| conjugative transfer facilitates stable maintenance of incp-1 plasmid pkjk5 in escherichia coli cells colonizing the gastrointestinal tract of the germfree rat. | quantitative determination of incp-1 plasmid loss from escherichia coli cells colonizing the gastrointestinal tracts of germfree rats was achieved by flow cytometry. results show that the plasmid's ability to conjugate counteracts plasmid loss and is thus an important mechanism for the stable maintenance of incp-1 plasmids within the gastrointestinal environment. | 2007 | 17085707 |
| impact of conjugal transfer on the stability of incp-1 plasmid pkjk5 in bacterial populations. | the intrinsic stability of incp-1 plasmid pkjk5 was assessed in both an escherichia coli and a kluyvera sp. population maintained in bacterial mats and in liquid nutrient broth without selective pressure. a fluorescence tagging/flow cytometry approach was used to detect and quantify plasmid loss from populations harboring either conjugation-proficient or -deficient pkjk5 derivatives. the results show that the plasmid's ability to conjugate plays an important role in its stable maintenance in pop ... | 2007 | 17132149 |
| root growth and exudate production define the frequency of horizontal plasmid transfer in the rhizosphere. | to identify the main drivers of plasmid transfer in the rhizosphere, conjugal transfer was studied in the rhizospheres of pea and barley. the donor pseudomonas putida kt2442, containing plasmid pkjk5::gfp, was coated onto the seeds, while the recipient p. putida lm24, having a chromosomal insertion of dsred, was inoculated into the growth medium. mean transconjugant-to-donor ratios in vermiculite were 4.0+/-0.8 x 10(-2) in the pea and 5.9+/-1.4 x 10(-3) in the barley rhizospheres. in soil, trans ... | 2007 | 17069619 |
| effect of genomic location on horizontal transfer of a recombinant gene cassette between pseudomonas strains in the rhizosphere and spermosphere of barley seedlings. | the use of genetically engineered bacteria in natural environments constitutes a risk of transfer of recombinant dna to the indigenous bacteria. however, chromosomal genes are believed to be less likely to transfer than genes on mobilizable and conjugative plasmids. to study this assumption, horizontal transfer of a recombinant gene cassette inserted into the chromosome of a pseudomonas stutzeri strain, into a mobilizable plasmid (pagm42), and into a conjugative plasmid (pkjk5) isolated from bar ... | 2001 | 11270648 |
| the multiple antibiotic resistance incp-1 plasmid pkjk5 isolated from a soil environment is phylogenetically divergent from members of the previously established alpha, beta and delta sub-groups. | the 54,383bp plasmid pkjk5 was recovered from a soil environment by exogenous plasmid isolation and conveys resistance towards tetracycline and trimethoprim. sequencing and annotation revealed a high level of structural similarity of the backbone genes to other incp-1 plasmids containing a tra1 and tra2 region, a central control module and a replication initiation module. a considerable degree of divergence was associated with the backbone genes of pkjk5 as compared to homologous genes in the al ... | 2007 | 17306874 |