| A new begomovirus associated with alpha- and betasatellite molecules isolated from Vernonia cinerea in China. | A begomovirus disease complex associated with Vernonia cinerea showing yellow vein symptoms was studied. The full-length genomic DNA was comprised of 2739 nucleotides (nt) and contained the typical genome structure of begomoviruses. Comparison analysis showed that it shared the highest (78.9%) nucleotide sequence identity with recently characterized Vernonia yellow vein virus (VeYVV) from India. For associated satellites, betasatellite showed the highest nucleotide sequence identity (52.1%) with ... | 2011 | 22006044 |
| a simple and efficient method for agroinfection of vernonia cinerea with infectious clones of vernonia yellow vein virus. | vernonia yellow vein virus (veyvv) is a distinct monopartite begomovirus associated with a satellite dna β. after constructing dimers of both dna a and dna β in binary vectors, a number of infection methods were attempted. however, only a modified stem-prick method produced up to 83% infection in the natural host vernonia cinerea, thus, fulfilling the koch's postulate. the presence of the viral dna in the agroinfected plants was confirmed by rolling circle amplification (rca), followed by southe ... | 2011 | 21850413 |
| molecular characterization of a distinct begomovirus species from vernonia cinerea and its associated dna-beta using the bacteriophage phi 29 dna polymerase. | vernonia cinerea plants with yellow vein symptoms were collected around crop fields in madurai. a portion (550 bp) of the av1 gene amplified using degenerate primers from the total dna purified from diseased leaf sample was cloned and sequenced. specific primers derived from the above sequence were used to amplify 2,745 nucleotides with the typical genome organization of begomoviral dna a (embl accession no. am182232). sequence comparison with other begomoviruses revealed the greatest identity ( ... | 2010 | 20401528 |