| 'candidatus midichloria mitochondrii', an endosymbiont of the tick ixodes ricinus with a unique intramitochondrial lifestyle. | an intracellular bacterium with the unique ability to enter mitochondria exists in the european vector of lyme disease, the hard tick ixodes ricinus. previous phylogenetic analyses based on 16s rrna gene sequences suggested that the bacterium formed a divergent lineage within the rickettsiales (alphaproteobacteria). here, we present additional phylogenetic evidence, based on the gyrb gene sequence, that confirms the phylogenetic position of the bacterium. based on these data, as well as electron ... | 2006 | 17082386 |
| detection of alpha and gamma-proteobacteria in amblyomma triste (acari: ixodidae) from uruguay. | amblyomma triste is the most prevalent tick species reported in human tick bites in uruguay and has been found to be infected with rickettsia parkeri, but no other microorganisms have been reported from this tick. a sample of 254 adults of a. triste was collected by flagging on vegetation in suburban areas in southern uruguay. pools of five ticks were assembled and a screening for the dna from the resulting 51 pools was realized by pcr assays using primers for amplifying a fragment of 16s rrna g ... | 2008 | 18071910 |
| midichloria mitochondrii is widespread in hard ticks (ixodidae) and resides in the mitochondria of phylogenetically diverse species. | the hard tick ixodes ricinus (ixodidae) is the sole animal thus far shown to harbour an intra-mitochondrial bacterium, which has recently been named midichloria mitochondrii. the objectives of this work were (i) to screen ixodid ticks for midichloria-related bacteria and (ii) to determine whether these bacteria exploit the intra-mitochondrial niche in other tick species. our main goal was to discover further models of this peculiar form of symbiosis. we have thus performed a pcr screening for mi ... | 2008 | 18205982 |
| diversity of ixodes ricinus tick-associated bacterial communities from different forests. | nymphal ixodes ricinus ticks (n=180) were collected from three different areas in the netherlands to investigate the effect of forest composition on tick-associated microbial communities. sampled habitats differed in thickness of leaf litter and humus layers and vegetation associations and were located near amsterdam (beech-oak), ede (birch-oak) and veldhoven (birch-oak). analysis of nine 16s rrna gene clone libraries made from individual ticks showed nearest matches with presumed pathogens cand ... | 2008 | 18355299 |
| molecular identification of anaplasma marginale and rickettsial endosymbionts in blood-sucking flies (diptera: tabanidae, muscidae) and hard ticks (acari: ixodidae). | in an attempt to identify the main vector and possible transmission routes of anaplasma spp. in a region of hungary with high prevalence of ovine and bovine anaplasmosis, dna was extracted from 316 haematophagous arthropods (individually or in pools), including 4 species of ixodid ticks, 6 species of tabanid flies and hornflies. midichloria-like organisms were identified with pcr (amplifying a portion of the 16s rrna gene) and sequencing from dermacentor marginatus and ixodes ricinus. significan ... | 2008 | 18495345 |
| "candidatus midichloria" endosymbionts bloom after the blood meal of the host, the hard tick ixodes ricinus. | "candidatus midichloria mitochondrii," an intracellular symbiont of the tick ixodes ricinus, is the only described organism able to invade the mitochondria of any multicellular organism. we used quantitative pcr to examine cycles of bacterial growth and death throughout the host's development and found that they correspond with the phases of engorgement and molt, respectively. | 2008 | 18689508 |
| incidence of various tick-borne microorganisms in rodents and ticks of central slovakia. | in this study, we detected rickettsia helvetica, candidatus midichloria mitochondrii, anaplasma phagocytophilum, ehrlichia muris, candidatus neoehrlichia mikurensis, and bartonella sp. infections in wild rodents and ticks collected from the vegetation of central slovakia. the microorganisms were identified by pcr and sequencing. yellow-necked mice (apodemus flavicollis) were infected with e. muris and bartonella sp., while ticks ixodes ricinus collected from the vegetation were infected with r. ... | 2008 | 18999892 |
| detection of a new bacterium related to 'candidatus midichloria mitochondrii' in bed bugs. | | 2009 | 19438654 |
| absence of the symbiont candidatus midichloria mitochondrii in the mitochondria of the tick ixodes holocyclus. | candidatus midichloria mitochondrii (m. mitochondrii) belongs to a novel clade of bacteria within the order rickettsiales. recent pcr-based screening studies indicate that it is present in a number of blood-sucking arthropods, as well as the blood of some vertebrates. its medical and veterinary significance remains to be determined. electron microscopic examinations of m. mitochondrii have thus far been conducted on two infected tick species. remarkably, the bacterium was found in abundance with ... | 2009 | 19732154 |
| "candidatus anadelfobacter veles" and "candidatus cyrtobacter comes," two new rickettsiales species hosted by the protist ciliate euplotes harpa (ciliophora, spirotrichea). | the order rickettsiales (alphaproteobacteria) is a well-known group containing obligate endocellular prokaryotes. the order encompasses three families (rickettsiaceae, anaplasmataceae, and holosporaceae) and a fourth, family-level cluster, which includes only one candidate species, "candidatus midichloria mitochondrii," as well as several unnamed bacterial symbionts. the broad host range exhibited by the members of the "candidatus midichloria" clade suggests their eventual relevance for a better ... | 2010 | 20435776 |
| molecular detection of ehrlichia canis, anaplasma bovis, anaplasma platys, candidatus midichloria mitochondrii and babesia canis vogeli in ticks from israel. | : ticks are vectors of important pathogens of human and animals. therefore, their microbial carriage capacity is constantly being investigated. the aim of this study was to characterize the diversity of domestic animal pathogens in ticks collected from vegetation and the ground, from different parts of israel. non-engorged questing adult ticks were collected from 13 localities. a total of 1196 ticks in 131 pools-83 pools of rhipicephalus turanicus and 48 of rhipicephalus sanguineus (with two to ... | 2011 | 20636417 |
| novel genotypes of anaplasma bovis, "candidatus midichloria" sp. and ignatzschineria sp. in the rocky mountain wood tick, dermacentor andersoni. | bovine anaplasmosis, caused by anaplasma marginale, is a vector-borne disease that is enzootic in many parts of the usa. although dermacentor andersoni, a major vector of a. marginale, occurs in canada, the canadian cattle herds are currently considered free of bovine anaplasmosis. there have been two outbreaks of the disease in the province of saskatchewan, but these have been linked to the importation of infected animals. however, the distribution of bovine anaplasmosis may alter with range ex ... | 2011 | 21334146 |
| absence of zoonotic bartonella species in questing ticks: first detection of bartonella clarridgeiae and rickettsia felis in cat fleas in the netherlands. | abstract: | 2011 | 21501464 |
| detection of bacteria related to candidatus midichloria mitochondrii in tick cell lines. | many ticks have been shown to be infected with intracellular bacteria. one of these bacteria is candidatus midichloria mitochondrii which is the only characterized bacterium that has the ability to invade the mitochondria within ovarian cells and consume them without any effect on the female tick's reproduction. in the present study, eight cell lines derived from the ticks ixodes ricinus, ixodes scapularis, rhipicephalus (boophilus) microplus, and rhipicephalus (boophilus) decoloratus were exami ... | 2011 | 21748354 |
| phylogenomic evidence for the presence of a flagellum and cbb3 oxidase in the free-living mitochondrial ancestor. | the initiation of the intracellular symbiosis that would give rise to mitochondria and eukaryotes was a major event in the history of life on earth. hypotheses to explain eukaryogenesis fall into two broad and competing categories: those proposing that the host was a phagocytotic proto-eukaryote that preyed upon the free-living mitochondrial ancestor (hereafter fma) and those proposing that the host was an archaebacterium that engaged in syntrophy with the fma. of key importance to these hypothe ... | 2011 | 21690562 |