| conventional and pcr detection of aphelenchoides fragariae in diverse ornamental host plant species. | a pcr-based diagnostic assay was developed for early detection and identification of aphelenchoides fragariae directly in host plant tissues using the species-specific primers afragfl and afragrl that amplify a 169-bp fragment in the internal transcribed spacer (its1) region of ribosomal dna. these species-specific primers did not amplify dna from aphelenchoides besseyi or aphelenchoides ritzemabosi. the pcr assay was sensitive, detecting a single nematode in a background of plant tissue extract ... | 2007 | 19259510 |
| seasonal fluctuations of soil and tissue populations of ditylenchus dipsaci and aphelenchoides ritzemabosi in alfalfa. | population dynamics of a. ritzemabosi and d. dipsaci were studied in two alfalfa fields in wyoming. symptomatic stem-bud tissue and root-zone soil from alfalfa plants exhibiting symptoms of d. dipsaci infection were collected at intervals of 3 to 4 weeks. both nematodes were extracted from stem tissue with the baermann funnel method and from soil with the sieving and baermann funnel method. soil moisture and soil temperature at 5 cm accounted for 64.8% and 61.0%, respectively, of the variability ... | 1999 | 19270872 |
| distribution in the western united states on alfalfa and cultivar reaction to mixed populations of ditylenchus dipsaci and aphelenchoides ritzemabosi. | ditylenchus dipsaci and aphelenchoides ritzemabosi were extracted from 29 of 40 plant samples (72.5%) collected from arizona, california, colorado, idaho, montana, oregon, south dakota, utah, washington, and wyoming. percentages of a. ritzemabosi in tissue of the 29 samples ranged from 1.77 to 67.82%. only ditylenchus dipsaci was recovered from the remaining 11 samples. all of the 16 fields sampled in wyoming contained both nematodes. percentages of a. ritzemabosi in the wyoming samples ranged f ... | 1994 | 19279952 |
| anhydrobiosis in five species of plant associated nematodes. | five species of nematodes - hemicriconemoides pseudobrachyurum, hemicycliophora conida, macroposthonia ornata, aphelenchoides ritzemabosi, and psilenchus hilarulus -were desiccated to study their capacity to survive anhydrobiotically. results indicate that the ability of the sheath to shrink quickly and its relatively loose attachment with the nematode body allow h. conida to survive longer than h. pseudobrachyurum; the survival of m. ornata was intermediate, a. ritzemabosi and p. hilarulus surv ... | 1984 | 19295887 |
| the influence of environmental factors on the respiration of plant-parasitic nematodes. | respiration of selected nematode species was measured relative to co level, temperature, osmotic pressure, humidity, glucose utilization and high ionic concentrations of sodium and potassium.in general, respiration was stimulated most by the dominant environmental factors at levels near those expected in the nematode's "natural" habitat. soil-inhabiting nematodes utilized o, most rapidly with high (1-2%) co whereas a foliar nematode (aphelenchoides ritzemabosi) did so with 0.03% co, the concentr ... | 1970 | 19322313 |
| arabidopsis thaliana as a suitable model host for research on interactions between plant and foliar nematodes, parasites of plant shoot. | the rice white tip nematode (rwtn), aphelenchoides besseyi and the chrysanthemum foliar nematode (cfn), aphelenchoides ritzemabosi are migratory plant parasitic nematodes that infect the aboveground parts of plants. in this research, arabidopsis thaliana was infected by rwtn and cfn under indoor aseptic cultivation, and the nematodes caused recognizable symptoms in the leaves. furthermore, rwtn and cfn completed their life cycles and proliferated. therefore, a. thaliana was identified as a new h ... | 2016 | 27910895 |
| transcriptome analysis of the chrysanthemum foliar nematode, aphelenchoides ritzemabosi (aphelenchida: aphelenchoididae). | the chrysanthemum foliar nematode (cfn), aphelenchoides ritzemabosi, is a plant parasitic nematode that attacks many plants. in this study, a transcriptomes of mixed-stage population of cfn was sequenced on the illumina hiseq 2000 platform. 68.10 million illumina high quality paired end reads were obtained which generated 26,817 transcripts with a mean length of 1,032 bp and an n50 of 1,672 bp, of which 16,467 transcripts were annotated against six databases. in total, 20,311 coding region seque ... | 2016 | 27875578 |
| the effect of two fixation methods (taf and dess) on morphometric parameters of aphelenchoides ritzemabosi. | identification of nematode species by using conventional methods requires fixation of the isolated material and a suitable preparation for further analyses. tentative identification using microscopic methods should also be performed prior to initiating molecular studies. in the literature, various methods are described for the preparation of nematodes from the genus aphelenchoides for identification and microscopic studies. the most commonly used fixatives are formalin (timm 1969; szczygieł & ci ... | 2016 | 27394233 |