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maritimibacter alkaliphilus gen. nov., sp. nov., a genome-sequenced marine bacterium of the roseobacter clade in the order rhodobacterales.a gram-negative, chemoheterotrophic, strictly aerobic, alkaliphilic, rod-shaped marine bacterium, designated htcc2654(t), was isolated from the western sargasso sea by using a dilution-to-extinction culturing method. phylogenetic analyses based on 16s rrna gene sequences showed that strain htcc2654(t) belonged to the roseobacter clade of the order rhodobacterales. the 16s rrna gene sequence similarity of the strain with respect to other members of the roseobacter clade ranged from 90.4 to 95.1 % ...200717625211
lutimaribacter saemankumensis gen. nov., sp. nov., isolated from a tidal flat of the yellow sea.a gram-negative, non-motile, rod-shaped bacterial strain, designated smk-117t, belonging to the alphaproteobacteria, was isolated from a tidal flat of the yellow sea, korea, and was subjected to a polyphasic taxonomic study. strain smk-117t grew optimally at ph 7.0-8.0 and 30 degrees c in the presence of 2 % (w/v) nacl. neighbour-joining and maximum-likelihood phylogenetic trees based on 16s rrna gene sequences showed that strain smk-117t clustered with maritimibacter alkaliphilus htcc2654t, wit ...200919126722
hwanghaeicola aestuarii gen. nov., sp. nov., a moderately halophilic bacterium isolated from a tidal flat of the yellow sea.a moderately halophilic gram-staining-negative bacterium, designated strain y26(t), was isolated from a tidal flat of taean coast in south korea. cells were strictly aerobic, motile cocci with a single flagellum and showed catalase- and oxidase-positive reactions. growth of strain y26(t) was observed at 15-35 °c (optimum 25-30°c), ph 6.0-8.0 (optimum ph6.5-7.5) and with 1.5-6.0 % (w/v) nacl (optimum 2.0-3.0 %). the predominant fatty acids were c(18 : 1)ω7c (66.2 %), c(16 : 0) (12.4 %) and ...201020081009
biocatalytic resolution of glycidyl phenyl ether using a novel epoxide hydrolase from a marine bacterium, maritimibacter alkaliphilus kccm 42376 [corrected].as a continuous effort of developing highly enantioselective epoxide hydrolase from marine microorganisms, it was found that maritimibacter alkaliphilus kccm 42376 [corrected] was highly enantioselective toward racemic glycidyl phenyl ether (gpe). an open reading frame (orf) encoding a putative epoxide hydrolase (ehase) was cloned from the genome of maritimibacter alkaliphilus kccm 42376 [corrected], followed by expression and purification in escherichia coli. the purified ehase (reh) hydrolyzed ...201020471590
jhaorihella thermophila gen. nov., sp. nov., a new moderately thermophilic bacterium isolated from a coastal hot spring.a beige-coloured, gram negative, aerobic, non-motile moderately thermophilic, rod-shaped bacterium, cc-mhsw-1t, was isolated from a coastal hot spring of green island (lutao), located off taituang, taiwan, on marine agar 2216. the 16s rrna gene sequence analysis demonstrated that it shared <94% sequence similarity with lutimaribacter, maritimibacter, and oceanicola. ubiquinone (q-10) was the major respiratory quinone and c18:1omega7c was the predominant fatty acid. the predominant polar lipids c ...201020656808
genome sequences of pelagibaca bermudensis htcc2601t and maritimibacter alkaliphilus htcc2654t, the type strains of two marine roseobacter genera.pelagibaca bermudensis htcc2601(t) and maritimibacter alkaliphilus htcc2654(t) represent two marine genera in the globally significant roseobacter clade of the alphaproteobacteria. here, we present the genome sequences of these organisms, isolated from the sargasso sea using dilution-to-extinction culturing, which offer insight into the genetic basis for the metabolic and ecological diversity of this important group.201020729358
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