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an epidemic of dengue fever in wewak.143 clinical cases of dengue fever were reported in wewak between april and august 1976. 15 patients demonstrated a diagnostic rise in antibody titre for group b arbovirus. tests for complement-fixing antibody to dengue viruses did not indicate what type of dengue virus was responsible for the epidemic. vector species aedes aegypti and aedes scutellaris were found before control measures were adopted but were not found four months later. the epidemiology of dengue fever is discussed and measure ...1978279165
a light and electron microscope study of rickettsia-like organisms in the ovaries of mosquitoes of the aedes scutellaris group.the ovaries from some members of the aedes scutellaris group were examined by light and electron microscopy to locate rickettsia-like organisms, and culex pipiens fatigans was used as a control. it was found that light microscopy was unsatisfactory for this purpose since none of the available staining techniques demonstrates the organisms clearly and diagnostically. with the electron microscope it was shown that the organisms were by no means as common in the mosquitoes of the a. scutellaris gro ...1978666398
cross-insemination frequencies between strains of aedes albopictus and members of the aedes scutellaris group. 19715118866
linkage relationships of eleven enzyme loci in the aedes scutellaris group.linkage relationships of 11 enzyme loci were determined in backcrosses between aedes polynesiensis and aedes kesseli. three linkage groups established were aat2-lap2-me-sex, cat-ao-pgm-idh2-est6, and gpi-odh-pgd. lap2 and cat have not been previously mapped in aedes. locus order and linkage groups were the same as those observed for seven loci mapped in aedes aegypti. the significance of the observed similarities in chromosome organization and differences in crossover values among closely relate ...19836670992
the ultrastructure and symbiotic relationships of wolbachia of mosquitoes of the aedes scutellaris group. 19807411685
occurrence of aedes (stegomyia) spp. mosquitoes (diptera: culicidae) in halmahela villages, indonesia.aedes (stegomyia) spp. mosquitoes breeding in containers in and around human houses were surveyed in halmahela villages, the north moluccas, indonesia. adults reared from larvae found in containers and those reared from eggs collected by ovitraps revealed the dominance of aedes scutellaris (walker) in residential areas. breeding of aedes aegypti (l.) and aedes albopictus (skuse) was confirmed in a few coastal villages with high human densities.19968906924
generation of a novel wolbachia infection in aedes albopictus (asian tiger mosquito) via embryonic microinjection.genetic strategies that reduce or block pathogen transmission by mosquitoes are being investigated as a means to augment current control measures. strategies of vector suppression and replacement are based upon intracellular wolbachia bacteria, which occur naturally in many insect populations. maternally inherited wolbachia have evolved diverse mechanisms to manipulate host insect reproduction and promote infection invasion. one mechanism is cytoplasmic incompatibility (ci) through which wolbach ...200515944085
discovery of a widespread infestation of aedes albopictus in the torres strait, australia.aedes albopictus is a container-breeding stegomyia mosquito that has dispersed widely from its origins in southeast asia. because ae. albopictus is a known dengue vector and a potential vector of a variety of arboviruses and it can tolerate cooler climates than aedes aegypti, australian quarantine and health authorities have strategies to detect and eliminate it from international ports. following the detection of 42 adult ae. albopictus in bg-sentinel traps set on yorke island in the torres str ...200617067032
interspecific hybridization yields strategy for south pacific filariasis vector elimination.lymphatic filariasis (lf) is a leading cause of disability in south pacific regions, where >96% of the 1.7 million population are at risk of lf infection. as part of current global campaign, mass drug administration (mda) has effectively reduced lymphatic filiariasis prevalence, but mosquito vector biology can complicate the mda strategy. in some regions, there is evidence that the goal of lf elimination cannot be attained via mda alone. obligate vector mosquitoes provide additional targets for ...200818235849
infection and dissemination of dengue virus type 2 in aedes aegypti, aedes albopictus, and aedes scutellaris from the torres strait, australia.to determine their relative roles in transmission of dengue virus (denv) in the torres strait region of northern australia, we examined infection and dissemination of a sympatric strain of dengue virus type 2 (denv-2) in aedes scutellaris, ae. albopictus, and ae. aegypti. in experiments using membrane feeders for virus exposure, infection rates were 83% and 43% for ae. scutellaris and ae. aegypti, respectively. salivary gland infection rates for both species were 43%. in experiments using pledge ...200718240514
universal primers for the amplification and sequence analysis of actin-1 from diverse mosquito species.we report the development of universal primers for the reverse-transcription polymerase chain reaction (rt-pcr) amplification and nucleotide sequence analysis of actin cdnas from taxonomically diverse mosquito species. primers specific to conserved regions of the invertebrate actin-1 gene were designed after actin cdna sequences of anopheles gambiae, bombyx mori, drosophila melanogaster, and caenorhabditis elegans. the efficacy of these primers was determined by rt-pcr with the use of total rna ...201020649132
heat sensitivity of wmel wolbachia during aedes aegypti development.the wmel strain of wolbachia bacteria is known to prevent dengue and zika virus transmission in the mosquito vector aedes aegypti. accordingly, the release of wmel-infected a. aegypti in endemic regions has been recommended by the world health organization as a potential strategy for controlling dengue and zika outbreaks. however, the utility of this approach could be limited if high temperatures in the aquatic habitats where a. aegypti develop are detrimental to wolbachia. we exposed wmel-infec ...201627459519
factors predicting severe dengue in patients with dengue fever.dengue virus (denv) affects over half the world's population in 112 countries, and dengue fever (df) is the second largest arthropod borne infectious global hazard after malaria with complications like dengue hemorrhagic fever (dhf) and dengue shock syndrome (dss), accounting for significant morbidity and mortality world-over. pakistan is significantly affected with denv infection and to-date no study identifying risk factors associated with development of severe complications of df has been don ...201323505602
dengue: a growing menace -- a snapshot of recent facts, figures & remedies.dengue is specially owing to inadequate water supply and poor solid waste management , which are favorable for multiplication of the main vectors including the aedes ageypti coupled with lack of proven anti viral therapy and no proven efficient vaccine .there are many cases of both dengue shock syndrome and dengue haemmorhagic fever making it a major public health burden sending ominous signal resulting both rising morbidity & mortality, deleterious effect on daly [disability adjusted life year] ...023847455
knowledge, attitude, and practices regarding vector-borne diseases in western jamaica.outbreaks of vector-borne diseases (vbds) such as dengue and malaria can overwhelm health systems in resource-poor countries. environmental management strategies that reduce or eliminate vector breeding sites combined with improved personal prevention strategies can help to significantly reduce transmission of these infections.027036722
aedes hensilli as a potential vector of chikungunya and zika viruses.an epidemic of zika virus (zikv) illness that occurred in july 2007 on yap island in the federated states of micronesia prompted entomological studies to identify both the primary vector(s) involved in transmission and the ecological parameters contributing to the outbreak. larval and pupal surveys were performed to identify the major containers serving as oviposition habitat for the likely vector(s). adult mosquitoes were also collected by backpack aspiration, light trap, and gravid traps at se ...201425299181
mosquito larvicidal activity of aloe vera (family: liliaceae) leaf extract and bacillus sphaericus, against chikungunya vector, aedes aegypti.the bio-efficacy of aloe vera leaf extract and bacterial insecticide, bacillus sphaericus larvicidal activity was assessed against the first to fourth instars larvae of aedes aegypti, under the laboratory conditions. the plant material was shade dried at room temperature and powdered coarsely. a. vera and b. sphaericus show varied degrees of larvicidal activity against various instars larvae of a. aegypti. the lc50 of a. vera against the first to fourth instars larvae were 162.74, 201.43, 253.30 ...201223961212
open field release of genetically engineered sterile male aedes aegypti in malaysia.dengue is the most important mosquito-borne viral disease. in the absence of specific drugs or vaccines, control focuses on suppressing the principal mosquito vector, aedes aegypti, yet current methods have not proven adequate to control the disease. new methods are therefore urgently needed, for example genetics-based sterile-male-release methods. however, this requires that lab-reared, modified mosquitoes be able to survive and disperse adequately in the field.201222970102
human to mosquito transmission of dengue viruses.the successful transmission of dengue virus from a human host to a mosquito vector requires a complex set of factors to align. it is becoming increasingly important to improve our understanding of the parameters that shape the human to mosquito component of the transmission cycle so that vaccines and therapeutic antivirals can be fully evaluated and epidemiological models refined. here we describe these factors, and discuss the biological and environmental impacts and demographic changes that ar ...201424987394
relations of wolbachia infection with phylogeography of philaenus spumarius (hemiptera: aphrophoridae) populations within and beyond the carpathian contact zone.wolbachia is the most widespread intracellular α-proteobacteria maternally inherited endosymbiont of insects and nematodes. these bacteria are associated with a number of different reproductive phenotypes of their hosts. relatively few studies have dealt with distribution of infections across populations and with the influence of these bacteria on host genetic diversification and speciation. the aims of this study are to determine the distribution and rate of infection and to characterize the wo ...201525681033
dna barcoding and wing morphometrics to distinguish three aedes vectors in thailand.aedes aegypti (diptera: culicidae) (l.), ae. albopictus (skuse), and ae. scutellaris (walker) are important mosquito vectors of dengue and chikungunya viruses. they are morphologically similar and sympatric in some parts of their distribution; therefore, there is a risk of incorrect morphological identification. any confusion could have a negative impact on epidemiological studies or control strategies. therefore, we explored two modern tools to supplement current morphological identification: d ...201626987285
variation in cold hardiness of nondiapausing eggs of nine aedes (stegomyia) species (diptera: culicidae) from eastern asia and pacific islands ranging from the tropics to the cool-temperate zone.variation in cold hardiness was studied in the laboratory for the nondiapausing eggs of nine aedes (stegomyia) species from eastern asia and pacific islands, ranging from the tropics to the cool-temperate zone. species included were aedes aegypti (l.), aedes albopictus (skuse), aedes flavopictus yamada, aedes galloisi yamada, aedes hensilli farner, aedes palauensis bohart, aedes paullusi stone and farner, aedes riversi bohart and ingram, and aedes scutellaris (walker). for species with distribut ...201121485356
rapid identification of aedes albopictus, aedes scutellaris, and aedes aegypti life stages using real-time polymerase chain reaction assays.in 2005, a widespread infestation of aedes albopictus was discovered in the torres strait, the region between northern australia and new guinea. to contain this species, an eradication program was implemented in 2006. however, the progress of this program is impeded by the difficulty of morphologically separating ae. albopictus larvae from the endemic species aedes scutellaris. in this study, three real-time taqman polymerase chain reaction assays that target the ribosomal internal transcribed s ...200819052295
insecticidal effect of aliphatic alcohols against aquatic stages of aedes mosquitoes.effectiveness of certain long carbon chain aliphatic alcohols as ovicides and larvicides was investigated under laboratory conditions against eggs and larvae of aedes aegypti and aedes scutellaris. out of the eight alcohols tested, three (1-decanol, 1-undecanol and 1-dodecanol) proved to be very effective. the hatching of mosquito eggs was inhibited at a dosage of 5 to 7 litres/hectare. first, third and fourth instar larvae were killed at a dosage of 3 to 7 litres/hectare. first instar larvae we ...19836683011
the virulent wolbachia strain wmelpop increases the frequency of apoptosis in the female germline cells of drosophila melanogaster.wolbachia are bacterial endosymbionts of many arthropod species in which they manipulate reproductive functions. the distribution of these bacteria in the drosophila ovarian cells at different stages of oogenesis has been amply described. the pathways along which wolbachia influences drosophila oogenesis have been, so far, little studied. it is known that wolbachia are abundant in the somatic stem cell niche of the drosophila germarium. a checkpoint, where programmed cell death, or apoptosis, ca ...201222375935
increased female mortality as a barrier to hybridization between members of the aedes scutellaris complex of mosquitoes.interspecific crosses between the mosquitoes aedes polynesiensis and aedes malayensis have shown a unidirectional pattern of compatibility. aedes polynesiensis females inseminated by ae. malayensis males fail to produce viable offspring while the reciprocal cross is viable. in both crosses, rates of insemination are comparable to control rates. the ae. polynesiensis females fail to lay eggs. one apparent reason for this is that the ae. polynesiensis females have a high rate of mortality after in ...20123504907
effects of wolbachia in the uzifly, exorista sorbillans, a parasitoid of the silkworm, bombyx mori.the uzifly, exorista sorbillans (diptera: tachinidae), a parasitoid of the silkworm, bombyx mori l. (lepidoptera: bombycidae), harbours wolbachia (rickettsia) endosymbionts. administration of 0.05 mg/ml oxytetracycline to the adult uziflies removed wolbachia endosymbionts and resulted in different reproductive disorders, such as i) reduction in fecundity of uninfected females, ii) cytoplasmic incompatibility in crosses between infected males and uninfected females, iii) sterility in the crosses ...200517119612
population differentiation and wolbachia phylogeny in mosquitoes of the aedes scutellaris group.mosquito species of the aedes (stegomyia) scutellaris (walker) group (diptera: culicidae) are distributed across many islands of the south pacific and include major regional vectors of filariasis, such as aedes polynesiensis (marks). analysis of populations of ae. polynesiensis at the extremes of its range, from fiji and from moorea, french polynesia, using the rdna its2 (internal transcribed spacer 2) region and six microsatellite markers showed considerable genetic differentiation between them ...200515752179
endosymbiotic relationship between a rickettsia-like microorganism and the male germ-cells of culex tigripes.the male mosquitoes of the species culex tigripes possess endosymbionts localized in the cytoplasm of the germ-cells. the somatic cells of the testis do not exhibit this particularity. the spermatogonia, spermatocytes and spermatids all possess a few symbionts. they are eliminated in the residual body at the end of spermiogenesis when the spermatozoon matures. they are absent from the seminal vesicles. a few spermatocytes and spermatids show a proliferation of the endosymbionts. this seems to be ...19938096432
non-mendelian inheritance of mosquito susceptibility to infection with brugia malayi and brugia pahangi.the mode of inheritance of susceptibility or refractoriness of insect vectors to medically important pathogens such as those causing malaria or filariasis is usually believed to follow normal mendelian laws and to involve a single pair of alleles. in this report, experiments are described that demonstrate another mode of inheritance of mosquito susceptibility to filarial parasites. crosses were made between susceptibile and refractory species of the aedes scutellaris complex, and the hybrid and ...19817466401
susceptibility of the autogenous group of the aedes scutellaris complex of mosquitoes to infection with brugia malayi and brugia pahangi.four species of mosquitoes which represent the tonga group of the aedes scutellaris complex (ae. cooki, ae. kesseli, ae. tongae tabu and an undescribed aedes sp. nuaofo'ou) were tested for susceptibility to infection with brugia malayi and b. pahangi. all tested strains were genetically fully (100%) susceptible to infection with both parasitic helminths. higher survival of females harboring low quantities of infective larvae (1-9 l3/male) indicates a weak adaptation of the host to the parasite. ...19817345683
studies on the inheritance of susceptibility to infection with brugia pahangi and wuchereria bancrofti in the aedes scutellaris group of mosquitoes. 19826751252
occurrence of rickettsia-like symbionts among species of the aedes scutellaris group (diptera:culicidae).rickettsia-like organisms were seen by electron microscopy in the oocytes and nurse cells of aedes hebrideus and a. scutellaris scutellaris and in the oocytes of a. pseudoscutellaris, a samoan stock of a. polynesiensis and in three fijian stocks of a. polynesiensis. none were found in the ovaries of a. alcasidi, a. s. katherinensis, a. cooki nor in a second stock of samoan a. polynesiensis. it is suggested that the distribution of rickettsia-like symbionts among species of the a. scutellaris gro ...19846433816
vertical transmission of dengue viruses by mosquitoes of the aedes scutellaris group.seventeen strains of mosquitoes belonging to 12 species in the aedes scutellaris subgroup were tested for an ability to transmit one or more dengue virus serotype(s) vertically. strains of virus employed for dengue types 1, 2, 3, and 4 were from fiji, bangkok, burma, and medan, respectively. after parental females were infected by intrathoracic inoculation, f1 larval and pupal progeny were tested for the presence of virus by inoculating aliquots of triturated suspensions into toxorhynchites ambo ...19873688318
the effect of brugia pahangi infection on survival of susceptible and refractory species of the aedes scutellaris complex.life table statistics were used to examine the survival functions of filarial susceptible and refractory species of the aedes scutellaris (walker) group of mosquitoes, following infection with high and moderate doses of brugia pahangi (buckley & edeson). survivorship curves and hazard function curves were generated, and the median survival times and the proportions of mosquitoes surviving beyond the extrinsic incubation period of the parasite were determined. in the susceptible populations of ae ...19872979549
the exsheathment and migration of brugia pahangi microfilariae in mosquitoes of the aedes scutellaris species complex.studies on the aedes scutellaris complex of mosquitoes showed that refractory and susceptible species can be distinguished by reference to the proportion of brugia pahangi microfilariae which reach the thorax within three hours of a feed. migration ceased approximately 1 1/2 hours after feeding in refractory species, in which only 50% of the ingested worms reached the thorax, in susceptible species over 95% of the ingested microfilariae reached the thorax, and migration lasted for three hours af ...1978736663
ethological divergence in allopatry and asymmetrical isolation in the south pacific aedes scutellaris subgroup.ethological isolation among eight species of mosquitoes in the aedes scutellaris subgroup of the south pacific was investigated by offering females a simultaneous choice between males of their own species and males of another species. the degree of ethological isolation between these largely allopatric, island-dwelling mosquitoes was associated with time since the species had become geographically isolated by the fragmentation of the outer melanesian arc 2-10 mya. the degree of ethological isola ...198528561501
variation in mitochondrial dna of aedes species (diptera: culicidae).relationships among seven species of the aedes scutellaris subgroup and four of the aedes albopictus subgroup were determined using restriction fragment analysis of mtdna. considerable polymorphism was found among species, with an average proportion of 0.338 restriction fragments being shared among them. fitch-margoliash, wagner parsimony and upgma analyses revealed that the phylogeny based on mtdna was only partially congruent with that based on allozymes and morphology. all three analyses, how ...199128564078
inheritance of autogeny in the aedes scutellaris subgroup of mosquitoes. 1976978692
genetics of hematophagy and autogeny in the aedes scutellaris complex (diptera: culicidae). 1978731668
white-eye, a new mutant in aedes scutellaris katherinensis.white-eye (w), a new mutant in aedes scutellaris katherinensis woodhill, of the aedes (stegomyia) scutellaris subgroup is described. it is controlled by a single autosomal recessive gene, and is detectable at all developmental stages. penetrance is complete and its viability and expressivity are good. it is an excellent genetic marker.20066886378
effects of a juvenile hormone analogue on the morphology and biology of aedes scutellaris malayensis colless (diptera: culicidae).effects of 2-methyl-9(4-isopropylphenyl)-2,6-dimethyl nonane, or mv-678, a juvenile hormone analogue, on aedes scutellaris malayensis colless were investigated under laboratory conditions (29 degrees +/- 2 degrees c and 86 +/- 3% rh). the mv-678 was tested against the first, second, third and fourth instar larvae. the concentrations used were 0.0032, 0.016, 0.08, 0.4 and 2.0 mg/l. the morphogenetic aberrations were determined and divided into 8 groups, among which they included (1) death larvae, ...19854023815
intraspecific and interspecific variation in the sequence and abundance of highly repeated dna among mosquitoes of the aedes albopictus subgroup.interspecific variation in abundance of highly repeated dna sequences has been examined in three species of the aedes scutellaris and three species of the a. albopictus subgroup of the a. scutellaris group. sequences from a population of a. albopictus were hybridised with whole genomic content from other species and strains. copy number estimates were determined by dot-blot hybridisation. variation in sequence abundance between strains of a. albopictus was as great as between it and the other si ...19873597124
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