglycerol, phosphatidylethanolamine, along with lesser amounts of phosphatidylglycerol, three unidentified aminophospholipids, t ...201526155771
draft genome sequence of the growth-promoting endophyte paenibacillus sp. p22, isolated from populus.paenibacillus sp. p22 is a gram-negative facultative anaerobic endospore-forming bacterium isolated from poplar hybrid 741 (♀[populus alba × (p. davidiana + p. simonii) × p. tomentosa]). this bacterium shows strong similarities to paenibacillus humicus, and important growth-promoting effects on in vitro grown explants of poplar hybrid 741 have been described.201424723717
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