determination of fruit origin by using 26s rdna fingerprinting of yeast communities by pcr-dgge: preliminary application to physalis fruits from egypt.the determination of geographical origin is a demand of the traceability system of import-export food products. one hypothesis for tracing the source of a product is by global analysis of the microbial communities of the food and statistical linkage of this analysis to the geographical origin of the food. for this purpose, a molecular technique employing 26s rdna profiles generated by pcr-dgge was used to detect the variation in yeast community structures of three species of physalis fruit (phys ...200919784935
diversity of bacterial endophytes in roots of mexican husk tomato plants (physalis ixocarpa) and their detection in the rhizosphere.endophytic bacterial diversity was estimated in mexican husk tomato plant roots by amplified rdna restriction analysis and sequence homology comparison of the 16s rdna genes. sixteen operational taxonomic units from the 16s rdna root library were identified based on sequence analysis, including the classes gammaproteobacteria, betaproteobacteria, actinobacteria, and bacilli. the predominant genera were stenotrophomonas (21.9%), microbacterium (17.1%), burkholderia (14.3%), bacillus (14.3%), and ...201021157706
antioxidant potential of agrobacterium-transformed and non-transformed physalis ixocarpa plants grown in vitro and ex vitro.oxidative stress is involved in pathogenesis of a number of chronic diseases hence is an increasing interest in plant-derived natural antioxidants with respect to their potential health benefits. plants from the genus physalis are particularly rich in secondary metabolites and show significant antioxidant potential. recent development in transgenic research has opened new possibilities for enhanced production of secondary metabolites with plant cell and organ cultures. the hairy root-regenerated ...201223687216
antimicrobial potentials of different solvent extracted samples from physalis ixocarpa.the present study investigates the antimicrobial activities of different solvent extracted samples isolated from different parts of physalis ixocarpa through disc diffusion assay using three different concentrations. statistical analysis of the data revealed that different parts of the plant showed varying degree of inhibition against different bacteria at different concentrations. different solvent extracted samples from the calyx showed inhibitory activity against most of the bacteria under st ...201627087074
agrobacterium-mediated transformation of tomatillo (physalis ixocarpa) and tissue specific and developmental expression of the camv 35s promoter in transgenic tomatillo plants.a protocol for the agrobacterium-mediated transformation of tomatillo was developed. up to 40 transgenic plants could be obtained in experiments using 60 cotyledon expiants. the transformed nature of the regenerated plants was confirmed by npt ii and southern blot hybridization analysis. using the b-glucuronidase system the tissue specific and developmental patterns of expression of the cauliflower mosaic virus 35s promoter were determined in transgenic tomatillo plants. it was found that this p ...199224213286
phytogrowth-inhibitory lactones derivatives of glaucolide b.the sesquiterpene lactone glaucolide b (1), isolated from vernonia fruticulosa (asteraceae), was transformed into six lactones (2-7). the structures of the products were elucidated by spectroscopic analysis. a series of solutions of compounds 1-7, at 200 microm, were tested on the germination and on the root and shoot growth of the dicotyledons physalis ixocarpa and trifolium alexandrinum and of the monocotyledons lolium multiflorum and amaranthus hypochondriacus. lactone 5 exhibited clear selec ...200915666538
mexican propolis flavonoids affect photosynthesis and seedling a continuous effort to find new natural products with potential herbicide activity, flavonoids acacetin (1), chrysin (2) and 4',7-dimethylnarangenin (3) were isolated from a propolis sample collected in the rural area of mexico city and their effects on the photosynthesis light reactions and on the growth of lolium perenne, echinochloa crus-galli and physalis ixocarpa seedlings were investigated. acacetin (1) acted as an uncoupler by enhancing the electron transport under basal and phosphoryl ...201526318278
the sesquiterpenes β-caryophyllene and caryophyllene oxide isolated from senecio salignus act as phytogrowth and photosynthesis inhibitors.the n-hexane extract of s. salignus plants inhibited atp synthesis and two sesquiterpenes, the β-caryophyllene (1) and caryophyllene oxide (2) were isolated from this nonpolar fraction. compound 1 inhibited by 42% the root elongation of physalis ixocarpa seedlings at 50 µg/ml and by 53% at 150 µg/ml, whereas at 150 µg/ml this compound only inhibited root elongation of echinochloa crus-galli by 30%. on the other hand, compound 2 had no effect on either germination or root and stem growth of e. cr ...201222310166
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