| pseudomonas reinekei sp. nov., pseudomonas moorei sp. nov. and pseudomonas mohnii sp. nov., novel species capable of degrading chlorosalicylates or isopimaric acid. | three bacterial strains, designated mt1(t), rw10(t) and ipa-2(t), had been isolated previously for their ability to degrade chlorosalicylates or isopimaric acid. 16s rrna gene sequence analysis demonstrated that these bacteria are related to species of the genus pseudomonas. analysis of the results of dna-dna hybridization with several close phylogenetic neighbours revealed a low level of hybridization (less than 57 %). on the basis of phenotypic characteristics, phylogenetic analysis, dna-dna r ... | 2007 | 17473234 |
| community-based degradation of 4-chorosalicylate tracked on the single cell level. | 4-chlorosalicylate (4-cs) can be degraded completely by a bacterial consortium consisting of pseudomonas reinekei (mt1), achromobacter spanius (mt3) and pseudomonas veronii (mt4). the fourth species wautersiella falsenii (mt2) is thought to act as a 'necrotizer' of the community. single cell approaches were used to follow every species' degradation activity within the community by assuming that growth and proliferation are activity markers for the utilization of 4-cs and its degradation pathway ... | 2008 | 18593643 |
| trans-dienelactone hydrolase from pseudomonas reinekei mt1, a novel zinc-dependent hydrolase. | pseudomonas reinekei mt1 is capable of growing on 4- and 5-chlorosalicylate, involving a pathway with trans-dienelactone hydrolase (trans-dlh) as a key enzyme. it acts on 4-chloromuconolactone formed during cycloisomerization of 3-chloromuconate by hydrolyzing it to maleylacetate. the gene encoding this activity was localized, sequenced and expressed in escherichia coli. inductively coupled plasma mass spectrometry showed that both the wild-type as well as recombinant enzymes contained 2 moles o ... | 2008 | 18789896 |
| bacterial consortium proteomics under 4-chlorosalicylate carbon-limiting conditions. | in this study, the stable consortium composed by pseudomonas reinekei strain mt1 and achromobacter xylosoxidans strain mt3 (cell numbers in proportion 9:1) was under investigation to reveal bacterial interactions that take place under severe nutrient-limiting conditions. the analysis of steady states in continuous cultures was carried out at the proteome, metabolic profile, and population dynamic levels. carbon-limiting studies showed a higher metabolic versatility in the community through upreg ... | 2009 | 19382143 |
| characterization of a gene cluster involved in 4-chlorocatechol degradation by pseudomonas reinekei mt1. | pseudomonas reinekei mt1 has previously been reported to degrade 4- and 5-chlorosalicylate by a pathway with 4-chlorocatechol, 3-chloromuconate, 4-chloromuconolactone, and maleylacetate as intermediates, and a gene cluster channeling various salicylates into an intradiol cleavage route has been reported. we now report that during growth on 5-chlorosalicylate, besides a novel (chloro)catechol 1,2-dioxygenase, c12o(ccaa), a novel (chloro)muconate cycloisomerase, mci(ccab), which showed features no ... | 2009 | 19465655 |
| crystal structure and catalytic mechanism of 4-methylmuconolactone methylisomerase. | when methyl-substituted aromatic compounds are degraded via ortho (intradiol)-cleavage of 4-methylcatechol, the dead-end metabolite 4-methylmuconolactone (4-ml) is formed. degradation of 4-ml has only been described in few bacterial species, including pseudomonas reinekei mt1. the isomerization of 4-ml to 3-methylmuconolactone (3-ml) is the first step required for the mineralization of 4-ml and is catalyzed by an enzyme termed 4-methylmuconolactone methylisomerase (mlmi). we identified the gene ... | 2009 | 19801657 |
| novel metal-binding site of pseudomonas reinekei mt1 trans-dienelactone hydrolase. | pseudomonasreinekei mt1 is capable of growing on 4- and 5-chlorosalicylate as the sole carbon source involving a pathway with trans-dienelactone hydrolase as the key enzyme. this enzyme transforms 4-chloromuconolactone to maleylacetate and thereby avoids the spontaneous formation of toxic protoanemonin. trans-dienelactone hydrolase is a zn(2+)-dependent hydrolase where activity can be modulated by the exchange of zn(2+) by mn(2+) in at least two of the three metal-binding sites. site directed va ... | 2009 | 19895788 |
| modified 3-oxoadipate pathway for the biodegradation of methylaromatics in pseudomonas reinekei mt1. | catechols are central intermediates in the metabolism of aromatic compounds. degradation of 4-methylcatechol via intradiol cleavage usually leads to the formation of 4-methylmuconolactone (4-ml) as a dead-end metabolite. only a few microorganisms are known to mineralize 4-ml. the mml gene cluster of pseudomonas reinekei mt1, which encodes enzymes involved in the metabolism of 4-ml, is shown here to encode 10 genes found in a 9.4-kb chromosomal region. reverse transcription assays revealed that t ... | 2010 | 20061479 |
| consortia modulation of the stress response: proteomic analysis of single strain versus mixed culture. | the high complexity of naturally occurring microbial communities is the major drawback limiting the study of these important biological systems. in this study, a comparison between pure cultures of pseudomonas reinekei sp. strain mt1 and stable community cultures composed of mt1 plus the addition of achromobacter xylosoxidans strain mt3 (in a steady-state proportion 9:1) was used as a model system to study bacterial interactions that take place under simultaneous chemical and oxidative stress. b ... | 2010 | 20406297 |
| kinetics of carbon sharing in a bacterial consortium revealed by combining stable isotope probing with fluorescence-activated cell sorting. | aims: to determine the kinetics of substrate fluxes in a microbial community in order to elucidate the roles of the community members. methods and results: the kinetics of substrate sharing in a bacterial consortium were measured by a new analytical approach combining immunostaining, stable isotope probing and fluorescence-activated cell sorting (facs). the bacterial consortium, consisting of four strains and growing on 4-chlorosalicylate (4-cs), was pulse-dosed with the degradation intermedia ... | 2011 | 21299770 |
| pseudomonas baetica sp. nov., a novel fish pathogen isolated from wedge sole, dicologoglossa cuneata (moreau). | five gram negative bacterial isolates recovered from an outbreak occurred in march 2006 in huelva, spain, affecting adult diseased cultured wedge sole (dicologoglossa cuneata), were phenotypically and genotypically characterized in order to clarify its taxonomic position. on the basis of 16s rrna gene sequence analysis isolates were included in the genus pseudomonas, within the p. fluorescens-related species group, being their closest relatives the p. jessenii and p. koreensis subgroups. the hig ... | 2011 | 21642488 |
| degradation of 2,3-dihydroxybenzoate by a novel meta-cleavage pathway. | 2,3-dihydroxybenzoate is the precursor in the biosynthesis of several siderophores and an important plant secondary metabolite that, in bacteria, can be degraded via meta-cleavage of the aromatic ring. the dhb cluster of pseudomonas reinekei mt1 encodes a chimeric meta-cleavage pathway involved in the catabolism of 2,3-dihydroxybenzoate. while the first two enzymes, dhba and dhbb, are phylogenetically related to those involved in 2,3-dihydroxy-p-cumate degradation, the subsequent steps are catal ... | 2012 | 22609919 |
| isolation of a thioesterase gene from the metagenome of a mountain peak, apharwat, in the northwestern himalayas. | the soil metagenome of apharwat (latitude 34.209° and longitude 74.368°) was explored for the presence of esterase encoding genes using a cultivation-independent approach, metagenomics. among the various protocols tested, the method developed by wechter was found to be the best for metagenome isolation from the soil under investigation. the purity of the isolated metagenomic dna was not suitable for gene cloning. to improve the yield and purity of isolated metagenomic dna, isothermal amplificati ... | 2013 | 28324349 |
| management of meloidogyne incognita on tomato with endophytic bacteria and fresh residue of wasabia japonica. | to characterize the nematicidal endophytic bacteria (neb) of wasabia japonica (wasabi) and evaluated the control efficacies of promising neb as well as fresh wasabi residue (fwr) against meloidogyne incognita on tomato. | 2014 | 24965702 |
| protoanemonin: a natural quorum sensing inhibitor that selectively activates iron starvation response. | many gram-negative bacteria employ cell-to-cell communication mediated by n-acyl homoserine lactones (quorum sensing) to control expression of a wide range of genes including, but not limited to, genes encoding virulence factors. outside the laboratory, the bacteria live in complex communities where signals may be perceived across species. we here present a newly found natural quorum sensing inhibitor, produced by the pseudomonads pseudomonas sp. b13 and pseudomonas reinekei mt1 as a blind end i ... | 2013 | 22672701 |