| enzyme-linked immunosorbent assay using recombinant ospc and the internal 14-kda flagellin fragment for serodiagnosis of early lyme disease. | the outer surface protein c (ospc) and the internal 14-kda flagellin fragment of strain geho of borrelia burgdorferi sensu stricto were expressed as recombinant proteins in escherichia coli and were purified for use in an immunoglobulin m (igm) enzyme-linked immunosorbent assay (ospc-14-kda antigen elisa). no hint at disturbing protein-protein interferences, which might influence the availability of immunoreactive epitopes, was found when the recombinant antigens were combined in the elisa. the ... | 1998 | 9542898 |
| specificity of infection-induced immunity among borrelia burgdorferi sensu lato species. | the specificity of infection-induced immunity in mice infected with cultured or host-adapted borrelia burgdorferi sensu lato, the agent of lyme disease, was examined. sera obtained from mice following infection with high and low doses of cultured b. burgdorferi sensu stricto, transplantation of infected tissue (host-adapted spirochetes), or tick-borne inoculation all showed protective activity in passive immunization assays. infection and disease were similar in mice infected with cultured spiro ... | 1999 | 9864193 |
| osp17, a novel immunodominant outer surface protein of borrelia afzelii: recombinant expression in escherichia coli and its use as a diagnostic antigen for serodiagnosis of lyme borreliosis. | western blot analyses of the human humoral response of patients with lyme borreliosis have shown that a 17-kda protein is an immunodominant protein in late disease. immune electron microscopy with a monoclonal antibody against this protein revealed that the 17-kda protein is abundantly expressed on the surface of borrelia afzelii strain pko. therefore, the protein has been renamed outer surface protein (osp) 17. recombinant osp 17 of strain pko was expressed in escherichia coli and purified by c ... | 1999 | 10363678 |
| antibodies against specific proteins of and immobilizing activity against three strains of borrelia burgdorferi sensu lato can be found in symptomatic but not in infected asymptomatic dogs. | in an area where lyme disease is endemic in the netherlands all dogs had positive titers by whole-cell enzyme-linked immunosorbent assay and appeared to be naturally infected by borrelia burgdorferi sensu lato. to compare the antibody responses of symptomatic dogs and asymptomatic controls, we performed western blots and in vitro immobilization assays to study antibody-dependent bactericidal activity. strains from three different genospecies were employed as the antigen source: b. burgdorferi st ... | 2000 | 10878052 |
| development and laboratory evaluation of a new recombinant elisa for the serodiagnosis of lyme disease. | the use of recombinant proteins for serologic testing represents a modern approach for the improved laboratory diagnosis of lyme disease (ld). the aim of the present study was to develop and evaluate a new recombinant elisa (re) for the detection of specific igg and igm antibodies against borrelia burgdorferi. | 2002 | 12422605 |