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[phylogenic diversity of soil chemotatic bacteria in a phosphorous-rich area around dianchi lake of yunnan province, southwest china].phosphate-solubilizing bacteria (psb) were isolated from 100 soil samples collected from a phosphorous-rich area around the dianchi lake of yunnan province, southwest china. the chemotatic psb strains were screened by chea gene detection, and their chemotaxis was verified by the method of soft agar plate. the tricalcium phosphate (tcp)-solubilizing activities of psb were determined with molybdenum blue spectrophotometry. based on 16s rrna sequences, the phylogenic relationships of the psb were a ...201223173478
plant growth promoting bacteria from crocus sativus rhizosphere.present study deals with the isolation of rhizobacteria and selection of plant growth promoting bacteria from crocus sativus (saffron) rhizosphere during its flowering period (october-november). bacterial load was compared between rhizosphere and bulk soil by counting cfu/gm of roots and soil respectively, and was found to be ~40 times more in rhizosphere. in total 100 bacterial isolates were selected randomly from rhizosphere and bulk soil (50 each) and screened for in-vitro and in vivo plant g ...201323749248
[effects of continuous application of bio-organic fertilizer on banana production and cultural microflora of bulk soil in orchard with serious disease incidence].a field experiment was conducted for two years to investigate the effects of different fertilization applications on the suppression of banana fusarium wilt disease, crop yield, fruit quality and culturable microflora in a banana orchard which has been monocultured with banana for 12 years and suffered serious banana fusarium wilt disease. the fertilizers included chemical fertilizer (cf), cow manure compost (cm), pig manure compost (pm) and bio-organic fertilizer (bio). the banana soil microflo ...201526094464
mechanism for the primary transformation of acetaminophen in a soil/water system.the transformation of acetaminophen (apap) in a soil/water system was systematically investigated by a combination of kinetic studies and a quantitative analysis of the reaction intermediates. biotransformation was the predominant pathway for the elimination of apap, whereas hydrolysis or other chemical transformation, and adsorption processes made almost no contribution to the transformation under a dark incubation. bacillus aryabhattai strain 1-sj-5-2-5-m, klebsiella pneumoniae strain s001, an ...201627107139
rethinking the niche of upper-atmosphere bacteria: draft genome sequences of bacillus aryabhattai c765 and bacillus aerophilus c772, isolated from rice fields.here, we report two genome sequences of endospore-forming bacteria isolated from the rice fields of comporta, portugal, identified as bacillus aryabhattai c765 and bacillus aerophilus c772. both species were previously identified in air samples from the upper atmosphere, but our findings suggest their presence in a wider range of environmental niches.201525858824
biodegradation of the cross-linked copolymer of acrylamide and potassium acrylate by soil bacteria.chemical cross-linking and the high molecular weight of superabsorbent copolymers (saps) are the two main causes of their resistance to biodegradation. however, sap particles are colonized by microorganisms. for the purposes of this study, the dry technical copolymer of acrylamide and potassium acrylate containing 5.28 % of unpolymerized monomers was wrapped in a geotextile and incubated in unsterile haplic luvisol soil as a water absorbing geocomposite. the highest number of soil bacteria that ...201626817471
molecular characterization of midgut microbiota of aedes albopictus and aedes aegypti from arunachal pradesh, india.microbiota inhabiting midguts of mosquitoes play a key role in the host - parasite interaction and enhance vectorial capacity of viral diseases like dengue and chikungunya fevers. mosquito midgut is considered to be an important site for host-pathogen interaction and pathogen survival is thought to be an outcome of this interaction. in the present study we examined the bacterial community in the midgut of aedes mosquitoes in arunanchal pradesh, india, a subtropical zone where dengue fever is rep ...201526684012
draft genome sequence of the endophytic bacillus aryabhattai strain squ-r12, identified from phoenix dactylifera l. roots.bacillus aryabhattai strain squ-r12 was isolated from date palm seedlings, where it showed a growth-promoting capacity by being able to synthesize indole-3-acetic acid phytohormone and reduce ethylene biosynthesis by producing 1-aminocyclopropane-1-carboxylic acid deaminase. the draft genome sequence of this strain is reported here.201728798173
isolation, characterization, and use for plant growth promotion under salt stress, of acc deaminase-producing halotolerant bacteria derived from coastal soil.in total, 140 halotolerant bacterial strains were isolated from both the soil of barren fields and the rhizosphere of six naturally growing halophytic plants in the vicinity of the yellow sea, near the city of incheon in the republic of korea. all of these strains were characterized for multiple plant growth promoting traits, such as the production of indole acetic acid (iaa), nitrogen fixation, phosphorus (p) and zinc (zn) solubilization, thiosulfate (s2o3) oxidation, the production of ammonia ...201021124065
biological treatment of model dyes and textile wastewaters.previous works conducted in our laboratory, reveled that bacillus aryabhattai dc100 produce ligninolytic enzymes such as laccases and/or peroxidases, opening new applications in different bioprocesses, including the treatment of disposal residues such as dyestuffs from textile processing industries. this work described the degradation of three commercial model dyes coomassie brilliant blue g-250 (cbb), indigo carmine (ic) and remazol brilliant blue r (rbbr) under different culture media and oper ...201728437742
janibacter hoylei sp. nov., bacillus isronensis sp. nov. and bacillus aryabhattai sp. nov., isolated from cryotubes used for collecting air from the upper atmosphere.three novel bacterial strains, pvas-1(t), b3w22(t) and b8w22(t), were isolated from cryotubes used to collect air samples at altitudes of between 27 and 41 km. based on phenotypic characteristics, chemotaxonomic features, dna-dna hybridization with the nearest phylogenetic neighbours and phylogenetic analysis based on partial 16s rrna gene sequences (pvas-1(t), 1196 nt; b3w22(t), 1541 nt; b8w22(t), 1533 nt), the three strains were identified as representing novel species, and the names proposed ...200919643890
application of antifungal cfb to increase the durability of cement mortar.antifungal cement mortar or microbiological calcium carbonate precipitation on cement surface has been investigated as functional concrete research. however, these research concepts have never been fused with each other. in this study, we introduced the antifungal calciteforming bacteria (cfb) bacillus aryabhattai knuc205, isolated from an urban tunnel (daegu, south korea). the major fungal deteriogens in urban tunnel, cladosporium sphaerospermum knuc253, was used as a sensitive fungal strain. b ...201222580322
diversity and physiology of culturable bacteria associated with a coastal antarctic ice core.microbiological studies of polar ice at different depths may provide important comparisons, as they preserve records of microbial cells and past climate. in this study, we examined bacterial abundance, diversity and glaciochemical composition from three depths of an ice core from coastal dronning maud land, east antarctica. higher bacterial abundance corresponded with high in situ sea-salt na(+) and dust concentration, suggesting that bacteria might have been transported and deposited into ice a ...201222537873
extracellular l-asparaginase from a protease-deficient bacillus aryabhattai itbhu02: purification, biochemical characterization, and evaluation of antineoplastic activity in vitro.an extracellular l-asparaginase produced by a protease-deficient isolate, bacillus aryabhattai itbhu02, was purified to homogeneity using ammonium sulfate fractionation and subsequent column chromatography on diethylaminoethyl-sepharose fast flow and seralose cl-6b. the enzyme was purified 68.9-fold with specific activity of 680.47 u mg(-1). the molecular weight of the purified enzyme was approximately 38.8 kda on sds-page and 155 kda on native page gel as well as gel filtration column revealing ...201323996139
statistical and evolutionary optimization for enhanced production of an antileukemic enzyme, l-asparaginase, in a protease-deficient bacillus aryabhattai itbhu02 isolated from the soil contaminated with hospital waste.over the past few decades, l-asparaginase has emerged as an excellent anti-neoplastic agent. in present study, a new strain itbhu02, isolated from soil site near degrading hospital waste, was investigated for the production of extracellular l-asparaginase. further, it was renamed as bacillus aryabhattai itbhu02 based on its phenotypical features, biochemical characteristics, fatty acid methyl ester (fame) profile and phylogenetic similarity of 16s rdna sequences. the strain was found protease-de ...201324195352
survival of introduced phosphate-solubilizing bacteria (psb) and their impact on microbial community structure during the phytoextraction of cd-contaminated soil.this study was conducted to investigate whether or not phosphate-solubilizing bacteria (psb) as a kind of plant growth promoting rhizobacteria enhance the uptake of cd by plants. in addition, the effect of psb augmentation during phytoextraction on the microbial community of indigenous soil bacteria was also studied. in the initial cd-contaminated soil, the major phyla were proteobacteria (35%), actinobacteria (38%) and firmicutes (8%). while proteobacteria were dominant at the second and sixth ...201324231320
bacillus qingshengii sp. nov., a rock-weathering bacterium isolated from weathered rock surface.a novel type of rock-weathering bacterium was isolated from weathered rock (tuff) surface collected from dongxiang (jiangxi, eastern china). cells of strain g19(t) were gram-reaction-positive, rod-shaped, endospore-forming and non-motile. the strain was aerobic, catalase- and oxidase-positive, and grew optimally at 30 °c and ph 7.0. on the basis of 16s rrna gene sequence analysis, strain g19(t) was shown to belong to the genus bacillus and the closest phylogenetic relatives were bacillus aryabha ...201424801156
draft genome of bagasse-degrading bacteria bacillus aryabhattai gz03 from deep sea water.bacillus aryabhattai gz03 was isolated from deep sea water of the south china sea, which can produce glucose and fructose by degrading bagasse at 25 °c. here we report the draft genome sequence of bacillus aryabhattai gz03. the data obtained revealed 37 contigs with genome size of 5,105,129 bp and g+c content of 38.09%. the draft genome of b. aryabhattai gz03 may provide insights into the mechanism of microbial carbohydrate and lignocellulosic material degradation.201525479947
growth promotion of xanthium italicum by application of rhizobacterial isolates of bacillus aryabhattai in microcosm soil.this study was conducted using rhizobacteria, which are able to exert beneficial effects upon plant growth in the infertile soil collected from barren lakeside areas. four strains of plant growth promoting bacteria were isolated from the rhizosphere of a common wild plant, erigeron canadensis. isolated strains ls9, ls11, ls12, and ls15 were identified as bacillus aryabhattai by 16s rdna sequence analysis. b. aryabhattai ls9, ls11, ls12, and ls15 could solubilize 577.9, 676.8, 623.6, and 581.3 mg ...201222367936
bacterial inoculants for enhanced seed germination of spartina densiflora: implications for restoration of metal polluted areas.the design of effective phytoremediation programs is severely hindered by poor seed germination on metal polluted soils. the possibility that inoculation with plant growth promoting rhizobacteria (pgpr) could help overcoming this problem is hypothesized. our aim was investigating the role of pgpr in spartina densiflora seed germination on sediments with different physicochemical characteristics and metal pollution degrees. gram negative pantoea agglomerans rso6 and rso7, and gram positive bacill ...201627315751
screening beneficial rhizobacteria from spartina maritima for phytoremediation of metal polluted salt marshes: comparison of gram-positive and gram-negative strains.the aim of our work was the isolation and characterization of bacteria from the rhizosphere of spartina maritima in the metal contaminated odiel estuary (huelva, sw spain). from 25 strains, 84 % were identified as gram-positive, particularly staphylococcus and bacillus. gram-negative bacteria were represented by pantoea and salmonella. salt and heavy metal tolerance, metal bioabsorption, plant growth promoting (pgp) properties, and biofilm formation were investigated in the bacterial collection. ...201627417328
bacillus aryabhattai ba03: a novel approach to the production of natural value-added compounds.a strain designated as ba03, with the ability to transform ferulic acid into vanillin and 4-vinylguaiacol, was isolated from contaminated cryovials. the production of natural value-added compounds was dependent on the media employed. the morphological and physiological characteristics of this strain were compared with those of the typical vanillin-producer strain amycolatopsis sp. atcc 39116. according to a partial 16s rrna sequence, we determined that ba03 belonged to bacillus aryabhattai. in a ...201627562593
arsenic mediated modifications in bacillus aryabhattai and their biotechnological applications for arsenic bioremediation.the present study reports the arsenic (as) tolerance mechanism of bacteria bacillus aryabhattai (nbri014). the data explores the intracellular accumulation and volatilization of as from the culture medium after 48 h of exposure to 25,000 mg l(-1) arsenate as(v). the study also provides the evidence of presence of ars operon in bacteria, which may have played an important role in reducing as toxicity. additionally, we found 7 differentially expressed proteins to be up-regulated in bacterial cells ...201627619063
calcite dissolution by brevibacterium sp. soti06: a futuristic approach for the reclamation of calcareous sodic soils.assessing the ability of soil microorganisms to dissolute poorly soluble native calcite to supply ca(2+) is a new area to be explored in reclaiming sodic soils by supplying adequate ca(2+) and reducing the recurrent sodicity. hence, the present study aimed to isolate a calcite dissolving bacteria (cdb) from calcareous sodic soils and to understand the mechanism of calcite dissolution. of the 33 cdb isolates recovered from the calcareous sodic soils of tamil nadu (coimbatore, ramnad, and trichy), ...201628008333
a novel extracellular low-temperature active phytase from bacillus aryabhattai rs1 with potential application in plant growth.bacillus aryabhattai rs1 isolated from rhizosphere produced an extracellular, low temperature active phytase. the cultural conditions for enzyme production were optimized to obtain 35 u ml(-1) of activity. purified phytase had specific activity and molecular weight of 72.97 u mg(-1) and ∼40 kda, respectively. the enzyme was optimally active at ph 6.5 and 40°c and was highly specific to phytate. it exhibited higher catalytic activity at low temperature, retaining over 40% activity at 10°c. phytas ...201728248011
bacillus aryabhattai srb02 tolerates oxidative and nitrosative stress and promotes the growth of soybean by modulating the production of phytohormones.plant growth promoting rhizobacteria (pgpr) are diverse, naturally occurring bacteria that establish a close association with plant roots and promote the growth and immunity of plants. established mechanisms involved in pgpr-mediated plant growth promotion include regulation of phytohormones, improved nutrient availability, and antagonistic effects on plant pathogens. in this study, we isolated a bacterium from the rhizospheric soil of a soybean field in chungcheong buk-do, south korea. using 16 ...201728282395
evaluation of short-chain-length polyhydroxyalkanoate accumulation in bacillus aryabhattai.this study was focused on the polyhydroxybutyrate (phb) accumulation property of bacillus aryabhattai isolated from environment. twenty-four polyhydroxyalkanoate (pha) producers were screened out from sixty-two environmental bacterial isolates based on sudan black b colony staining. based on their pha accumulation property, six promising isolates were further screened out. the most productive isolate phb10 was identified as b. aryabhattai phb10. the polymer production maxima were 3.264g/l, 2.181 ...201728359856
genome-guided insights into the plant growth promotion capabilities of the physiologically versatile bacillus aryabhattai strain ab211.bacillus aryabhattai ab211 is a plant growth promoting, gram-positive firmicute, isolated from the rhizosphere of tea (camellia sinensis), one of the oldest perennial crops and a major non-alcoholic beverage widely consumed all over the world. the whole genome of b. aryabhattai ab211 was sequenced, annotated and evaluated with special focus on genomic elements related to plant microbe interaction. it's genome sequence reveals the presence of a 5,403,026 bp chromosome. a total of 5226 putative pr ...201728377746
biodiversity of aerobic endospore-forming bacterial species occurring in yanyanku and ikpiru, fermented seeds of hibiscus sabdariffa used to produce food condiments in benin.yanyanku and ikpiru made by the fermentation of malcavene bean (hibiscus sabdariffa) are used as functional additives for parkia biglobosa seed fermentations in benin. a total of 355 aerobic endospore-forming bacteria (aefb) isolated from yanyanku and ikpiru produced in northern and southern benin were identified using phenotypic and genotypic methods, including gtg5-pcr, m13-pcr, 16s rrna, gyra and gyrb gene sequencing. generally, the same 5-6 species of the genus bacillus predominated: bacillu ...201323571124
application of magnetic oms-2 in sequencing batch reactor for treating dye wastewater as a modulator of microbial community.the potential and mechanism of synthesized magnetic octahedral molecular sieve (fe3o4@oms-2) nanoparticles in enhancing the aerobic microbial ability of sequencing batch reactor (sbr) for treating dye wastewater have been revealed in this study. the addition of fe3o4@oms-2 of 0.25g/l enhanced the decolorization of sbrs with an operation cycle of 24h by more than 20%. the 16s rrna gene high-throughput sequencing indicated fe3o4@oms-2 increased the microbial richness and diversity of sbrs, and mor ...201728711831
fed-batch production of vanillin by bacillus aryabhattai ba03.bacillus aryabhattai ba03, a strain isolated in our laboratory, has interesting properties related to the production of natural aromas and flavors. specifically, we have found that it was able to produce vanillin from ferulic acid (fa). furthermore, this strain produces high amounts of 4-vinylguaiacol in only 14h, this being the only intermediate metabolite observed in the process. fa is an inexpensive feedstock for the production of natural value-added compounds when extracted from lignocellulo ...201728803126
bacillus zanthoxyli sp. nov., a novel nematicidal bacterium isolated from chinese red pepper (zanthoxylum bungeanum maxim) leaves in china.a novel strain, 1433(t), was isolated from leaves of chinese red pepper (huajiao, zanthoxylum bungeanum maxim) collected from gansu province in northwestern china, and was characterised by a polyphasic approach. cells of strain 1433(t) were observed to be gram-stain positive, aerobic, asporogenous, rod shaped, motile and to have peritrichous flagella. the strain was observed to grow at a range of temperatures and ph, 4-45 °c (optimum 28-32 °c) and 6.0-10.0 (optimum ph 6.0-7.0), respectively. gro ...201728550345
zinc solubilizing bacillus spp. potential candidates for biofortification in maize.bioaugmentation of zn solubilizing rhizobacteria could be a sustainable intervention to increase bioavailability of zn in soil which can be helpful in mitigation of yield loss and malnutrition of zinc. in present study, a number of pure rhizobacterial colonies were isolated from maize rhizosphere and screened for their ability to solubilize zinc oxide. these isolates were screened on the basis of zinc and phosphate solubilization, iaa production, protease production, catalase activity and starch ...201728647123
[microecological mechanisms of red-leaf disease occurrence in salvia miltiorrhiza bge].a comparative study was made on the nutrient content in rhizosphere soil and the microflora in rhizosphere soil and on rhizoplane of healthy and red-leaf diseased salvia miltiorrhiza plants, aimed to approach the microecological mechanisms of red-leaf disease occurrence in s. miltiorrhiza. the n, p, k, and mn contents in the diseased plant leaves were significantly lower than those in the healthy plant leaves (p < 0.05). no significant difference was observed in the available p content in the rh ...201324175532
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