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[examination of market carps for residues of antimicrobial agents].the residues of inhibitory substances in the muscle, hepatopancreas, bile, and pyloric caeca of 30 carps from ponds south of brno were examined by the microbiological diffusion method. the following testing micro-organisms were used: b. cereus var. mycoides (atcc 11778), b. subtilis (atcc 6633), sarcina lutea (atcc 9341), and micrococcus flavus (atcc 10240). residues of inhibitory substances were not found in any of the muscle samples tested. the rest of the tested tissues showed, in some cases, ...1978100930
viable but nonculturable bacteria in drinking water.klebsiella pneumoniae, enterobacter aerogenes, agrobacterium tumefaciens, streptococcus faecalis, micrococcus flavus, bacillus subtilis, and pseudomonas strains l2 and 719 were tested for the ability to grow and maintain viability in drinking water. microcosms were employed in the study to monitor growth and survival by plate counts, acridine orange direct counts (aodc), and direct viable counts (dvc). plate counts dropped below the detection limit within 7 days for all strains except those of b ...19912039237
effect of organic amendments on bacterial multiplication in lake water.in cayuga lake water amended with 30 micrograms of glucose or amino acids per ml, an added strain of pseudomonas fluorescens and indigenous bacteria grew extensively, pseudomonas sp. b4 and two rhizobia multiplied at a moderate extent, and introduced escherichia coli and klebsiella pneumoniae multiplied but to only a slight degree. the amendments did not enhance growth of micrococcus flavus and arthrobacter citreus, and an asporogenous strain of bacillus subtilis decreased in numbers. the pseudo ...19902372214
factors affecting the survival and growth of bacteria introduced into lake water.the populations of pseudomonas sp. b4, escherichia coli, klebsiella pneumoniae, micrococcus flavus, and rhizobium leguminosarum biovar phaseoli declined rapidly in lake water. the initially rapid decline of the two pseudomonads and r. phaseoli was followed by a period of slow loss of viability, but viable cells of the other species were not found after 10 days. the rapid initial phase of decline was not a result of bdellovibrio spp., bacteriophages, or toxins in the water since bdellovibrio spp. ...19883060035
role of sublethal injury in decline of bacterial populations in lake water.following their addition to lake water, the populations of escherichia coli and of antibiotic-resistant strains of pseudomonas fluorescens, agrobacterium tumefaciens, micrococcus flavus, rhizobium meliloti, and klebsiella pneumoniae declined rapidly, as determined by counting on media containing antibacterial compounds. the estimates of population sizes were occasionally higher if procedures were used that permitted possible resuscitation of injured cells. no resuscitation procedure yielded cons ...19883145714
effect of monovalent and divalent ions upon the activity of nisin against micrococcus flavus. 19695787733
[effect of solid substrates of microbial colony development].bacteria have been found to grow differently at contact sites to both organic and anorganic solids as on pure agar media. of 16 ad hoc selected bacterial strains, the following species (arranged in decreasing order) responded with stronger colony formation: bacillus subtilis, b. cereus, streptococcus sanguis, s. saprophyticus, s. faecalis, escherichia coli, micrococcus flavus, actinomyces viscosus, veillonella sp., enterobacter liquefaciens, yersinia enterocolitica. lactobacillus casei behaved n ...19846326398
molecular properties of succinate dehydrogenase isolated from micrococcus luteus (lysodeikticus).succinate dehydrogenase (ec 1.3.99.1) of micrococcus luteus was selectively precipitated from triton x-100-solubilized membranes by using specific antiserum. the precipitated enzyme contained equimolar amounts of four polypeptides with apparent molecular weights of 72,000, 30,000, 17,000, and 15,000. the 72,000 polypeptide possessed a covalently bound flavin prosthetic group and appeared to be strongly antigenic as judged by immunoprinting experiments. low-temperature absorption spectroscopy rev ...19836402500
bacitracin production by a new strain of bacillus subtilis. extraction, purification, and characterization.a new strain of bacillus subtilis c 126 was isolated from sugar cane fermentation and produced an antibiotic that inhibited the growth of micrococcus flavus. the production of the antibiotic in culture medium followed to extraction with n-butanol, thin layer chromatography, and microbiological tests indicated that a polypeptide antibiotic was produced. the fraction obtained by sephadex g-25 column and analyzed by hplc indicated that bacitracin complex was produced.19938215347
construction of a single-copy integration vector and its use to study gene expression in bacillus licheniformis.a versatile system consisting of an integrational vector and a bacitracin (bt)-producing beta-galactosidase (beta-gal)-negative (lac-) bacillus licheniformis tlh strain was constructed to quantify promoter activity and to study gene regulation in a single-copy set-up. the vector ptlh utilizes the promoterless escherichia coli lacz gene derived from pqf52 and contains the pbr322 origin of replication and a kanamycin-resistance gene for selection in both b. licheniformis and e. coli. the vector al ...19989782506
aspects of the antimicrobial efficacy of grapefruit seed extract and its relation to preservative substances contained.the antimicrobial efficacy as well as the content of preservative agents of six commercially available grapefruit seed extracts were examined. five of the six extracts showed a high growth inhibiting activity against the test germs bacillus subtilis sbug 14, micrococcus flavus sbug 16, staphylococcus aureus sbug 11, serratia marcescens sbug 9, escherichia coli sbug 17, proteus mirabilis sbug 47, and candida maltosa sbug 700. in all of the antimicrobial active grapefruit seed extracts, the preser ...199910399191
cyanobacteria--a potential source of new biologically active substances.hydrophilic and lipophilic extracts of twelve cyanobacterial strains, isolated from fresh and brackish water, and two waterblooms, collected during the summer from the baltic sea, were investigated for their antibiotic activities against seven microorganisms. no inhibitory effects were found against the three gram-negative bacteria escherichia coli, proteus mirabilis and serratia marcescens and the yeast candida maltosa. of all cyanobacterial samples, extracts from seven species inhibited the gr ...199910412206
synthesis and antimicrobial activity of new adamantane derivatives i.a series of fourteen derivatives of adamantane was synthesised. the new compound 4-(adamant-1-ylmethoxycarbonyl)phthalanhydride obtained from 1-adamantane-methanol and trimellitic anhydride chloride appeared very useful for preparation of a number of n-substituted phthalimides. antimicrobial activity of the newly obtained derivatives such as, for example, 4-(adamant-1-ylmethoxycarbonyl)-n-(5-carboxypentamethylene)p hthalimide or 4-(adamant-1-ylmethoxycarbonyl)-n-(l-alanyl)phthalimide was tested ...200010961681
identification and partial characterization of lacticin bh5, a bacteriocin produced by lactococcus lactis bh5 isolated from kimchi.strain bh5 was isolated from naturally fermented kimchi and identified as a bacteriocin producer that has bactericidal activity against micrococcus flavus atcc 10240. strain bh5 was identified tentatively as lactococcus lactis by api test. lactococcus lactis bh5 showed a broad spectrum of activity against most of the nonpathogenic and pathogenic microorganisms tested by the modified deferred method. the activity of lacticin bh5, named tentatively as the bacteriocin produced by l. lactis bh5, was ...200011131895
synthesis and antimicrobial activity of new adamantane derivatives ii.a series of new derivatives of adamantane was synthesised. the new compound 4-(adamant-1-ylethylenoxycarbonyl)phthalanhydride obtained from 1-adamantaneethanol and trimellitic anhydride chloride, as well as 4-(adamant-1-ylmethylenoxycarbonyl)phthalanhydride, appeared very useful for preparation of a number of n-substituted phthalimides. antimicrobial activity of newly obtained derivatives such as, for example, 4-(adamant-1-ylethylenoxycarbonyl)-n-(l-phenylalanyl)phthalimide or 4-(adamant-1-ylmet ...200011152243
planetary quarantine in the solar system. survival rates of some terrestrial organisms under simulated space conditions by proton irradiation.we have been studying the survival rates of some species of terrestrial unicellular and multicellular organism (viruses, bacteria, yeasts, fungi, algae, etc.) under simulated interstellar conditions, in connection with planetary quarantine. the interstellar environment in the solar system has been simulated by low temperature, high vacuum (77 k, 4 x 10(-8) torr), and proton irradiation from a van de graaff generator. after exposure to a barrage of protons corresponding to about 250 years of irra ...199311541644
antimicrobial activity of polyscias filicifolia cell biomass extracts.antibacterial activity of extracts of polyscias filicifolia biomass from bioreactor and callus was determined using the agar disc-diffusion method. the microorganisms staphylococcus aureus (three strains) showed the highest sensitivity to extracts of p. filicifolia biomass from a bioreactor. the values were comparable with nitrofurantoine used as a standard. micrococcus flavus, sreptococcus pyogenes and s. agalatiae were less sensitive. the effect of p. filicifolia callus extract on the above ba ...200212116883
the antibacterial activity of topical retinoids: the case of retinaldehyde.retinoids such as retinoic acid (ra), retinol (rol) and retinaldehyde (ral) are currently used in many formulations and indications ranging form acne to skin aging. most if not all their pharmacological activities occur through binding to nuclear receptors with subsequent modulation of the activities of several genes. little attention has been given to the many other potential actions on the surface of the skin.200212218231
antimicrobial and antioxidant activities of three mentha species essential oils.the present study describes the antimicrobial activity and free radical scavenging capacity (rsc) of essential oils from mentha aquatica l., mentha longifolia l., and mentha piperita l. the chemical profile of each essential oil was determined by gc-ms and tlc. all essential oils exhibited very strong antibacterial activity, in particularly against esherichia coli strains. the most powerful was m. piperita essential oil, especially towards multiresistant strain of shigella sonei and micrococcus ...200312802721
the international standard for bacitracin.in 1952, the department of biological standards, national institute for medical research, london, was authorized by the who expert committee on biological standardization to proceed with the establishment of an international standard for bacitracin. thirty grams of bacitracin were obtained and it was decided to compare the proposed international standard with the existing working standard for bacitracin of the united states food and drug administration.six laboratories in five countries particip ...195313141138
essential oil analysis and antimicrobial activity of eight stachys species from greece.the volatile composition of eight stachys species has been studied. the investigated taxa were st. alopecuros (l.) bentham., st. scardica (griseb.) hayek, st. cretica l. ssp. cretica, st. germanica l. ssp. heldreichii (boiss.) hayek, st. recta l., st. spinulosa l., st. euboica rech. and st. menthifolia vis., growing wild in greece. the essential oils were obtained by hydrodistillation in a modified clevenger-type apparatus, and their analyses were performed by gc and gc-ms. identification of the ...200313679097
taxonomically significant color reactions of brevibacterium linens.grecz, nicholas (university of chicago, chicago, ill.), and gail m. dack. taxonomically significant color reactions of brevibacterium linens. j. bacteriol. 82:241-246. 1961.-brevibacterium linens was observed to give characteristic color reactions with certain bases and acids. an intensive carmine-red color appeared immediately after addition of a drop of 5 n sodium hydroxide, 5 n potassium hydroxide, and saturated barium hydroxide. a light carmine-red was given by lithium hydroxide, and a light ...196113708147
site-directed mutagenesis of the hinge region of nisinz and properties of nisinz mutants.to study the role of the hinge region in nisin and to obtain mutants that exhibit altered or new biological activities and functional properties, we changed certain amino acids in the hinge region by performing site-directed mutagenesis with the nisinz structural gene ( nisz). the results showed that the nisinz mutants had decreased antimicrobial activities against micrococcus flavus ncib8166 and streptococcus thermophilus. interestingly, compared with wild nisinz, mutant n20k nisinz and m21k ni ...200415048591
bacterial inhibitors in lake water.the populations of six bacterial genera fell rapidly after their addition to sterile lake water but not after their addition to buffer. the decline in numbers of two species that were studied further, klebsiella pneumoniae and micrococcus flavus, occurred even when the buffer was added to sterile lake water. the inhibition of k. pneumoniae by substances in lake water varied with the season of the year, and the rate and extent of decline of both species were different in sterile samples of differ ...198616347097
bioactive components of the traditionally used mushroom podaxis pistillaris.in the course of an ethnobotanical study on fungi used in yemeni ethnomedicine the fungus podaxis pistillaris (podaxales, podaxaceae, basidiomycetes) was found to exhibit antibacterial activity against staphylococcus aureus, micrococcus flavus, bacillus subtilis, proteus mirabilis, serratia marcescens and escherichia coli. in the culture medium of p. pistillaris three epidithiodiketopiperazines were identified by activity-guided isolation. based on spectral data (nmr, esi-ms and dci-ms) their id ...200616550228
potential of lactic acid bacteria isolated from specific natural niches in food production and preservation.autochthonous strains of lactic acid bacteria (lab) have been isolated from traditionally homemade cheeses collected from specific ecological localities across serbia and montenegro. genetic and biochemical analysis of this lab revealed that they produce bacteriocins, proteinases and exopolysaccharides. lab produces a variety of antimicrobial substances with potential importance for food fermentation and preservation. apart from the metabolic end products, some strains also secrete antimicrobial ...200616764959
fluorescent reagents for in vitro studies of lipid-linked steps of bacterial peptidoglycan biosynthesis: derivatives of udpmurnac-pentapeptide containing d-cysteine at position 4 or 5.udpmurnac-l-ala-gamma-d-glu-x-d-ala-dala (x = l-lys or m-dap) is the cytoplasmic precursor for the lipid-linked cycle of bacterial peptidoglycan biosynthesis, consisting of at least four enzymatic reactions, which are targets for antibacterial agents. fluorescent derivatives of the udpmurnac-pentapeptide labelled at the 3rd, 4th, and 5th position of the peptide chain were prepared chemoenzymatically, in order to study the reactions catalysed by enzymes in this cycle. derivatives labelled on the ...200617216029
separation and estimation of purine and pyrimidine bases from a heated suspension of micrococcus flavus. 196013704731
molecular and biochemical characterizations of human oral lactobacilli as putative probiotic candidates.the objective of this study was to characterize the lactobacilli from the human oral cavity as a potential source of probiotic strains.200717311634
membrane activity of the peptide antibiotic clavanin and the importance of its glycine residues.the peptide antibiotic clavanin a (vfqflgkiihhvgnfvhgfshvf-nh(2)) is rich in histidine and glycine residues. in this study the antimicrobial activity and membrane activity of wild-type clavanin a and seven gly --> ala mutants thereof were investigated. clavanin a effectively killed the test microorganism micrococcus flavus and permeabilized its cytoplasmic membrane in the micromolar concentration range, suggesting that the membrane is the target for this molecule. consistent with this suggestion ...200111371202
marine algae: screening for a potent antibacterial agent.this study was done to investigate the antimicrobial potentiality of the marine algae collected from different coastal regions of gujarat and screened for the same. twenty-six marine algae belonging to rhodophyceae, chlorophyceae and phaeophyceae were screened for their potential antibacterial activity against five clinically important bacterial strains, namely bacillus cereus, micrococcus flavus, citrobacter freundii, klebsiella pneumoniae and pseudomonas testosterone. acetone and methanol were ...200717594989
purification of bacteriocin ls1 produced by human oral isolate lactobacillus salivarius bgho1.lactobacillus salivarius bgho1, a human oral isolate with antagonistic activity against growth of streptococcus mutans, streptococcus pneumoniae, staphylococcus aureus, enterococcus faecalis, micrococcus flavus, and salmonella enteritidis, probably produces more than one proteinaceous antimicrobial substance. the objective of this study was the purification of a bacteriocin, named ls1, produced by l. salivarius bgho1.200818402613
production, purification and chemical characterization of the catecholate siderophore from potent probiotic strains of bacillus spp.the aim of the present study was to characterize the probiotic qualities of bacillus isolates and study their siderophore prior to possible siderophoregenic probiotic application for iron nutrition in animals and humans. bacillus strains were selectively isolated from dairy waste and mango pulp waste. best two siderophore positive isolates, jht3 and det6 showed high homology with bacillus megaterium (98%) and b. subtilis (99%), respectively, using partial 16s-rrna sequencing and biochemical char ...200918585911
antibacterial, antioxidant and fibroblast growth stimulation of aqueous extracts of ficus asperifolia miq. and gossypium arboreum l., wound-healing plants of ghana.use of in vitro tests to search for relevant activities in bark of ficus asperifolia miq. and leaves of gossypium arboreum l., used in ghana for wound healing.200818638536
activity of cytotoxin p4 from the venom of the cobra snake naja nigricollis on gram-positive bacteria and eukaryotic cell lines.the cytotoxin p4 from the venom of the cobra snake naja nigricollis was examined for activity against aerobic gram-positive and gram-negative bacteria, yeasts, fungi and eucaryotic cell lines by determination of minimal inhibitory concentrations (mic) and ic50 values, respectively. cytotoxin p4 exhibits antimicrobial activity against bacillus subtilis, micrococcus flavus and sarcina lutea. targets in gram-negative bacteria and fungi apparently were not reached. toxicity against eucaryotic cells ...19979205785
antibacterial activity of some plant extracts used in folk medicine.in the present work, selected plants were screened for their potential antibacterial activity. for evaluating antibacterial activity, both aqueous and organic solvent methanol was used. the plants screened were ocimum sanctum, jatropha gossypifolia, boerhavia diffusa, azadirachta indica, solidago virgaurea, and commelina benghalensis. the antibacterial activity was assessed against six bacterial strains--pseudomonas testosteroni, staphylococcus epidermidis, klebsiella pneumoniae, bacillus subtil ...200718928141
role of resistance to starvation in bacterial survival in sewage and lake water.a study was conducted to determine the significance of starvation resistance to the ability of a species to survive in sewage and lake water. tests were conducted for periods of up to 14 days. rhizobium meliloti and one fluorescent and one nonfluorescent strain of pseudomonas were resistant to starvation because their population sizes did not fall appreciably in buffer and sterile lake water, and the first two maintained high numbers after being added to sterile sewage. cell densities of these b ...19846435525
composition and antimicrobial activity of marrubium incanum desr. (lamiaceae) essential oil.the essential oil from the aerial parts of marrubium incanum desr. (lamiaceae), obtained by hydrodistillation, was analyzed by gc and gc-ms. forty-six compounds were identified, representing 96.3% of the total oil. the main components of the oil were (e)-caryophyllene (27.0%), germacrene d (26.2%) and bicyclogermacrene (11.5%). the microbial growth inhibitory properties of the isolated essential oil were determined using the agar diffusion and broth microdilution method against seven bacterial s ...200919413128
dimension control in micrococcus flavus. 19725086109
production of a class ii two-component lantibiotic of streptococcus pneumoniae using the class i nisin synthetic machinery and leader sequence.recent studies showed that the nisin modification machinery can successfully dehydrate serines and threonines and introduce lanthionine rings in small peptides that are fused to the nisin leader sequence. this opens up exciting possibilities to produce and engineer larger antimicrobial peptides in vivo. here we demonstrate the exploitation of the class i nisin production machinery to generate, modify, and secrete biologically active, previously not-yet-isolated and -characterized class ii two-co ...201020100873
antimicrobial activity of the extracts of craniotome furcata (lamiaceae).craniotome furcata (link.) o. kuntze (lamiaceae) is used in the traditional medicine for the treatment of cuts and wounds indicating antimicrobial activity.201020176098
screening of the constituents, antimicrobial and antioxidant activity of endemic origanum hypericifolium o. schwartz & p.h. davis.the chemical compositions, total phenol content, antioxidant and antimicrobial activities with oxidant status of the essential oil from an endemic turkish species, origanum hypericifolium, were investigated. steam distillation (sd) was used to isolate the essential oils, and the chemical analyses were performed by gas chromatography-mass spectrometry (gc-ms). the antimicrobial activity was tested by agar disc diffusion method against morganella morganii (clinic isolate), micrococcus flavus (clin ...201020835958
antibacterial effects of the essential oils of commonly consumed medicinal herbs using an in vitro model.the chemical composition and antibacterial activity of essential oils from 10 commonly consumed herbs: citrus aurantium, c. limon, lavandula angustifolia, matricaria chamomilla, mentha piperita, m. spicata, ocimum basilicum, origanum vulgare, thymus vulgaris and salvia officinalis have been determined. the antibacterial activity of these oils and their main components; i.e. camphor, carvacrol, 1,8-cineole, linalool, linalyl acetate, limonene, menthol, a-pinene, b-pinene, and thymol were assayed ...201021030907
evaluation of chicken embryo, brine shrimp, and bacterial bioassays for saxitoxin.the chicken embryo, brine shrimp (artemia salina) and selected bacteria (staphylococcus epidermidis, micrococcus flavus, and bacillus subtilis) were evaluated as alternative test systems for the determination of paralytic shellfish poisoning toxicity (saxitoxin). dose levels ranging from 0.045 to 0.3 microgram were administered to the developing embryo through the air cell at either 0 or 96 h following incubation. embryos dosed 96 h after incubation were the most sensitive, with 100% mortality a ...19863090272
wound healing properties and kill kinetics of clerodendron splendens g. don, a ghanaian wound healing plant.as part of our general objective of investigating indigenous plants used in wound healing in ghana, we hereby report our findings from some in vitro and in vivo studies related to wound healing activities of clerodendron splendens g. don (verbanaceae). methanolic extract of the aerial parts of the plant was tested for antimicrobial activity against gram positive bacteria (bacillus subtilis, staphylococcus aureus, streptococcus faecalis, micrococcus flavus, as well as resistant strains of staph. ...201021808542
Composition and antimicrobial activity of Seseli globiferum essential oil.The essential oil from aerial parts of Seseli globiferum Vis. obtained by hydrodistillation with Clevenger-type apparatus was analyzed by GC-MS. Twenty-eight compounds were identified, representing 99.4% of the total oil. The main components of the oil were sabinene (38.0%), alpha-pinene (21.2%) and beta-phellandrene (13.5%). The microbial growth inhibitory properties of the isolated essential oil were determined using the broth microdilution method against seven bacterial species: Salmonella ty ...201121922927
improved microbiological procedure for determining bacitracin in premixes and mixed feeds.an improved microbiological procedure is presented for determining bacitracin in premixes and mixed feeds. premixes are extracted with acidic 50% aqueous methanol, and mixed feeds are extracted with 50% dimethylformamide after washing with acetone to remove fats and pigments. extracts are diluted with ph 6.0 phosphate buffer and assayed by using a 2-point assay system with micrococcus flavus as the test organism. the simpler extraction coupled with the 2-point assay system measured bacitracin le ...1976965335
[residues of chloramphenicol in the calf organism following parenteral administration of the czechoslovak preparation chronicin inj].using two biological methods the chloramphenicol content in various tissues of 11 calves aged 3--18 days was determined after a single-dose therapeutical administration of the czechoslovak preparation of chronicin inj. in the quantity of 12 mg kg-1 body mass. of four test strains, solely micrococcus flavus (atcc 10240) strain proved suitable. chloramphenicol was quantitatively determined by reading from transformed standard curves. an evalua tion of the two approximately equally sensitive metho ...1976820050
acorus calamus linn.: phytoconstituents and bactericidal property.acorus calamus linn. of the family araceae (acoraceae), commonly known as sweet flag and vacha. the rhizome of this plant has medicinal properties against bugs, moths, lice and emetic stomach in dyspepsia. chemical composition of the hydro-distilled essential oil obtained from the rhizomes of a. calamus was analyzed by gas chromatography equipped with flame ionization detector and gas chromatography coupled with mass spectrometry. the essential oil of a. calamus and its major compound β-asarone ...201627562598
antimicrobial activity of the pygidial gland secretion of the troglophilic ground beetle laemostenus (pristonychus) punctatus (dejean, 1828) (insecta: coleoptera: carabidae).the antimicrobial activity of the pygidial gland secretion released by adult individuals of the troglophilic ground beetle laemostenus (pristonychus) punctatus (dejean, 1828), applying microdilution method with the aim to detect minimal inhibitory concentration, minimal bactericidal concentration and minimal fungicidal concentration, has been investigated. in addition, morphology of the pygidial glands is observed. we have tested 16 laboratory and clinical strains of human pathogens - eight bact ...201627018928
volatile composition and antimicrobial activity of the essential oil of artemisia absinthium growing in western ghats region of north west karnataka, india.artemisia absinthium l. (asteraceae) is an aromatic, herbaceous, perennial plant commonly known as wormwood. artemisia absinthium is traditionally used as an anthelmintic, antiseptic, antispasmodic and for bacillary dysentery, cancers and neurodegenerative diseases.201323570523
biological activities of selected basidiomycetes from yemen.in a previous paper we demonstrated the results of biological screening of yemeni basidiomycetes. the present study was aimed to investigate the antimicrobial and the antioxidant activity of further basidiomycetes collected in yemen. dichloromethane, methanol and aqueous extracts of the fruiting bodies of 25 species were screened in vitro for their antibacterial activities against three gram-positive bacteria (staphyloccocus aureus, bacillus subtilis, micrococcus flavus) and two gram-negative ba ...201323556343
composition, antioxidant and antimicrobial activities of methanol extracts of some acinos miller species.gc and gc/ms analyses of the methanol extracts obtained from the aerial parts of six acinos miller species from serbia and montenegro were performed. seventy-four constituents, accounting for 84.9-99.0% of the total composition of the extracts, were identified. the common feature of the a. suaveolens, a. majoranifolius and a. alpinus methanol extracts was the high content of monoterpenes, while the common feature of the a. graveolens, a. arvensis and a. hungaricus methanol extracts was the preva ...201425026735
broad-spectrum of antimicrobial properties of commercial wines from different vitis vinifera l. varieties.fifteen commercial wines produced from international and autochthonic varieties of vitis vinifera l. cultivation of different balkan winegrowing subregions were studied for their antimicrobial activity against six gram-positive (clostridium perfringens, bacillus subtilis, staphylococcus aureus, listeria inocua, sarcina lutea and micrococcus flavus) and six gram-negative (escherichia coli, pseudomonas aeruginosa, salmonella enteritidis, shigella sonnei, klebsiella pneumonia and proteus vulgaris) ...201727905092
essential oil of eucalyptus gunnii hook. as a novel source of antioxidant, antimutagenic and antibacterial agents.the present study describes radical scavenging capacity (rsc), antimutagenic and antibacterial properties of the essential oil (eo) of the leaves of eucalyptus gunnii hook. (southern montenegro). chemical composition was evaluated by gas chromatography-mass spectrometry (gc-ms). in oil, 1,8-cineole (67.8%) and α-pinene (14.12%) were the major compounds comprising almost 82% of total eo. eo exhibited moderate dpph (2,2-diphenyl-1-picrylhydrazyl) scavenging activity, with ic50 value of 7.19 µl/ml. ...201425412046
[persistance of penicillin residues in the calf organism after parenteral therapeutical administration of the preparation penicillin g, procaine inj ad usum vet].by means of two biological diffusion methods a penicillin content in various calf tissues was determined. for the injections prokain penicillin g spofa, inj. adusum vet., was used in a single dose of 25 000 i. u. kg-1 body mass. for the tests three collection strains -- b. subtilis (atcc 6633), sarcina lutea (atcc 9341), and micrococcus flavus (atcc 10240) were used. a quantitative determination of penicillin in the samples was accomplished by reading from the standard curves. sensitivities of t ...1976820048
micrococcus flavus sp. nov., isolated from activated sludge in a bioreactor.bacterial strain lw4(t) was isolated from activated sludge of a wastewater-treatment bioreactor. cells of strain lw4(t) were gram-positive cocci, with a diameter of 0.7-1.0 microm. colonies produced on lb agar plates were yellow, smooth, circular and 0.5-1.5 mm in diameter. strain lw4(t) was aerobic and grew over the temperature range 26-34 degrees c and ph range 5-9, with optimal growth at 30.5-31.5 degrees c and ph 6.0-6.2. the cell-wall peptidoglycan of strain lw4(t) contained amino acid resi ...200717220443
overexpression, purification, and characterization of atp-nad kinase of sphingomonas sp. a1.the nad kinase gene (nadk) of sphingomonas sp. a1 was cloned and then overexpressed in escherichia coli, and the gene product (nadk) was purified from the e. coli cells through five steps with a 25% yield of activity. nadk was a homodimer of 32 kda subunits, utilized atp or other nucleoside triphosphates, but not inorganic polyphosphates, as phosphoryl donors for the phosphorylation of nad, most efficiently at ph 8.0 and 50-55 degrees c, and was designated as atp-nad kinase (nadk). nadk showed n ...200415177293
antioxidant and antimicrobial potentials of serbian red wines produced from international vitis vinifera grape varieties.antioxidant and antimicrobial potentials of serbian red wines produced from different international vitis vinifera grape varieties and their correlation with contents of phenolic compounds were studied by spectrophotometric and chromatographic methods. the antioxidant activity of red wines was estimated through their ability to scavenge 2,2'-diphenyl-1-picrylhydrazyl free radical (dpph(•) ). the red wines, gallic acid, (+)-catechin and quercetin were screened in vitro for antimicrobial activity ...201222318878
frozen suspensions from micrococcus flavus for use in the microbiological assay of bacitracin. 195224542147
bovicin hj50, a novel lantibiotic produced by streptococcus bovis hj50.a bacteriocin-producing strain was isolated from raw milk and named streptococcus bovis hj50. like most bacteriocins produced by lactic acid bacteria, bovicin hj50 showed a narrow range of inhibiting activity. it was sensitive to trypsin, subtilisin and proteinase k. bovicin hj50 was extracted by n-propanol and purified by sp sepharose fast flow, followed by phenyl superose and sephadex g-50. treatment of micrococcus flavus ncib8166 with bovicin hj50 revealed potassium efflux from inside the cel ...200414702402
primary structure of inorganic polyphosphate/atp-nad kinase from micrococcus flavus, and occurrence of substrate inorganic polyphosphate for the enzyme.the gene encoding an inorganic polyphosphate/atp-nad kinase was cloned from micrococcus flavus, and its primary structure was analyzed. alignment of the primary structure with those of other characterized nad kinases revealed candidate amino acid residues, mainly charged ones, that would be related to inorganic polyphosphate use. the alignment also showed that the primary structure found carried a protruding c-terminal polypeptide. although the c-terminal polypeptide was demonstrated to be dispe ...200312951510
anti-inflammatory, gastroprotective, free-radical-scavenging, and antimicrobial activities of hawthorn berries ethanol extract.hawthorn [crataegus monogyna jacq. and crataegus oxyacantha l.; sin. crataegus laevigata (poiret) dc., rosaceae] leaves, flowers, and berries are used in traditional medicine in the treatment of chronic heart failure, high blood pressure, arrhythmia, and various digestive ailments, as well as geriatric and antiarteriosclerosis remedies. according to european pharmacopoeia 6.0, hawthorn berries consist of the dried false fruits of these two species or their mixture. the present study was carried ...200818698794
formation and efficacy of vancomycin group glycopeptide antibiotic stereoisomers studied by capillary electrophoresis and bioaffinity mass spectrometry.the conformational stability of vancomycin group antibiotics (i.e., vancomycin and avoparcin) in aqueous solution has been studied. these complex glycopeptide antibiotics contain many chiral centers allowing the potential formation of stereoisomers. using capillary electrophoresis these stereoisomers could be separated and detected by uv and/or mass spectrometry. fresh aqueous samples of both vancomycin and avoparcin already contained a plethora of stereoisomers. thermal degradation of the antib ...200111237332
a chelating agent possessing cytotoxicity and antimicrobial activity: 7-morpholinomethyl-8-hydroxyquinoline.7-morpholinomethyl-8-hydroxyquinoline (mo-8hq), which like 8-hydroxyquinoline (8hq) readily forms a chelate, was synthesized and found to possess cytotoxicity and antimicrobial activity. both 8hq and mo-8hq were cytotoxic to human carcinoma cell lines at micromolar concentrations. mo-8hq also inhibited dna synthesis of tumor cells at micromolar concentrations, suggesting that mo-8hq might chelate metals necessary for the enzymatic catalysis of dna biosynthesis. mo-8hq was more active against gra ...199910075114
evaluation of probiotic characteristics of siderophoregenic bacillus spp. isolated from dairy waste.siderophoregenic bacillus strain det9 has been selectively isolated from dairy waste. it was evaluated for probiotic characteristics and susceptibility pattern against antibiotics. its spores showed excellent tolerance to simulated gastrointestinal tract conditions and exhibited antimicrobial activity against organisms such as escherichia coli, micrococcus flavus, and staphylococcus aureus. its susceptibility to antibiotics reduces the prospect to donate resistance determinants if administered i ...201019280126
[5-fluorouracil concentration in various tissues from cancer patients after oral administration of 5-fluorouracil].5-fluorouracil(5-fu)is a widely used antitumor agent for treating patients with adenocarcinoma of gastroenterial tract and breast following intravenous or oral administration. the blood level of 5-fu peaked rapidly after intravenous bolus injection and then decreased in a short time, disappearing 90 minutes after injection. but the blood level of 5-fu peaked from 15 to 30 minutes after oral administration of 5-fu tablets or dry syrup and decreased slowly, frequently showing 0.02 microgram/ml at ...19846721512
bacterial growth in artificial capillary spaces.in experiments on the influence of microscopic capillaries on the growth of escherichia coli, brevibacterium linens, micrococcus flavus and bacillus cereus, a distinct delay in growth was observed. the difference in counts was greatest after 8 h. later a slight equilibration of counts was noted. with b. cereus, only slight or no spore formation was observed under microcapillary conditions.19846437860
a simple and convenient assay method for phosphorolysis of 5'-deoxy-5-fluorouridine.5'-deoxy-5-fluorouridine (5'-dfur) exhibits antitumor activity through its conversion to 5-fluorouracil by uridine phosphorylase (upase) in mice. this mode of activation was confirmed by the fact that a mutant strain of micrococcus flavus resistant to 5'-dfur but sensitive to 5-fluorouracil was isolated and found to be deficient in upase activity. furthermore, since the enzyme level in tumor tissue should be useful as an indicator for therapy with 5'-dfur, a simple assay method for the enzyme ac ...19816210593
in vitro antioxidant, anticholinesterase and antimicrobial activity studies on three agaricus species with fatty acid compositions and iron contents: a comparative study on the three most edible mushrooms.the fatty acids of agaricus essettei, agaricus bitorquis and agaricus bisporus were investigated by using gc and gc-ms. the dominant fatty acids were found to be linoleic (61.82-67.29%) and palmitic (12.67-14.71%) acids among the 13 fatty acids detected in the oils. total unsaturation for the oils was calculated as 77.50%, 77.44%, and 79.72%, respectively. in vitro antioxidant, anticholinesterase and antimicrobial activities were also studied. the ethyl acetate extract of agaricus bitorquis show ...201121419821
the use of chitosan, lysozyme, and the nano-silver as antimicrobial ingredients of edible protective hydrosols applied into the surface of meat.the aim of this study was to design and produce biologically active edible hydrosols, which, when applied to the surface of food products, will protect them from oxidative changes, spoilage and growth of microorganisms. verification of testing hypothesis and the degree of aim realization were performed by assessing a dpph radical scavenging activity and microbial reduction of experimental hydrosols on the basis of hydroxypropylmethylcellulose (hpmc), chitosan (ch), lysozyme (l) and nanocolloidal ...201426345019
a membrane-associated lipomannan in micrococci.membranes of micrococcus lysodeikticus, micrococcus flavus and micrococcus sodonensis contain acidic lipomannans. lipoteichoic acids could not be detected in these organisms, and the suggestion that they are substituted for by the lipomannans is strengthened by the chemical and physical resemblances between the two polymers. the mannans contain glycerol, ester-linked fatty acids and mono-esterified succinic acid residues, giving them both hydrophobic and charged properties. the m. lysodeikticus ...19751218084
comparative study of hydroalcoholic extracts of momordica charantia l. against foodborne pathogens.the antimicrobial effect of 24 different hydroalcoholic extracts (100, 75, 50 and 25% methanol and water) obtained from four parts (leaf+stem (aerial), peel, pulp and seed) of momordica charantia l. were investigated against five gram-positive, six gram-negative and four fungal strains. the extraction was done by individual cold percolation method using hexane, different hydroalcoholic solvent (100, 75, 50 and 25% methanol) and water. the antimicrobial activity was done by agar well diffusion as ...201424843188
production, purification, and characterization of micrococcin go5, a bacteriocin produced by micrococcus sp. go5 isolated from kimchi.strain go5, a bacteriocin-producing bacterium, was isolated from green onion kimchi and identified as micrococcus sp. the bacteriocin, micrococcin go5, displayed a broad spectrum of inhibitory activity against a variety of pathogenic and nonpathogenic microorganisms, as tested by the spot-on-lawn method; its activity spectrum was almost identical to that of nisin. micrococcin go5 was inactivated by trypsin (whereas nisin was not) and was completely stable at 100 degrees c for 30 min and in the p ...200515690819
resistance of gram-positive bacteria to nisin is not determined by lipid ii levels.lipid ii is essential for nisin-mediated pore formation at nano-molar concentrations. we tested whether nisin resistance could result from different lipid ii levels, by comparing the maximal lipid ii pool in micrococcus flavus (sensitive) and listeria monocytogenes (relatively insensitive) and their nisin-resistant variants, with a newly developed method. no correlation was observed between the maximal lipid ii pool and nisin sensitivity, as was further corroborated by using spheroplasts of nisi ...200415451114
antibacterial and antioxidant cassane diterpenoids from caesalpinia benthamiana.bioactivity-guided fractionation of the light petroleum extract of caesalpinia benthamiana (=mezoneuron benthamianum) root bark has led to the isolation of two cassane diterpenoids, designated as benthaminin 1 and 2. a third compound, a deoxy form of caesaldekarin c (also referred to as methyl vouacapenate) which has previously been isolated from caesalpinia major, c. bonducella, vouacapoua americana and v. macropetala, was also isolated, together with beta-sitosterol and stigmastenone. the anti ...200717418286
getting closer to the real bacterial cell wall target: biomolecular interactions of water-soluble lipid ii with glycopeptide antibiotics.a novel synthesized water-soluble variant of lipid ii (lii) was used to evaluate the noncovalent interactions between a number of glycopeptide antibiotics and their receptor by bioaffinity electrospray ionization mass spectrometry (esi-ms). the water-soluble variant of lipid ii is an improved design, compared to the traditionally used tripeptide n,n'-diacetyl-l-lysyl-d-alanyl-d-alanine (kaa), of the target molecule on the bacterial cell wall. a representative group of glycopeptide antibiotics wa ...200312658654
inorganic polyphosphate/atp-nad kinase of micrococcus flavus and mycobacterium tuberculosis h37rv.an enzyme with both inorganic polyphosphate [poly(p)]- and atp-dependent nad kinase activities was isolated from micrococcus flavus. the enzyme was a dimer consisting of 34 kda subunits, and was named poly(p)/atp-nad kinase. internal amino acid sequences of the enzyme showed homologies with some function-unknown proteins released on the genbank database. among such proteins, hypothetical rv1695 protein (accession no. z98268-16), which was encoded by a gene named "rv1695" on genomic dna of mycoba ...200011006082
influence of charge differences in the c-terminal part of nisin on antimicrobial activity and signaling capacity.three mutants of the antibiotic nisin z, in which the val32 residue was replaced by a glu, lys or trp residue, were produced and characterized for the purpose of establishing the role of charge differences in the c-terminal part of nisin on antimicrobial activity and signaling properties. 1h-nmr analyses showed that all three mutants harbor an unmodified serine residue at position 33, instead of the usual dehydroalanine. apparently, the nature of the residue preceding the serine to be dehydrated ...19979249016
fabrication of nanostructures through molecular self-assembly of small amphiphilic glyco-dehydropeptides.self-assembled peptide-based nanostructures have been the focus of research in the past decade because of their potential applications in various biological systems. normally, small self-assembled peptide nanostructures contain hydrophobic moieties, therefore, their solubility in aqueous systems poses the important challenge in the field of molecular self-assembly in order to make effective use of these in a wide variety of applications. to improve their aqueous solubility, the self-assembled am ...201222491801
lyngbyazothrins a-d, antimicrobial cyclic undecapeptides from the cultured cyanobacterium lyngbya sp.four novel cyclic undecapeptides, lyngbyazothrins a (1), b (2), c (3), and d (4), were isolated from the cultured lyngbya sp. 36.91 as binary mixtures (1/2 and 3/4). their structures were elucidated by analysis of 1d (1h and 13c) and 2d (cosy, tocsy, roesy, noesy, hmqc, and hmbc) nmr spectra, esimsms, esitofms, and amino acid analyses. three unusual amino acids were present and identified as 4-methoxyhomophenylalanine in 1 and 3, homophenylalanine in 2 and 4, and 3-amino-2,5,7,8-tetrahydroxy-10- ...200919621877
antimicrobial, cytotoxic and antioxidant activity of selected basidiomycetes from yemen.dichloromethane, methanol and aqueous extracts of 23 selected basidiomycetes species fruiting bodies collected in yemen were screened in vitro for their antibacterial activities against three gram-positive bacteria (staphyloccocus aureus, bacillus subtilis, micrococcus flavus), two gram-negative bacteria (escherichia coli, pseudomonas aeruginosa) and against one yeast fungus (candida maltosa), as well as for their cytotoxic and antioxidant activity. the highest antibacterial activity was shown b ...200516259127
mechanism of action of recombinant acc-royalisin from royal jelly of asian honeybee against gram-positive bacteria.the antibacterial activity of royalisin, an antimicrobial peptide from the royal jelly produced by honeybees, has been addressed extensively. however, its mechanism of action remains unclear. in this study, a recombinant royalisin, racc-royalisin from the royal jelly of asian honeybee apis cerana cerana, was expressed by fusing with glutathione s-transferase (gst) in escherichia coli bl21, isolated and purified. the agar dilution assays with inhibition zone showed that racc-royalisin, similar to ...201223056609
identification of a novel inhibition site in translocase mray based upon the site of interaction with lysis protein e from bacteriophage ϕx174.translocase mray is the site of action of lysis protein e from bacteriophage ϕx174. previous genetic studies have shown that mutation f288l in transmembrane helix 9 of e. coli mray confers resistance to protein e. construction of a helical wheel model for transmembrane helix 9 of mray and the transmembrane domain of protein e enabled the identification of an arg-trp-x-x-trp (rwxxw) motif in protein e that might interact with phe288 of mray and the neighbouring glu287. this motif is also found in ...201424895118
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