identification of ehrlichia chaffeensis by nested pcr in ticks from southern china.a total of 717 ticks collected from southern china were examined by nested pcr for the presence of ehrlichia chaffeensis. sixteen (55. 2%) of 29 adult amblyomma testudinarium ticks and 28 (11.7%) of 240 adult and at least 4.2% of 215 nymphal (pooled specimens) haemaphysalis yeni ticks tested positive. four other species of ticks were negative. selected positive amplicons were confirmed by dna sequencing.200010878087
genetic identification of rickettsiae isolated from ticks in japan.following the description in japan of japanese spotted fever, caused by rickettsia japonica, a search for the vector of this disease led to the isolation of several rickettsiae from various tick species. sixty-three rickettsial isolates were obtained from six different tick species, and six type strains were described by pcr and monoclonal antibody testing. we identified these six strains by amplification and sequencing of the genes encoding 16s rrna and citrate synthase. we confirmed that the i ...200212037083
ehrlichia chaffeensis: a prototypical emerging pathogen.ehrlichia chaffeensis is an obligately intracellular, tick-transmitted bacterium that is maintained in nature in a cycle involving at least one and perhaps several vertebrate reservoir hosts. the moderate to severe disease caused by e. chaffeensis in humans, first identified in 1986 and reported for more than 1,000 patients through 2000, represents a prototypical "emerging infection." knowledge of the biology and natural history of e. chaffeensis, and of the epidemiology, clinical features, and ...200312525424
detection of ehrlichia spp., anaplasma spp., rickettsia spp., and other eubacteria in ticks from the thai-myanmar border and vietnam.a total of 650 ticks, including 13 species from five genera, were collected from animals, from people, or by flagging of the vegetation at sites on the thai-myanmar border and in vietnam. they were tested by pcr to detect dna of bacteria of the order rickettsiales: three anaplasma spp. were detected in ticks collected in thailand, including (i) anaplasma sp. strain anda465, which was considered a genotype of anaplasma platys (formerly ehrlichia platys) and which was obtained from dermacentor aur ...200312682151
molecular evidence for novel tick-associated spotted fever group rickettsiae from thailand.ticks are of considerable medical and veterinary importance because they directly harm the host through their feeding action and indirectly through vectoring many bacterial pathogens. despite many ticks being known from thailand, very little is known about the bacteria they may harbor. we report here the results of a survey of tick-associated bacteria in thailand. a total of 334 individuals representing 14 species of ticks in five genera were collected from 10 locations in thailand and were exam ...200312693853
ehrlichiae and ehrlichial diseases in china.the various ticks collected from different areas of china were examined for the existence of ehrlichial agents by polymerase chain reaction (pcr) with genus- or species-specific primers designed on the basis of ehrlichial 16s rrna genes and sequence analyses. in southern china, e. chaffeensis was detected in amblyomma testudinarium ticks from infested cattle, haemaphysalis yeni ticks from hare, and ixodes ovatus ticks from muntiacus reevesi. e. canis was identified in rhipicephalus sanguineus ti ...200312860598
detection of antibodies to anaplasma phagocytophilum and ehrlichia chaffeensis antigens in sera of korean patients by western immunoblotting and indirect immunofluorescence assays.two hundred seventy one serum samples from south korean patients were tested to detect antibodies against anaplasma phagocytophilum (the human granulocytic ehrlichiosis agent) and ehrlichia chaffeensis (the human monocytic ehrlichiosis agent) by indirect fluorescent-antibody assay (ifa) and the western blot assay. these sera were collected from patients with symptoms of high fever. the rate of seropositivity for orientia tsutsugamushi was 50.9% by ifa at the public health & environmental researc ...200314607867
tick-borne rickettsioses around the world: emerging diseases challenging old concepts.during most of the 20th century, the epidemiology of tick-borne rickettsioses could be summarized as the occurrence of a single pathogenic rickettsia on each continent. an element of this paradigm suggested that the many other characterized and noncharacterized rickettsiae isolated from ticks were not pathogenic to humans. in this context, it was considered that relatively few tick-borne rickettsiae caused human disease. this concept was modified extensively from 1984 through 2005 by the identif ...200516223955
an investigation into the distribution, host-preference and population density of ixodid ticks affecting domestic animals in study the distribution, host-preference and population density of ixodid ticks in bangladesh, an attempt was made to collect adult ticks from various host animals in three distinct topographic zones, viz. flood plains, hills and steppe 'barind'. five species of ixodid ticks were recorded, namely, boophilus microplus (56.3%), haemaphysalis bispinosa (11.3%), rhipicephalus sanguineus (14.7%), hyalomma anatolicum anatolicum (15.0%) and amblyomma testudinarium (2.8%). the data showed that b. micr ...200617243476
diagnostic assay for rickettsia japonica.we developed a specific and rapid detection system for rickettsia japonica and r. heilongjiangensis, the causative agents of spotted fever, using a taqman minor groove binder probe for a particular open reading frame (orf) identified by the r. japonica genome project. the target orf was present only in r. japonica-related strains.200919961684
human ehrlichiosis and anaplasmosis.human ehrlichiosis and anaplasmosis are acute febrile tick-borne diseases caused by various members of the genera ehrlichia and anaplasma (anaplasmataceae). human monocytotropic ehrlichiosis has become one of the most prevalent life-threatening tick-borne disease in the united states. ehrlichiosis and anaplasmosis are becoming more frequently diagnosed as the cause of human infections, as animal reservoirs and tick vectors have increased in number and humans have inhabited areas where reservoir ...201020513551
the diagnostic usefulness of dermoscopy for identifying six-legged larval ticks.ticks are vectors of a variety of diseases such as lyme disease and japanese spotted fever. we examined an 87-year-old female with multiple tick bites by at least 236 larval amblyomma testudinarium infestations. numerous tick bites are generally caused by the six-legged larvae, which were verified in this case by dermoscopy. the present case indicates the diagnostic usefulness of dermoscopy for six-legged larval tick bites.201021771529
a novel approach, based on blsoms (batch learning self-organizing maps), to the microbiome analysis of ticks.ticks transmit a variety of viral, bacterial and protozoal pathogens, which are often zoonotic. the aim of this study was to identify diverse tick microbiomes, which may contain as-yet unidentified pathogens, using a metagenomic approach. dna prepared from bacteria/archaea-enriched fractions obtained from seven tick species, namely amblyomma testudinarium, amblyomma variegatum, haemaphysalis formosensis, haemaphysalis longicornis, ixodes ovatus, ixodes persulcatus and ixodes ricinus, was subject ...201323303373
emerging tick-borne infections in mainland china: an increasing public health threat.since the beginning of the 1980s, 33 emerging tick-borne agents have been identified in mainland china, including eight species of spotted fever group rickettsiae, seven species in the family anaplasmataceae, six genospecies in the complex borrelia burgdorferi sensu lato, 11 species of babesia, and the virus causing severe fever with thrombocytopenia syndrome. in this review we have mapped the geographical distributions of human cases of infection. 15 of the 33 emerging tick-borne agents have be ...201526453241
modulation of host immunity by tick generation sequencing and proteomics have helped to comprehensively characterize gene expression in tick salivary glands at both the transcriptome and the proteome level. functional data are, however, lacking. given that tick salivary secretions are critical to the success of the tick transmission lifecycle and, as a consequence, for host colonization by the pathogens they spread, we thoroughly review here the literature on the known interactions between tick saliva (or tick salivary gland ...201526189360
prevalence of borrelia burgdorferi sensu lato in ticks from eastern explore the tick distribution and prevalence of borrelia in zhejiang province, we performed a survey in nine sites. a total of 447 adult ticks of 11 species were captured and the dominant tick species were haemaphysalis longicornis and ixodes sinensis and the abundance of tick species in different areas varied significantly. overall, 4.70% of the ticks were polymerase chain reaction (pcr) positive for borrelia. the average pcr positive rates were 5.19% for h. longicornis, 3.45% for amblyomma ...201525548382
investigation of tick-borne bacteria (rickettsia spp., anaplasma spp., ehrlichia spp. and borrelia spp.) in ticks collected from andean tapirs, cattle and vegetation from a protected area in ecuador.ixodid ticks play an important role in the transmission and ecology of infectious diseases. information about the circulation of tick-borne bacteria in ticks is lacking in ecuador. our aims were to investigate the tick species that parasitize andean tapirs and cattle, and those present in the vegetation from the buffer zone of the antisana ecological reserve and cayambe-coca national park (ecuador), and to investigate the presence of tick-borne bacteria.201525616567
tick bite by nymphal amblyomma testudinarium.ticks are parasites that usually suck the blood of wild or domestic animals; rarely, they ingest human blood and spread various febrile infectious diseases along with skin problems. out of 40 cases of tick bite reported in korea, only 3 were caused by nymphal ticks, and tick bites by nymphal amblyomma testudinarium have not been reported previously. herein, we report a rare case of tick bite by nymphal a. testudinarium. a 57-year-old woman presented with an asymptomatic solitary erythematous nod ...201627904278
molecular detection and characterization of zoonotic and veterinary pathogens in ticks from northeastern china.tick-borne diseases are considered as emerging infectious diseases in humans and animals in china. in this study, ixodes persulcatus (n = 1699), haemaphysalis concinna (n = 412), haemaphysalis longicornis (n = 390), dermacentor nuttalli (n = 253), and dermacentor silvarum (n = 204) ticks were collected by flagging from northeastern china, and detected for infection with anaplasma, ehrlichia, babesia, and hepatozoon spp. by using nested polymerase chain reaction assays and sequencing analysis. an ...201627965644
update on tick-borne rickettsioses around the world: a geographic approach.tick-borne rickettsioses are caused by obligate intracellular bacteria belonging to the spotted fever group of the genus rickettsia. these zoonoses are among the oldest known vector-borne diseases. however, in the past 25 years, the scope and importance of the recognized tick-associated rickettsial pathogens have increased dramatically, making this complex of diseases an ideal paradigm for the understanding of emerging and reemerging infections. several species of tick-borne rickettsiae that wer ...024092850
ecology of the tick-borne phlebovirus causing severe fever with thrombocytopenia syndrome in an endemic area of china.severe fever with thrombocytopenia syndrome (sfts) is caused by sfts virus (sftsv), a tick-borne phlebovirus in family bunyaviridae. studies have found that humans, domestic and wildlife animals can be infected by sftsv. however, the viral ecology, circulation, and transmission remain largely unknown.201627035712
isolation of the rickettsial agent genetically similar to candidatus rickettsia kotlanii, from haemaphysalis megaspinosa in japan.two rickettsial isolates, hm-1 and hm-2, were isolated from haemaphysalis megaspinosa collected in japan in 2006 and 2011, respectively. the isolates were analyzed by dna sequences of the outer membrane protein a gene, the outer membrane protein b gene, the citrate synthase gene, the genus rickettsia-specific outer membrane protein 17-kda gene, the 16s ribosome rna gene, and the ps120 protein gene ("gened"). hm-1 was identified as rickettsia tamurae. hm-2 matched most closely with 'candidatus ri ...025229707
ticks collected from wild and domestic animals and natural habitats in the republic of korea.ticks were collected from 35 animals from 5 provinces and 3 metropolitan cities during 2012. ticks also were collected by tick drag from 4 sites in gyeonggi-do (2) and jeollabuk-do (2) provinces. a total of 612 ticks belonging to 6 species and 3 genera were collected from mammals and a bird (n=573) and by tick drag (n=39). haemaphyalis longicornis (n=434) was the most commonly collected tick, followed by h. flava (158), ixodes nipponensis (11), amblyomma testudinarium (7), h. japonica (1), and h ...201425031468
a case of amblyomma testudinarium tick bite in a korean woman.a case of tick bite was found in the inguinal region of a 74-year-old korean woman. she was attacked by the tick while working in her vegetable garden in the vicinity of mountain located in suncheon city, the southern coastal area of the korean peninsula. on admission she complained of mild discomfort and itching around the bite area. the causative tick was 23 mm long and had slender pedipalps. the scutum was quite ornate and had eyes at the edge. the genital aperture was located anterior to the ...201021234234
acarine ectoparasites of panti forest reserve in johore, identify the presence of acarine ectoparasites and determine whether there is any potential public health risk in panti forest reserve, johore, malaysia.023569714
large-scale survey for tickborne bacteria, khammouan province, laos.we screened 768 tick pools containing 6,962 ticks from khammouan province, laos, by using quantitative real-time pcr and identified rickettsia spp., ehrlichia spp., and borrelia spp. sequencing of rickettsia spp.-positive and borrelia spp.-positive pools provided evidence for distinct genotypes. our results identified bacteria with human disease potential in ticks in laos.027532491
tick infestation of sika deer (cervus nippon) in the western part of yamaguchi prefecture, japan.ticks were collected from 94 sika deer (cervus nippon) hunted in the western part of yamaguchi prefecture, japan from august to november 1999, and march to july 2000. haemaphysalis longicornis and h. yeni were the dominant species from april to august, while h. flava and h. megaspinosa were dominant in october, november and march. this is the first report of h. yeni in the mainland of japan. small numbers of h. kitaokai, amblyomma testudinarium and ixodes ovatus were also recorded.200212185317
detection of rickettsia japonica in haemaphysalis longicornis ticks by restriction fragment length polymorphism of pcr product.pcr was applied to the detection of rickettsia japonica, the causative agent of oriental spotted fever (osf), in ticks collected at two sites of the muroto area on shikoku island, a major area in japan where osf is endemic. primer pair rr190.70p and rr190.602n of the r. rickettsii 190-kda antigen gene sequence of regnery and others (r.l. regnery, c.l. spruill, and b.d. plikaytis, j. bacteriol. 173:1576-1589, 1991) primed the dna extracted from haemaphysalis longicornis ticks but not those extrac ...19957790445
detection of rickettsial dna in ticks and wild boars in kyoto city, japan.the tick is a well-known vector for arthropod-borne pathogens, such as tick-borne encephalitis, lyme disease, japanese spotted fever and severe fever with thrombocytopenia syndrome. it is therefore important to know the tick population and distribution in our environment and wild animals in order to prevent tick-borne diseases. here, we report the results of tick surveillance from may to september 2011 at 14 geographical points and in 5 wild boars in kyoto city, kyoto prefecture, japan. we colle ...201425298315
first isolation of severe fever with thrombocytopenia syndrome virus from haemaphysalis longicornis ticks collected in severe fever with thrombocytopenia syndrome outbreak areas in the republic of korea.severe fever with thrombocytopenia syndrome (sfts) is an emerging tick-borne infectious disease that is endemic to china, japan, and the republic of korea (rok). in this study, 8313 ticks collected from sfts outbreak areas in the rok in 2013 were used to detect the sfts virus (sftsv). a single sftsv was isolated in cell culture from one pool of haemaphysalis longicornis ticks collected from samcheok-si, gangwon province, in the rok. phylogenetic analysis showed that the sftsv isolate was cluster ...201626745758
severe fever with thrombocytopenia syndrome virus in ticks collected from humans, south korea, 2013.we investigated the infection rate for severe fever with thrombocytopenia syndrome virus (sftsv) among ticks collected from humans during may-october 2013 in south korea. haemaphysalis longicornis ticks have been considered the sftsv vector. however, we detected the virus in h. longicornis, amblyomma testudinarium, and ixodes nipponensis ticks, indicating additional potential sftsv vectors.025061851
human granulocytic anaplasmosis, japan.we retrospectively confirmed 2 cases of human anaplasma phagocytophilum infection. patient blood samples contained unique p44/msp2 for the pathogen, and antibodies bound to a. phagocytophilum antigens propagated in thp-1 rather than hl60 cells. unless both cell lines are used for serodiagnosis of rickettsiosis-like infections, cases of human granulocytic anaplasmosis could go undetected.023460988
dermoscopy for tick bite: reconfirmation of the usefulness for the initial diagnosis.ticks transmit a variety of infectious diseases. diagnosis requires verification of a tick's presence. here, we describe a 61-year-old woman bitten by an eight-legged nymphal amblyomma testudinarium. we re-emphasize the usefulness of dermoscopy for identifying signs of the bite and determining the species of the biting tick.201121577370
molecular detection and identification of piroplasms in sika deer (cervus nippon) from jilin province, china.piroplasmosis is an important disease of domestic animals and wildlife and is caused by organisms from the genera theileria and babesia. wildlife such as sika deer play an important role as reservoir hosts for several species of theileria and babesia. using blood samples collected from sika deer, we investigated the epidemiology of theileria spp. and babesia spp. in sika deer from jilin province in china and identified those species that cause pathogenic infections in sika deer.201626984286
cross-sectional survey of ixodid tick species on grazing cattle in japan.ixodid tick species were collected from cattle in 60 grazing fields throughout japan. haemaphysalis longicornis was mainly recovered in the western and southern regions, while ixodes species were collected mainly in the central to northern regions. other tick species such as amblyomma testudinarium, boophilus microplus, h. flava and h. kitaokai were identified from a few fields in the central and southern regions. haemaphysalis longicornis were recovered in the fields with higher temperatures an ...200616550336
[random amplified polymorphic dna analysis of the genomes among 7 species of ticks].to study genomic polymorphic dna and genetic distance of 7 species of ticks.200415587156
survey of ixodid tick species on domestic cats in japan.various species of ixodid ticks, attached to domestic cats in japan, were identified in spring (april to june) and autumn (september to november). in the spring, a total of 282 ticks, including 61 larvae, 70 nymphs, 127 females and 24 males were collected from 126 cats. of these, 264 were identified up to the species level. in the spring, haemaphysalis longicornis was the most frequently (39.7%, 50/126) found tick species on feline hosts, followed by ixodes ovatus (35.0%, 44/126), ixodes nippone ...200312531297
perianal tick-bite lesion caused by a fully engorged female amblyomma testudinarium.a perianal tick and the surrounding skin were surgically excised from a 73-year-old man residing in a southwestern costal area of the korean peninsula. microscopically a deep penetrating lesion was formed beneath the attachment site. dense and mixed inflammatory cell infiltrations occurred in the dermis and subcutaneous tissues around the feeding lesion. amorphous eosinophilic cement was abundant in the center of the lesion. the tick had y-shaped anal groove, long mouthparts, ornate scutum, comm ...201425548423
first records of tick-borne pathogens, borrelia, and spotted fever group rickettsiae in okinawajima island, early april 2000, tick-borne pathogens were surveyed in the northern area of okinawajima island, okinawa prefecture, which is the southernmost area of japan. borrelia valaisiana, a lyme disease spirochete, was isolated from a field mouse mus calori, and unidentified rickettsiae of the spotted fever group were isolated from all stages of amblyomma testudinarium. these are the first reports of these pathogens on okinawajima island.200111293483
detection of spotted fever group rickettsiae in ticks from zhejiang province, china.tick species distribution and prevalence of spotted fever group rickettsiae (sfgr) in ticks were investigated in zhejiang province, china in 2010 and 2011. pcr was used to detect sfgr and positive amplicons were sequenced, compared to published sequences and phylogenic analysis was performed using mega 4.0. a total of 292 adult ticks of ten species were captured and 7.5 % (22/292) of the ticks were pcr-positive for sfg rickettsia. the pcr-positive rates were 5.5 % (6/110) for haemaphysalis longi ...201525633265
the first human case of rickettsia tamurae infection in japan.a case of rickettsia tamurae infection in japan is reported. a 76-year-old japanese male had a tick bite which developed to local skin inflammation on his left leg. anti-rickettsia antibodies were detected in his serum, and r. tamurae dna was identified in his blood, the lesional skin, and the tick.201121503163
rickettsia tamurae sp. nov., isolated from amblyomma testudinarium ticks.rickettsia sp. strain at-1(t) was isolated from amblyomma testudinarium ticks in japan in 1993. comparative analysis of sequences obtained from 16s rrna, glta, ompa, ompb and sca4 gene fragments demonstrated those from at-1(t) to be markedly different from those of other members of the spotted fever group. using mouse serotyping, it was also observed that rickettsia sp. strain at-1(t) was different from other rickettsia species with validly published names. such genotypic and phenotypic characte ...200616825648
ixodid tick species recovered from domestic dogs in japan.the species of ixodid ticks (acari: ixodidae) recovered from domestic dogs in japan between september to november 2000 and april to june 2001 were identified. a total of 4122 ticks, including 1624 larvae, 1200 nymphs, 1016 females and 282 males were removed from 1221 dogs during these periods. haemaphysalis longicornis (neumann) was the most frequently found (40.3% of dogs), followed by h. flava (neumann) (16.1% of dogs), rhipicephalus sanguineus (latreille) (4.8% of dogs) and ixodes ovatus (neu ...200312680923
detection of sfts virus in ixodes nipponensis and amblyomma testudinarium (ixodida: ixodidae) collected from reptiles in the republic of korea.a survey of reptile-associated ticks and their infection status with severe fever with thrombocytopenia syndrome (sfts) virus was conducted to determine the relative abundance and distribution among lizards, skinks, and snakes in the republic of korea (rok). in total, 132 reptiles, including 49 lizards (two species), 15 skinks (one species), and 68 snakes (eight species) were collected. in total, 84 ixodid ticks belonging to two genera (ixodes and amblyomma) were collected from 28/132 (21.2%) li ...201626957392
case of tick-associated rash illness caused by amblyomma testudinarium.we report a case of tick-associated rash illness (tari), a new clinical entity of erythema migrans associated with a tick bite without infection of lyme borreliosis agent. the patient, a 53-year-old man, went hiking in a mountainous area of minoh city, osaka prefecture in october 2012. an erythematous macule with itching and a biting tick was found on his left thigh 2 days later, which gradually expanded. on the first visit to our department at the fifth day after hiking, an erythematous macule ...201425156744
ticks parasitising the indian buffalo (bubalus bubalis) and their possible role in disease transmission.a total of 13 ixodid tick species, boophilus microplus, haemaphysalis bispinosa, haemaphysalis cornupunctata, haemaphysalis himalaya, heamaphysalis montgomeryi, hyalomma anatolicum anatolicum, hyalomma dromedarii, hyalomma marginatum isaaci, hyalomma (hyalommina) brevipunctata, hyalomma (hyalommina) hussaini, nosomma monstrosum, rhipicephalus haemaphysaloides, and r. turanicus were collected off 424 buffaloes from the northwestern states of india. ten tick species, amblyomma testudinarium, b. mi ...19883369083
epidemiological and clinical features of severe fever with thrombocytopenia syndrome in japan, 2013-2014.although severe fever with thrombocytopenia syndrome (sfts) was first reported from japan in 2013, the precise clinical features and the risk factors for sfts have not been fully investigated in japan. ninety-six cases of severe fever with thrombocytopenia syndrome (sfts) were notified through the national surveillance system between april 2013 and september 2014 in japan. all cases were from western japan, and 82 cases (85%) had an onset between april and august. a retrospective observational s ...201627776187
haemaphysalis longicornis ticks as reservoir and vector of severe fever with thrombocytopenia syndrome virus in china.severe fever with thrombocytopenia syndrome (sfts) is an emerging hemorrhagic fever in east asia caused by sfts virus (sftsv), a newly discovered phlebovirus. the haemaphysalis longicornis tick has been suspected to be the vector of sftsv. to determine whether sftsv can be transmitted among ticks, from ticks to animals, and from animals to ticks, we conducted transmission studies between developmental stages of h. longicornis ticks and between ticks and mice. using reverse transcription pcr, we ...026402039
case of tick bite with infestation of an extraordinary number of larval amblyomma testudinarium ticks. 201121434986
severe fever with thrombocytopenia syndrome in japan and public health communication.a fatal case of severe fever with thrombocytopenia syndrome was reported in japan in 2013. the ensuing process of public communication offers lessons on how to balance public health needs with patient privacy and highlights the importance of multilateral collaborations between scientific and political communities.025695132
report of amblyomma testudinarium in mithuns (bos frontalis) from eastern mizoram (india).physical examination of semi-domesticated, free ranging mithuns (bos frontalis) during an animal health check-up and treatment camp organized at khuwangleng village in the champhai district of mizoram, india and adjacent to myanmar border revealed presence of unusually large blood engorged ticks attaching to the dewlap and inner aspects of thighs. on the basis of morphological study, the ticks were found indistinguishable from female amblyomma testudinarium. prevalence rate of the tick species i ...201627876918
genome sequence of rickettsia tamurae, a recently detected human pathogen in japan.rickettsia tamurae is a member of the spotted fever group rickettsiae, which was reported in 2011 to cause human infections in japan. we report the draft genome sequence of r. tamurae strain at-1(t), isolated from amblyomma testudinarium ticks.201425189579
rickettsial infections in southeast asia: implications for local populace and febrile returned travelers.rickettsial infections represent a major cause of non-malarial febrile illnesses among the residents of southeast asia and returned travelers from that region. there are several challenges in recognition, diagnosis, and management of rickettsioses endemic to southeast asia. this review focuses on the prevalent rickettsial infections, namely, murine typhus (rickettsia typhi), scrub typhus (orientia tsutsugamushi), and members of spotted fever group rickettsiae. information on epidemiology and reg ...201424957537
tick bite on glans penis: the role of dermoscopy. 201324371416
molecular survey of arthropod-borne pathogens in ticks obtained from japanese wildcats.the iriomote cat (ic), prionailurus bengalensis iriomotensis, and the tsushima leopard cat (tlc), prionailurus bengalensis euptilurus, are endangered subspecies of leopard cats in japan. in addition to habitat destruction and road kills, infectious diseases may threaten their populations, and infection with arthropod-borne pathogens has been reported in both subspecies. infestations with ectoparasites, especially ticks, have frequently been observed in ics and tlcs. in the present study, ticks c ...201525682495
comparative observations on some bionomics of japanese ixodid ticks under laboratory cultural conditions.seventeen species of japanese ixodid ticks of the genera amblyomma, boophilus, haemaphysalis, ixodes, and rhipicephalus were reared in the laboratory under almost the same cultural conditions. their major biological characters, such as feeding, molting, oviposition, and hatching, were summarized in tables. there were some differences in these bionomics among the species. the general characteristics common to all the ticks, except amblyomma testudinarium, were as follows. the feeding period in im ...1976980121
accumulating process and concentration ratios of ingested blood meals in larvae and nymphs of ten species of ticks.the accumulating process and concentration ratios of ingested blood meals in the larvae and nymphs of amblyomma testudinarium, haemaphysalis campanulata, h. concinna, h. formosensis, h. hystricis, h. kitaokai, the bisexual and parthenogenetic strains of h. longicornis, h. megaspinosa, and ixodes persulcatus on rabbits and argas japonicus on chickens were comparatively investigated. the larvae and nymphs of the ixodid ticks examined and the larvae of a. japonicus concentrated the ingested blood m ...1976980120
prospects of using dna barcoding for species identification and evaluation of the accuracy of sequence databases for ticks (acari: ixodida).ticks are important vectors of disease and parasites of livestock. species identification of ticks has been traditionally based on morphological characters, which is usually limited by the condition of samples and little variation among specimens, so a rapid and reliable identification method is needed. dna barcoding uses a standard fragment of the mitochondrial gene cytochrome oxidase c subunit i (coi) to identify species and has been successfully used in many taxa. in this study, we applied dn ...201424656809
ticks (acari: ixodidae) from medium-sized to large mammals in ehime prefecture, japan.ixodid ticks were collected from medium-sized to large mammals in ehime prefecture, shikoku, japan. ten species of ticks (amblyomma testudinarium, dermacentor taiwanensis, haemaphysalis flava, h. formosensis, h. hystricis, h. longicornis, h. megaspinosa, ixodes nipponensis, i. ovatus, and i. tanuki) were collected from a total of 29 mammals comprising 11 species. haemaphysalis hystricis, a possible vector of japanese spotted fever in ehime prefecture, was collected from canis lupus familiaris (d ...201323242932
identification of a complex peptidergic neuroendocrine network in the hard tick, rhipicephalus appendiculatus.neuropeptides are crucial regulators of development and various physiological functions but little is known about their identity, expression and function in vectors of pathogens causing serious diseases, such as ticks. therefore, we have used antibodies against multiple insect and crustacean neuropeptides to reveal the presence of these bioactive molecules in peptidergic neurons and cells of the ixodid tick rhipicephalus appendiculatus. these antibodies have detected 15 different immunoreactive ...200919082627
ticks (acari: ixodidae) parasitizing wild carnivores in phu khieo wildlife sanctuary, thailand.ixodid ticks were collected and identified from 8 wild carnivore species in phu khieo wildlife sanctuary, northeastern thailand. six tick species belonging to 4 genera were recovered and identified from 132 individuals. these included amblyomma testudinarium (n = 36), haemaphysalis asiatica (n = 58), h. hystricis (n = 31), h. semermis (n = 3), rhipicephalus haemaphysaloides (n = 3), and ixodes granulatus (n = 1). leopard cats (prionailurus bengalensis) (n = 19) were infested with 4 tick species, ...200415270119
a nationwide survey of ixodid tick species recovered from domestic dogs and cats in japan in 2011.a nationwide survey of ixodid ticks was performed in 2011, during which a total of 4237 and 298 ticks were recovered from 1162 dogs and 136 cats, respectively. haemaphysalis longicornis was the most frequently found tick species on canine hosts (739 dogs), followed by h. flava (166), ixodes ovatus (139), and rhipicephalus sanguineus sensu lato (70). h. hystricis, h. japonica, h. megaspinosa, h. formosensis, h. campanulata, h. ias, i. nipponensis, i. persulcatus, and amblyomma testudinarium were ...201425155339
amblyomma testudinarium tick bite: one case of engorged adult and a case of extraordinary number of larval tick infestation.this paper reports two recent cases of tick bite due to amblyomma testudinarium. the first case was an 86-year-old farmer infested with a fully engorged adult tick attached on his inguinal region. the second case was a 57-year-old male infested with an extraordinarily large number of larval ticks (> 100 larvae). the ticks were identified as a. testudinarium based on morphological characteristics. to our knowledge, the latter case is the eleventh case of larval tick bites among all tick species a ...200011211793
a case of tick bite from amblyomma testudinarium on the glans penis.a tick which bit the glans penis of a 40-year-old japanese man was surgically excised together with the skin where the bite occurred. it was identified as amblyomma testudinarium through the taxonomical investigation of its morphological characteristics. to our best knowledge, our patient is the sixth tick bite from this species of tick recorded in the chugoku district of japan.19968839244
detachment of hard ticks (acari: ixodidae) from hunted sika deer (cervus nippon).ixodid ticks were collected from 13 sika deer, cervus n.nippon, shot in the boso peninsula in central japan from late february to early march 1999. haemaphysalis megaspinosa was the most abundant species of the adults collected, although haemaphysalis longicornis, h. flava, h. kitaokai, h. cornigera, ixodes ovatus, and amblyomma testudinarium were also collected. males were more abundant than females for h. longicornis, h. megaspinosa, h. flava, and h. kitaokai. ticks that had inserted their hyp ...201424659518
prevalence of tick-borne encephalitis virus in ticks from southern korea.the prevalence of tick-borne encephalitis virus (tbev) in southern korea was determined by collecting ticks using tick drags. a total of 4,077 of 6,788 ticks collected were pooled (649 pools) according to collection site, species, and developmental stage and assayed for tbev. the tbev protein e and ns5 gene fragments were detected using rt-nested pcr in six pools of nymphs collected from jeju island (2,491 ticks). the minimum field detection rates for tbev were 0.17% and 0.14% for haemaphysalis ...201020706026
an opioid peptide from synganglia of the tick, amblyomma opioid peptide, which shares similarity with mammalian hemorphins, has been identified from the synganglia (central nervous system) of the hard tick, amblyomma testindiarium. its primary sequence was established as lvvypwtkm that contains a tetrapeptide sequence tyr-pro-trp-thr of hemorphin-like opioid peptides. by hot-plate bioassay, the purified peptide and synthetic peptide displayed dose-related antinociceptive effect in mice, as observed for other hemorphin-like opioid peptides. this is ...200415752574
phylogenetic analysis of spotted fever group rickettsiae isolated from ticks in japan.eight spotted fever group (sfg) rickettsiae isolated from ticks in japan were classified by phylogenetic analysis based on the nucleotide sequences of both the citrate synthase-encoding gene (glta) and 190-kda antigen-encoding gene (rompa). in the phylogenetic tree of glta, strains dt-1 and fla-1 isolated from the dermacentor taiwanensis and haemaphysalis frava ticks, respectively, were placed as rickettsia japonica, and strains io-1, io-2, io-25, im-1 and ip-2 from genus ixodes ticks were place ...200212061626
identification of rickettsial isolates at the species level using multi-spacer order to estimate whether multi-spacer typing (mst), based on the sequencing of variable intergenic spacers, could serve for the identification of rickettsia at the species level, we applied it to 108 rickettsial isolates or arthropod amplicons that include representatives of 23 valid rickettsia species.200717662158
surveillance of ticks and associated pathogens in free-ranging formosan pangolins (manis pentadactyla pentadactyla).chinese pangolins are critically endangered insectivorous mammals distributed in several south and southeast asian countries. in recent years, there has been an increase in spread of tick-borne diseases in both humans and animals worldwide. currently, limited information is available on ticks and associated pathogens infesting pangolins. the objective of the present study was to survey ticks and associated pathogens in the formosan pangolin population in southeastern taiwan. free-ranging formosa ...201627426438
repeated amblyomma testudinarium tick bites are associated with elevated anti-galactose-α-1,3- galactose carbohydrate ige antibody levels: a retrospective cohort study in a single institute.alpha gal syndrome is a hypersensitivity reaction to red meat mediated by specific ige antibody to galactose-α-1,3-galactose carbohydrate (alpha gal). amblyomma tick bites are associated with this condition although the pathophysiology is not understood.201729273488
francisella-like endosymbiont detected in haemaphysalis ticks (acari: ixodidae) from the republic of korea.a total of 6,255 ticks belonging to three genera and six species (haemaphysalis flava neumann, haemaphysalis longicornis neumann, haemaphysalis phasiana saito, ixodes nipponensis kitaoka & saito, ixodes persulcatus schulze, and amblyomma testudinarium koch) collected from may-august, 2013, at four southwestern provinces in the republic of korea (rok) were submitted to the armed forces research institute of medical sciences and assayed for selected tick-borne pathogens. one pool each of h. flava ...201728981682
human otoacariasis caused by amblyomma testudinarium: diagnosis and management: case report.tick infestation of the external auditory canal (eac) constitutes <1% of all patients presenting with ear complaints. consequently, parameters for the diagnosis and management of ticks in the eac have not been established.201728658173
identification of tick species collected from wild boars and habitats of wild boars and domestic pigs in the republic of korea.tick is one of the most important arthropods in the transmission of vector-borne diseases. in this study, we investigated the abundance and species of ticks associated with swine and their habitats to assess the risk of spread of tick-borne diseases in host species, such as wild boars. ticks were collected from 24 grazing or traditionally reared domestic pig farms and 8 habitats of wild boars in 8 provinces and 1 city in the republic of korea, by using the dragging and flagging methods. ticks we ...201728506041
two featured series of rrna-derived rna fragments (rrfs) constitute a novel class of small this study, we reported two featured series of rrna-derived rna fragments (rrfs) from the small rna sequencing (srna-seq) data of amblyomma testudinarium using the illunima platform. two series of rrfs (rrf5 and rrf3) were precisely aligned to the 5' and 3' ends of the 5.8s and 28s rrna gene. the rrf5 and rrf3 series were significantly more highly expressed than the rrfs located in the body of the rrna genes. these series contained perfectly aligned reads, the lengths of which varied progress ...201728441451
molecular and morphological identification of a human biting tick, amblyomma testudinarium (acari: ixodidae), in taiwan.genetic identity and morphological features of a human biting tick, amblyomma testudinarium, were determined for the first time in taiwan. morphological features of adult male and female ticks of am. testudinarium were observed and photographed by a stereo- microscope. the genetic identity was analyzed by comparing the sequences of mitochondrial 16s ribosomal dna gene obtained from 18 strains of ticks representing 10 species of amblyomma, and four outgroup species of dermacentor and rhipicephalu ...201728405839
molecular survey of babesia gibsoni using haemaphysalis longicornis collected from dogs and cats in japan.a nationwide survey of babesia gibsoni using haemaphysalis longicornis collected from dogs and cats in japan was conducted using molecular methods. a total of 1,341 h. longicornis, including 305 females, 14 males, 332 nymphs and 690 larvae (153 pools) from 44 prefectures, were examined by b. gibsoni-targeted pcr. partial sequence analysis revealed that 12 of 13 positive samples sequenced, including samples from tottori, hiroshima, yamaguchi, tokushima, ehime and oita prefectures (all in western ...201424920547
detection of rickettsia tamurae dna in ticks and wild boar (sus scrofa leucomystax) skins in shimane prefecture, japan.we used 24 wild boars trapped from december 2009 to january 2010 and a further 65 from july 2010 to august 2010 in misato town, shimane prefecture, japan. we collected blood, spleens, skins and ticks from the wild boars, which were examined for rickettsial infections using polymerase chain reaction (pcr) primers for the genes rickettsial 17-kda antigen and citrate synthase (glta). we amplified rickettsia tamurae at-1 dna from the tick amblyomma testudinarium and from wild boar skins where ticks ...201323075922
molecular detection of severe fever with thrombocytopenia syndrome and tick-borne encephalitis viruses in ixodid ticks collected from vegetation, republic of korea, 2014.ticks play an important role in transmission of arboviruses responsible for emerging infectious diseases, and have a significant impact on human, veterinary, and wildlife health. in the republic of korea (rok), little is known about information regarding the presence of tick-borne viruses and their vectors. a total of 21,158 ticks belonging to 3 genera and 6 species collected at 6 provinces and 4 metropolitan areas in the rok from march to october 2014 were assayed for selected tick-borne pathog ...201627211914
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