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histochemistry of gland cells of eurytrema pancreaticum cercariae.the cercariae of eurytrema pancreaticum (janson, 1889) possess four types of gland cells - proper cystogenic, penetration, ventral and dorsal gland cells. the secretion of ventral and dorsal gland cells is released into the tegument. the proper cystogenic gland cells are the largest and their contents serve for the formation of the cyst wall of metacercariae in the second intermediate host. the secretion of proper cystogenic gland cells contains besides neutral mucosubstances also acid mucosubst ...1979161544
[glycolysis in a pancreatic parasite of cattle, the trematode eurytrema pancreaticum].phosphoenol pyruvate carboxykinase activity in cytosol of e. pancreaticum is approximately 10 times higher than pyruvate kinase activity. malate dehydrogenase converts oxaloacetate to malate 27 times more actively than malate to oxaloacetate. the main end product of the glycolysis is presented by succinate. probably, the most important glycolytic pathway in the trematode investigated is the fixation of co2 to phosphoenol pyruvate to produce oxaloacetate.1978735591
[developmental periods and adaptation of parthenites of eurytrema pancreaticum (dicrocoeliidae, trematoda) to environmental conditions].data are given on the developmental periods of parthenites of e. pancreaticum (janson, 1889) from the far east of the ussr. the growth and formation of parthenites were found to take place from may to october and to coincide with the active period of the intermediate host's life cycle. with the decrease of autumn temperatures to 8 degrees the growth of parthenites slows down and ceases completely in october. the pause in the development of parthenites from october to april causes longer developm ...19761018937
mortalities and wasting in indonesian sheep associated with the trematode eurytrema pancreaticum. 19921455594
seasonal prevalence of some zoonotic trematode infections in cattle and pigs in the north-east montane zone in india.seasonal variations in the prevalence of fasciola gigantica and eurytrema pancreaticum in cattle, and opisthorchis noverca, artyfechinostomum malayanum, fasciolopsis buski and gastrodiscoides hominis in pigs, were studied post-necropsy over a 1 year period in a humid, subtropical north-east hilly region in india. the overall prevalence rate was 53.02% in cattle (n = 960) and 12.92% in pigs (n = 960). fasciola gigantica and e. pancreaticum occurred throughout the year with peaks during cold winte ...19921561763
[studies on molluscicidal effect of bromoacetamide against ganesella virgo].molluscicidal effect of bromoacetamide against the snails in laboratory and field trials were observed. grassland of guiliu river in keerqinyouyiquanqi district, neimengol autonomous region was selected for field trials. the results showed that bromoacetamide was fairly effective against ganesella virgo, the intermediate host of dicrocoelium chinensis and eurytrema pancreaticum. in field trials, 7 days after spraying at a dosage of 0.5g/m2, 74.7-84.7% of snails were killed. when a dosage of 1g/m ...19902364507
comparative histochemical studies of glycosidase activity in some helminths.comparative histochemical studies of glycosidase activity were carried out in clonorchis sinensis, eurytrema pancreaticum, fasciola hepatica, dipylidium caninum, hymenolepis nana, ascaris suum, toxocara canis, ancylostoma caninum, trichuris vulpis and dirofilaria immitis. the enzymes examined were: n-acetyl-beta-glucosaminidase (ec 3.2.1.30), beta-glucuronidase (ec 3.2.1.31) and beta-galactosidase (ec 3.2.1.23). there were variations in enzyme distribution and intensity among the species and als ...19863084622
[development of eurytrema pancreaticum (janson, 1889) (trematoda: dicrocoeliidae) in the maritime territory]. 19734786633
patterns of transmission of the trematode eurytrema pancreaticum in malaysia. 19665222978
completion of the life cycle of eurytrema pancreaticum (trematoda: dicrocoeliidae). 19655841331
human infection with the pancreas fluke, eurytrema pancreaticum.a 70-year-old japanese woman with gastric cancer in fukuoka prefecture, japan, was found at autopsy to have about 15 adult flukes, eurytrema pancreaticum, in the dilated pancreatic ducts. the number of eosinophils in the blood was within normal limits. the two worms which were least damaged when removed from the gland were used for stained specimens, and another two were macerated to collect the eggs. the parasites (10 x 5, 11 x 7 mm in size) were broad, flattened, oval to fusiform. the suckers ...19836625056
[effect of anthelmintic preparations on the carbohydrate metabolism of the trematode eurytrema pancreaticum]. 19846717390
[the adenosinetriphosphatases of trematodes parasitizing cattle and the effect on them of anthelmintic preparations].activities and properties of adenosine triphosphatases in mitochondrial and microsomal fractions of trematodes eurytrema pancreaticum and calicophoron ijimai were studied. the highest atpase activity was observed in mitochondrial fractions. the effect of anthelmintic preparations on activity of enzyme was investigated. trichlorophene, bitionol, oxinide, g-937 and g-1028 turned out to be the most effective preparations.19938265211
[isocitrate dehydrogenases of trematodes parasitizing cattle and the feasibility of inhibiting them using anthelmintic preparations].activities and properties of nadf-dependent isocitrate dehydrogenases in cytosol and mitochondrial fractions from trematodes eurytrema pancreaticum and calicophoron ijimai were examined. cytosol and mitochondrial enzymes were activated by ions mn2+ and mg2+ and inhibited by ions of heavy metals and p-chloromercuribenzoate. the effect of anthelmintic preparations on activity of enzymes was investigated.19989859128
production and characterization of a monoclonal antibody against recombinant fatty acid binding protein of fasciola gigantica.in fasciola parasites fatty acid binding proteins (fabps) are the carrier proteins that help in the uptake of fatty acids from the hosts' fluids. attempts have been made to utilize both native and recombinant fabp (rfabp) for immunodiagnosis and vaccine development for fasciolosis. in this study, we have produced a number of monoclonal antibodies (moabs) against rfabp of fasciola gigantica. these moabs were initially screened against rfabp by elisa and then tested for their specificities by immu ...200211900926
production and characterization of a monoclonal antibody against 28.5 kda tegument antigen of fasciola gigantica.a monoclonal antibody (moab) against the 28.5 kda tegumental antigen of fasciola gigantica was produced by the hybridoma technique using spleen cells from balb/c mice immunized with the tegumental extract from adult f. gigantica. this moab was found to be of the isotype igg(1), kappa-light chain, and shown by immunoblotting to specifically react with the 28.5 kda antigen present in the tegument, excretion-secretion material of the adult, whole-body extracts of newly excysted juveniles, 5-week-ol ...200212387905
[an epidemiological study on zoonoses in korea]helminthic zoonoses are of significant importance to the public health and to the socioeconomic consequences especially in lost-meat, animal products and animal labor. for past several decades in korea, it has been recognized that endoparasitic infections among domestic animals are extremely common and many of the helminth parasites of man have been acquired from, or are shared with domestic animals. however, there was no survey of a nationwide scale on the prevalence of animal helminthiasis; an ...198112902720
[study on the eurytrema pancreaticum: ii. life cycle]the life cycle of eurytrema pancreaticum has been studied locally at chejudo (quelpart island) in korea, and found a land snail, acusta despecta gray served as the first intermediate host of the pancreatic fluke. the second intermediate host of the fluke also has been surveyed there, the tettigoniid grasshoppers, conocephalus maculatus le guillon and c. gladiatius redtenbacher. the land snail, acusta despecta gray, is acquired infection with the eggs of the fluke in the autum, then the miracidia ...196912913532
[studies on phosphatase activity in some parasitic helminths]in order to obtain some informations on the nature and relative activity of the phosphatases present in various helminths, biochemical studies have been made in thirteen kinds of worm parasites including the adults and larvae (fasciola hepatica, eurytrema pancreaticum, paramphistomum sp., taenia solium, taenia pisiformis, dipylidium caninum, diphyllobothrium mansoni, cysticercus cellulosae, cysticercus fasciolaris and sparganum). a comparison based on the analysis of ph-activity curves was made ...196712913552
studies on transaminase reactions in some parasitic helminths.by an application of sigma-frankel methods, two transaminase systems, glutamic-pyruvic transaminase and glutamic-oxaloacetic transaminase, were found to operate at a mesurable rate in 2 species of nematodes(ascaris lumbricoides and ascaridia galli), 5 species of trematodes (clonorchis sinensis, fasciola hepatica, eurytrema pancreaticum, paramphistomum cervi and paragonimus westermani) and 5 kinds of cestodes (diphyllobothrium mansoni, dipylidium caninum, taenia pisiformis, cysticercus cellulosae ...196612913567
studies on the life history of eurytrema pancreaticum janson, 1889. 195014804225
infection status with helminthes in feral cats purchased from a market in busan, republic of korea.the present study was performed to investigate the infection status with helminth in a group of feral cats in korea. more than 29 helminth species including adults or eggs were detected in visceral and fecal samples of the examined cats. among these were a host of nematodes, including toxocarids, ancylostoma sp. and the larva of anisakis simplex; trematodes, including clonorchis sinensis, paragonimus westermani, eurytrema pancreaticum, pharyngostomum cordatum, metagonimus spp., heterophyes nocen ...200516192750
comparison of 18s ribosomal rna gene sequences of eurytrema coelmaticum and eurytrema pancreaticum.the partial 18s rrna sequences of e. coelmaticum and e. pancreaticum were amplified using conserved primers and an evolutionary tree was constructed using neighbor-joining. the percent identity of eurytrema species with other dicrocoeliidae varied from 97.5 to 98.2, while the percent identity between the two eurytrema species was up to 99.3. the tree showed that e. coelmaticum and e. pancreaticum were not situated in the same position, and they formed one cluster with l. collurioni. these result ...200717004097
[historical review on the development of medical parasitology in china during the years of 1871-2006].the present review deals with the representative research papers on human parasites and parasitic diseases in china over the past hundred years (1871-2006). as the views focused on the development of the medical parasitology, the historical background and progressive characters in the period of fermentation, origination, and expansion have been discussed. the check list of the first cases of human parasitic diseases reported in china during 1871-2006 contained 128 species of parasitic pathogens, ...200718038794
characterization and localization of saposin-like protein-2 (sap-2) in fasciola gigantica.fasciola gigantica saposin-like protein-2 (fgsap-2) belongs to a family of lipid-interacting proteins that are involved in the cytolysis of target cells. in this study, we have cloned and expressed fgsap-2 and produced the antibody against this recombinant protein. rabbit antiserum against rfgsap-2 reacted with a similar native protein in the whole body extracts of the 4-week-old juvenile and adult stage, as well as a protein in their excretion-secretion, but not in the tegument. in situ hybridi ...201021188603
production and characterization of a monoclonal antibody against recombinant cathepsin l1 of fasciola gigantica.monoclonal antibodies (moabs) against a recombinant cathepsin l1 of fasciola gigantica (rfgcatl1) were produced in vitro by fusion of balb/c mice spleen cells immunized with rfgcatl1 and mouse myeloma cells. reactivity and specificity of these moabs were evaluated by indirect elisa and immunoblotting techniques. seven moab clones were selected from the stable hybridoma clones, namely 1e10, 1f5, 3d11, 4b10, 4d3, 4e3 and 5e7. clones 1e10, 1f5 and 3d11 were igm, whereas clones 4b10, 4d3, 4e3 and 5e ...201424736227
trematodes recovered in the small intestine of stray cats in the republic of korea.in 2005, we reported the infection status of 438 stray cats with various species of intestinal helminths, including nematodes (4 species), trematodes (23 species), and cestodes (5 species) in the republic of korea. however, morphologic details of each helminth species have not been provided. in the present study, we intended to describe morphologic details of 13 trematode species which were either new fauna of cats (10 species) or new fauna of not only cats but also all animal hosts (3 species). ...201323467726
sequencing and characterization of the complete mitochondrial genome from the pancreatic fluke eurytrema pancreaticum (trematoda: dicrocoeliidae).the trematode eurytrema pancreaticum is a parasite of ruminant pancreatic and bile ducts, and also occasionally infects humans, causing eurytremiasis. in spite of it being a common fluke of cattle and sheep in endemic regions, little is known about the genomic resources of the parasite. we sequenced the complete mitochondrial (mt) genome of e. pancreaticum. it is 15,031 bp in size, and encodes 36 genes: 12 protein-coding genes, two ribosomal rna genes, and 22 transfer rna genes. the e. pancreati ...201626434796
update on trematode infections in sheep.trematode parasites live in the liver, fore stomachs or blood vessels of a wide range of animals and humans. most of them have a special economic and veterinary significance. liver fluke disease of sheep and other animal species is caused by the common liver fluke fasciola hepatica. hepatic fasciolosis occurs throughout the world, where climatic conditions are suitable for the survival of aquatic intermediate host snails. also of importance for ruminants, in some parts of the world, are fasciola ...201222521973
[influence of anthelmintics on enzyme systems of parasitic flatworms].literary and original data on the influence of some anthelmintic preparations on the survival in vitro and activity of twenty enzymes of gluconeogenesis, carbohydrate and energy metabolism in the trematode species eurytrema pancreaticum and calicophoron ijimai parasitizing cattle in the russian far east are summarized.200717578244
identification and characterization of micrornas in the pancreatic fluke eurytrema pancreaticum.eurytrema pancreaticum is one of the most common flukes, which mainly infects ruminants globally and infects human beings accidentally; causing eurytremiasis that has high veterinary and economic importance. micrornas (mirnas) are small non-coding rnas and are now considered as a key mechanism of gene regulation at the post-transcription level.201323351883
production and characterization of a monoclonal antibody against recombinant saposin-like protein 2 of fasciola gigantica.a monoclonal antibody (moab) against recombinant fasciola gigantica saposin-like protein 2 (rfgsap-2) was produced by hybridoma technique using spleen cells from balb/c mice immunized with rfgsap-2. this moab is an igg1, κ light chain isotype. by immunoblotting and indirect elisa, the moab reacted specifically with rfgsap-2, the natural fgsap-2 at 10kda in whole body (wb) and excretory-secretory (es) fractions of f. gigantica. it did not cross react with antigens in wb fractions from other paras ...201323164839
characterization of the complete nuclear ribosomal dna sequences of eurytrema pancreaticum.eurytrema pancreaticum is one of the most common trematodes of cattle and sheep, and also infects humans occasionally, causing great economic losses and medical costs. in this study, the sequences of the complete nuclear ribosomal dna (rdna) repeat units of five e. pancreaticum individuals were determined for the first time. they were 8306-8310 bp in length, including the small subunit (18s) rdna, internal transcribed spacer 1 (its1), 5.8s rdna, internal transcribed spacer 2 (its2), large subuni ...201728651672
helminth infections of sheep in north sumatra, indonesia.gastrointestinal tracts of 73 indigenous sheep were obtained from the medan abattoir in north sumatra, indonesia, and examined for the presence of helminths. a total of 13 species of helminths, eight nematodes, one cestode and four trematodes were encountered. all sheep were infected by more than one species of nematodes. trichostrongylus colubriformis, t. axei and haemonchus contortus were the most common species. the intensity of the nematode infections was very high: the average burden was ov ...19968720574
preliminary studies of lipids of the trematodes eurytrema pancreaticum, calicophoron erschowi and the turbellarian penecurva sibirica.the amount of total lipids, phospholipids, neutral lipids and fatty acids was determined in cattle parasites, namely the trematodes eurytrema pancreaticum, calicophoron erschowi, and in the free-living turbellarian penecurva sibirica. neutral lipids of these flatworms contained sterols, sterol esters, triacylglycerols and free fatty acids. p. sibirica also contained diacylglycerols. parasitic and free-living flatworms differed in phospholipid composition: the turbellarian did not contain phospha ...19827099203
morphology, histochemistry and ultrahistochemistry of special verrucose formations in daughter sporocyst of eurytrema pancreaticum.verrucose formations were found on the surface of fully developed sporocysts of e. pancreaticum janson, 1889 at the site where the attenuated proboscis-like anterior portion widens into the posterior portion. under these verrucose formations is always a group of gland cells. their narrowed processes pass at a common site through the muscle layer and above this layer again slightly widen and project above the neighbouring tegument. the tegument of the verrucose formation differs from the neighbou ...1978640522
monoclonal antibody against recombinant fasciola gigantica cathepsin l1h could detect juvenile and adult cathepsin ls of fasciola gigantica.cathepsin ls (catls), the major cysteine protease secreted by fasciola spp., are important for parasite digestion and tissue invasion. fasciola gigantica cathepsin l1h (fgcatl1h) is the isotype expressed in the early stages for migration and invasion. in the present study, a monoclonal antibody (moab) against recombinant f. gigantica cathepsin l1h (rfgcatl1h) was produced by hybridoma technique using spleen cells from balb/c mice immunized with recombinant profgcatl1h (rprofgcatl1h). this moab i ...201525324133
protection against fasciola gigantica infection in mice by vaccination with recombinant juvenile-specific cathepsin l.fasciola gigantica cathepsin l1h (fgcatl1h) is one of the major cathepsin l released by juveniles of f. gigantica to aid in the invasion of host's tissues. due to its high sequence similarity with other cathepsin l (catl) isoforms of late stage f. gigantica, it was considered to be a good vaccine candidate that can block all catl-mediated protease activities and affect juveniles as well as adult parasites. in this study, recombinant profgcatl1h protein expressed in yeast, pichia pastoris, system ...201525701317
morphological and molecular characterization of eurytrema cladorchis parasitizing cattle (bos indicus) in bangladesh.there is always controversy regarding identification of different species in the genus eurytrema. identification has been based mainly on morphology, which can be misleading and subject to differing interpretation among the scientists. therefore, the aim of this study was to identify eurytrema flukes both by morphology and molecular properties on the basis of 18-subunit ribosomal rna (18s rrna) gene as well as internal transcribed spacer 2 (its2) to clarify their phylogenetic status. among six d ...201525762187
de novo assembly and characterization of the transcriptome of the pancreatic fluke eurytrema pancreaticum (trematoda: dicrocoeliidae) using illumina paired-end sequencing.eurytrema pancreaticum is one of the most common trematodes living in the pancreatic and bile ducts of ruminants and also occasionally infects humans, causing eurytremiasis. in spite of its economic and medical importance, very little is known about the genomic resources of this parasite. herein, we performed de novo sequencing, assembly and characterization of the transcriptome of adult e. pancreaticum. approximately 36.4 million high-quality clean reads were obtained, and the length of the tra ...201626494161
antigenic profile, isolation and characterization of whole body extract of paramphistomum gracile.an antigenic component of adult paramphistomum gracile was characterized by means of indirect enzyme-linked immunosorbent assay (indirect elisa), sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page) and immunoblotting using sera from cattle naturally infected with p. gracile, eurytrema pancreaticum, fasciola gigantica, moniezia benedeni, strongylids, trichuris sp. and strongyloides sp. the whole body (wb) extracts of p. gracile were fractionated by gel filtration chromatography ...201627135198
cytosolic superoxide dismutase can provide protection against fasciola gigantica.superoxide dismutases (sod), antioxidant metallo-enzymes, are a part of the first line of defense in the trematode parasites which act as the chief scavengers for reactive oxygen species (ros). a recombinant fasciola gigantica cytosolic sod (fgsod) was expressed in escherichia coli bl21 (de3) and used for immunizing rabbits to obtain polyclonal antibodies (anti-rfgsod). this rabbit anti-rfgsod reacted with the native fgsod at a molecular weight of 17.5kda. the fgsod protein was expressed at high ...201627338185
production and characterization of a monoclonal antibody specific to 16 kda antigen of paramphistomum gracile.a number of monoclonal antibodies (moabs) against the 16 kda antigen of paramphistomum gracile (16 kdaagpg) were produced in vitro by hybridoma technique. reactivity and specificity of these moabs were evaluated by elisa and immunoblotting assays. seven moab clones were selected from the stable hybridoma clones, namely 1d10, 2d7, 3b10, 3d9, 4f1, 4g4, and 5g12. it was found to be igm and kappa light chain isotypes. by immunoblotting and elisa, all moabs reacted with purified 16 kdaagpg at molecul ...201727770198
eurytrema pancreaticum: the in vitro effect of praziquantel and triclabendazole on the adult fluke.the efficacy and tolerance of the 80 microg/ml praziquantel (pzq) and 40 microg/ml triclabendazole (tcz) against adult stage eurytrema pancreaticum in vitro were investigated at 3, 12, and 15 h incubation. motility of the flukes and histopathological changes were studied. sudden paralysis and death were observed after exposed to pzq as early as 3h incubation. in contrast, the tcz treated flukes showed active mobility at all intervals. by light microscopic examination, severe damages in various o ...200516125702
metabolism of c(14)-glucose by eurytrema pancreaticum.1. the glucose uptake rate by eurytrema pancreaticum was a mean value of 16.44 +/- 2.42 micro moles/hr/g, and total co2 production rate by the fluke averaged 5.82 +/- 0.97 micro moles/hr/g. the relative specific activity of respiratory co2 showed a mean value of 5.75 +/- 0.84 per cent. the rate of co2 production derived from medium c(14)-glucose was a mean of 0.33 +/- 0.10 micor-mole/hr/g. therefore, the average value of 0.32 +/- 0.04 per cent of glucose utilized by the flukes from the medium c( ...196412913599
autoradiographic studies on some parasitic helminth.direct contact method of autoradiography was utilized in studying the distribution of exogenous c(14)-proline in clonorchis sinensis, eurytrema pancreaticum, hymenolepis diminuta and dipylidium caninum. the most distinct autoradiogram were obtained after 60 min of incubation, corresponding to the maximal absorption of c(14)-proline in these parasite. the radioactivitity of this labeled amino acid was chiefly concentrated in reproductive organs, especially egg-containing uterine tubules.196412913597
[metabolism of c(14)-lactate by fasciola hepatica and eurytrema pancreaticum]the adult trematode, fasciola hepatica and eurytrema pancreaticum, employed in this experiment were obtained from the cattle slaughtered at the local abbatoir. the worms were selected and washed several times in normal sterilized saline solution. each ten of intact f. hepatica and about thirty to fifty of e. pancreaticum were incubated in 50 cc volume of special incubation flasks with incubation medium consisting of 50 cc of krebs-ringer phosohate buffer (ph 7.4). the incubation medium was added ...196512913590
[metabolism of c(14)-acetate by some trematodes]the adult trematodes, fasciola hepatica, eurytrema pancreaticum and paramphistomum cervi, employed in this experiment were obtained from the cattle slaughtered at the local abbatoir. the worms selected and washed several times in normal sterilized saline solution. each about ten of intact f. hepatica, fourty of e. pancreaticum, and twenty of p. cervi were incubated in 50 cc volume of special incubation flasks with incubation medium consisting of 10 cc. of krebs-ringer phosphate buffer(ph 7.4) th ...196512913583
[studies on the mineral contents of some trematodes]the mineral contents of the adult clonorchis sinensis from rabbits and human were measured, and its qualitative and semi-quantitative analyses were studied with 3.4 meter ebert-grating spectrograph, and its quantitative analyses were studied with beckman du spectrophotometer. the adult clonorchis sinensis used in this study were divided into two groups, the first group was collected from the bile passage of the man (c. sinensis from man), the second group was collected from the bile passage of t ...196512913579
[studies on lactic dehydrogenase activity in parasitic helminths]a series of experiments was performed to determine the lactic dehydrogenase activity of various parasitic helminths. the enzyme activity was determined by the modified method of wroblewshi and ladue (1955) using tissue homogenate of 16 kinds of worm parasites. the worms were mostly collected alive from local abattoir and removed from the organ or tissues of the naturally infected animal host and some materials were also obtained from the human host. they were thoroughly washed and homogenized in ...196712913559
[forms of glycogen and its localization in the parthenitae and larval stages of eurytrema pancreaticum (trematoda: dicrocoeliidae)]. 19817290740
[oxidation of krebs cycle substrates by eurytrema pancreaticum mitochondria].from tissues of e. pancreaticum were isolated mitochondria capable to swell under the effect of some factors. the intensive oxidation of succinate, isocitrate, cisaconitate, oxalacetate and alpha-ketoglutarate by mitochondria and less intensive one of malate, fumarate, citrate and pyruvate were shown. nad caused the rise in oxidation intensity of isocitrate, cis-aconitate, oxalacetate, alpha-ketoglutarate, malate, fumarate and pyruvate while nadp--of isocitrate and citrate.1977909726
cubic crystal protein inclusions in the neodermis of the pancreatic fluke, eurytrema pancreaticum, and eurytrema coelomaticum.light microscopy of eurytrema pancreaticum and eurytrema coelomaticum collected from cattle in japan, china, thailand, and brazil showed many cubic crystal inclusions in the neodermis (tegument) of all flukes. the crystal inclusions were histochemically positive for protein. scanning electron microscopy showed many cubic protrusions containing cubic crystal protein inclusions on the surface of the neodermis. transmission electron microscopy showed that cubic crystal protein inclusions appeared i ...200717657508
[the carbohydrate metabolic end products of trematodes parasitic in cattle].the trematodes eurytrema pancreaticum and calicophoron ijimai during the incubation in vitro assimilated glucose from the incubation medium and utilized the endogenous glycogen. final products of the carbohydrate metabolism in the calicophorones were lactic, acatic, propionic, isobutyric and alpha-methylbutyric acids; in the eurytremes they were lactic, acetic, propionic, isobutyric, alpha-methylbutyric, valerianic and capronic acids. the effect of anthelminthic preparations on the carbohydrate ...200010750153
[malate dehydrogenase and lactate dehydrogenase in trematodes and turbellarians].studies have been made on the activity and properties of malate and lactate dehydrogenases from the cattle rumen trematodes eurytrema pancreaticum, calicophoron ijimai and the turbellarian phagocata sibirica which has a common free-living ancestor with the trematodes. all the species studied have a highly active malate dehydrogenase, its activity in the reaction of reducing oxaloacetate being 6-14 times higher than in the reaction of malate oxidation. the affinity of malate dehydrogenase to oxal ...19863962529
fasciola gigantica: production and characterization of a monoclonal antibody against recombinant cathepsin b3.a number of monoclonal antibodies (moabs) against a recombinant cathepsin b3 (rcatb3) of fasciola gigantica were produced in balb/c mice. reactivity and specificity of these moabs were assessed by indirect elisa and immunoblotting techniques. six stable clones, namely 1c4, 1e9, 2e5, 2f9, 5b4, 5d7 were obtained. all moabs reacted with rcatb3 at molecular weight (mw) 37 kda as well as the glycosylated peptide at 55-75 kda and with the native catb3 at mw 37 kda in wb extracts of metacercariae (met) ...201120736007
[autoradiographic study on eurytrema pancreaticum]direct contact method of autoradiography was applied in order to know in eurytrema pancreaticum which was incubated at various intervals such as 60, 120, 240, and 360 minutes in the vitro media added with radioactive succinic acid, c(14)-succinate. the distribution of the radioactive succinate was clarified by this technique and possible explanation was presented. the autoradiographic procedures were essentially the same as those reported previously by yoon et al. (1964). the most distinct autor ...196712913554
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