| rapid identification of nine species of diphyllobothriidean tapeworms by pyrosequencing. | the identification of diphyllobothriidean tapeworms (cestoda: diphyllobothriidea) that infect humans and intermediate/paratenic hosts is extremely difficult due to their morphological similarities, particularly in the case of diphyllobothrium and spirometra species. a pyrosequencing method for the molecular identification of pathogenic agents has recently been developed, but as of yet there have been no reports of pyrosequencing approaches that are able to discriminate among diphyllobothriidean ... | 2016 | 27853295 |
| genetic identification of spirometra decipiens plerocercoids in terrestrial snakes from korea and china. | human sparganosis is a zoonotic disease caused by infection with larval forms (procercoid/plerocercoid) of spirometra spp. the purpose of this study was to identify spirometra spp. of infected snakes using a multiplex pcr assay and phylogenetic analysis of mitochondrial dna sequence data from the spargana of terrestrial snakes obtained from korea and china. a total of 283 snakes were obtained that included 4 species of colubridae comprising rhabdophis tigrinus tigrinus (n=150), dinodon rufozonat ... | 2016 | 27180576 |
| mitochondrial genome sequences of spirometra erinaceieuropaei and s. decipiens (cestoidea: diphyllobothriidae). | the present study was performed to compare the mitochondrial genomes between 2 spirometra tapeworms, spirometra erinaceieuropaei and spirometra decipiens (cestoidea: diphyllobothriidae), which larval stages are important etiological agents of sparganosis in humans. for each species, the full mitochondrial genome was amplified in 8 overlapping fragments using total genomic dna purified from a single worm as the template. the mitochondrial genomes were 13,643 bp (s. erinaceieuropaei) and 13,641 bp ... | 2015 | 26323844 |
| human infections with spirometra decipiens plerocercoids identified by morphologic and genetic analyses in korea. | tapeworms of the genus spirometra are pseudophyllidean cestodes endemic in korea. at present, it is unclear which spirometra species are responsible for causing human infections, and little information is available on the epidemiological profiles of spirometra species infecting humans in korea. between 1979 and 2009, a total of 50 spargana from human patients and 2 adult specimens obtained from experimentally infected carnivorous animals were analyzed according to genetic and taxonomic criteria ... | 2015 | 26174823 |
| molecular detection of spirometra decipiens in the united states. | the genus spirometra belongs to the family diphyllobothriidae and order pseudophyllidea, and includes intestinal parasites of cats and dogs. in this study, a plerocercoid labeled as spirometra mansonoides from the usa was examined for species identification and phylogenetic analysis using 2 complete mitochondrial genes, cytochrome c oxidase i (cox1) and nadh dehydrogenase subunit 3 (nad3). the cox1 sequences (1,566 bp) of the plerocercoid specimen (usa) showed 99.2% similarity to the reference s ... | 2016 | 27658603 |