| detection of panulirus argus virus 1 (pavi) in the caribbean spiny lobster using fluorescence in situ hybridization (fish). | panulirus argus virus 1 (pav1) is the first virus known to be pathogenic to a wild lobster. it infects the caribbean spiny lobster p. argus from the florida keys, and has a predilection for juveniles. the monitoring of the virus in wild populations and study of its behavior in the laboratory require the development of reliable diagnostic tools. a sensitive and specific fluorescence in situ hybridization (fish) assay was developed for detection of pav1. the lower detection limit using a 110 bp dn ... | 2006 | 17190197 |
| detection of panulirus argus virus 1 in caribbean spiny lobsters. | panulirus argus virus 1 (pav1) is a pathogenic virus that infects caribbean spiny lobsters p. argus in the florida keys. we have developed a pcr detection assay for pav1 for the purpose of studying the natural history of the virus and for monitoring the prevalence of infection. the detection of the virus in hemolymph and other tissues is based on the pcr amplification of a 499 bp product using specific primers designed from a cloned fragment of the pav1 genome. the sensitivity limit for the assa ... | 2007 | 17718159 |
| presence of panulirus argus virus 1 (pav1) in juvenile spiny lobsters panulirus argus from the caribbean coast of mexico. | macroscopic evidence, histological sections, transmission electron microscopy (tem) evaluation, and pcr analyses of 25 apparently diseased juvenile spiny lobsters panulirus argus from the reef lagoon of puerto morelos, mexico, showed the presence of panulirus argus virus 1 (pav1). cowdry type a intranuclear viral inclusions were observed in histological analyses, icosahedral viral particles were observed by tem, and pcr using specific primers for pav1 amplified a fragment of 499 bp. this is the ... | 2008 | 18500032 |
| prevalence of panulirus argus virus 1 (pav1) and habitation patterns of healthy and diseased caribbean spiny lobsters in shelter-limited habitats. | caribbean spiny lobsters panulirus argus are socially gregarious, preferring shelters harboring conspecifics over empty shelters. in laboratory trials, however, healthy lobsters strongly avoided shelters harboring lobsters infected with the highly pathogenic panulirus argus virus 1 (pav1). because pav1 is transmitted by contact, this behavior may thwart its spread in wild lobsters. in a field experiment conducted from 1998 to 2002 in a shelter-poor reef lagoon (puerto morelos, mexico), densities ... | 2008 | 18717062 |
| diseases of spiny lobsters: a review. | spiny lobsters have few reported pathogens, parasites and symbionts. however, they do have a diverse fauna comprised of a pathogenic virus, several bacteria, protozoans, helminths and even symbiotic crustaceans. a few idiopathic syndromes have also been reported, but these appear correlated with lobsters held in poor conditions. fungal and bacterial pathogens present significant threats for rearing spiny lobsters in aquaculture settings, but only one pathogen, panulirus argus virus 1, is thought ... | 2011 | 21215357 |
| first evidence of panulirus argus virus 1 (pav1) in spiny lobster from cuba and clinical estimation of its prevalence. | the present study documents the first finding of panulirus argus virus 1 (pav1) in spiny lobster panulirus argus from cuba. samples originated from 2 nursery sites, matias keys and bocas de alonso keys, and 2 fishing sites, la grifa and el ramajo. lobsters from the nursery sites (artificial reefs) were collected by scuba diving, while those from the fishing sites were collected from artificial shelters known as 'casitas cubanas'. in these shelters it was observed that healthy lobsters tended to ... | 2011 | 21381520 |
| review of panulirus argus virus 1--a decade after its discovery. | in 2000, a pathogenic virus was discovered in juvenile caribbean spiny lobsters panulirus argus from the florida keys, u.s.a. panulirus argus virus 1 (pav1) is the first naturally occurring pathogenic virus reported from lobsters, and it profoundly affects their ecology and physiology. pav1 is widespread in the caribbean with infections reported in florida (u.s.a.), st. croix, st. kitts, yucatan (mexico), belize, and cuba. it is most prevalent and nearly always lethal in the smallest juvenile lo ... | 2011 | 21648244 |
| detection of panulirus argus virus 1 (pav1) in exported frozen tails of subadult-adult caribbean spiny lobsters panulirus argus. | the caribbean spiny lobster panulirus argus is a valuable fishing resource and the trade in frozen lobster tails is an important industry. however, the presence of the pathogenic virus panulirus argus virus 1 (pav1), which causes systemic infection in p. argus and is particularly lethal to juvenile individuals, has not been previously examined in imported/exported lobster products. we used pcr assays to determine the presence of pav1 in abdominal muscle tissue of 22 frozen p. argus tails exporte ... | 2009 | 19902844 |
| transmission of panulirus argus virus 1 (pav1) and its effect on the survival of juvenile caribbean spiny lobster. | the caribbean spiny lobster panulirus argus, an important fisheries species, is host to panulirus argus virus 1 (pav1), a lethal, unclassified virus--the first found in any species of lobster--prevalent in juvenile lobsters. we describe a series of laboratory experiments aimed at assessing the likely modes of disease transmission, determining the survival of lobsters relative to each transmission pathway and identifying potential alternate hosts. given evidence for lower prevalence of pav1 in la ... | 2008 | 18589993 |
| pathology and hematology of the caribbean spiny lobster experimentally infected with panulirus argus virus 1 (pav1). | we examined the histopathological and hematological response of the caribbean spiny lobster to experimentally induced infections with panulirus argus virus 1 (pav1). the fixed phagocytes in the hepatopancreas were the primary sites of pav1 infection in spiny lobsters. fixed phagocytes were activated in early infections. however, as the disease progressed, the fixed phagocytes became infected and eventually lysed. infected cells were subsequently observed in the hepatopancreas, gill, heart, hindg ... | 2008 | 18177971 |
| primary culture of hemocytes from the caribbean spiny lobster, panulirus argus, and their susceptibility to panulirus argus virus 1 (pav1). | primary cultures of hemocytes from the caribbean spiny lobster panulirus argus were developed for studies on the in vitro propagation of panulirus argus virus 1 (pav1). a modified leibovitz l-15 medium supported the best survival of hemocytes in in vitro primary cultures. however, degradation of the cultures occurred rapidly in the presence of granulocytes. a percoll step gradient was used to separate hemocytes into three subpopulations enriched in hyalinocytes, semigranulocytes, and granulocyte ... | 2007 | 17034814 |