| aquincola tertiaricarbonis gen. nov., sp. nov., a tertiary butyl moiety-degrading bacterium. | strains l10(t), l108 and cip i-2052 were originally obtained from methyl tert-butyl ether (mtbe)-contaminated groundwater and from a wastewater treatment plant, respectively. all share the ability to grow on tert-butanol, an intermediate of mtbe degradation. cells are strictly aerobic, motile by a polar flagellum and exhibit strong pili formation. poly beta-hydroxybutyrate (phb) granules are formed. the dna g+c content is 69-70.5 mol% and the main ubiquinone is q-8. the major cellular fatty acid ... | 2007 | 17551046 |
| degradation of fuel oxygenates and their main intermediates by aquincola tertiaricarbonis l108. | growth of aquincola tertiaricarbonis l108 on the fuel oxygenates methyl tert-butyl ether (mtbe), ethyl tert-butyl ether (etbe) and tert-amyl methyl ether (tame), as well as on their main metabolites tert-butyl alcohol (tba), tert-amyl alcohol (taa) and 2-hydroxyisobutyrate (2-hiba) was systematically investigated to characterize the range and rates of oxygenate degradation by this strain. the effective maximum growth rates for mtbe, etbe and tame at ph 7 and 30 degrees c were 0.045 h(-1), 0.06 h ... | 2008 | 18451050 |
| ideonella azotifigens sp. nov., an aerobic diazotroph of the betaproteobacteria isolated from grass rhizosphere soil, and emended description of the genus ideonella. | strain 1a22t, a nitrogen-fixing bacterium, was isolated from soil associated with the rhizosphere of a perennial grass growing in a fallow agricultural field in ithaca, new york, usa. analysis of the 16s rrna gene sequence placed the strain in the rubrivivax-roseateles-leptothrix-azohydromonas-aquincola-ideonella branch of the betaproteobacteria and the closest characterized relative was the type strain of ideonella dechloratans (97.7% 16s rrna sequence similarity). cells of strain 1a22t were gr ... | 2009 | 19567574 |
| evaluation of the effects of low oxygen concentration on stable isotope fractionation during aerobic mtbe biodegradation. | laboratory experiments were performed with two aerobic mtbe degrading strains ( methylibium sp. pm1 and aquincola tertiaricarbonaris l108) in order to determine whether conditions of low oxygen availability, typically found in fuel-contaminated aquifers, can influence stable isotope fractionation of mtbe. although single carbon and hydrogen enrichment factors of the two strains were not significantly or were only slightly (l108) affected by low oxygen concentrations (fully oxic incubation with i ... | 2010 | 19928956 |
| biosynthesis of 2-hydroxyisobutyric acid (2-hiba) from renewable carbon. | nowadays a growing demand for green chemicals and cleantech solutions is motivating the industry to strive for biobased building blocks. we have identified the tertiary carbon atom-containing 2-hydroxyisobutyric acid (2-hiba) as an interesting building block for polymer synthesis. starting from this carboxylic acid, practically all compounds possessing the isobutane structure are accessible by simple chemical conversions, e. g. the commodity methacrylic acid as well as isobutylene glycol and oxi ... | 2010 | 20184738 |
| formation of alkenes via degradation of tert-alkyl ethers and alcohols by aquincola tertiaricarbonis l108 and methylibium spp. | bacterial degradation pathways of fuel oxygenates such as methyl tert-butyl and tert-amyl methyl ether (mtbe and tame, respectively) have already been studied in some detail. however, many of the involved enzymes are still unknown, and possible side reactions have not yet been considered. in aquincola tertiaricarbonis l108, methylibium petroleiphilum pm1, and methylibium sp. strain r8, we have now detected volatile hydrocarbons as by-products of the degradation of the tert-alkyl ether metabolite ... | 2011 | 21742915 |
| study of an aquifer contaminated by ethyl tert-butyl ether (etbe): site characterization and on-site bioremediation. | ethyl tert-butyl ether (etbe) was detected at high concentration (300mgl(-1)) in the groundwater below a gas-station. no significant carbon neither hydrogen isotopic fractionation of etbe was detected along the plume. etbe and btex biodegradation capacities of the indigenous microflora pz1-etbe and of a culture (mc-ifp) composed of rhodococcus wratislaviensis ifp 2016, rhodococcus aetherivorans ifp 2017 and aquincola tertiaricarbonis ifp 2003 showed that etbe and btex degradation rates were in t ... | 2011 | 22177017 |
| bacterial degradation of tert-amyl alcohol proceeds via hemiterpene 2-methyl-3-buten-2-ol by employing the tertiary alcohol desaturase function of the rieske non-heme mononuclear iron oxygenase mdpj. | tertiary alcohols, such as tert-butyl and tert-amyl alcohol (tba and taa, respectively) and higher homologues, are only slowly degraded microbially. conversion of tba seems to proceed via hydroxylation to 2-methylpropan-1,2-diol which is further oxidized to 2-hydroxyisobutyric acid. by analogy, a branched pathway is expected for the degradation of taa, as this molecule possesses several potential hydroxylation sites. in aquincola tertiaricarbonis l108 and methylibium petroleiphilum pm1, a likely ... | 2011 | 22194447 |