| extracellular novel metalloprotease from xenorhabdus indica and its potential as an insecticidal agent. | proteases produced by xenorhabdus are known to play a significant role in virulence leading to insect mortality. the present study was undertaken to purify and characterize protease from xenorhabdus indica, an endosymbiont of nematode steinernema thermophilum, and to decipher its role in insect mortality and its efficacy to control helicoverpa armigera. a set of 10 strains of xenorhabdus isolated from different regions of india were screened for protease activity on the basis of zone of clearing ... | 2013 | 23928843 |
| providencia vermicola sp. nov., isolated from infective juveniles of the entomopathogenic nematode steinernema thermophilum. | in the course of isolating bacteria from infective juveniles of the entomopathogenic nematode steinernema thermophilum ganguly & singh, 2000, three isolates were obtained (op1t, op29 and vs3). on the basis of 16s rrna gene sequence analysis and riboprint patterns, these three strains were identical to each other but distinct from the type strains of the five recognized species of the genus providencia. based on biochemical and genomic analysis and supported by the low (<35 %) dna-dna relatedness ... | 2006 | 16514040 |
| biocontrol potential of steinernema thermophilum and its symbiont xenorhabdus indica against lepidopteran pests: virulence to egg and larval stages. | under laboratory conditions, the biocontrol potential of steinernema thermophilum was tested against eggs and larval stages of two important lepidopteran insect pests, helicoverpa armigera and spodoptera litura (polyphagous pests), as well as galleria mellonella (used as a model host). in terms of host susceptibility of lepidopteran larvae to s. thermophilum, based on the lc50 36 hr after treatment, g. mellonella (lc50 = 16.28 ij/larva) was found to be more susceptible than s. litura (lc50 = 85 ... | 2014 | 24643472 |
| statistical optimization of media components for production of fibrinolytic alkaline metalloproteases from xenorhabdus indica kb-3. | xenorhabdus indica kb-3, a well-known protease producer, was isolated from its entomopathogenic nematode symbiont steinernema thermophilum. since medium constituents are critical to the protease production, the chemical components of the selected medium (soya casein digest broth) were optimized by rotatable central composite design (rccd) using response surface methodology (rsm). the effects of all five chemical components (considered as independent variables), namely tryptone, soya peptone, dex ... | 2014 | 24864214 |
| evaluation of the efficacy of three indigenous strains of entomopathogenic nematodes from meghalaya, india against mustard sawfly, athalia lugens proxima klug (hymenoptera: tenthredinidae). | the objective of this study was to evaluate the efficacy of three indigenous strains of entomopathogenic nematodes (epn) from meghalaya, india, namely heterorhabditis indica poinar, karunakar and david, steinernema thermophilum ganguly and singh, and steinernema glaseri (steiner) against the last instar larva of mustard sawfly, athalia lugens proxima klug, a serious pest of mustard and radish in india. the larvae of a. lugens proxima were exposed to 10, 25, 50, 75 and 100 infective juveniles (ij ... | 2012 | 24082523 |
| effects of storage temperature on survival and infectivity of three indigenous entomopathogenic nematodes strains (steinernematidae and heterorhabditidae) from meghalaya, india. | three locally isolated strains of entomopathogenic nematodes (epns), viz. heterorhabditis indica, steinernema thermophilum and steinernema glaseri, from meghalaya, india were characterized in terms of storage temperature and survival and infectivity of their infective juveniles (ijs). the survival and infectivity of nematode ijs was studied at, 5 ± 2 and 25 ± 2 °c, for a period of 120 days, using deionized water as storage medium. the viability of nematode ijs was checked by mobility criterion a ... | 2016 | 27876904 |
| leucobacter iarius sp. nov., in the family microbacteriaceae. | a novel gram-positive bacterium, strain 40(t), was isolated in the course of identifying bacteria from infective juveniles of the entomopathogenic nematode steinernema thermophilum. based on 16s rrna gene analysis, strain 40(t) was found to be related to the type strains of recognized species of the genus leucobacter, family microbacteriaceae. the 16s rrna gene sequence similarity values of strain 40(t) and leucobacter albus iam 14851(t), leucobacter luti lmg 23118(t), leucobacter alluvii lmg 23 ... | 2007 | 17392186 |
| a novel species of xenorhabdus, family enterobacteriaceae: xenorhabdus indica sp. nov., symbiotically associated with entomopathogenic nematode steinernema thermophilum ganguly and singh, 2000. | in the search for novel xenorhabdus strains in a recently described nematode species, steinernema thermophilum, three strains (strain 28(t) = dsm 17382(t), strain 42 = dsm 17383 and strain 43 = dsm 17384) were isolated from three independent isolation approaches from crushed mixture of infective juveniles. 16s rrna gene sequence comparison of strains 28(t) and dsm 17383 indicated identity and the phylogenetic position pointed towards an individual taxon within the phylogenetic dendrogram of xeno ... | 2006 | 16459045 |
| soil moisture effects on the activity of three entomopathogenic nematodes (steinernematidae and heterorhabditidae) isolated from meghalaya, india. | entomopathogenic nematodes (epns) are obligate parasites of insects that are widely distributed in soils throughout the world. they have great potential for use as biological control agents for insect pests. it is known that strains of steinernema and heterorhabditis isolated from different geographical regions exhibit differences in their ecological traits, such as infectivity, establishment, survival, reproduction, etc. a precise knowledge of these factors is therefore an essential pre-requisi ... | 2011 | 23543771 |
| efficacy of indigenous entomopathogenic nematodes from meghalaya, india against the larvae of taro leaf beetle, aplosonyx chalybaeus (hope). | the efficacy of three entomopathogenic nematode (epn) species, heterorhabditis indica, steinernema thermophilum, and s. glaseri, from meghalaya, india was studied against the larvae of taro leaf beetle, aplosonyx chalybaeus (hope) (coleoptera: chrysomelidae), under the laboratory conditions. the beetle larvae (grubs) were exposed to 25, 50, 75, 100 and 200 infective juveniles (ijs) of each nematode species for different time periods and they were found to be susceptible to all the epns tested. h ... | 2012 | 24082518 |