| moraxella bovoculi sp. nov., isolated from calves with infectious bovine keratoconjunctivitis. | eighteen isolates of a gram-negative coccus (strain 237(t)) were cultured from the eyes of dairy and beef calves affected with infectious bovine keratoconjunctivitis (ibk; 'pinkeye') in northern california, usa, during summer 2002. these isolates had near full-length (1397 bp) 16s rrna gene sequences that clustered into three groups with 99.9 % sequence similarity. on the basis of 16s rrna gene sequence, the isolates were most closely associated with moraxella bovis and moraxella ovis in clade i ... | 2007 | 17392208 |
| identification and characterization of complete rtx operons in moraxella bovoculi and moraxella ovis. | to determine if moraxella bovoculi (m. bovoculi), a recently characterized coccoid moraxella that was isolated from the eyes of calves affected with infectious bovine keratoconjunctivitis (ibk), and moraxella ovis (m. ovis), originally isolated from sheep with conjunctivitis, possessed genes encoding rtx proteins, genomic dna was amplified with oligonucleotide primers targeting rtx operon genes of moraxella bovis (m. bovis). complete classical rtx operons composed of rtxcabd genes closely linked ... | 2007 | 17561358 |
| prevention of naturally occurring infectious bovine keratoconjunctivitis with a recombinant moraxella bovis pilin-moraxella bovis cytotoxin-iscom matrix adjuvanted vaccine. | to evaluate the efficacy of a recombinant moraxella bovis pilin-m. bovis cytotoxin subunit vaccine to prevent naturally occurring infectious bovine keratoconjunctivitis (ibk; pinkeye), a randomized, blinded, controlled field trial was conducted during summer 2005 in a northern california herd of beef cattle. one hundred and one steers were vaccinated with iscom matrix (adjuvant control), recombinant m. bovis cytotoxin carboxy terminus+iscom matrix (mbxa), or recombinant m. bovis pilin-cytotoxin ... | 2007 | 17656049 |
| differentiation of moraxella bovoculi sp. nov. from other coccoid moraxellae by the use of polymerase chain reaction and restriction endonuclease analysis of amplified dna. | moraxella ovis was historically the only coccoid moraxella identified in cultures of ocular fluid from cattle with infectious bovine keratoconjunctivitis (ibk) and could be morphologically and biochemically differentiated from moraxella bovis. moraxella bovoculi sp. nov. is a recently characterized moraxella isolated from ulcerated eyes of calves with ibk in northern california in 2002. like moraxella ovis, m. bovoculi sp. nov. is a gram-negative coccus/diplococcus. all 18 original isolates of m ... | 2007 | 17823397 |
| a randomized and blinded field trial to assess the efficacy of an autogenous vaccine to prevent naturally occurring infectious bovine keratoconjunctivis (ibk) in beef calves. | a randomized and blinded 2-arm parallel trial was conducted to assess the efficacy of an autogenous vaccine to prevent naturally occurring infectious bovine keratoconjunctivis (ibk) in beef calves. the trial was managed between may and november 2008 on university owned farms in iowa and wisconsin. the vaccine at iowa contained moraxella bovoculi (m. bovoculi) while the organism used in the wisconsin herds vaccine was branhemella ovis (b. ovis renamed m. ovis). calves born between january and may ... | 2009 | 19531391 |
| cervid herpesvirus 2, the primary agent in an outbreak of infectious keratoconjunctivitis in semidomesticated reindeer. | an outbreak of infectious keratoconjunctivitis (ikc) occurred in semidomesticated reindeer (rangifer tarandus tarandus) in troms county, norway, in february 2009. twenty-eight animals with clinical symptoms and 12 apparently healthy animals were investigated. they ranged in age from calves of the year to 4-year-old animals (mean, 1.9 years; standard deviation, +/-0.9). the seroprevalence of antibodies against cervid herpesvirus 2 (cvhv2) was 86% in animals with ikc and 42% in unaffected animals. ... | 2009 | 19726598 |
| moraxella bovoculi and infectious bovine keratoconjunctivitis: cause or coincidence? | moraxella bovoculi is a recently described gram-negative coccus that was isolated from eyes of calves with infectious bovine keratoconjunctivitis (ibk, or "pinkeye") in 2002. this organism probably has been associated with ibk for many years and, until it was shown to be distinct from m ovis, may have been incorrectly identified as m ovis, branhamella ovis, m ovis-like, or b ovis-like by diagnostic laboratories. m bovoculi can be isolated from normal calves and cattle with ibk or conjunctivitis. ... | 2010 | 20117543 |
| recombinant moraxella bovoculi cytotoxin-iscom matrix adjuvanted vaccine to prevent naturally occurring infectious bovine keratoconjunctivitis. | a randomized, blinded, controlled field trial was conducted during summer 2006 in a northern california, usa, herd of beef cattle to evaluate the efficacy of a recombinant moraxella bovoculi cytotoxin subunit vaccine to prevent naturally occurring infectious bovine keratoconjunctivitis (ibk; pinkeye). a convenience sample comprised of 127 steers were administered a subcutaneous dose of either adjuvant alone (iscom matrices; control group) or recombinant m. bovoculi cytotoxin carboxy terminus adj ... | 2010 | 20217228 |
| ulcerative blepharitis and conjunctivitis in adult dairy cows and association with moraxella bovoculi. | nine lactating dairy cows were evaluated because of eye lesions. examination revealed mild to severe ulceration of the lower and/or upper eyelids, mild to severe swelling surrounding affected eyes, and profuse lacrimation. lesions typically affected 1 eye, and involved the eyelid skin and conjunctiva. oxytetracycline treatment led to cure within 2 wk. | 2010 | 20592830 |
| development and evaluation of a multiplex real-time pcr assay for the detection and differentiation of moraxella bovis, moraxella bovoculi and moraxella ovis in pure culture isolates and lacrimal swabs collected from conventionally raised cattle. | aim:ôçé to develop a multiplex real-time pcr assay for the detection and differentiation of moraxella bovis (m.-ábovis), m.-ábovoculi and m.-áovis. methods and results:ôçé the multiplex real-time pcr assay was validated on three reference strains, 57 pure culture isolates and 45 lacrimal swab samples. all reference strains were identified correctly with no cross-reactions between species. sequencing of 53 of the 57 culture isolates confirmed the results obtained with the multiplex real-time pcr, ... | 2011 | 21827584 |
| minimum inhibitory concentrations of selected antimicrobial agents for moraxella bovoculi associated with infectious bovine keratoconjunctivitis. | infectious bovine keratoconjunctivitis (ibk) has been associated with ocular infections by moraxella bovis, the established etiologic agent of ibk, and more recently, moraxella bovoculi, a recently described species of moraxella. to assist in designing rational treatment regimens for m. bovoculi infections associated with ibk, the in vitro susceptibilities of 57 m. bovoculi field isolates cultured from eyes of cattle with ibk in california from 2002 through 2007 were determined. the minimum inhi ... | 2011 | 21908289 |
| descriptive epidemiology of moraxella bovis, moraxella bovoculi and moraxella ovis in beef calves with naturally occurring infectious bovine keratoconjunctivitis (pinkeye). | problem addressed: infectious bovine keratoconjunctivitis (ibk) is an ocular disease that causes substantial weight loss in beef calves. objective: the objective of this study was to evaluate the association between moraxella bovoculi, moraxella bovis and moraxella ovis and ibk incidence. methods and approach: a cohort design was used. from 239 calves and 478 eyes, 77 randomly chosen eyes were monitored for m. bovoculi, m. bovis, m. ovis and ibk incidence over 4 months. one hypothesis tested was ... | 2011 | 21962410 |
| draft genome sequence of moraxella bovoculi strain 237t (atcc baa-1259t) isolated from a calf with infectious bovine keratoconjunctivitis. | moraxella bovoculi is a recently identified species, recovered from the bovine eye, which is under investigation as an etiological agent of infectious bovine keratoconjunctivitis. a draft genome sequence of the moraxella bovoculi type strain 237(t) has been determined to identify features that may be important during host colonization. | 2014 | 24970830 |
| pain and sickness behavior associated with corneal lesions in dairy calves. | infectious bovine keratoconjunctivitis (ibk) is a common corneal disease of calves that adversely affects animal welfare by causing pain and weight loss. identifying behavioral indicators of pain and sickness in calves with ibk is necessary for designing studies that aim to identify effective means of pain mitigation. consistent with principles of the 3rs for animal use in research, data from a randomized blinded challenge study was used to identify and describe variation of behaviors that could ... | 2015 | 26949517 |
| large genomic differences between moraxella bovoculi isolates acquired from the eyes of cattle with infectious bovine keratoconjunctivitis versus the deep nasopharynx of asymptomatic cattle. | moraxella bovoculi is a recently described bacterium that is associated with infectious bovine keratoconjunctivitis (ibk) or "pinkeye" in cattle. in this study, closed circularized genomes were generated for seven m. bovoculi isolates: three that originated from the eyes of clinical ibk bovine cases and four from the deep nasopharynx of asymptomatic cattle. isolates that originated from the eyes of ibk cases profoundly differed from those that originated from the nasopharynx of asymptomatic catt ... | 2016 | 26872821 |
| phylogenetic analysis and genetic diversity of 3' region of rtxa gene from geographically diverse strains of moraxella bovis, moraxella bovoculi and moraxella ovis. | the cytotoxin a (mbxa) is one of the main virulence factors of moraxella bovis involved in the pathogenesis of infectious bovine keratoconjunctivitis (ibk). moraxella ovis and moraxella bovoculi, suspected to be associated with infectious keratitis in sheep and cattle respectively, also have a gene that encodes the cytotoxin a (mova and mbva, respectively). the aim of this study was to determine the molecular sequence of the 3' region of the cytotoxin gene of moraxella spp. strains isolated from ... | 2015 | 26036790 |
| infectious bovine keratoconjunctivitis (pinkeye). | as is the case for controlling other infectious livestock diseases, the most successful efforts to control infectious bovine keratoconjunctivitis (ibk) will include consideration of the host, the environment, herd management, and ongoing surveillance even after the immediate crisis has passed. research over many years has led to the discovery of a variety of antibiotic treatments and antibiotic regimens that can be effective against ibk. the discoveries of mor bovoculi and reports of ibk associa ... | 2015 | 25576389 |
| moraxella spp. isolated from field outbreaks of infectious bovine keratoconjunctivitis: a retrospective study of case submissions from 2010 to 2013. | infectious bovine keratoconjunctivitis (ibk), also known as pinkeye, is the most costly eye disease of cattle. the principal etiologic agent of ibk is the gram-negative bacterium moraxella bovis. however, there have been reports of ibk outbreaks associated with moraxella bovoculi. a retrospective study of ibk diagnostic cases submitted from july 1, 2010 through october 31, 2013 was conducted. included in the study were 1,042 moraxella isolates from 1,538 swabs of lacrimal secretions collected fr ... | 2014 | 25261461 |
| evaluating approaches to measuring ocular pain in bovine calves with corneal scarification and infectious bovine keratoconjunctivitis-associated corneal ulcerations. | infectious bovine keratoconjunctivitis (ibk) is a common ocular disease in cattle, associated with a 6.8 to 13.6 kg decrease in weaning weight. antibiotic therapy is available but it is unclear if pain mitigation as an adjunct therapy would reduce the weight loss associated with ibk. before assessing the impact of pain mitigation therapies, it is first necessary to validate approaches to qualifying ocular pain. the objective of this study was to evaluate approaches to qualifying ocular pain in b ... | 2014 | 24504043 |
| diversity of moraxella spp. strains recovered from infectious bovine keratoconjunctivitis cases in uruguay. | infectious bovine keratoconjunctivitis (ibk) is the most common ocular disease that affects cattle throughout the world and it has a very significant economic impact. ibk is caused by members of the genus moraxella and therapeutic and preventive measures have shown limited success. vaccines, most of them chemically inactivated bacterins, generally induce a limited protection. | 2013 | 24240039 |
| randomized blinded challenge study to assess association between moraxella bovoculi and infectious bovine keratoconjunctivitis in dairy calves. | the objective of this study was to evaluate if moraxella bovoculi was associated with infectious bovine keratoconjunctivitis (ibk) using a corneal scarification model in calves. a 3-arm single-eye block-randomized and blinded challenge study was designed as follows: corneal scarification only, corneal scarification and inoculation with m. bovoculi (atcc strain: baa-1259; origin: ca) and corneal scarification and inoculation with moraxella bovis (strain epp63-300; origin: nadc). the study was con ... | 2013 | 23452750 |
| randomized controlled field trial to assess efficacy of a moraxella bovis pilin-cytotoxin-moraxella bovoculi cytotoxin subunit vaccine to prevent naturally occurring infectious bovine keratoconjunctivitis. | to evaluate efficacy of a recombinant moraxella bovis pilin-cytotoxin-moraxella bovoculi cytotoxin subunit vaccine to prevent naturally occurring infectious bovine keratoconjunctivitis (ibk). | 2012 | 23013196 |
| evaluation of conjunctival bacterial flora in a herd of goats in the midwestern united states. | to identify conjunctival bacterial flora in healthy adult and newborn kid goats in the midwestern united states and to compare vaginal and ocular surface flora in dam-kid pairs. | 2017 | 26778666 |
| isolation of moraxella bovoculi from racehorses with keratoconjunctivitis. | moraxella bovoculi was isolated and identified in ocular fluid samples collected from 9 racehorses with infectious keratoconjunctivitis in china in 2013. all 9 m. bovoculi isolates were hemolytic, gram-negative diplococci that were phenylalanine deaminase positive. the sequence of the 16s ribosomal dna (rdna) gene of the isolates matched the 16s rdna sequence of m. bovoculi. amplification of the 16s-23s intergenic spacer region followed by afai digestion produced a 600-base pair product, a resul ... | 2014 | 24903634 |
| cervid herpesvirus 2 and not moraxella bovoculi caused keratoconjunctivitis in experimentally inoculated semi-domesticated eurasian tundra reindeer. | infectious keratoconjunctivitis (ikc) is a transmissible disease in semi-domesticated eurasian reindeer (rangifer tarandus tarandus). it is regarded as multifactorial and a single causative pathogen has not yet been identified. from clinical outbreaks we have previously identified cervid herpesvirus 2 (cvhv2) and moraxella bovoculi as candidates for experimental investigations. eighteen reindeer were inoculated in the right eye with cvhv2 (n = 5), m. bovoculi (n = 5), cvhv2 and m. bovoculi (n = ... | 2017 | 28438213 |