| genetics of the glutamate-mediated methylamine utilization pathway in the facultative methylotrophic beta-proteobacterium methyloversatilis universalis fam5. | the ability of some microbial species to oxidize monomethylamine via glutamate-mediated pathways was proposed in the 1960s; however, genetic determinants of the pathways have never been described. in the present study we describe a gene cluster essential for operation of the n-methylglutamate pathway in the methylotrophic beta-proteobacterium methyloversatilis universalis fam5. four major polypeptides from protein fractions displaying high activities of n-methylglutamate synthetase, n-methylglut ... | 2010 | 19943898 |
| Use of specific gene analysis to assess the effectiveness of surfactant-enhanced trichloroethylene cometabolism. | The objective of this study was to evaluate the effectiveness of in situ bioremediation of trichloroethylene (TCE)-contaminated groundwater using specific gene analyses under the following conditions: (1) pretreatment with biodegradable surfactants [Simple Green™ (SG) and soya lecithin (SL)] to enhance TCE desorption and dissolution, and (2) supplementation with SG, SL, and cane molasses as primary substrates to enhance the aerobic cometabolism of TCE. Polymerase chain reaction (PCR), denaturing ... | 2011 | 22071259 |
| Biodegradation of melamine and its hydroxy derivatives by a bacterial consortium containing a novel Nocardioides species. | Melamine has recently been recognized as a food contaminant with adverse human health effects. Melamine contamination in some crops arises from soil and water pollution from various causes. To remove melamine from the polluted environment, a novel bacterium, Nocardioides sp. strain ATD6, capable of degrading melamine was enriched and isolated from a paddy soil sample. The enrichment culture was performed by the soil-charcoal perfusion method in the presence of triazine-degrading bacteria previou ... | 2011 | 22105542 |
| genome sequence of methyloversatilis universalisfam5t, a methylotrophic representative of the order rhodocyclales. | rhodocyclalesare representative of versatile bacteria that are able to utilize a wide variety of organic compounds for growth, but only a few strains have been isolated in pure culture thus far. here we present the genome sequence of methyloversatilis universalisfam5(t), the first cultivable methylotrophic member of the order. | 2011 | 21725020 |
| biodegradation of benazolin-ethyl by strain methyloversatilis sp. cd-1 isolated from activated sludge. | benazolin-ethyl has been used on a wide range of weeds present in various crops since 1964. because benazolin-ethyl is a potential hazard to the environment and human health, it is important to remove this herbicide from the environment. however, to the best of our knowledge, no report is available in the literature regarding the microbial degradation of benazolin-ethyl by bacteria. in this study, one strain named cd-1, which is capable of degrading benazolin-ethyl, was isolated from benazolin-e ... | 2011 | 20848105 |
| impact of varying electron donors on the molecular microbial ecology and biokinetics of methylotrophic denitrifying bacteria. | the goal of this study was to identify bacterial populations that assimilated methanol in a denitrifying sequencing batch reactor (sbr), using stable isotope probing (sip) of (13)c labeled dna and quantitatively track changes in these populations upon changing the electron donor from methanol to ethanol in the sbr feed. based on sip derived (13)c 16s rrna gene clone libraries, dominant sbr methylotrophic bacteria were related to methyloversatilis spp. and hyphomicrobium spp. these methylotrophic ... | 2009 | 19097144 |
| evaluation of performance and microbial ecology of sequencing batch reactor and membrane bioreactor treating thin-film transistor liquid crystal display wastewater. | in taiwan, a substantial amount of thin-film transistor liquid crystal display (tft-lcd) wastewater is produced daily due to an increasing production of the opto-electronic industry in recent years. the main components of tft-lcd wastewater include dimethyl sulphoxide (dmso), monoethanolamine (mea), and tetra-methyl ammonium hydroxide (tmah), which are recognized as non-or slow-biodegradable organic compounds and limited information is available regarding their biological treatablility. this stu ... | 2008 | 18824808 |
| molecular and biokinetic characterization of methylotrophic denitrification using nitrate and nitrite as terminal electron acceptors. | although methanol is a widely employed carbon source for denitrification, relatively little is known on the abundance and diversity of methylotrophic bacteria in activated sludge. the primary aim of this study was to specifically identify bacteria that metabolized methanol in a sequencing batch denitrifying reactor (sbdr), using a novel technique, stable isotope probing (sip) of 13c labeled dna. a secondary aim was to quantitatively track dominant methylotrophic bacteria in the sbdr exposed to d ... | 2008 | 18701786 |
| characterization of a novel methanol dehydrogenase in representatives of burkholderiales: implications for environmental detection of methylotrophy and evidence for convergent evolution. | some members of burkholderiales are able to grow on methanol but lack the genes (mxafi) responsible for the well-characterized two-subunit pyrroloquinoline quinone-dependent quinoprotein methanol dehydrogenase that is widespread in methylotrophic proteobacteria. here, we characterized novel, mono-subunit enzymes responsible for methanol oxidation in four strains, methyloversatilis universalis fam5, methylibium petroleiphilum pm1, and unclassified burkholderiales strains rz18-153 and fam1. the en ... | 2008 | 18390659 |
| methyloversatilis universalis gen. nov., sp. nov., a novel taxon within the betaproteobacteria represented by three methylotrophic isolates. | the taxonomic positions and phylogenetic relationships of two new methylotrophic isolates from lake washington (usa) sediment, fam5t and 500, and the previously described methylotrophic strain ehg5 isolated from contaminated soil in estarreja (portugal) were investigated. all three strains were facultative methylotrophs capable of growth on a variety of c1 and multicarbon compounds. optimal growth occurred at ph 7.5-8 and 30-37 degrees c. the major fatty acids were c16:1omega7c and c16:0. the ma ... | 2006 | 17082383 |