occurrence of a blood group a-like substance in eggs of the prosobranch snail pomacea the eggs of the prosobranch snails pomacea canaliculata and pomacea insularum a blood group a-like substance has been detected by anti-a from the snails helix pomatia, helix aspersa and cepaea nemoralis.1976815102
catadiscus pomaceae sp. n. (trematoda, paramphistomatidae) from pomacea canaliculata (lamarck, 1801) (prosobranchia, ampullariidae).catadiscus pomaceae sp. n. from the intestine of the prosobranch mollusc pomacea canaliculata (lamarck, 1801), is described. the host snail was collected from a lenitic biotope belonging to the riachuelo basin (corrientes province, argentina) during 1985-1986. so far the species of the genus catadiscus cohn, 1904 have been recorded in amphibians and reptiles. this is the first instance of a species of that genus parasitizing a mollusc.19921308560
predation of pomacea canaliculata (ampullariidae) on adult biomphalaria peregrina (planorbidae). 19902331180
hemocytes of pomacea canaliculata: i. reversible aggregation induced by ca2+.using a platelet aggregometer, factors involved in hemocyte aggregation of a prosobranch snail pomacea canaliculata were investigated. aggregation-inducing tests revealed that extracellular ca2+ is required to provoke the cellular response which is reversible. the aggregation-dispersion response can be induced by adding ca2+ and edta alternately. aggregation was inhibited by a calmodulin antagonist n-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (w-7) in a dose-dependent manner, and by a prot ...19902369966
phylogenetic study of the oxytocin-like immunoreactive system in invertebrates.1. a phylogenetic study of oxytocin (oxt)-like immunoreactive cells was performed by the pap method in the central nervous system of invertebrates. 2. the immunoreactivity was detected in the nerve cells of hydra magnipapillata of the coelenterata; neanthes japonica and pheretima communissima of the annelida; oncidium verrucosum, limax marginatus and meretrix lamarckii of the mollusca; and baratha brassica of the arthropoda. 3. no immunoreactive cells were found in bipalium sp. of the platyhelmi ...19882907439
phylogenetic study of the arginine-vasotocin/arginine-vasopressin-like immunoreactive system in invertebrates.1. a phylogenetic study of arg-vasotocin (avt)/arg-vasopressin (avp)-like immunoreactive cells was performed by the pap method in the central nervous system of invertebrates. 2. the immunoreactivity was detected in the nerve cells of hydra magnipapillata of the coelenterata; neanthes japonica and pheretima communissima of the annelida; pomacea canaliculata, aplysia kurodai, oncidium verrucosum, bradybaena similaris, achatina fulica, limax marginatus and meretrix lamarckii of the mollusca; gnorim ...19882907440
the inhibition of trypsin and some other proteases by ovorubin, a protein from the eggs of pomacea canaliculata. 19724263076
sex ratio and susceptibility of the golden apple snail, pomacea canaliculata.golden apple snails, pomacea canaliculata, were collected at two localities having different ecological environments. in both canal and pond, p. canaliculata males were found more than females during the dry season (summer and winter). in the canal, the male snails were highest in number (86.67%) in may. when rain started, they began decreasing and were lowest at 33.33% in august. of 575 snails collected, 30.6% were infected by one or more of the three groups of amphistome, distome and echinosto ...19947855663
natural parasitic infection of the golden apple snail, pomacea canaliculata.golden apple snails, pomacea canaliculata, were collected once a month during a year to search for their natural parasites. the collections were made at two localities having different ecological environments. of 576 collected snails from a canal, 176 individuals (30.6%) were infected by three groups of metacercariae. these parasites were amphistome, distome and echinostome metacercariae, which had prevalences of 23.5, 19.5 and 0.5%, respectively. the incidence of infection was highest (68.4% in ...19938362292
purification and characterization of an alpha-l-fucosidase from pomacea alpha-l-fucosidase (ec was isolated from the hepatopancreas of pomacea canaliculata. the enzyme was purified 285-fold from the crude enzyme extract by procedures involving first heat treatment, ammonium sulfate fractionation, second heat treatment, and chromatography on deae-sepharose, hydroxylapatite, and l-fucosylamine-ch-sepharose. when assayed by using p-nitrophenyl glycosides as substrates, the final preparation was free from other glycosidase activities and gave a single prote ...19938470892
lipoproteins of the egg perivitelline fluid of pomacea canaliculata snails (mollusca: gastropoda).the lipid and protein composition of the perivitelline fluid of the eggs of pomacea canaliculata was investigated. two lipoproteins (pv 1 and pv 2) and one lipoprotein fraction (pv 3) were detected for the first time in gastropods. they represent 57.0, 7.5, and 35.5% of the egg total proteins, respectively. pv 1 is a glyco-carotene-protein complex with characteristics of a very high-density lipoprotein (vhdl). it has 0.33% lipids, mainly free sterols and phospholipids. the particle has a mw of 3 ...19968972583
purification and some properties of a beta-xylosidase and an alpha-fucosidase from apple snails (pomacea canaliculata).beta-xylosidase and alpha-fucosidase were purified from the viscera of apple snails (pomacea canaliculata) using ammonium sulfate precipitation, hydrophobic chromatography on butyl-toyopearl, gel filtration on sephacryl s-300, and q-sepharose column chromatography. beta-xylosidase and alpha-fucosidase are glycoproteins and their molecular masses were estimated to be approximately 85 kda and 54 kda by sds-polyacrylamide gel electrophoresis, and assumed to be about 96 kda and 230 kda from their se ...19969063971
properties of alpha-mannosidase partially purified from the apple snail, pomacea canaliculata.pomacea canaliculata alpha-mannosidase (260 kda), composed of at least two isoforms with different pi, was partially purified. the activity was maximum at ph 4 and unaltered after incubation at 60 degrees c for 60 min. zncl2, cacl2, nacl, and sh-reagents increased the activity, while mncl2 and edta inhibited it. the enzyme catalyzed the hydrolysis of alpha 1-2, alpha 1-3, and alpha 1-6 mannosidic linkages.19989972247
development of a penis from the vestigial penis in the female apple snail, pomacea canaliculata. 200011147712
permeability barrier in the mantle epithelium lining the testis in the apple-snail pomacea canaliculata (gastropoda: ampullariidae).intercellular junctions are studied in the epithelium lining the testis of the freshwater snail pomacea canaliculata by conventional staining and lanthanum tracer techniques. the junctional complex consists of belt desmosomes and septate junctions. septate junctions are of the pleated-sheet type and they are constantly associated with mitochondria. gap and tight junctions appear to be absent. these septate junctions seem to be the structural correlate of an epithelial permeability barrier that s ...200111392667
freshwater snails and schistosomiasis mansoni in the state of rio de janeiro, brazil: i-- metropolitan order to elaborate a planorbid chart of the state of rio de janeiro a survey of freshwater gastropods in the metropolitan mesoregion of this state was performed and revealed the occurrence of 20 species: antillorbis nordestensis (lucena, 1954); biomphalaria glabrata (say, 1818); biomphalaria schrammi (crosse, 1864); biomphalaria straminea (dunker, 1848); biomphalaria tenagophila (orbigny, 1835); burnupia sp.; drepanotrema anatinum (orbigny, 1835); drepanotrema cimex (moricand, 1839); drepanot ...200111586447
synthesis, distribution, and levels of an egg lipoprotein from the apple snail pomacea canaliculata (mollusca: gastropoda).the site of synthesis of mollusc lipoproteins is hitherto unknown and was investigated for perivitellin 2 (pv2), an egg lipoprotein found in the freshwater snail pomacea canaliculata. tissues (albumen gland, gonad-digestive gland complex, and muscle) from vitellogenic females were incubated in vitro with 14c-leucine at 25 degrees c for 12 hr. at the end of incubation, soluble proteins from tissue homogenates and medium were analyzed for de novo protein synthesis by electrophoresis and hplc, and ...200211857466
pigmented corpuscles in the midgut gland of pomacea canaliculata and other neotropical apple-snails (prosobranchia, ampullariidae): a possible symbiotic association. 200212058376
lipoproteins from plasma and perivelline fluid of the apple snail pomacea canaliculata. 200212058377
old species and new concepts in the taxonomy of pomacea (gastropoda: ampullariidae).the taxonomic history of the south american genus pomacea perry, 1810, and some shifts of systematic concepts during recent decades are briefly reviewed. too many pre-evolutionist, shell-defined species created a gibberish, the only acceptable solution of which being perhaps a conventional, somewhat authoritarian decision based on expertise. the addition of other sources of morphological, biochemical, ecological or genetic information should not solve the problem if it is not accompanied by a so ...200212058383
pomacea canaliculata (gastropoda: ampullariidae): life-history traits and their plasticity. 200212058384
oviductal structure and provision of egg envelops in the apple snail pomacea canaliculata (gastropoda, prosobranchia, ampullariidae). 200212058385
influence of age and body size on alarm responses in a freshwater snail pomacea canaliculata.a hypothesis that size selection of prey by predators elicits size-specific responses from prey was examined. freshwater snails, pomacea canaliculata, ages 1, 3, 7, 15, 30, or 60 days, were given an extract of 3-day-old snails, and 3-day-old snails were given extracts of snails of the otherages oreggs. snails 15 days or younger crawled out of the water in response to the 3-day-old snail extract, but older ones did not. the 3-day-old snails responded to the extracts of snails of all examined ages ...200212474897
a snail with unbiased population sex ratios but highly biased brood sex ratios.extraordinary sex ratio patterns and the underlying sex-determining mechanisms in various organisms are worth investigating, particularly because they shed light on adaptive sex-ratio adjustment. here, we report an extremely large variation in the brood sex ratio in the freshwater snail, pomacea canaliculata. in eight rearing series originating from three wild populations, sex ratios were highly variable among broods, ranging continuously from almost exclusively males to almost exclusively femal ...200312614578
metabolism of ovorubin, the major egg lipoprotein from the apple snail.the site of synthesis of molluscs lipoproteins is little known and was investigated for the egg lipoprotein perivitellin 1 (pv 1) or ovorubin in the freshwater snail pomacea canaliculata. tissues (albumen gland, gonad-digestive gland complex and muscle) of vitellogenic females were incubated in vitro at 25 degrees c for 12 h with 14c leucine. after that, soluble proteins from tissue homogenates and medium samples were analysed for de novo protein synthesis by electrophoresis and hplc, and radiol ...200312619883
molluscicidal saponins from sapindus mukorossi, inhibitory agents of golden apple snails, pomacea canaliculata.extracts of soapnut, sapindus mukorossi gaertn. (sapindaceae) showed molluscicidal effects against the golden apple snail, pomacea canaliculata lamarck. (ampullariidae) with lc(50) values of 85, 22, and 17 ppm after treating 24, 48, and 72 h, respectively. bioassay-directed fractionation of s. mukorossi resulted in the isolation of one new hederagenin-based acetylated saponin, hederagenin 3-o-(2,4-o-di-acetyl-alpha-l-arabinopyranoside)-(1-->3)-alpha-l-rhamnopyranosyl-(1-->2)-alpha-l-arabinopyran ...200312903945
a chromoprotein from the eggs of pomacea canaliculata. 195413230031
ovorubin, a chromoprotein from the eggs of the gastropod mollusc pomacea canaliculata. 195813623805
antioxidant defense system in the apple snail eggs, the role of ovorubin.a novel role of ovorubin as a protection system against oxidative damage in eggs from pomacea canaliculata was investigated. carotenoid composition, and their antioxidant capacity, as well as the carotenoid-apoprotein interaction, were studied for this lipoglycocarotenoprotein. carotenoid extracts from ovorubin were analysed by tlc and spectrophotometry. the major carotenoid was astaxanthin in its free (40%), monoester (24%), and diester (35%) forms, mainly esterified with 16:0 fatty acid. the a ...200414725852
characterization of the major egg glycolipoproteins from the perivitellin fluid of the apple snail pomacea canaliculata.ovorubin and pv2 are the major lipoglycocarotenoproteins present in the perivitellus of the freshwater snail eggs of pomacea canaliculata, a rapidly expanding rice field pest. we have previously characterized these two particles regarding their lipid and protein compositions, their synthesis and tissular distribution, and their contributions of energy and structural precursors for the developing embryo. in the present study, we have characterized the glycosidic moieties associated to these periv ...200415112330
intrahost distribution and transmission of a new species of cyclopoid copepod endosymbiotic to a freshwater snail, pomacea canaliculata (caenogastropoda, ampullariidae), from argentina.a new species of cyclopoid copepod, ozmana huarpium, is described as a symbiont to pomacea canaliculata (lamarck 1822) (caenogastropoda, ampullariidae). rather large numbers (about one hundred copepods per snail) were found, although there was no evidence of harm to the host. to our knowledge, o. haemophila (symbiont to p. maculata), and the currently described species, o. huarpium, are the only copepod species ever recorded as endosymbionts to freshwater invertebrates. while o. haemophila is re ...200415462567
genetic diversity and molecular markers in introduced and thai native apple snails (pomacea and pila).the genetic diversity and species-diagnostic markers in the introduced apple snail, pomacea canaliculata and in the native thai apple snails; pila ampullacea, p. angelica, p. pesmei, and p. polita, were investigated by restriction analysis of coi and are reported for the first time. twenty-one composite haplotypes showing non-overlapping distributions among species were found. genetic heterogeneity analysis indicated significant differences between species (p < 0.0001) and within p. pesmei (p < ...200415469739
freshwater mollusks of medical importance in kalasin province, northeast thailand.a snail survey was performed in six districts around irrigation areas of lampao dam, in kalasin province. the survey caught a total of 5,479 live snails and classed them into five families, 12 genera and 15 species, of which 7 species are suspected of transmitting human parasitic diseases. the seven species were pila polita, pomacea canaliculata, filopaludina (s.) m. martensi, bithynia (digoniostoma) siamensis goniomphalos, melanoides tuberculata, radix rubiginosa, and indoplanorbis exustus. of ...200516124433
genetics of sex-ratio variation inferred from parent-offspring regressions and sib correlations in the apple snail pomacea canaliculata.the brood sex ratio in the apple snail pomacea canaliculata varies almost continuously from all male to all female, but the population sex ratio is nearly 1:1. in this study, regressions of the offspring sex ratio on the sex ratios of the parents' siblings as well as correlations in the brood sex ratios between sisters or brothers were investigated, in order to infer the genetic system that produces the sex-ratio pattern. there were significant positive relationships between the offspring sex ra ...200616189539
pallial oviduct of pomacea canaliculata (gastropoda): ultrastructural studies of the parenchymal cellular types involved in the metabolism of perivitellins.seasonal variations in the morphology of the parenchymal mass and function of the albumen gland/capsule gland complex have been studied in pomacea canaliculata, together with the cellular types involved in the synthesis and secretion of perivitellin fluid components. the two major parenchymal cell types, albumen secretory cells (as) and labyrinthic cells (lc), undergo seasonal variations throughout the annual reproductive cycle, which is divided into three periods. both cellular types show maxim ...200616453107
[studies on susceptibility of pomacea canaliculata of different developmental stages to infection with angiostrongylus cantonensis].objective to study the susceptibility of pomacea canaliculata of different developmental stages to angiostrongylus cantonensis infection.200516562474
the effect of temperature on the development of angiostrongylus cantonensis (chen 1935) in pomacea canaliculata (lamarck 1822).angiostrongyliasis cantonensis, clinically presented as eosinophilic meningitis, is a snail-borne parasitic disease. we studied the effects of different temperatures on the larval development of angiostrongylus cantonensis in the freshwater snail pomacea canaliculata. six groups of snails were infected and each group was cultured under different temperature conditions. at predefined intervals, four snails from each group were dissected to examine the larval development. the development-time curv ...200616670882
[in situ resonance raman spectra of organic pigments in freshwater cultured pearls].in situ resonance raman spectra of chinese freshwater pearls were collected and analysed systematically with three different excitation wavelengths at lambda = 514, 633 and 785 nm. at the same time, the raman spectra of eggshells of pomacea canaliculata were also collected under the same experimental conditions in order to compare with pearls' raman spectra. the conclusions were as follows: (1) frequency dispersions of raman spectra of organic pigments were obviously observed in raman spectra of ...200616827349
conchological variation in pomacea canaliculata and other south american ampullariidae (caenogastropoda, architaenioglossa). 200616972558
an integrative view to structure, function, ontogeny and phylogenetical significance of the male genital system in pomacea canaliculata (caenogastropoda, ampullariidae). 200616972560
biochemical composition, tissue origin and functional properties of egg perivitellins from pomacea canaliculata. 200616972561
facultative and obligate symbiotic associations of pomacea canaliculata (caenogastropoda, ampullariidae). 200616972562
imposex in the golden apple snail pomacea canaliculata in taiwan.the golden apple snail pomacea canaliculata (lamarck, 1822) was introduced into taiwan intentionally in the early 1980s and has become a recurring pest that seriously threatens aquatic crops. in this study, a field description of imposex with a developed penis sheath and penis in female golden apple snails from crop/domestic wastewater drainage sites and a six-order river is presented for the first time. based on the five field collections and the aquarium group, the vas deferens sequence (vds) ...200617023028
nuclear sex-determining genes cause large sex-ratio variation in the apple snail pomacea canaliculata.evolutionary maintenance of genetic sex-ratio variation is enigmatic since genes for biased sex ratios are disadvantageous in finite populations (the "verner effect"). however, such variation could be maintained if a small number of nuclear sex-determining genes were responsible, although this has not been fully demonstrated experimentally. brood sex ratios of the freshwater snail pomacea canaliculata are highly variable among parents, but population sex ratios are near unity. in this study, the ...200717057241
[studies on the growth-development and infectivity of angiostrongylus cantonensis in dormant pomacea canaliculata].to study the impact of dormancy of pomacea canaliculata on the growth-development and infectivity of angiostrongylus cantonensis.200617094596
[experimental study on compatibility of three species of freshwater snails with angiostrongylus cantonensis].to compare the compatibility of three species of freshwater snails, pomacea canaliculata, cipangopaludina chinensis, bellamy a aeruginosa, with angiostrongylus cantonensis.200617094598
astaxanthin binding and structural stability of the apple snail carotenoprotein ovorubin.ovorubin (or) is the major perivitellin of the eggs of pomacea canaliculata. the astaxanthin (asx) binding and structural stability of or were investigated by fluorescence spectroscopy and circular dichroism (cd). the apo-or (without astaxanthin) shows a single, high affinity binding site for asx (k(d)=0.5 microm). the quenching of tryptophan fluorescence by asx indicates that about 22% are near the carotenoid-binding site in a non-polar environment, as indicated by tryptophan resonance energy t ...200717324373
[development of pcr assay for detection of angiostrongylus cantonensis in pomacea canaliculata].to establish a pcr assay for detecting the third-stage larvae of angiostrongylus cantonensis in pomacea canaliculata.200617361815
variation in worm assemblages associated with pomacea canaliculata (caenogastropoda, ampullariidae) in sites near the río de la plata estuary, argentina.pomacea canaliculata is a common gastropod in freshwater habitats from central and northern argentina, extending northwards into the amazon basin. several platyhelminthes have been reported associated to p. canaliculata, sharing an intimate relationship with this gastropod host. the objectives of this study were to describe the symbiotic species assemblages associated to p. canaliculata in the study area, and to disclose differences among them. samples were taken in three typical small streams a ...200617375466
freshwater mollusks at designated areas in eleven provinces of thailand according to the water resource development projects.the study was conducted at 75 collecting loci in 15 districts of 11 provinces in thailand during 1999-2004. a total of 12,079 live mollusks were collected, 11,874 were snails and 205 were clams. the snails were comprised of 39 species and classified into 9 families: ampullariidae, bithyniidae, buccinidae, potamiopsidae, stenothyridae, thiaridae, viviparidae, planorbidae and lymnaeidae. the clams were comprised of 14 species classified into 2 families: amblemidae and corbiculidae. fifteen species ...200717539279
the identity, distribution, and impacts of non-native apple snails in the continental united states.since the mid 1990s populations of non-native apple snails (ampullariidae) have been discovered with increasing frequency in the continental united states. given the dramatic effects that introduced apple snails have had on both natural habitats and agricultural areas in southeast asia, their introduction to the mainland u.s. is cause for concern. we combine phylogenetic analyses of mtdna sequences with examination of introduced populations and museum collections to clarify the identities, intro ...200717594487
[a comparative study of three methods in detecting angiostrongylus cantonensis larvae in lung tissue of pomacea canaliculata].to compare the efficiency of three methods, lung-microscopy, tissue homogenate and enzyme digestion in the detection of angiostrongylus cantonensis larvae from the lungs of snails.200717639702
cloning, genomic organization and expression of two glycosyl hydrolase family 10 (ghf10) genes from golden apple snail (pomacea canaliculata).two cellulase cdnas (ghf10-pc1 and ghf10-pc3) belonging to glycoside hydrolase family 10 (ghf10) were successfully isolated and characterized from stomach tissue of golden apple snail (pomacea canaliculata), a kind of herbivorous mollusca. sequencing analysis revealed full-length cdnas of 1300 and 1277 bp in length, respectively. the open reading frame (orf) of cellulase cdna was 1188 and 1191 bp, encoding 395 and 396 amino acid, respectively. sequence alignment revealed that ghf10-pc1 and ghf10 ...200817852330
influence of copper on the feeding rate, growth and reproduction of the golden apple snail, pomacea canaliculata lamarck.the influence of copper on feeding rate, growth, and reproduction of pomacea canaliculata lamarck was evaluated. ten days of exposure to copper of relatively high concentration (67.5 microg/l) reduced the snails' feeding rate and retarded their growth. exposure to 20 microg/l after 36 days increased feeding rate to 28%. after 20 days of exposure at 30 microg/l, snail's growth was significant but thereafter declined. growth of all snails including control was negligible by day 50 when snails were ...200717999015
angiostrongylus cantonensis: experimental study on the susceptibility of apple snails, pomacea canaliculata compared to pila polita.six groups (15 snails/group) of pomacea canaliculata and pila polita were infected orally with 0 (control), 200, 400, 800, 1600 and 3200 first-stage angiostrongylus cantonensis larvae (l1). the respective mean+/-sd third stage larvae (l3) worm recovery 1-month post-infection (p.i.) for p. canaliculata was 0, 1.4+/-5.42 (0.7%), 0.13+/-0.35 (0.03%), 0.07+/-0.26 (0.009%), 0.07+/-0.26 (0.004%), 0, and for p. polita 0, 64.33+/-21.38 (32.25%), 115.36+/-36.82 (28.93%), 265.33+/-90.01 (33.27%), 471.33+/ ...200818154954
changes in chemical components in the freshwater apple snail, pomacea canaliculata (gastropoda: ampullariidae), in relation to the development of its cold hardiness.the apple snail, pomacea canaliculata, is an invasive freshwater snail. it increases its cold hardiness before winter. however, the physiological mechanism of cold hardiness in molluscs is poorly understood, especially in freshwater molluscs. in this study, we examined the changes in low molecular weight compounds, glycogen and lipids, in the body of p. canaliculata in association with the development of cold hardiness. when snails without cold hardiness were experimentally cold-acclimated, the ...200818190902
isolation and characterization of a novel perivitellin from the eggs of pomacea scalaris (mollusca, ampullariidae).perivitellins are important components of the perivitelline fluid (pvf) that surrounds gastropod embryos. the glyco-lipo-carotenoprotein ovorubin (or) from eggs of the snail pomacea canaliculata has been the most studied to date. here we report the characterization of scalarin (sc), a glyco-lipo-carotenoprotein from the pvf of p. scalaris. sc was purified by ultracentrifugation and exclusion chromatography. it is the major egg protein, representing 64% of the total soluble protein. the particle ...200818213678
radular ultrastructure of south american ampullariidae (gastropoda: prosobranchia).the radula of five species of south american ampullariidae was analysed by scanning electron microscope (sem) with the purpose of enlarging new studies on the systematic of this family. the studied species were pomacea canaliculata (lamarck, 1822), pomacea scalaris (d'orbigny, 1835), pomella (p.) megastoma (gray, 1847), asolene (a.) platae (maton, 1809) and felipponea neritiniformis (dall, 1919). the central tooth shows different attributes which provide the means for generic determination; the ...200718278326
fatal bacteremia due to immotile vibrio cholerae serogroup o21 in vientiane, laos - a case report.human infections with non-o1, non-o139 v. cholerae have been described from laos. elsewhere, non cholera-toxin producing, non-o1, non-o139 v. cholerae have been described from blood cultures and ascitic fluid, although they are exceedingly rare isolates.200818439249
metal contamination in the sediment, pondweed, and snails of a stream receiving effluent from a lead/zinc mine in southern china.this study assessed the potential of potamogeton crispus and pomacea canaliculata as biomonitors of sedimentary metal contamination. the results indicate p. crispus possesses several attributes of a biomonitor and its tissue concentrations of cd, pb and zn may reflect the levels of sedimentary contamination by these metals. although p. canaliculata can accumulate metals to high levels and serve as an indicator of metal contamination, its tissue metal concentrations did not correlate with those o ...200818480956
backbone cyclised peptides from plants show molluscicidal activity against the rice pest pomacea canaliculata (golden apple snail).golden apple snails ( pomacea canaliculata) are serious pests of rice in south east asia. cyclotides are backbone cyclized peptides produced by plants from rubiaceae and violaceae. in this study, we investigated the molluscicidal activity of cyclotides against golden apple snails. crude cyclotide extracts from both oldenlandia affinis and viola odorata plants showed molluscicidal activity comparable to the synthetic molluscicide metaldehyde. individual cyclotides from each extract demonstrated a ...200818557620
first egg protein with a neurotoxic effect on mice.while many invertebrates sequester toxic compounds to endow eggs with chemical defences, here we show, for the first time to our knowledge, the identification of a neurotoxin of proteinaceous nature localized inside an egg. egg extracts from the freshwater apple snail pomacea canaliculata displayed a neurotoxic effect in mice upon intraperitoneal injection (i.p.) (ld50, 96h 2.3mg/kg). egg protein and total lipids were analysed separately and the only fraction displaying a highly toxic effect (ld ...200818640143
global shape and ph stability of ovorubin, an oligomeric protein from the eggs of pomacea canaliculata.ovorubin, a 300-kda thermostable oligomer, is the major egg protein from the perivitellin fluid that surrounds the embryos of the apple snail pomacea canaliculata. it plays essential roles in embryo development, including transport and protection of carotenoids, protease inhibition, photoprotection, storage, and nourishment. here, we report ovorubin dimensions and global shape, and test the role of electrostatic interactions in conformational stability by analyzing the effects of ph, using small ...200818673387
[epidemiological analysis on 141 cases of angiostrongyliasis cantonensis in beijing].to provide scientific basis for angiostrongyliasis cantonensis control and prevention in beijing.200818785473
[epidemiological investigation of angiostrongylus cantonensis in jiangmen of guangdong province].to make an epidemiological survey on angiostrongylus cantonensis in jiangmen city of guangdong province.200819157302
[morphology of iii stage larvae of angiostrongylus cantonensis in pomacea canaliculata].to observe the morphologic characteristics of iii stage larvae of angiostrongylus cantonensis from pomacea canaliculata.200819160967
angiostrongylus cantonensis: morphological and behavioral investigation within the freshwater snail pomacea infection with angiostrongylus cantonensis, the main causative agent for human eosinophilic encephalitis, can be acquired through the consumption of the freshwater snail pomacea canaliculata. this snail also provides a suitable model to study the developmental morphology and behavior of a. cantonensis larvae, facilitated by the snail's distinct lung structure. we used microanatomy for studying the natural appearance and behavior of a. cantonensis larvae while developing within p. canaliculata ...200919172296
invasive snails and an emerging infectious disease: results from the first national survey on angiostrongylus cantonensis in china.eosinophilic meningitis (angiostrongyliasis) caused by angiostrongylus cantonensis is emerging in mainland china. however, the distribution of a. cantonensis and its intermediate host snails, and the role of two invasive snail species in the emergence of angiostrongyliasis, are not well understood.200919190771
distribution of pila polita in a southern province of thailand.this field study investigated the distribution of the freshwater snails of the family ampullariidae in 18 provinces of thailand. the introduced pomacea canaliculata has wide distribution in the studied provinces. pila angelica, p. ampullacea and p. pesmei still have limited distribution. pila polita, which is absent in the south, now can be found in phangnga, a southern province of thailand. moreover, p. polita is currently used as a traditional medicine of the local people for the treatment of ...200319230583
pila ampullacea and pomacea canaliculata, as new paratenic hosts of gnathostoma spinigerum.aquatic snails, pila ampullacea and pomacea canaliculata were experimentally found to be suitable paratenic hosts for advanced third-stage larvae (l3) of the nematode gnathostoma spinigerum, the causative parasite of gnathostomiasis in humans. g. spinigerum (l3) were found to be encapsulated in the tissue of the snail's foot and its internal organs. the infection, intensity and survival of third-stage larvae of g. spinigerum in both species of aquatic snails are described. this is the first evid ...200919323008
quantitative thin layer chromatographic analysis of the saponins in tea seed meal.the defatted seed meal of camellia oleifera abel., which contains saponins, is used extensively in aquaculture to eliminate unwanted fish and harmful insects during the cultivation of prawns, and as a molluscicide to control pomacea canaliculata lamarck. for the quality control of such tea seed meal products, a method for the determination of saponin content is required.200919333916
chance establishment for sexual, semelparous species: overcoming the allee effect.we formalize the establishment process for a sexual, semelparous organism through the use of hierarchical probability modeling from parameters of survival, probability of being female, probability of being fertilized, and expected fecundity. we show how to calculate the expected per capita growth rate and probability of extinction. an allee effect is observed if the expected population growth rate decreases as the initial population size decreases. the model can be further extended as a stochast ...200919368522
[proposed chinese name of pomacea canaliculata].the way to translate species name of pomacea canaliculata into chinese has been of confusion for a long time. we collected the relevant references on investigations of snail, species similar to pomacea canaliculata and made comparison on the characteristics of those species which serve as intermediate snail host of angiostrongylus cantonensis. it is proposed the genus name of pomacea is translated as [chinese characters: see text], and species name pomacea canaliculata be [chinese characters: se ...200919459504
development beyond the gastrula stage and digestive organogenesis in the apple-snail pomacea canaliculata (architaenioglossa, ampullariidae).development of pomacea canaliculata from the gastrula stage until the first day after hatching is described. trochophore embryos are developed after gastrulation, showing the prototroch as a crown of ciliated orange-brownish cells. however, no true veliger embryos are formed, since the prototroch does not fully develop into a velum. afterward, the connection between the fore- and midgut is permeated and the midgut becomes full of the pink-reddish albumen, which is stored into a central archenter ...200919499886
[two outbreaks of eosinophilic meningitis in yunann (china) clinical, epidemiological and therapeutical issues].eosinophilic meningitis is an uncommon clinical entity, which is mostly caused by angiostrongylus cantonensis, a nematode which parasitizes rat's lungs. humans represent a dead-end in the parasite's lifecycle and become infected by ingesting snails, slugs or transport hosts. due to uncontrolled proliferation and circulation of snails and rats, human angiostrongyliasis has emerged in new foci in continental china. the treatment, which relies upon a combination of albendazole and corticosteroids, ...200919583024
physiological response to low temperature in the freshwater apple snail, pomacea canaliculata (gastropoda: ampullariidae).cold hardiness of the freshwater apple snail, pomacea canaliculata, varies seasonally. we investigated lethal factors and physiological changes arising from exposure of p. canaliculata to low temperatures. snails did not survive freezing. the supercooling point of cold-acclimated (cold tolerant) snails (-6.6+/-0.8 degrees c) did not differ significantly from that of non-acclimated ones (-7.1+/-1.5 degrees c) under laboratory conditions. furthermore, snails died even under more moderately low tem ...200919648400
human angiostrongyliasis outbreak in dali, china.several angiostrongyliasis outbreaks have been reported in recent years but the disease continues to be neglected in public health circles. we describe an outbreak in dali, southwest china in order to highlight some key problems for the control of this helminth infection.200919771154
use of a saponin based molluscicide to control pomacea canaliculata snails in southern brazil.pomacea canaliculata snails pose a severe problem to direct seeded rice cultivated in southern brazil. control of this snail is nowadays performed with toxic chemicals such as copper sulfate and fungicides such as fentin. a novel natural molluscicide based on alkali modified quinoa (chenopodium quinoa) saponins was tested under laboratory conditions. snails were collected in rice fields close to porto alegre (state of rio grande do sul) and in brusque (state of santa catarina, 400 km north of po ...200919911565
structure and stability of the neurotoxin pv2 from the eggs of the apple snail pomacea canaliculata.there is little information on the egg proteins of gastropod mollusks. here we focus on pv2, a novel neurotoxin from snail eggs, studying its size, shape, structure, and stability, using small angle x-ray scattering (saxs), absorption and fluorescence spectroscopy, circular dichroism, electron microscopy and partial proteolysis. results indicate that pv2 is a compact and well folded oligomer of 130x44 a. it is an octamer of four 98 kda heterodimers composed of 67 and 31 kda subunits. subunits ar ...201020215051
[the first national survey on natural nidi of angiostrongylus cantonensis in china].to reveal the natural distribution of angiostrongylus cantonensis in the mainland of china.200920232636
[survey on the focus of angiostrongylus cantonensis in guangdong province].to understand the distribution of angiostrongylus cantonensis foci in guangdong province for making surveillance program.201020411741
protein expression during the embryonic development of a gastropod.despite the potential use of gastropod embryos in basic and applied research, little is known about their protein expression. we examined, for the first time, changes in proteomic profile during embryonic development of pomacea canaliculata from an embryo without a shell (stage ii) to an embryo with a fully formed shell (stage iii) to understand the roles that proteins play in critical developmental events, such as the formation of shell, operculum and heart, and the differentiation of head and ...201020455212
snails and trematode infection after indian ocean tsunami in phang-nga province, southern thailand.the tsunami and non-tsunami affected areas of takua pa district, phang-nga province were investigated for fresh- and brackish-water snails that transmit human parasitic diseases during 2006 and 2007. among 46 snail species found, 17 species of 8 families were freshwater snails, 28 species of another 7 families were brackish-water snails, and 1 species was a land snail. of these species, 11 freshwater snails, 4 brackish-water snails and 1 land snail were of medical importance. the fresh-water sna ...201020578482
induced chemical defenses in a freshwater macrophyte suppress herbivore fitness and the growth of associated microbes.the freshwater macrophyte cabomba caroliniana induces a chemical defense when attacked by either the crayfish procambrus clarkii or the snail pomacea canaliculata. induction by either consumer lowers the palatability of the plant to both consumers. when offered food ad libitum, snails feeding on non-induced c. caroliniana grew 2.6-2.7 times more than those feeding on induced c. caroliniana. because snails fed less on induced plants, this could be a behavioral effect (reduced feeding), a physiolo ...201020927537
the role of the proteinase inhibitor ovorubin in apple snail eggs resembles plant embryo defense against predation.fieldwork has thoroughly established that most eggs are intensely predated. among the few exceptions are the aerial egg clutches from the aquatic snail pomacea canaliculata which have virtually no predators. its defenses are advertised by the pigmented ovorubin perivitellin providing a conspicuous reddish coloration. the nature of the defense however, was not clear, except for a screening for defenses that identified a neurotoxic perivitellin with lethal effect on rodents. ovorubin is a proteina ...201021151935
a new dimeric iridal triterpenoid from belamcanda chinensis with significant molluscicide unprecedented dimeric triterpenoid, designated dibelamcandal a, with a six-membered ring linking two iridal type triterpenoid nuclei, was isolated from the rhizome of belamcanda chinensis. its structure was determined by extensive spectroscopic measurements, including ir, esi-ms, hr-esi-ms, and 1d and 2d nmr. it demonstrated significant molluscicide activity against pomacea canaliculata.201021174449
uric acid deposits and estivation in the invasive apple-snail, pomacea canaliculata.the physiological ability to estivate is relevant for the maintenance of population size in the invasive pomacea canaliculata. however, tissue reoxygenation during arousal from estivation poses the problem of acute oxidative stress. uric acid is a potent antioxidant in several systems and it is stored in specialized tissues of p. canaliculata. changes in tissue concentration of thiobarbituric acid reactive substances (tbars), uric acid and allantoin were measured during estivation and arousal in ...201021182978
epidemiological survey of angiostrongylus cantonensis in the west-central region of guangdong province, china.the study was to understand the angiostrongylus cantonensis infectious situation of rodent definitive host, snail intermediate host, and local residents in the west-central region of guangdong province in china. the snails achatina fulica and pomacea canaliculata collected from the survey place were digested with artificial gastric juice, and the third-stage larvae of a. cantonensis in the snails were examined under microscope. the heart and lung of rats captured from the survey place were taken ...201121301874
[multiplex pcr assay for the detection of angiostrongylus cantonensis larvae in pomacea canaliculata].to establish a multiplex pcr assay for detecting angiostrongylus cantonensis larvae in pomacea canaliculata.201021351548
pomacea canaliculata (mollusca, gastropoda) in patagonia: potential role of climatic change in its dispersion and settlement.pomacea canaliculata (lamarck, 1822) (mollusca gastropoda) shows a large native distribution range in south america, reaching as far south as 37-¦ s (buenos aires, argentina). this species was deliberately introduced into southeast asia around 1980 and subsequently underwent a rapid intentional or accidental dispersal into many countries in the region. it was also introduced into north and central america and hawaii. in this contribution we record the presence of p. canaliculata in patagonia, as ...201121437394
histopathological effects of contaminated sediments on golden apple snail (pomacea canaliculata, lamarck 1822).pomacea canaliculata were exposed experimentally to contaminated sediments from a tributary of the mae klong river, thailand, for 3 months. the highest concentration of cr, zn and fe accumulated in the digestive gland while the gill exhibited the highest concentration of cu. in addition, histopathological changes (increased mucus vacuoles, loss of cilia, dilation of cells in the epithelial cells of digestive tract organs, and an increase in the number of dark granules in the digestive cells) wer ...201121516297
toxicity of botanical insecticides on golden apple snail (pomacea canaliculata).the molluscicidal activity of crude extracts from five highly potential plants, annona squamosa seed, nerium indicum leaves, stemona tuberose root, cyperus rotundus corm and derris elliptica root was assessed to pomacea canaliculata. d. elliptica root and c. rotundus corm extracts showed the highest toxicity against 3-month old snails which have lc50 as 23.68 +/- 2.96 mg/l and 133.20 +/- 7.94 mg/l, respectively. the c. rotundus corm extracts were chosen for detoxification enzyme in vivo assay wh ...201021542482
epidemic status of angiostrongylus cantonensis in hainan island, understand the current epidemiological status of angiostrongylus cantonensis in hainan island and provide the scientific evidences for prevention and control of the disease.201121771469
molluscicidal activity of cardiac glycosides from nerium indicum against pomacea canaliculata and its implications for the mechanisms of toxicity.cardiac glycosides from fresh leaves of nerium indicum were evaluated for its molluscicidal activity against pomacea canaliculata (golden apple snail: gas) under laboratory conditions. the results showed that lc(50) value of cardiac glycosides against gas was time dependent and the lc(50) value at 96h was as low as 3.71mg/l, which was comparable with that of metaldehyde at 72h (3.88mg/l). these results indicate that cardiac glycosides could be an effective molluscicide against gas. the toxicolog ...201121843803
First provincial survey of Angiostrongylus cantonensis in Guangdong Province, China.The rat lungworm Angiostrongylus cantonensis is a zoonotic nematode with a wide distribution. We report the first provincial survey of the prevalence of A. cantonensis infection among wild rodents and snails in Guangdong Province, China. A total of 2929 Pomacea canaliculata and 1354 Achatina fulica were collected from fields in 22 survey sites with a larval infection rates ranging from 0-26.6% to 0-45.4%. In addition, 114 Cipangopaludina sp and 252 Bellamya sp were bought from markets; larvae we ...201121906215
two new triterpenoids from the bark of eucalyptus exserta and their molluscicidal and cytotoxic activities.two new triterpenoids, 2α-hydroxyurs-12-en-28-oic acid-3β-trans- isoferulate (1) and 2α, 3α, 24-trihydroxyolean-18-en-28-oic acid (2), together with three known triterpenoids (3-5) were isolated from the methanol extract of the bark of eucalyptus exserta. their structures were determined by spectroscopic means. compounds 1, 2, and 5 exhibited molluscicidal activity against pomacea canaliculata. all isolated compounds showed significant cytotoxic activity against spodoptera litura (sl) cells.201122178417
Loop-mediated isothermal amplification: rapid detection of Angiostrongylus cantonensis infection in Pomacea canaliculata.Angiostrongylus cantonensis is a zoonotic parasite that causes eosinophilic meningitis in humans. The most common source of infection with A. cantonensis is the consumption of raw or undercooked mollusks (e.g., snails and slugs) harbouring infectious third-stage larvae (L3). However, the parasite is difficult to identify in snails. The purpose of this study was to develop a quick, simple molecular method to survey for A. cantonensis in intermediate host snails.201122023992
isolation and characterization of sixteen polymorphic microsatellite loci in the golden apple snail pomacea canaliculata.we report the characterization of 16 polymorphic microsatellite markers in the golden apple snail, pomacea canaliculata, a pest registered in the list of "100 of the world's worst invasive alien species". the fast isolation by aflp (amplified fragment length polymorphism) of sequences containing repeats (fiasco) method was used to isolate microsatellite loci, and polymorphism was explored with 29 individuals collected in an invasive region from china. these primers showed a number of alleles per ...201122016640
an outbreak of angiostrongyliasis in guanging, people's republic of china: migrants vulnerable to an emerging disease.angiostrongyliasis has been frequently reported from the people's republic of china during the last decade. an outbreak of angiostrongyliasis among migrant laborers in guangning, guangdong province is described here. a questionnaire was developed to collect epidemiological and clinical information about 17 migrant laborers from the bai ethnic group in dali, yunnan province. serum samples were collected and tested by enzyme-linked immunosorbent assay. rats and mollusks from the same area where pa ...201122299428
molecular characteristics of the hsp70 gene and its differential expression in female and male golden apple snails (pomacea canaliculata) under temperature stimulation.heat-shock protein 70 (hsp70) is one of the most important heat-shock proteins that helps organisms to modulate stress response via over-expression. the hsp70 gene from pomacea canaliculata was cloned using the race approach; the gene is 2,767 bp in length and contains an open reading frame of 1,932 bp, which is encoded by a polypeptide of 643 amino acids. blast analysis showed that the predicted amino acid sequence of the p. canaliculata hsp70 gene shared a relatively high similarity with that ...201324368711
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