| electron microscopic cytochemistry of polysaccharides in bacteria xanthomonas fuscans, the cause of the bean fuscous blight. | high resolution cytochemical staining of polysaccharides in the cells of the bean fuscous blight bacteria xanthomonas fuscans (burkholder) bur. revealed extensive deposits of a periodic acid-oxidizable compound in the cytoplasm, which accumulated at the onset of polysaccharide excretion and occupied a substantial part of the cell volume. in the course of subsequent culture growth the intracellular polysaccharides gradually disappeared and polysaccharide microfibrils were visualized in the medium ... | 1979 | 473968 |
| production and characteristics of the exocellular polysaccharide in mutant strains of xanthomonas fuscans. | production of the exocellular polysaccharide of the phytopathogenic bacterium xanthomonas fuscans was investigated with respect to its possible use in utilization of industrial wastes containing lactose. six stable lac+ mutants were obtained after the treatment with n-methyl-n-nitroso-n'-nitroguanidine. the mutants were compared with the parent strain. morphological and cultivation characteristics, as well as production of the exocellular polysaccharide were compared. the production was found to ... | 1977 | 838408 |
| the type iii secretion system of xanthomonas fuscans subsp. fuscans is involved in the phyllosphere colonization process and in transmission to seeds of susceptible beans. | understanding the survival, multiplication, and transmission to seeds of plant pathogenic bacteria is central to study their pathogenesis. we hypothesized that the type iii secretion system (t3ss), encoded by hrp genes, could have a role in host colonization by plant pathogenic bacteria. the seed-borne pathogen xanthomonas fuscans subsp. fuscans causes common bacterial blight of bean (phaseolus vulgaris). directed mutagenesis in strain cfbp4834-r of x. fuscans subsp. fuscans and bacterial popula ... | 2008 | 18326683 |
| assessment of the genetic diversity of xanthomonas axonopodis pv. phaseoli and xanthomonas fuscans subsp. fuscans as a basis to identify putative pathogenicity genes and a type iii secretion system of the spi-1 family by multiple suppression subtractive hybridizations. | fluorescent amplified fragment length polymorphism revealed that strains of xanthomonas axonopodis pv. phaseoli and xanthomonas fuscans subsp. fuscans are genetically distinct and can be grouped into four genetic lineages. four suppression subtractive hybridizations were then performed to isolate dna fragments present in these bean pathogens and absent from closely related xanthomonads. virulence gene candidates were identified such as homologs of hemagglutinins, tonb-dependent receptors, zinc-d ... | 2008 | 18359831 |
| phylogenetic structure of xanthomonas determined by comparison of gyrb sequences. | previously, we have produced a phylogeny of species type strains from the plant-pathogenic genus xanthomonas based on gyrb sequences. to evaluate this locus further for species and infraspecies identification, we sequenced an additional 203 strains comprising all the pathovar reference strains (which have defined plant hosts), 67 poorly characterized pathovars, currently classified as xanthomonas campestris, and 59 unidentified xanthomonads. the well-characterized pathovars grouped either in cla ... | 2009 | 19196764 |
| identification of xanthomonas fragariae, xanthomonas axonopodis pv. phaseoli, and xanthomonas fuscans subsp. fuscans with novel markers and using a dot blot platform coupled with automatic data analysis. | phytosanitary regulations and the provision of plant health certificates still rely mainly on long and laborious culture-based methods of diagnosis, which are frequently inconclusive. dna-based methods of detection can circumvent many of the limitations of currently used screening methods, allowing a fast and accurate monitoring of samples. the genus xanthomonas includes 13 phytopathogenic quarantine organisms for which improved methods of diagnosis are needed. in this work, we propose 21 new xa ... | 2011 | 21705524 |
| high throughput quantitative phenotyping of plant resistance using chlorophyll fluorescence image analysis. | in order to select for quantitative plant resistance to pathogens, high throughput approaches that can precisely quantify disease severity are needed. automation and use of calibrated image analysis should provide more accurate, objective and faster analyses than visual assessments. in contrast to conventional visible imaging, chlorophyll fluorescence imaging is not sensitive to environmental light variations and provides single-channel images prone to a segmentation analysis by simple threshold ... | 2013 | 23758798 |
| draft genome sequence of the flagellated xanthomonas fuscans subsp. fuscans strain cfbp 4884. | we report the draft genome sequence of the flagellated strain cfbp 4884 of xanthomonas fuscans subsp. fuscans, which was isolated in an outbreak of common bacterial blight of beans along with non-flagellated strains. comparative genomics will allow one to decipher the genomic diversity of strains cohabiting in epidemics. | 2014 | 25291773 |
| enhancement of a novel extracellular uricase production by media optimization and partial purification by aqueous three-phase system. | uricase (urate: oxygen oxidoreductase, ec 1.7.3.3), an enzyme belonging to the class of oxidoreductases, catalyzes the enzymatic oxidation of uric acid to allantoin and finds a wide variety of application as therapeutic and clinical reagent. in this study, uricase production ability of the bacterial strains isolated from deep litter poultry soil is investigated. the strain with maximum extracellular uricase production capability was identified as xanthomonas fuscans subsp. aurantifolii based on ... | 2015 | 25674929 |
| genome sequencing reveals a new lineage associated with lablab bean and genetic exchange between xanthomonas axonopodis pv. phaseoli and xanthomonas fuscans subsp. fuscans. | common bacterial blight is a devastating seed-borne disease of common beans that also occurs on other legume species including lablab and lima beans. we sequenced and analyzed the genomes of 26 strains of xanthomonas axonopodis pv. phaseoli and x. fuscans subsp. fuscans, the causative agents of this disease, collected over four decades and six continents. this revealed considerable genetic variation within both taxa, encompassing both single-nucleotide variants and differences in gene content, t ... | 2015 | 26500625 |
| molecular characterization of xopag effector avrgf2 from xanthomonas fuscans ssp. aurantifolii in grapefruit. | xanthomonas fuscans ssp. aurantifolii group c strains exhibit host specificity on different citrus species. the strains possess a type iii effector, avrgf2, belonging to the xopag effector gene family, which restricts host range on citrus. we dissected the modular nature and mode of action of avrgf2 in grapefruit resistance. xopag effectors possess characteristic features, such as a chloroplast localization signal, a cyclophilin-binding domain characteristic amino acid sequence motif (gpll) and ... | 2017 | 27030294 |
| genomes-based phylogeny of the genus xanthomonas. | the genus xanthomonas comprises several plant pathogenic bacteria affecting a wide range of hosts. despite the economic, industrial and biological importance of xanthomonas, the classification and phylogenetic relationships within the genus are still under active debate. some of the relationships between pathovars and species have not been thoroughly clarified, with old pathovars becoming new species. a change in the genus name has been recently suggested for xanthomonas albilineans, an early br ... | 2012 | 22443110 |
| genome sequence of xanthomonas fuscans subsp. fuscans strain 4834-r reveals that flagellar motility is not a general feature of xanthomonads. | xanthomonads are plant-associated bacteria responsible for diseases on economically important crops. xanthomonas fuscans subsp. fuscans (xff) is one of the causal agents of common bacterial blight of bean. in this study, the complete genome sequence of strain xff 4834-r was determined and compared to other xanthomonas genome sequences. | 2013 | 24195767 |
| rapid and sensitive detection of citrus bacterial canker by loop-mediated isothermal amplification combined with simple visual evaluation methods. | citrus bacterial canker (cbc) is a major, highly contagious disease of citrus plants present in many countries in asia, africa and america, but not in the mediterranean area. there are three types of citrus bacterial canker, named a, b, and c that have different genotypes and posses variation in host range within citrus species. the causative agent for type a cbc is xanthomonas citri subsp. citri, while xanthomonas fuscans subsp. aurantifolii, strain b causes type b cbc and xanthomonas fuscans s ... | 2010 | 20565886 |
| novel insights into the genomic basis of citrus canker based on the genome sequences of two strains of xanthomonas fuscans subsp. aurantifolii. | citrus canker is a disease that has severe economic impact on the citrus industry worldwide. there are three types of canker, called a, b, and c. the three types have different phenotypes and affect different citrus species. the causative agent for type a is xanthomonas citri subsp. citri, whose genome sequence was made available in 2002. xanthomonas fuscans subsp. aurantifolii strain b causes canker b and xanthomonas fuscans subsp. aurantifolii strain c causes canker c. | 2010 | 20388224 |
| adhesion and fitness in the bean phyllosphere and transmission to seed of xanthomonas fuscans subsp. fuscans. | deciphering the mechanisms enabling plant-pathogenic bacteria to disperse, colonize, and survive on their hosts provides the necessary basis to set up new control methods. we evaluated the role of bacterial attachment and biofilm formation in host colonization processes for xanthomonas fuscans subsp. fuscans on its host. this bacterium is responsible for the common bacterial blight of bean (phaseolus vulgaris), a seedborne disease. the five adhesin genes (pila, fhab, xada1, xada2, and yaph) iden ... | 2009 | 19445599 |
| polyphasic characterization of xanthomonads pathogenic to members of the anacardiaceae and their relatedness to species of xanthomonas. | we have used amplified fragment length polymorphism (aflp), multilocus sequence analysis (mlsa) and dna-dna hybridization for genotypic classification of xanthomonas pathovars associated with the plant family anacardiaceae. aflp and mlsa results showed congruent phylogenetic relationships of the pathovar mangiferaeindicae (responsible for mango bacterial canker) with strains of xanthomonas axonopodis subgroup 9.5. this subgroup includes x. axonopodis pv. citri (synonym xanthomonas citri). simila ... | 2009 | 19196770 |
| new genes of xanthomonas citri subsp. citri involved in pathogenesis and adaptation revealed by a transposon-based mutant library. | citrus canker is a disease caused by the phytopathogens xanthomonas citri subsp. citri, xanthomonas fuscans subsp. aurantifolli and xanthomonas alfalfae subsp. citrumelonis. the first of the three species, which causes citrus bacterial canker type a, is the most widely spread and severe, attacking all citrus species. in brazil, this species is the most important, being found in practically all areas where citrus canker has been detected. like most phytobacterioses, there is no efficient way to c ... | 2009 | 19149882 |
| reclassification of xanthomonas campestris pv. citri (ex hasse 1915) dye 1978 forms a, b/c/d, and e as x. smithii subsp. citri (ex hasse) sp. nov. nom. rev. comb. nov., x. fuscans subsp. aurantifolii (ex gabriel 1989) sp. nov. nom. rev. comb. nov., and x. alfalfae subsp. citrumelo (ex riker and jones) gabriel et al., 1989 sp. nov. nom. rev. comb. nov.; x. campestris pv malvacearum (ex smith 1901) dye 1978 as x. smithii subsp. smithii nov. comb. nov. nom. nov.; x. campestris pv. alfalfae (ex riker and jones, 1935) dye 1978 as x. alfalfae subsp. alfalfae (ex riker et al., 1935) sp. nov. nom. rev.; and "var. fuscans" of x. campestris pv. phaseoli (ex smith, 1987) dye 1978 as x. fuscans subsp. fuscans sp. nov. | bacterial canker of citrus is a serious disease of citrus worldwide. five forms of the disease have been described, cankers "a", "b", "c", "d", and "e". although considerable genetic diversity has been described among the causal agents of the five forms of citrus canker and supports multiple taxons, the causal agents currently are classified as pathovars citri ("a"), aurantifolii ("b/c/d") and citrumelo ("e") of a single species, xanthomonas campestris pv. citri (or x. axonopodis pv. citri). to ... | 2005 | 16104350 |