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identification, characterization, and detection of black raspberry necrosis virus.abstract a serious disease was observed in black raspberry (rubus occidentalis) in oregon in the last decade. plants showing mosaic symptoms declined rapidly and, in many cases, died after several years. double-stranded rna extraction from symptomatic black raspberry revealed the presence of two high molecular weight bands which were cloned and sequenced. sequence analysis disclosed the presence of a novel virus that was tentatively named black raspberry decline-associated virus (brdav). the com ...200718942935
the complete sequence of a uk strain of black raspberry necrosis virus.the complete nucleotide sequence of a uk strain of the sadwavirus black raspberry necrosis virus (brnv) was obtained by amplification and sequencing of virus rna from infected plants grown in a raspberry plantation in aylth, scotland. the rna1 was 7,572 nucleotides (nt) in size and rna2 was 6,350 nt in size, each excluding the 3' poly-a tail. the rna1- and rna2-encoded polyproteins are predicted to be processed into (rna1) a protease cofactor, an rna helicase, the vpg, a 3c-like protease, an rna ...201020878193
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