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genetic diversity and phylogeography of cassava mosaic viruses in kenya.cassava is a major factor in food security across sub-saharan africa. however, the crop is susceptible to losses due to biotic stresses, in particular to viruses of the genus begomovirus (family geminiviridae) that cause cassava mosaic disease (cmd). during the 1990s, an epidemic of cmd severely hindered cassava production across eastern and central africa. a significant influence on the appearance of virus epidemics is virus diversity. here, a survey of the genetic diversity of cmd-associated b ...200616963765
east african cassava mosaic-like viruses from africa to indian ocean islands: molecular diversity, evolutionary history and geographical dissemination of a bipartite begomovirus.cassava (manihot esculenta) is a major food source for over 200 million sub-saharan africans. unfortunately, its cultivation is severely hampered by cassava mosaic disease (cmd). caused by a complex of bipartite cassava mosaic geminiviruses (cmg) species (family: geminivirideae; genus: begomovirus) cmd has been widely described throughout africa and it is apparent that cmg's are expanding their geographical distribution. determining where and when cmg movements have occurred could help curtail i ...201223186303
molecular characterization of a new alphasatellite associated with a cassava mosaic geminivirus in madagascar.two complete nucleotide sequences of an alphasatellite isolated from a cassava plant with mosaic disease symptoms in madagascar are described and analyzed. while the helper begomovirus was identified as an isolate of east african cassava mosaic kenya virus (eacmkv), its associated alphasatellite was most closely related (80 % nucleotide sequence identity) to cotton leaf curl gezira alphasatellite. these satellite molecules have typical features of alphasatellites, with a single gene in the virio ...201323525698
divergent evolutionary and epidemiological dynamics of cassava mosaic geminiviruses in madagascar.cassava mosaic disease (cmd) in madagascar is caused by a complex of at least six african cassava mosaic geminivirus (cmg) species. this provides a rare opportunity for a comparative study of the evolutionary and epidemiological dynamics of distinct pathogenic crop-infecting viral species that coexist within the same environment. the genetic and spatial structure of cmg populations in madagascar was studied and bayesian phylogeographic modelling was applied to infer the origins of madagascan cmg ...201627600545
infectivity, pseudorecombination and mutagenesis of kenyan cassava mosaic begomoviruses.cloned dna-a and dna-b components of kenyan isolates of east african cassava mosaic virus (eacmv, eacmv-ug and eacmv-ke2), east african cassava mosaic kenya virus (eacmkv) and east african cassava mosaic zanzibar virus (eacmzv) are shown to be infectious in cassava. eacmv and eacmkv genomic components have the same iteron sequence (ggggg) and can form viable pseudorecombinants, while eacmzv components have a different sequence (ggaga) and are incompatible with eacmv and eacmkv. mutagenesis of ea ...200717412996
diversity of begomoviruses associated with mosaic disease of cultivated cassava (manihot esculenta crantz) and its wild relative (manihot glaziovii mull. arg.) in uganda.cassava (manihot esculenta) growing in uganda during 2001-2002 has been screened for the presence of begomoviruses using pcr-rflp, cloning full-length genomic components and nucleotide sequence analysis. in contrast with a recent survey in neighbouring kenya, which identified three distinct strains of east african cassava mosaic virus (eacmv, eacmv-ug and eacmv-ke2) as well as east african cassava mosaic zanzibar virus and the new species east african cassava mosaic kenya virus, only eacmv-ug an ...200818559947
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