design and application of two novel degenerate primer pairs for the detection and complete genomic characterization of potyviruses. | two pairs of degenerate primers were designed from sequences within the potyviral ci (cifor/cirev) and hc-pro-coding regions (hpfo/hprev), and these were shown to be highly specific to members of the genus potyvirus. using the cifor and cirev primers, three novel potyviruses infecting crop and weed species from vietnam were detected, namely telosma mosaic virus (telmv) infecting telosma (telosma cordata, asclepiadaceae), peace lily mosaic virus (pelmv) infecting peace lily (spathiphyllum patinii ... | 2008 | 17906831 |
complete genome sequence of a tobacco isolate of the tobacco vein banding mosaic virus strain prevailing in china. | tobacco vein banding mosaic virus (tvbmv) is a species of the largest plant virus genus potyvirus. its incidence has been increasing in chinese tobacco-growing area. tvbmv isolates can be clustered into three genetic groups that are corresponding with their geographical origin. we have reported the complete genomic sequence of tvbmv isolate ynd with unique nib/cp cleavage site. here, we determined and analyzed the complete genomic sequence of isolate hn39, which was collected from tobacco in hen ... | 2010 | 20012876 |
the complete genome sequence of a brazilian isolate of yam mild mosaic virus. | in this study, the complete genome of an isolate of yam mild mosaic virus (ymmv) from brazil was sequenced, and the predicted amino acid sequence was analyzed. the ymmv rna genome consists of 9538 nt without the poly(a) tail, encoding a putative typical potyvirus polyprotein of 3084 amino acids. furthermore, the small overlapping orf (pipo) in the p3 gene was also deduced, and the cleavage sites of the polyprotein were predicted. multiple alignment with other potyviruses showed a maximum nucleot ... | 2013 | 23081678 |