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fascioloides magna: development in selected nonruminant mammalian hosts. 1979108129
the penetration of fascioloides magna miracidia into the snail host fossaria bulimoides. a scanning electron microscope study.snails of the species fossaria bulimoides were exposed to miracidia of fascioloides magna for given periods of time. the course of penetration was followed by means of scanning electron microscopy. topographical features of the miracidial morphology were studied; the apical organ, epidermal plates and their shedding, and body shapes during penetration. preliminary data were obtained concerning the nature of the lesions formed by the miracidia. inferences were drawn concerning the mode and durati ...1977888510
fascioloides magna (bassi, 1875) in feral swine from southern texas.between 1971 and 1975, fascioloides magna was found in 46 of 67 (69%) feral swine (sus scrofa) in southern texas. flukes were recovered from swine in areas where f. magna commonly has been recovered from white-tailed deer and cattle. one to 12 flukes were recovered from each infected animal. their presence was indicated by black hematin pigment on the liver and various other internal organs. eggs were not detected in the gallbladder or feces of infected animals although mature flukes and eggs we ...19751195502
efficacy of triclabendazole against natural infections of fascioloides magna in wapiti.fourteen free-ranging adult wapiti (cervus elaphus nelsoni) were captured in banff national park, alberta (canada) and held in captivity near edmonton. a 24% suspension of triclabendazole at doses of 30 to 100 mg/kg body weight was drenched into the rumen of eight females and four males. two male wapiti were used as untreated controls. animals were killed and examined at 4 (n = 3), 6 (n = 4), or 8 (n = 4) wk after treatment. efficacy was 90% against immature fascioloides magna collected 4 wk aft ...19911758025
domestic sheep as a rare definitive host of the large american liver fluke fascioloides magna.two domestic sheep were inoculated orally with 250 metacercariae of fascioloides magna. one sheep died 4 mo after inoculation and 22 immature f. magna were recovered. the second sheep remained healthy and was killed 8 mo after inoculation. ten mature f. magna were recovered from hepatic parenchyma, and eggs of f. magna were observed in areas of the liver where flukes resided and from feces. although f. magna is almost always lethal to sheep, this sheep was a rare definitive host.19902213420
use of albendazole in feed to control fascioloides magna infections in captive white-tailed deer (odocoileus virginianus).thirty-six adult white-tailed deer (odocoileus virginianus) naturally infected with fascioloides magna were captured and randomly assigned to four groups. each group was fed pelleted feed coated with albendazole for each of seven consecutive days to deliver the drug at a dose rate of approximately 0.0, 5.0, 8.5, or 16.5 mg/kg bodyweight/day. at 7 wk posttreatment, each animal was euthanized and necropsied. effects of albendazole treatment included significant reduction (p less than 0.05) in para ...19902338728
survey of hepatic and pulmonary helminths of wild cervids in alberta, canada.during the 1988 hunting season, livers and lungs from 263 mule deer (odocoileus hemionus hemionus), 198 moose (alces alces), 147 white-tailed deer (odocoileus virginianus), and 94 wapiti (cervus elaphus nelsoni) from alberta (canada) were collected for parasitological examination. most of the samples (89%) were submitted by big game hunters throughout the province. giant liver fluke (fascioloides magna) was found in 9% of 22 yearling and 29% of 65 adult wapiti; 4% of 161 adult moose; and 2% of 9 ...19902250321
hematology and clinical pathology of experimental fascioloides magna infection in cattle and guinea pigs.the hematologic and clinico-pathologic response to fascioloides magna infection in cattle and guinea pigs was investigated. twelve calves (six infected and six controls) were monitored for 26 weeks after inoculation with 1000 metacercariae. all calves remained healthy and there were no significant differences in weight gains between infected and control groups. flukes (mean = 9.2, range 1-32) were recovered from the liver and abdominal cavity of all infected calves. the only significant response ...19911788931
production and characterization of monoclonal antibodies against excretory-secretory products of fasciola hepatica.four monoclonal antibodies (mabs) (9.49, 24.27, 46.71 and 179.57) were produced against fasciola hepatica excretory-secretory products. isotype analysis revealed the antibodies to be igm, igg3, igg1, and igm. in immunoblot assays, the mabs recognized different antigenic polypeptides migrating between 29 and 180 kda. specificity of the mabs was evaluated by elisa against antigens of fascioloides magna, anoplocephala magna, stichorchis subtriquetrus, haemonchus contortus, sheep liver extract (sle) ...19911788930
hepatic and pulmonary pathology of experimental fascioloides magna infection in guinea pigs.the guinea pig was used to study the pathology of fascioloides magna, an important pathogen for sheep. although flukes migrated freely through various tissues in infected guinea pigs, the most serious lesions occurred in the liver and lungs. the sequential development of lesions indicated that flukes first invaded the quadrate lobe of the liver and subsequently migrated to other liver lobes and tissues. six weeks post-infection, there was a marked drop in the recovery of flukes from the liver al ...19911779042
experimental fascioloides magna infections of mule deer (odocoileus hemionus hemionus).six mule deer (odocoileus hemionus hemionus) and one white-tailed deer (odocoileus virginianus), approximately 5-mo-old, each were inoculated orally with 500 metacercariae of fascioloides magna. all mule deer died from liver fluke infection between 69 and 134 days (mean = 114, se = 9.9) after inoculation. between 38 and 326 immature f. magna (mean = 102, se = 45.5) were recovered from each deer at necropsy. flukes were present in livers, lungs, and free in pleural and peritoneal spaces. infectio ...19921602568
isoelectric focusing of soluble proteins from fasciola hepatica l, 1758 and fascioloides magna b, 1875.isoelectric focusing was performed on the soluble proteins of whole-body and excretory-secretory products (esp) of fasciola hepatica and fascioloides magna. adult f hepatica flukes were recovered from experimentally infected sheep and esp obtained from the flukes; portions of liver were cut and frozen at -70 c. fascioloides magna adults were collected from naturally infected white-tailed deer and esp obtained; portions of liver were collected from noninfected white-tailed deer. adult flukes and ...19921575393
efficacy of two formulations of albendazole against liver flukes in cattle.albendazole (10 mg/kg of body weight) was administered as a drench suspension or as a feed additive to 24 cattle with naturally acquired infections of fasciola hepatica and fascioloides magna. cattle were euthanatized 16 to 30 days after treatment, and the number of viable flukes was counted. viable f hepatica and f magna were decreased by 91.4% and 70.6% for drench administration and by 82.9% and 71.9% for the feed additive treatment, respectively. there was no significant difference between th ...19921497187
comparative efficacy of clorsulon and albendazole against fasciola hepatica in cattle.in a dosage-confirmation trial, anthelmintic activities of clorsulon and albendazole against fasciola hepatica were evaluated and compared. twenty-eight cattle (8 to 12 months old) with natural f hepatica infections were randomly allotted to 4 groups of 7 cattle each: group 1, no treatment (controls); group 2, clorsulon suspension given orally at 3.5 mg/kg of body weight; group 3, clorsulon suspension given orally at 7 mg/kg; and group 4, albendazole paste given orally at 10 mg/kg. at necropsies ...19854026039
pathophysiologic effects of experimentally induced fascioloides magna infection in sheep.the pathophysiologic responses of 13 sheep inoculated orally with 100 metacercariae of fascioloides magna were monitored for 4 months after inoculation. there were no differences in weight gains between these and a number of noninoculated control sheep throughout the experiment. complete blood cell counts showed an increase only in the absolute number of eosinophils. serum preparations (2 times a week) from 7 inoculated and 7 noninoculated sheep did not identify any significant changes in alkali ...19854037489
efficacy of clorsulon and albendazole against fascioloides magna in naturally infected white-tailed deer.the efficacy of clorsulon and of albendazole against fascioloides magna were evaluated in 36 naturally infected white-tailed deer (odocoileus virginianus) in southern texas. a single oral dose of clorsulon suspension (12 to 30 mg/kg of body weight; mean = 24 mg/kg) was given to each deer and killed 153 (92%) of 167 mature flukes and 4 (80%) of 5 immature flukes recovered at necropsy. a single oral dose of albendazole paste (17 to 46 mg/kg; mean = 26 mg/kg) was given to each deer and killed 148 ( ...19854077630
epizootiology of fascioliasis in montana.during 1989-1990, the united states department of agriculture (usda) meat inspection records were used to determine the distribution and incidence of liver flukes (fasciola hepatica and fascioloides magna) in montana cattle. of the cows and bulls slaughtered in usda-inspected packing plants during a 12 month time period, 17.24% had livers that were condemned because of liver flukes. this was a 12% increase over usda liver condemnations reported for 1973. infected animals have been reported from ...19921496783
detection of stable diagnostic antigen from bile and feces of fasciola hepatica infected cattle.diagnostic antigens in bile and feces from fasciola hepatica infected cattle were detected and characterized by enzyme-linked immunotransfer blot (eitb) techniques. as sources of antigen, samples of bile, intestinal contents and feces were collected from five uninfected calves and from 10 calves with known fasciola hepatica burdens. a band detected by eitb using a densitometer in the area corresponding to 26 kda reacted with rabbit anti-fresh fluke antigen and infected cattle sera but not with f ...19921485418
development of the large american liver fluke, fascioloides magna, in white-tailed deer, cattle, and sheep.the comparative development of fascioloides magna in white-tailed deer, cattle and sheep has been studied. flukes were recovered from 72% of 32 deer administered 40 to 500 metacercariae, from 82% of 11 cattle administered 10 to 500 metacercariae, and from 53% of 15 sheep administered 8 to 200 metacercariae. the percentage recovery of the flukes administered as metacercariae was 4.1% of 6,130 in deer, 5.7% of 2,510 in cattle, and 4.7% of 1,213 in sheep. flukes were recovered only from livers of i ...19761255380
parenteral infection of white-tailed deer (odocoileus virginianus) with metacercariae of fasciola hepatica and fascioloides magna. 19761255364
fascioloides magna infection in white-tailed deer of southeastern united states.livers of 4,023 white-tailed deer (odocoileus virginianus) from 13 southeastern states were examined for flukes; 514 (12.8%) harbored fascioloides magna. other species of liver flukes were not found. forty-two nidi of infection were found, encompassing 56 counties in 9 states. as many as 125 flukes were recovered from a single deer. although f magna was widely distributed throughout southeastern united states, most enzootic areas were confined to the coastal plain physiographic province. since d ...1977924869
[clinical examination of the blood of red deer (cervus elaphus l.) naturally infested with parasites].in six animals hunted and four immobilized animals of red deer (cervus elaphus l.) of both sexes and of different age, kept at three game preserves in bohemia, the psychological values were ascertained: the number of erythrocytes and leucocytes, the hemoglobin content, the hematocrit, mcv, mch, mchc, the white blood count, the total serum protein, the fractions: albumin, alpha-, beta- and gamma-globulin, the activity of sgot, and sgpt, the alkaline phosphatase and the metabolism of calcium, phos ...1976820034
anthelmintic activity of albendazole in white-tailed deer.albendazole at dose rates of 11 to 54 mg/kg (mean of 25 +/- 11 sd) of body weight was highly effective (greater than 99%) against haemonchus contortus, ostertagia odocoilei, o mossi, trichostrongylus askivali and nematodirus odocoilei in 22 white tailed deer (odocoileus virginianus) in southern texas. it was 38% effective against mature and immature deer liver flukes (fascioloides magna). toxicoses associated with treatment were not observed. large numbers of abomasal nematodes (mean of 2,949 +/ ...1978749572
experimental infection of lymnaeid snails in wisconsin with miracidia of fascioloides magna and fasciola hepatica. 1978739308
first report of fascioloides magna (bassi, 1875) in south africa.fascioloides magna from a brahman heifer recently imported from the usa is recorded for the first time in south africa. the pathological findings are briefly described. the possibility of this parasite becoming established in this country is also discussed.1977614520
fascioloides magna in white-tailed deer (odocoileus virginianus): observations on the pairing tendency.fascioloides magna (bassi 1875) was recovered from 227 of 312 (73%) white-tailed deer (odocoileus virginianus) in southern texas between 1971 and 1975. deer from age classes more than 1 year old had infection rates of from 64 to 84%. few dead flukes were found. flukes occurred exclusively in the livers of infected animals and were paired in 256 of 301 (85%) fibrous hepatic capsules. the prevalence of immature flukes with an average of one immature per infected liver was similar in all age classe ...1977592035
fascioloides magna (bassi, 1875) ward, 1917 (trematoda) in woodland caribou, rangifer tarandus caribou (gmelin), of northeastern quebec, and its distribution in wild ungulates in canada. 19715101972
a controlled evaluation of albendazole against natural infections with fasciola hepatica and fascioloides magna in cattle.albendazole administered to cattle at doses of 45, 35, 25, and 15 mg/kg of body weight reduced the number of viable fasciola hepatica adults by 99.8%, 97.6%, 97.6%, and 95.7%, f hepatica immatures by 100%, 98.6%, 98.6% and 91.9% and fascioloides magna by 96.5%, 99.1%, 94.0%, and 94.0%, respectively. statistical evaluation by one-way analysis of variance, showed significant (p less than 0.05) differences in burdens of each fluke species between treated and untreated cattle, but differences in eff ...1979525936
histochemical localization of cholinesterase activity in fascioloides magna (bassi) 1875. 19705527572
selected clinicopathologic changes associated with experimentally induced fascioloides magna infection in white-tailed deer.six white-tailed deer (odocoileus virginianus), less than one year of age, were divided into two groups of three each and administered 50, or 500 metacercariae of ascioloides magna. all six deer became infected. three additional deer of the same age were uninoculated controls. all deer were monitored for up to 43 weeks after inoculation to investigate changes in weight, selected hematologic values, and blood chemistry values. although clinical disease was not evident in the infected deer, a sign ...1979459050
a natural infection of fascioloides magna in a llama (lama glama).a young, female llama (lama glama) was euthanized following the onset of hindleg paresis and paralysis. live trematodes, identified as fascioloides magna, were identified from the liver of this animal. this represents the first report of f. magna in a llama.19883357126
diseases of wapiti utilizing cattle range in southwestern alberta.specimens from 28 wapiti (cervus elaphus canadensis) were collected by hunters in southwestern alberta in 1984. various tests were performed to detect infections and conditions that could affect cattle sharing the range or cause disease in wapiti. serum antibodies were present against leptospiral serovars autumnalis (25%), bratislava (4%), and icterohaemorrhagiae (8%), and the viruses of bovine virus diarrhea (52%), infectious bovine rhinotracheitis (45%), and parainfluenza type 3 (13%). no sero ...19873029443
efficacy of triclabendazole against experimentally induced fascioloides magna infections in sheep.efficacy of oral administration of 20 mg of triclabendazole/kg of body weight was evaluated against 12-week fascioloides magna infections in 12 sheep, each inoculated orally with 250 viable metacercariae. from 6 sheep treated with triclabendazole, 1 immature f magna was recovered, whereas 116 f magna with a mean length of 19 +/- 6.5 mm were recovered from 6 untreated control sheep. efficacy of triclabendazole was 99.14%. signs of toxicosis or illness were not observed in the sheep.19892930033
efficacy of triclabendazole against fascioloidiasis (fascioloides magna) in naturally infected white-tailed deer (odocoileus virginianus).the efficacy of triclabendazole was evaluated in the treatment of naturally acquired fascioloides magna infections in white-tailed deer (odocoileus virginianus). twenty white-tailed deer were captured on the welder wildlife refuge (sinton, san patricio county, texas, usa) and maintained in a 64 x 64 m deer enclosure. ten deer were given a 5% suspension of triclabendazole orally at a dosage of 10 mg/kg body weight and 10 deer were given a placebo. three wk later the deer were euthanized and exami ...19892761011
restriction enzyme mapping of ribosomal dna can distinguish between fasciolid (liver fluke) species.recognition sites for nine different restriction endonucleases were mapped on rdna genes of fasciolid species. southern blots of digested dna from individual worms were probed sequentially with three different probes derived from rdna of schistosoma mansoni and known to span between them the entire rdna repeat unit in that species. eighteen recognition sites were mapped for fasciola hepatica, and seventeen for fasciola gigantica and fascioloides magna. each fasciolid species had no more than two ...19892552311
fascioloides magna in cattle. 19827082230
integrated control of fascioloides magna infection in northern italy.the following study records ten years' experience in the control of fascioloides magna infection in a fenced area grazed by an overdense red deer population together with free-ranging cattle. during the winter 1977/78 mortality due to a "fascioloidosis-malnutrition syndrome" reduced the deer stock almost by half. control measures were aimed at combatting the fluke in the wild definitive host by the administration of medicated pellets. four flukicides were used over the years: rafoxanide, diamphe ...19892486993
miracidial penetration in fascioloides magna (trematoda).the penetration of the miracidium of fascioloides magna into the snail host fossaria bulimoides is followed by examining the process at certain, timed intervals during the course of penetration. the cilia are lost early, but the epithelial cells may detach or they may be carried into the snail body. the apical gland releases vesicles which lose their outer coat in the snail tissue; similarly the vesiculated gland cells release vesicles which lose their coating in the snail tissue where there is ...19817314811
preliminary report: immunodiagnosis of pre-type ii ostertagiasis.ostertagia ostertagi soluble antigens were prepared by gel electrophoresis and electrophoretic transfer onto nitrocellulose for enzyme-linked immunosorbent assays with serum probes. serologic responses to l3-derived antigen of approximately 32 kda may be unique and diagnostic for cattle harboring inhibited larvae, or pre-type ii ostertagiasis. specificity was evaluated by comparing sera from pre-type ii cattle to sera from type i, uninfected, fascioloides magna infected, fasciola hepatica infect ...19883363842
efficacy of clorsulon against fascioloides magna infection in sheep.in a study to evaluate the efficacy of clorsulon against fascioloides magna infection in sheep, 12 ewes were inoculated orally with 100 metacercariae of f magna, and 6 were treated with clorsulon (15 mg/kg of body weight) 8 weeks after inoculation. the sheep were euthanatized 16 weeks after inoculation, flukes were recovered, and the liver and other tissues were subjectively scored for the severity of lesions (0 to 4+). the number of flukes recovered from the clorsulon-treated group (3.8 +/- 1.2 ...19883366676
evaluation of clorsulon against immature fascioloides magna in cattle and sheep.efficacy of clorsulon was evaluated against infection with immature fascioloides magna in 24 cattle and 12 sheep. infections were induced by oral administration of 600 metacercariae/host. in cattle, clorsulon at dosages of 7 and 21 mg/kg of body weight was 65 and 100% effective against 8-week-old flukes, and 20 and 74% effective against 16-week-old flukes, respectively. in sheep, clorsulon at a dosage of 21 mg/kg was 92% effective against 8-week-old flukes. significantly (p less than 0.05) more ...19883421522
prevalence of fascioloides magna in cattle and deer in michigan.a survey among 1.12 million cattle slaughtered in 357 packing plants in michigan during 1977 to 1981 was conducted to determine the prevalence of liver fluke infection. the condemnation rate of liver fluke-infected livers was 0.41, 3.7, and 13.9% in the southern, northern-lower, and upper regions of michigan, respectively. the same trend in infection rates was observed in white-tailed deer that had been examined in diagnostic laboratories in the state. a serologic survey among 50 randomly select ...19873667410
scanning electron microscopy and chemical excystation of fascioloides magna (trematoda) metacercariae.scanning electron microscopy was used to study encysted metacercariae and newly excysted juveniles of fascioloides magna. the outer cyst was rough, coarse and discontinuous in the ventral aspect; the inner cyst was smooth. the newly excysted metacercaria was plump and contained numerous tegumentary spines; large dome-shaped papillae were prominent around the oral sucker and on the rim of the acetabulum. encysted metacercariae with outer cysts were excysted in an alkaline bile salts-trypsin mediu ...19863776316
in vitro excystment of metacercariae of fascioloides magna (trematoda). 19853998966
activity of closantel against experimentally induced fascioloides magna infection in sheep.the efficacy of closantel against experimentally induced fascioloides magna infection in sheep was studied. in each of 3 experiments, closantel was administered 8 weeks after the sheep were given (oral inoculation) 100 metacercariae of f magna. in the 1st experiment, closantel was given orally to 5 groups of 6 sheep each at dosages of 0 (nontreated control), 5, 7.5, 10, and 15 mg/kg of body weight. in the 2nd and 3rd experiments, groups of 10 or 12 sheep were treated to confirm the efficacy of t ...19854083587
letter: lymnaea tomentosa from australia, an experimental intermediate host of the large american liver fluke, fascioloides magna. 19744447532
ovulation of the trematode fascioloides magna in relation to the duration of parasitation. 19724677617
galba modicella and lymnaea stagnalis as experimental intermediate hosts for fascioloides magna in minnesota. 19734687484
action of oxyclozanide against adult fascioloides magna (bassi, 1875) infections in white-tailed deer. 19734687498
parelaphostrongylus tenuis (nematoda) and fascioloides magna (trematoda) in moose of southeastern manitoba. 19744817542
efficacy of rafoxanide and oxyclozanide against fascioloides magna in naturally infected cattle. 19744819720
further observations on the survival of metacercariae of fascioloides magna in water at room temperature and under refrigeration. 19744821120
survival of metacercariae of fascioloides magna in water at room temperature and under refrigeration. 19725022879
the efficacy of closantel against fascioloides magna in sheep. 19846491851
efficacy of albendazole against fascioloides magna in sheep.sheep experimentally infected with fascioloides magna were used in a controlled study to determine the flukicide activity of different doses of albendazole (methyl [5(propylthio)-1h-benzimidazol-2-yl] carbamate). sheep, about 4 months old, were inoculated with 100 metacercariae of f magna and treated with the various doses of albendazole 10 weeks later. at 6 weeks after treatment, the sheep were necropsied, flukes were recovered and measured, and the pathologic changes were recorded. the sheep w ...19846703459
[the prevention and therapy of fascioloidiasis using bithionol sulfoxide].the elimination of the sources of invasion, i. e. hay and forage coming from the infested biotopes, combined with the administration of bithionol sulphoxide, resulted in a reduction of the invasion of cattle by fascioloides magna from 21.1 to 3.2% within two years. the czechoslovak-produced bithionol sulphoxide, administered at a rate of 40 to 50 mg per kg body weight as medicated feed, showed 100% effectiveness. at doses higher than 30 mg per kg body weight, the protective period should be prol ...19806777958
albendazole treatment of experimentally induced fascioloides magna infection in goats. 19806904122
[diffusion of helminth parasites of the digestive system and respiratory system in deer (cervus elaphus) of the la mandria regional park (piedmont)].a study was carried out on the diffusion of helminth parasites of liver, gastrointestinal system and bronchial system in 68 red deer (cervus elaphus) from regional park "la mandria" (piedmont, italy). the following species of nematodes were identified: trichostrongylus axei, spiculopteragia spiculoptera, rinadia mathevossiani, cooperia oncophora, cooperia pectinata, cooperia punctata, cooperia zurnabada, oesophagostomum venulosum, dictyocaulus eckerti, varestrongylus sagittatus. studies on some ...19807312394
[the influence of habitat on the occurrence of parasites in game animals].we studied the incidence of parasites of game animals under conditions of the individual habitats occupied by game animals. our investigations were made both in the open field and in game reserves for several game species, and also in biotopes where game animals come into contact with domestic ruminants. we detected 72 nematode species in a total of 8 species of game animals. the results show that, the composition of the helminth fauna is influenced by conditions of the external environment, i. ...19807436036
experimental infection of domestic goats with fascioloides magna.development of the deer liver fluke, fascioloides magna, was studied in seven domestic goats, each experimentally inoculated with 250 metacercariae. six infected goats died between 89 and 195 days (mean 139) after inoculation. infection did not develop in the seventh goat. mortality was the result of unrestricted fluke migration before maturation or encapsulation of the flukes. a total of 144 f magna (mean intensity of 24.0) were recovered from inoculated goats, representing 8.2% of the metacerc ...19807436074
further study on lymnaea stagnalis (l.) as a snail host for fascioloides magna (bassi, 1875) (trematoda). 195413131189
studies on larval fascioloides magna. i. observations on the survival of rediae in vitro. 196113701973
studies on larval fascioloides magna. ii. in vitro survival of axenic rediae in amino acids and sugars. 196113701974
fascioloides magna. 19807444593
clinical fascioloides magna infection in sheep in oregon on pasture shared by columbian white-tailed deer. 19807447146
pathogenicity of immature fascioloides magna in white-tailed deer.the pathogenesis of early prepatent fascioloides magna infection was investigated in seven fawns (odocoileus virginianus) given 500 metacercariae and examined at one, two, three, five, eight, 12 and 13 weeks postinoculation. blood samples were taken from eight inoculated deer every two weeks up to 16 weeks postinoculation. eosinophilia with a mild transitory anemia were the main clincopathological features. postmortem examination at two weeks postinoculation revealed extensive migration of immat ...19807448629
detection of fasciola hepatica in infected intermediate hosts using rt-pcr.fasciola hepatica, the common bile duct fluke, is an economically important parasite of domestic livestock. current research interest is directed toward an understanding of the parasite's biology at the intermediate host level. to permit study of seasonal transmission patterns and parasite/intermediate host interactions, a fasciolid-specific assay has been developed to detect infected snail vectors. this assay uses the reverse transcriptase-polymerase chain reaction (rt-pcr) to amplify specifica ...19947523650
a repetitive dna probe for the sensitive detection of fasciola hepatica infected snails.epizootiologic studies on f. hepatica frequently use microscopic techniques for the detection of infected snails, however, the poor efficiency, sensitivity, and specificity associated with these techniques limit their usefulness. a dna-based test for the identification of snails infected with larval stages of f. hepatica would solve these problems and enable a level of detection accuracy previously unavailable. we have cloned and sequenced a 124 bp fragment of repetitive dna from f. hepatica whi ...19957635638
use of bait containing triclabendazole to treat fascioloides magna infections in free ranging white-tailed deer.triclabendazole-medicated corn bait was given to white-tailed deer (odocoileus virginianus) on the welder wildlife refuge, sinton, texas (usa), at a dose of 11 mg/kg body weight per deer per day for seven days, for control of fascioloides magna. medicated bait was offered for one week each during the winters of 1987, 1988, and 1989. deer collected from treated areas, from baited control and from unbaited control areas were examined before the start of the study in 1987, and four weeks after the ...19947933276
an abattoir study of tuberculosis in a herd of farmed elk.the purpose of this study was to examine the prevalence and distribution of grossly visible lesions of tuberculosis in a herd of 344 north american elk (cervus elaphus) depopulated during a three-month period in 1991. abattoir inspection detected mycobacterial lesions in 134 (39.8%) of the 337 animals received for slaughter. the prevalence of lesions increased with the age of the animals. lesions were predominantly suppurative rather than caseous, and mineralization was less evident than in tube ...19947954222
comparison of the second internal transcribed spacer (ribosomal dna) from populations and species of fasciolidae (digenea).the nucleotide sequence of the 3' end of the second internal transcribed spacer region (its2) was determined for four isolates of fasciola hepatica, two isolates of fasciola gigantica, one isolate of fascioloides magna and one isolate of fasciola sp. from japan. intraspecific sequence divergence was negligible. sequence divergence between f. hepatica and f. gigantica was 2.8% and between fasciola hepatica and fascioloides magna was 13.2%. the sequence of fasciola sp. from japan matched closely t ...19938359995
enzyme-linked immunoelectrotransfer blot analysis of excretory-secretory proteins of fascioloides magna and fasciola hepatica.fasciola hepatica is a parasite of cattle (bos taurus), but not of white-tailed deer (odocoileus virginianus), while fascioloides magna is a parasite of white-tailed deer which also infects cattle as dead-end host. adult parasites were collected from naturally infected white-tailed deer or cattle. excretory-secretory proteins (esp) were obtained from each parasite. protein banding patterns were analysed on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and probed using sera ...19958533275
distribution of potential intermediate hosts for fasciola hepatica and fascioloides magna in montana, usa.a collection of lymnaeid snails in montana was made over a 3 year period, in conjunction with a state-wide survey of the distribution of liver flukes in montana. collection areas were selected based on reports of infected cattle, sheep or wildlife, and with the intent of covering all geographic regions of the state. snails were found at all 97 of the locations chosen for collections, with lymnaeids collected at 71 of the locations. the 97 sites were located in 28 of montana's 56 counties. nine l ...19968638394
susceptibility of bighorn sheep (ovis canadensis) to experimentally-induced fascioloides magna infections.three captive rocky mountain bighorn sheep (ovis canadensis canadensis), consisting of a female lamb, a yearling ram, and a 2 1/2-yr-old castrated ram were inoculated orally with 50 (n = 1) or 100 (n = 2) metacercariae of fascioloides magna in november 1991. all three sheep died from fluke infection on post-inoculation days 104, 140, and 197, respectively. numbers of f. magna recovered were 3 (3%), 18 (36%), and 21 (21%). all dukes were immature and were recovered from liver (n = 36), lungs (n = ...19968827689
the occurrence of fascioloides magna and fasciola hepatica together in the livers of naturally infected cattle in south texas, and the incidence of the flukes in cattle, white-tailed deer, and feral hogs. 19725078583
fascioloides magna in the pig--3 cases. 19715106718
epizootiologic factors of fascioloides magna (trematoda) in oregon and southern washington. 19676068250
efficacy of triclabendazole against fasciola hepatica and fascioloides magna in naturally infected calves.twenty calves (8 to 12 months old), naturally infected with fasciola hepatica and fascioloides magna, were obtained from brazoria, tex. they were randomly allotted to 3 treatment groups. one group (6 calves) was given the carrier without drug, the 2nd group (7 calves) was treated with triclabendazole at 6 mg/kg of body weight, and the 3rd group (7 calves) was treated with triclabendazole at 12 mg/kg. all treatments were administered as a drench. calves given triclabendazole (either dosage level) ...19846476577
the prevalence of fasciola hepatica in its snail intermediate host determined by dna probe assay.accurate snail intermediate host infection prevalence data have the potential to be extremely useful in determining seasonal transmission dynamics of fasciola hepatica. because the microscopic techniques currently used lack the sensitivity and specificity necessary to obtain meaningful infection prevalence data, we developed a highly accurate and efficient dna probe assay. the assay has a sensitivity of 100%, a specificity of > 99%, easily detects a single miracidia and does not cross-hybridize ...19979467746
a new intermediate host for fascioloides magna (bassi, 1873) ward, 1917. 193317758298
biomphalaria glabrata embryonic (bge) cell line supports in vitro miracidial transformation and early larval development of the deer liver fluke, fascioloides magna.a bge cell co-culture system, previously shown to support the in vitro production of daughter sporocysts from mother sporocysts of schistosoma mansoni and s. japonicum, has proven capable of supporting the in vitro development of intramolluscan stages of the deer liver fluke, fascioloides magna. miracidia commenced transforming within 4 h of incubation with bge cells, and had completely shed their epidermal plates within 18-24 h. redial stages were visible inside in vitro-transformed mother spor ...199910028533
survey of fascioloides magna in farmed wapiti in alberta.the formalin-ethyl acetate sedimentation procedure was used to detect ova of the giant liver fluke, fascioloides magna, in feces of farmed wapiti in alberta. twenty (3.2%) of the 629 fecal samples examined contained ova of f. magna. thirteen (33.3%) of the 39 farms surveyed had wapiti positive for f. magna. the presence of f. magna in farmed wapiti north of the north saskatchewan river is confirmed, and 3 areas where the infection has become endemic are identified.199910200881
flukes without snails: advances in the in vitro cultivation of intramolluscan stages of trematodes.in vitro cultivation of parasitic helminths, including the digenetic trematodes, has long been a valuable tool in medical and veterinary parasitology, permitting and/or facilitating the development of diagnostic reagents, chemotherapeutic agents, and vaccines and providing insights into naturally complex host-parasite interactions. in vitro cultivation of the intramolluscan stages of trematodes has been particularly challenging, given the ontogenic complexities involved in the production of mult ...200010631085
a natural focus of the blood fluke orientobilharzia turkestanica (skrjabin, 1913) (trematoda: schistosomatidae) in red deer (cervus elaphus) in hungary.the large american liver fluke, fascioloides magna - introduced to europe before 1875 - was the first non-indigenous trematode to be detected in hungarian cervids in 1995. as the most precious deer population became infected in the gemenc game reserve a study has been launched to assess the extent and the effect of f. magna infections and to examine the options for the treatment of infected deer populations. livers of red deer shot during regular hunting were submitted for the survey and were in ...201020347227
health protocol for translocation of free-ranging elk.when considering an elk (cervus elaphus) restoration program, wildlife managers must evaluate the positive and negative elements of translocation. we prepared this protocol to give an overview of health considerations associated with translocation of elk, with an emphasis on movement of free-ranging elk from western north america to the southeastern usa. we evaluated infectious agents and ectoparasites reported in elk from two perspectives. first, we made a qualitative estimate of the ability of ...200111504216
analysis of serum and whole blood values in relation to helminth and ectoparasite infections of feral pigs in texas.in the summers of 1996 and 1997, 60 wild pigs (sus scrofa) were necropsied from three sites in south texas (usa) to test the hypothesis that serum and whole blood parameters vary significantly (p < or = 0.05) with the prevalence and intensity of parasites infecting wild pigs. we found ten parasite species: five nematodes (metastrongylus salmi, metastrongylus pudentotectus, stephanurus dentatus, oesophagostomum dentatum, and physocephalus sexalatus); four ixodid ticks (amblyomma cajennense, ambly ...200212038138
studies on larval fascioloides magna. iii. mass hatching of miracidia by exposure to nitrogen. 196113895221
a comparative study of karyotypes and chromosomal location of rdna genes in important liver flukes fasciola hepatica and fascioloides magna (trematoda: fasciolidae).chromosomal characteristics, i.e., number, size, morphology, and location of ribosomal dna (rdna) clusters were examined in two medically important liver flukes, fasciola hepatica and fascioloides magna (fasciolidae), using conventional giemsa staining and fluorescent in situ hybridization (fish) with ribosomal 18s rdna probe. a comparison of f. magna and f. hepatica karyotypes confirmed significant differences in all chromosomal features. whilst the karyotype of f. hepatica comprised ten pairs ...201121509448
studies on larval fascioloides magna. iv. chromatographic analyses of free amino acids in the hemolymph of a host snail. 196113895222
natural infection of a horse with fascioloides magna.a 25-year-old quarterhorse mare was euthanized for a variety of medical reasons. at necropsy, 7 liver flukes, identified as fascioloides magna, were recovered from the liver. this is the first report of f. magna in a horse.200516131000
monitoring and treatment of fascioloides magna in semi-farm red deer husbandry in croatia.the presence of american liver fluke (fascioloides magna) in croatian wild ruminant species was detected for the first time in january 2000. at the same time, the problem of adequate parasitological monitoring and treatment appeared in the captive deer population. quarantine and health screening protocols, as well as migration and transportation influence had to be evaluated in red deer husbandry. non-invasive methods were introduced to estimate the prevalence of f. magna in the semi-farm rearin ...200516244930
first studies on the susceptibility of omphiscola glabra (gastropoda: lymnaeidae) from central france to fascioloides magna.experimental infections of omphiscola glabra (preadult snails), originating from central france, to a czech isolate of fascioloides magna miracidia were carried out to determine if the local populations of o. glabra may ensure the larval development of this parasite and to compare these results with those noted for a natural snail host, galba truncatula. the presence of experimentally infected snails was noted in the six populations of snails studied. however, only a few snails shed their cercar ...200616362339
cercarial production of fascioloides magna in the snail galba truncatula (gastropoda: lymnaeidae).single-miracidium infections of fascioloides magna in two populations of galba truncatula were carried out under laboratory conditions to count free rediae and cercariae in snail cadavers just after death. cercaria-shedding snails were in low numbers, and their shell height at day 60 p.e. was significantly greater than that of numerous infected snails that died without cercarial shedding. in snails that died between days 44 and 60 p.e. (at 20 degrees c), the numbers of second-generation rediae s ...200616416118
effects of six fasciolicides against fascioloides magna in white-tailed deer.thirty-three white-tailed deer (odocoileus virginianus) of various ages, both sexes, and in good physical condition were captured for anthelmintic evaluation of six compounds against the large american liver fluke, fascioloides magna. based on fluke mortality, hexachlorophene administered at the rate of 12 to 26 mg/kg of body weight was lethal to 5 of 10 mature flukes in seven deer. nitroxynil at 11 to 24 mg/kg inhibited egg production, but did not kill mature flukes in eight deer. rafoxanide at ...197616498878
parasites of the collared peccary from texas.results of a survey of the parasites of the collared peccary (dicotyles tajacu angulatus) in texas are presented. three ectoparasites, amblyomma cajennense, dermacentor variabilis, and pulex porcinus were very common on peccaries from south texas, but less common or absent in arid west texas. sucking lice, pecaroecus javalii, were common on peccaries from west texas, but were not found in south texas. the known range of this louse in texas is extended into the big bend area. two ticks, amnblyomm ...197016509125
phylogenetic data suggest the reclassification of fasciola jacksoni (digenea: fasciolidae) as fascioloides jacksoni comb. nov.fasciola jacksoni (cobbold, 1869) is a highly prevalent (18-62%) species colonizing the liver (less frequently the lungs, kidneys, pericardia, and intestines) of elephas maximus indicus and elephas maximus maximus in the indomalayan region, causing cirrhosis, hemorrhages, and connective tissue proliferation. the phylogenetic relationships of fasciola jacksoni in relation to representative species of the superfamily echinostomatoidea was assessed using four independent dna regions. the analysis i ...201323411741
[incidence and control of the american giant liver fluke, fascioloides magna, in a population of wild ungulates in the danubian wetlands east of vienna].fascioloides magna,the giant liver fluke, is an introduced parasite imported to europe with infected game which is endemic in the austrian wetlands of the danube from vienna to the slovakian border. due to its pathogenicity (especially in roe deer) and its potential of transmission to domestic ruminants a project on the epidemiology and control of this parasite was carried out between 2000 and 2005. to assess distribution faecal droppings from red deer were collected in an area along the danube ...200617009716
the giant liver fluke fascioloides magna (bassi 1875) in cervids in the czech republic and potential of its spreading to germany.the giant liver fluke fascioloides magna is an important parasite of cervids in europe. from september 2003 to december 2005, faecal samples and livers of red deer (cervus elaphus) and fallow deer (dama dama) were investigated to determine the current distribution of the fluke in the czech republic. faecal samples were collected from 20 different areas, and livers of hunted deer were dissected from each locality to confirm f. magna infection. the prevalence of f. magna in examined areas determin ...200717016724
optimization of metacercarial production for three digenean species by the use of petri dishes for raising lettuce-fed galba truncatula.experimental infections of galba truncatula with fasciola hepatica, fascioloides magna, or paramphistomum daubneyi were carried out at 20 degrees c to determine if the use of 14-cm petri dishes for breeding lettuce-fed snails enhanced the characteristics of snail infections. compared to infected snails raised in boxes up to day 30 post-exposure and later in individual 35-mm dishes, the survival of g. truncatula kept in 14-cm dishes and the shell height of cercariae-shedding snails during the fir ...200717061111
identification of the boudicca and sinbad retrotransposons in the genome of the human blood fluke schistosoma haematobium.schistosomes have a comparatively large genome, estimated for schistosoma mansoni to be about 270 megabase pairs (haploid genome). recent findings have shown that mobile genetic elements constitute significant proportions of the genomes of s. mansoni and s. japonicum. much less information is available on the genome of the third major human schistosome, s. haematobium. in order to investigate the possible evolutionary origins of the s. mansoni long terminal repeat retrotransposons boudicca and s ...200617072464
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