| specific detection and quantification of the phytopathogenic agent 'candidatus phytoplasma prunorum'. | 'candidatus phytoplasma prunorum' is a wall-less bacterium associated with european stone fruit yellows (esfy), a severe disease of prunus spp. (mainly apricot and japanese plum trees). it can be spread by one insect vector, cacopsylla pruni, and by the trade of infected material. the availability of pcr-based methods allowing a sensitive and specific detection of 'ca. p. prunorum' is crucial for this phytoplasma because, at present, it is uncultured and cannot be detected serologically. we deve ... | 2009 | 19401232 |
| nine polymorphic microsatellite loci from the psyllid cacopsylla pruni (scopoli), the vector of european stone fruit yellows. | cacopsylla pruni is the vector of european stone fruit yellows, a quarantine disease of prunus trees. nine polymorphic microsatellite markers were developed from enriched dna libraries. allelic variability was assessed in a collection of 149 females obtained from five localities covering a large geographical area in france. the number of detected alleles ranged from 8 to 37. within the localities, observed and expected heterozygosities averaged across loci ranged from 0.39 to 0.55, and from 0.68 ... | 2009 | 21564874 |
| efficient transmission of 'candidatus phytoplasma prunorum' is delayed by eight months due to a long latency in its host-alternating vector. | understanding at which spatiotemporal scale a disease causes significant secondary spread has both theoretical and practical implications. we investigated this issue in the case of european stone fruit yellows (esfy), a quarantine vector-borne phytoplasma disease of prunus trees. our work was focused on the processes underlying disease spread: the interplay between the life cycles of the pathogen ('candidatus phytoplasma prunorum') and of the vector (cacopsylla pruni). we demonstrated experiment ... | 2009 | 19203279 |
| identifying risk factors for european stone fruit yellows from a survey. | abstract european stone fruit yellows (esfy) is becoming a major economic problem for prunus growers in europe. the causal agent ("candidatus phytoplasma prunorum") and its vector (cacopsylla pruni) have been identified, but the present knowledge of the risk factors for this disease relies, at best, on specific experiments. to assess the relative significance of several factors correlated with esfy incidence in the field, an exhaustive survey was performed on apricot and japanese plum orchards i ... | 2006 | 18943755 |
| molecular test to assign individuals within the cacopsylla pruni complex. | crop protection requires the accurate identification of disease vectors, a task that can be made difficult when these vectors encompass cryptic species. here we developed a rapid molecular diagnostic test to identify individuals of cacopsyllapruni (scopoli, 1763) (hemiptera: psyllidae), the main vector of the european stone fruit yellows phytoplasma. this psyllid encompasses two highly divergent genetic groups that are morphologically similar and that are characterized by genotyping several micr ... | 2013 | 23977301 |