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halopiger xanaduensis gen. nov., sp. nov., an extremely halophilic archaeon isolated from saline lake shangmatala in inner mongolia, china.strain sh-6(t) was isolated from the sediment of lake shangmatala, a saline lake in inner mongolia (china). cells were pleomorphic. the organism was neutrophilic and required at least 2.5 m (15 %) nacl, but not mgcl(2), for growth; optimal growth occurred at 4.3 m (25 %) nacl. the g+c content of its dna was 63.1 mol%. 16s rrna gene sequence analysis revealed that strain sh-6(t) is a member of the family halobacteriaceae, but there was a low level of similarity with other members of this family. ...200717625165
halopiger aswanensis sp. nov., a polymer-producing and extremely halophilic archaeon isolated from hypersaline soil.strain 56(t) was isolated from a hypersaline soil in aswan (egypt). cells were pleomorphic rods. the organism was neutrophilic, motile and required at least 1.7 m (10 % w/v) nacl, but not mgcl(2), for growth; optimal growth occurred at > or =3.8 m (> or =22.5 %) nacl. the strain was thermotolerant with an optimum temperature for growth of 40 degrees c, although growth was possible up to 55 degrees c. the g+c content of the dna of the novel strain was 67.1 mol%. 16s rrna gene sequence analysis re ...201019654343
further refinement of the phylogeny of the halobacteriaceae based on the full-length rna polymerase subunit b' (rpob') gene.a considerable number of species of the halobacteriaceae possess multiple copies of the 16s rrna gene that exhibit more than 5 % divergence, complicating phylogenetic interpretations. two additional problems have been pointed out: (i) the genera haloterrigena and natrinema show a very close relationship, with some species being shown to overlap in phylogenetic trees reconstructed by the neighbour-joining method, and (ii) alkaliphilic and neutrophilic species of the genus natrialba form definitel ...201019946058
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