| high-throughput gender identification of accipitridae eagles with real-time pcr using taqman probes. | the objective was to develop high-throughput gender identification of eagles. based on blast and alignment analyses, the chd-z and chd-w sequences of nine species of eagles were highly homologous with spilornis cheela hoya (s. c. hoya); therefore, taqman probes were designed to target their chd-zw-common and chd-w-specific regions. in s. c. hoya, genders were identified using taqman-based, real-time pcr (amplified by p2/p8 primers); this method was validated with anatomically confirmed controls ... | 2008 | 18440628 |
| high-throughput avian molecular sexing by sybr green-based real-time pcr combined with melting curve analysis. | combination of chd (chromo-helicase-dna binding protein)-specific polymerase chain reaction (pcr) with electrophoresis (pcr/electrophoresis) is the most common avian molecular sexing technique but it is lab-intensive and gel-required. gender determination often fails when the difference in length between the pcr products of chd-z and chd-w genes is too short to be resolved. | 2008 | 18269737 |
| use of random amplified polymorphic dna to identify several novel markers for sex identification in the crested serpent eagle and crested goshawk. | the crested serpent eagle (spilornis cheela hoya) has no distinct sexual dimorphic traits. in the current study, we report the results of an ee0.6 (ecori 0.6-kb fragment) sequence applied to s. cheela hoya and a novel random amplified polymorphic dna (rapd) marker that can be used to sex individuals within the species s. cheela hoya and accipiter trivigatus formosae (crested goshawk). we used sex-specific primers for the avian chd1 (chromo-helicase-dna-binding 1) gene and the ee0.6 sequence in p ... | 2009 | 19560806 |
| validation of spilornis cheela hoya taqman probes for potential gender identification of many accipitridae species. | the objective of this study was to test the hypothesis that genders of accipitridae species, with the same or similar sequences to our previously proposed spilornis cheela hoya (s. c. hoya) chromo-helicase-dna binding protein (chd)-w-specific and chd-zw-common taqman probes, can be successfully determined. eight species of accipitridae with known genders were collected. after pcr, ta cloning, sequencing, and alignment analyses, sequence length differences of griffiths p2/p8 pcr amplicons between ... | 2010 | 19959218 |
| hematocrit and plasma chemistry values in adult collared scops owls (otus lettia) and crested serpent eagles (spilornis cheela hoya). | in this study, we report hematocrit and plasma chemistry values for adult captive collared scops owls (otus lettia) and crested serpent eagles (spilornis cheela hoya). in particular, we address the gender-specific differences within these values. we measured hematocrit (hct) and plasma chemistry values for uric acid (ua), plasma urea nitrogen (bun), total protein (tp), albumin (alb), glucose (glu), cholesterol (cho), triglyceride (tg), aspartate aminotransferase (ast), alanine aminotransferase ( ... | 2012 | 22446394 |
| anesthesia with isoflurane and sevoflurane in the crested serpent eagle (spilornis cheela hoya): minimum anesthetic concentration, physiological effects, hematocrit, plasma chemistry and behavioral effects. | the initial goal of this study was to determine the minimum anesthetic concentration (mac) for isoflurane (iso) and sevoflurane (sevo) for the crested serpent eagle. next, we compared the anesthetic effects of each on the physiological effects, hematocrit, plasma chemistry values and behavior in spontaneously breathing captive adult crested serpent eagles. sixteen eagles were randomly allocated to two groups for anesthesia with iso (n=8) or sevo (n=8). first, we measured the mac values of iso an ... | 2013 | 23955396 |
| an improved pcr method for gender identification of eagles. | eagles are sexually monomorphic and therefore it is difficult to determine their gender, which is a crucial need for management purposes. in this study, we have developed an improved gender identification method by exploiting length differences between the chromo-helicase-dna binding protein (chd)-z and chd-w genes of spilornis cheela hoya. by comparing dna sequences for chd-w and chd-z from 10 species of falconiformes eagles we designed universal gender identification pcr primers that exploit d ... | 2008 | 18385011 |