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metagenomic detection of viral pathogens in spanish honeybees: co-infection by aphid lethal paralysis, israel acute paralysis and lake sinai viruses.the situation in europe concerning honeybees has in recent years become increasingly aggravated with steady decline in populations and/or catastrophic winter losses. this has largely been attributed to the occurrence of a variety of known and "unknown", emerging novel diseases. previous studies have demonstrated that colonies often can harbour more than one pathogen, making identification of etiological agents with classical methods difficult. by employing an unbiased metagenomic approach, which ...201323460860
phylogenetic and serological analysis of turnip ringspot virus and radish mosaic virus isolates.turnip ringspot virus (tursv) has been proposed to be a member of a new species in the genus comovirus. its remarkable host-range similarity to radish mosaic virus (ramv) may have led to its misrecognition in the past. findings from both sequence analysis and serological tests support the assignment of tursv to a new comovirus species. in addition, phylogenetic analysis suggests that the two genome segments of some tursv isolates have a heterogeneous origin.201222160585
complete nucleotide sequence of the toledo isolate of turnip ringspot virus.the complete genomic sequence of the toledo isolate of the comovirus, turnip ringspot virus (tursv), was found to consist of 2 polyadenylated rnas. rna 1 is 6082 nucleotides long and encodes a single predicted polypeptide of 1860 amino acids. the predicted rna 1 polyprotein contains the polypeptides for viral replication and proteolytic processing. rna 2, that is 3985 nucleotides long, codes for a single predicted 1095 amino acid polypeptide containing the movement and coat proteins. phylogeneti ...200919838619
emerging viruses in the genus comovirus.the intraspecies variability of capsid proteins of five viruses of the genus comovirus was established. inclusion of both capsid proteins to the sequence analysis reduces some uncertainties about species/strains demarcation criteria in the comovirus genus. new approach is proposed for discrimination of turnip ringspot virus as a separate species.201020044790
complete genome sequence of turnip ringspot virus.here we present the complete genome sequences of two tursv isolates. they are 90-100% identical in distinct genes, but reasonably less identical with ramv isolates. regarding the cps, tursv and ramv have an aa sequence identity of 72-74% among all isolates and the proposed cut-off level is 75%. for the proteinase-polymerase region, the average value between the two isolates of tursv and three isolates of ramv is 79.8% and the cut-off level is 80%. at the moment, tursv and ramv are the two identi ...200919774335
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