| development of real-time pcr assays for the detection and differentiation of australian and european ranaviruses. | serious systemic disease in fish and amphibians is associated with the ranaviruses, epizootic haematopoietic necrosis virus (ehnv) and bohle iridovirus (biv) in australia, and european sheatfish virus (esv) and european catfish virus (ecv) in europe. ehnv, esv and ecv are recognized causative agents of the oie (office international des epizooties) notifiable systemic necrotizing iridovirus syndrome and are currently identified by protein-based assays, none of which are able to rapidly identify t ... | 2007 | 17584440 |
| susceptibility of pike esox lucius to a panel of ranavirus isolates. | in order to study the pathogenicity of ranaviruses to a wild european freshwater fish species, pike esox lucius fry were challenged with the following ranavirus isolates: epizootic haematopoietic necrosis virus (ehnv), european sheatfish virus (esv), european catfish virus (ecv), pike-perch iridovirus (ppiv), new zealand eel virus (nzeelv) and frog virus 3 (fv3). the fry were infected using bath challenge at 12 and 22 degrees c. significant mortalities were observed at 12 degrees c for ehnv, esv ... | 2009 | 19402450 |
| ranavirus phylogeny and differentiation based on major capsid protein, dna polymerase and neurofilament triplet h1-like protein genes. | in this study, we developed new methods for differentiation of ranaviruses based on polymerase chain reaction and restriction enzyme analysis of dna polymerase and neurofilament triplet h1-like (nf-h1) protein gene. using these methods, we were able to differentiate the 6 known ranaviruses--bohle iridovirus (biv), european catfish virus (ecv), epizootic haematopoietic necrosis virus (ehnv), european sheatfish virus (esv), frog virus 3 (fv3) and singapore grouper iridovirus (sgiv)--with 3 less ch ... | 2009 | 19694168 |
| susceptibility of black bullhead ameiurus melas to a panel of ranavirus isolates. | ranaviruses are considered a serious threat to lower vertebrates, including fish, amphibians and reptiles. however, epidemiological data on these agents are lacking, and further investigations are needed to understand the role of carriers and to update the list of susceptible hosts. we carried out various experimental infections under controlled conditions to contribute to the current knowledge on the susceptibility of black bullhead ameiurus melas to european catfish virus (ecv) and other ranav ... | 2010 | 20815324 |
| quantitation of ranaviruses in cell culture and tissue samples. | a quantitative real-time pcr (qpcr) based on a standard curve was developed for detection and quantitation of ranaviruses. the target gene for the qpcr was viral dna polymerase (dnapol). all ten ranavirus isolates studied (epizootic haematopoietic necrosis virus, ehnv; european catfish virus, ecv; european sheatfish virus, esv; frog virus 3, fv3; bohle iridovirus, biv; doctor fish virus, dfv; guppy virus 6, gv6; pike-perch iridovirus, ppiv; rana esculenta virus italy 282/i02, rev282/i02 and shor ... | 2010 | 21087639 |