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seed transmission of beet curly top virus and beet curly top iran virus in a local cultivar of petunia in iran.beet curly top virus (bctv) and beet curly top iran virus (bctiv) are known as the causal agents of curly top disease in beet and several other dicotyledonous plants in iran. these viruses are transmitted by circulifer species, and until now, there has been no confirmed report of their seed transmission. a percentage (38.2-78.0%) of the seedlings developed from the seeds of a petunia local cultivar under insect-free conditions showed stunting, interveinal chlorosis, leaf curling, and vein swelli ...201729035342
genome sequences of beet curly top iran virus, oat dwarf virus, turnip curly top virus, and wheat dwarf virus identified in leafhoppers.implementation of a vector-enabled metagenomics approach resulted in the identification of various geminiviruses. we identified the genome sequences of beet curly top iran virus, turnip curly top viruses, oat dwarf viruses, the first from iran, and wheat dwarf virus from leafhoppers feeding on beet, parsley, pumpkin, and turnip plants.201728232449
genome characterization and genetic diversity of beet curly top iran virus: a geminivirus with a novel nonanucleotide.beet curly top iran virus (bctiv) was previously reported as a distinct curtovirus in iran. complete nucleotide sequences of three bctiv isolates, one each from central, southern, and south eastern iran were determined to be 2844, 2844, and 2845 nt long, respectively. bctiv shared highest nucleotide sequence identity (52.3%) with spinach curly top virus (spctv) and lowest identity (46.6%) with horseradish curly top virus (hrctv). the bctiv genome comprises three virion-sense (v1, v2, and v3) and ...200818401697
a highly divergent south african geminivirus species illuminates the ancient evolutionary history of this family.we have characterised a new highly divergent geminivirus species, eragrostis curvula streak virus (ecsv), found infecting a hardy perennial south african wild grass. ecsv represents a new genus-level geminivirus lineage, and has a mixture of features normally associated with other specific geminivirus genera.200919321000
fulfilling koch's postulates for beet curly top iran virus and proposal for consideration of new genus in the family geminiviridae.beet curly top iran virus (bctiv) is a divergent geminivirus with biological properties similar to those of curtoviruses; however, the virus is distinct from curtoviruses phylogenetically and in its genome organisation. the replication-associated protein is phylogenetically more closely related to those of mastreviruses than to those of curtoviruses whereas the capsid protein shares high amino acid sequence identity (77-83 %) with those of curtoviruses. the 17 bctiv genomes from iran share ~77 % ...201323081676
diversity of beet curly top iran virus isolated from different hosts in iran.beet curly top iran virus (bctiv) is a major pathogen of sugar beet in iran. in order to study diversity of bctiv, we sampled 68 plants in iran during the summer of 2010 with curly top disease symptoms on beans (phaseolus vulgaris), cowpeas (vigna unguiculata), tomatoes (solanum lycopersicum l.), sea beets (beta vulgaris subsp. maritima), and sugar beets (beta vulgaris). plant samples showing leaf curling, yellowing, and/or swelling of veins on the lower leaf surfaces were collected from various ...201323329008
intergeneric recombination between a new, spinach-infecting curtovirus and a new geminivirus belonging to the genus becurtovirus: first new world exemplar.a novel curtovirus, spinach severe curly top virus (ssctv), was associated with symptomatic spinach plants collected from a commercial field in south-central arizona during 2009. in addition, a second viral molecule of about 2.9 kb from the same spinach plants was amplified, cloned and sequenced. the latter isolate, herein named spinach curly top arizona virus (sctav), was found to share 77 % pairwise sequence identity with beet curly top iran virus (bctiv), a leafhopper-transmitted geminivirus ...201323708296
establishment of three new genera in the family geminiviridae: becurtovirus, eragrovirus and turncurtovirus.the family geminiviridae includes plant-infecting circular single-stranded dna viruses that have geminate particle morphology. members of this family infect both monocotyledonous and dicotyledonous plants and have a nearly global distribution. with the advent of new molecular tools and low-cost sequencing, there has been a significant increase in the discovery of new geminiviruses in various cultivated and non-cultivated plants. in this communication, we highlight the establishment of three new ...201424658781
splicing features in the expression of the complementary-sense genes of beet curly top iran virus.beet curly top iran virus (bctiv) is a distinct geminivirus which has been reported from sugar-beet-growing farms in iran. in this study, the role of the splicing in expression of complementary-sense genes of bctiv was studied. total rna was extracted from bctiv-infected tissue, and the predicted intron position of complementary-sense mrna transcripts was amplified by rt-pcr followed by cloning of the amplicons. sequence confirmed that both spliced and unspliced mrnas are synthesized by the same ...201728004232
molecular characterization and construction of an infectious clone of a pepper isolate of beet curly top iran virus.geminiviruses cause curly top disease, in dicotyledonous plants which constrains host crop production. beet curly top iran virus (bctiv) is a widespread becurtovirus (family geminiviridae) in numerous areas within iran. in this study, we isolated and analyzed a full-length genomic dna of a new variant of bctiv from pepper crops in the kaftark region, east of shiraz (proposed acronym: bctiv-kaf [ir: kaf:2016:pepper]). infected pepper plants showed shortening of internodes, severe interveinal chlo ...201628097164
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