| [the effect of 3',5'-cyclic adenylic acid (camp) on the growth of bacteria]. | the effect of intracellular camp level on the growth of bacteria was studied with e. coli as 1.797, corynebacterium pekinense as 1.299 and bacillus megaterium as 1.217. the experimental results show that the growth of e. coli as 1.797 was decreased with increasing camp level in cell. with glucose as the sole carbon source, intracellular camp is low, and growth of e. coli as 1.797 was inhibited by exogenous camp, but the analog metabolite of camp-5'-amp had no effect on growth. when e. coli as 1. ... | 1989 | 2552690 |
| [regulation of corynebacterium pekinense glutamine synthetase by the carbon and nitrogen source]. | | 1986 | 2883774 |
| fractionation of corynebacterium pekinense as 1.299 phage subtypes by anion-exchange chromatography. | a novel method to fractionate phage into its subtypes while fully retaining biological function is reported. corynebacterium pekinense as 1.299 phage samples, purified by either conventional ultracentrifugation or gel chromatography on a superose(r) 6 prep column (0.78 x 30 cm), were fractionated further into four fractions by anion-exchange chromatography on a toyopearl superq 650c column (0.5 x 20 cm) with a linear gradient of nacl concentration from 0.2 to 1.0 m in 0.02 m carbonate-biocarbona ... | 2001 | 11377709 |
| [cloning, sequence analysis and expression of anthranilate synthetase gene in corynebacterium pekinense]. | anthranilate synthetase (ec4.1.3.27;as) genes from wild-type corynebacterium pekinense as1.299 and its mutant pd-67 were cloned and sequenced. analysis of pcr fragments revealed that three orfs existed, which corresponded to trpl, trpe and trpg gene, respectively. six bases changes that resulted in the changes of five amino acids were found in the trpe structural gene of c. pekinense pd-67 and a single-base change that resulted in an amino acid substitution was found in the trpg structural gene ... | 2007 | 17436623 |
| [cloning, expression and sequence analysis of ds i gene in corynebacterium pekinense as1.299 and pd-67]. | 3-deoxy-d-arabinoheptulosonate-7-phosphate synthase (ec 2.5.1.54;ds) is the key enzyme in tryptophan synthesis pathway. cloning ds i gene from corynebacterium pekinense and expression of ds i gene might facilitate testing the existence and function of ds i in corynebacterium pekinense. | 2008 | 19149161 |
| [screening and breeding of high lysine-producing strains by genome shuffling]. | to screen and breed high lysine-producing strains by genome shuffling. | 2009 | 19835170 |
| [effect of phosphoenolpyruvate carboxylase gene knock-out on metabolism in corynebacterium pekinense pd-67]. | in order to optimize precursor supply for l-tryptophan biosynthesis, a corynebacterium pekinense pd-67 mutant with phosphoenolpyruvate carboxylase gene (ppc) in-frame deletion was constructed. the effect of ppc knock-out on physiological characteristics of the mutant was investigated. | 2010 | 21141467 |
| [corynebacterium pekinense transketolase: gene cloning, sequence analysis and expression]. | transketolase (ec 2. 2. 1. 1; tk) is the key enzyme in non-oxidative phosphate pentose pathway. we cloned tkt gene from corynebacterium pekinense as 1.299 and its mutant pd-67 in order to investigate the effect of gene expression on physiological characteristics of c. pekinense. pd-67. | 2010 | 21268892 |
| characterization of aspartate kinase from corynebacterium pekinense and the critical site of arg169. | aspartate kinase (ak) is the key enzyme in the biosynthesis of aspartate-derived amino acids. recombinant ak was efficiently purified and systematically characterized through analysis under optimal conditions combined with steady-state kinetics study. homogeneous ak was predicted as a decamer with a molecular weight of ~48 kda and a half-life of 4.5 h. the enzymatic activity was enhanced by ethanol and ni(2+). moreover, steady-state kinetic study confirmed that ak is an allosteric enzyme, and it ... | 2015 | 26633359 |
| [heterologous expression and characterization of l200f/d215k mutant of homoserine dehydrogenase from corynebacterium pekinense as1.299]. | to obtain a new homoserine dehydrogenase with better properties from corynebacterium pekinense by the spatial structure transfromation. | 2014 | 25803895 |
| [construction and characterization of r169h mutant of aspartokinase from corynebacterium pekinense]. | increasing the activity of aspartokinase (ak) from corynebacterium pekinense. | 2014 | 25272815 |
| [effect of pps and arogfbr overexpression on l-tryptophan production in corynebacterium pekinense]. | in order to redirect carbon flows into aromatic amino acids biosynthesis pathway and further improve the production of l-tryptophan in corynebacterium pekinense pd-67, two schemes were implemented. first, the supply of phosphoenolpyruvate (pep), one of precursors of l-tryptophan biosynthesis, was increased. second, the feedback inhibition of 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase (ds), a key enzyme in the aromatic amino acids biosynthesis, was relieved and the activity of ds was in ... | 2014 | 24783851 |
| [effect of aromatic amino acid transport gene knock-out on l-tryptophan accumulation in corynebacterium pekinense pd-67]. | a corynebacterium pekinense pd-67 mutant with aromatic amino acid transport system gene (arop) in-frame deletion was constructed to decrease the uptake of l-tryptophan and reduce the intracellular pool of l-tryptophan, further to deregulate the feedback regulation of l-tryptophan and increase the extracellular accumulation. the effects of arop knock-out as well as anthranilate synthetase (ec4. 1. 3. 27; as) gene overexpression on l-tryptophan accumulation of the mutant were investigated. | 2012 | 23383505 |