| determination of interconversion barriers by dynamic gas chromatography: epimerization of chalcogran. | the four stereoisomers of chalcogran 1 ((2rs,srs)-2-ethyl-1,6-di-oxaspiro[4.4]nonane), the principal component of the aggregation pheromone of the bark beetle pityogenes chalcographus, are prone to interconversion at the spiro center (c5). during diastereo- and enantioselective dynamic gas chromatography (dgc), epimerization of 1 gives rise to two independent interconversion peak profiles, each featuring a plateau between the peaks of the interconverting epimers. to determine the rate constants ... | 2001 | 11330904 |
| what is next in bark beetle phylogeography? | bark beetle species within the scolytid genera dendroctonus, ips, pityogenes and tomicus are known to cause extensive ecological and economical damage in spruce and pine forests during epidemic outbreaks all around the world. dendroctonus ponderosae poses the most recent example having destroyed almost 100,000 km² of conifer forests in north america. the success and effectiveness of scolytid species lies mostly in strategies developed over the course of time. among these, a complex system of sem ... | 2012 | 26466538 |
| mitochondrial phylogenies in the light of pseudogenes and wolbachia: re-assessment of a bark beetle dataset. | phylogenetic studies based on mtdna become increasingly questioned because of potential pitfalls due to mitochondrial pseudogenes and mitochondrial selective sweeps. while the inclusion of nuclear markers should preferentially be considered for future studies, there is no need to abandon mtdna as long as tests for the known mtdna artefacts are performed. in this study we presentadditionaldata and test previous phylogeographical studies of pityogenes chalcographus. we did not detect nuclear copie ... | 2010 | 21594185 |
| evidence for low-titre infections in insect symbiosis: wolbachia in the bark beetle pityogenes chalcographus (coleoptera, scolytinae). | wolbachia are obligatory endosymbiotic alpha-proteobacteria found in many insect species. they are maternally transmitted and often exhibit reproductive phenotypes like cytoplasmic incompatibility. pityogenes chalcographus is a bark beetle causing severe damage in spruce stands. its european populations are divided into several mitochondrial clades separated by partial crossing barriers. in this study, we tested a large sample set covering the natural range of the beetle in europe for the presen ... | 2009 | 19383035 |
| lessons from a beetle and an ant: coping with taxon-dependent differences in microsatellite development success. | microsatellites are powerful markers often isolated de novo for species yet to be investigated. enriched genomic libraries are usually used for isolation purposes. we critically evaluate the outcome of an enrichment-based protocol applied to two insect species (the ant lasius austriacus and the beetle pityogenes chalcographus) which yielded contrasting numbers of suitable loci. our findings of differences in microsatellite isolation are consistent with the available data on differences in genomi ... | 2007 | 17726568 |
| fast establishment of single-strand conformation polymorphism by targeted primer development in pityogenes chalcographus (coleoptera, scolytinae). | sscp is a widespread method for mutation detection in biomedical research. yet, its potential as a tool for population genetics is still not fully utilized. based on mitochondrial dna sequences of 96 specimens of the wood-boring beetle pityogenes chalcographus we constructed a phylogenetic tree of european populations. this tree consisted of six broadly sympatric diverged lineages containing in total 34 haplotypes. genetic regions of high mutational activity were determined and used for targeted ... | 2007 | 17311243 |