| functional characterization of replication and stability factors of an incompatibility group p-1 plasmid from xylella fastidiosa. | xylella fastidiosa strain riv11 harbors a 25-kbp plasmid (pxf-riv11) belonging to the incp-1 incompatibility group. replication and stability factors of pxf-riv11 were identified and used to construct plasmids able to replicate in x. fastidiosa and escherichia coli. replication in x. fastidiosa required a 1.4-kbp region from pxf-riv11 containing a replication initiation gene (trfa) and the adjacent origin of dna replication (oriv). constructs containing trfa and oriv from pveis01, a related incp ... | 2010 | 20935126 |
| phylogeny of replication initiator protein trfa reveals a highly divergent clade of incompatibility group p1 plasmids. | incompatibility group p1 (incp-1) plasmid diversity was evaluated based on replication initiator protein (trfa) phylogeny. a new and highly divergent clade was identified. replication assays indicated that trfa of recently discovered incp-1 plasmids from xylella fastidiosa and verminephrobacter eiseniae initiated plasmid replication using cognate or heterologous origins of replication. | 2011 | 21296948 |
| transmission of a bacterial consortium in eisenia fetida egg capsules. | the earthworm eisenia fetida harbours verminephrobacter eiseniae within their excretory nephridia. this symbiont is transferred from the parent into the egg capsules where the cells are acquired by the developing earthworm in a series of recruitment steps. previous studies defined v. eiseniae as the most abundant cell type in the egg capsules, leaving approximately 30% of the bacteria unidentified and of unknown origin. the study presented here used terminal restriction fragment length polymorph ... | 2010 | 21966919 |
| verminephrobacter eiseniae gen. nov., sp. nov., a nephridial symbiont of the earthworm eisenia foetida (savigny). | a gram-negative, flagellated, heterotrophic, catalase-negative, rod-shaped bacterium previously identified as an earthworm symbiont was isolated from nephridia of the earthworm eisenia foetida. comparisons of 16s rrna gene sequences indicated its relatedness to the betaproteobacterial genus acidovorax and the novel isolates shared 92-94% sequence similarity with recognized species of this genus. gene sequence phylogenies revealed that the group of earthworm symbionts formed a cohesive and indepe ... | 2008 | 18768621 |
| Verminephrobacter aporrectodeae sp. nov. subsp. tuberculatae and subsp. caliginosae, the specific nephridial symbionts of the earthworms Aporrectodea tuberculata and A. caliginosa. | Clone library-based studies have shown that almost all lumbricid earthworm species harbour host-specific symbiotic bacteria belonging to the novel genus Verminephrobacter in their nephridia (excretory organs). To date the only described representative from this genus is Verminephrobacter eiseniae, the specific symbiont of the earthworm Eisenia fetida. In this study two novel rod-shaped, non-endosporeforming, betaproteobacterial symbionts were isolated from the nephridia of two closely related ea ... | 2011 | 22041977 |
| application of universal stress proteins in probing the dynamics of potent degraders in complex terephthalate metagenome. | the culture-independent strategies to study microbial diversity and function have led to a revolution in environmental genomics, enabling fundamental questions about the distribution of microbes and their influence on bioremediation to be addressed. in this research we used the expression of universal stress proteins as a probe to determine the changes in degrading microbial population from a highly toxic terephthalate wastewater to a less toxic activated sludge bioreactor. the impact of relativ ... | 2013 | 24151583 |
| purifying selection and molecular adaptation in the genome of verminephrobacter, the heritable symbiotic bacteria of earthworms. | while genomic erosion is common among intracellular symbionts, patterns of genome evolution in heritable extracellular endosymbionts remain elusive. we study vertically transmitted extracellular endosymbionts (verminephrobacter, betaproteobacteria) that form a beneficial, species-specific, and evolutionarily old (60-130 myr) association with earthworms. we assembled a draft genome of verminephrobacter aporrectodeae and compared it with the genomes of verminephrobacter eiseniae and two nonsymbiot ... | 2012 | 22333491 |
| earthworm symbiont verminephrobacter eiseniae mediates natural transformation within host egg capsules using type iv pili. | the dense microbial communities commonly associated with plants and animals should offer many opportunities for horizontal gene transfer through described mechanisms of dna exchange including natural transformation (nt). however, studies of the significance of nt have focused primarily on pathogens. the study presented here demonstrates highly efficient dna exchange by nt in a common symbiont of earthworms. the obligate bacterial symbiont verminephrobacter eiseniae is a member of a microbial con ... | 2014 | 25400622 |
| verminephrobacter eiseniae type iv pili and flagella are required to colonize earthworm nephridia. | the bacterial symbiont verminephrobacter eiseniae colonizes nephridia, the excretory organs, of the lumbricid earthworm eisenia fetida. e. fetida transfers v. eisenia into the egg capsule albumin during capsule formation and v. eiseniae cells migrate into the earthworm nephridia during embryogenesis, where they bind and persist. in order to characterize the mechanistic basis of selective tissue colonization, methods for site-directed mutagenesis and colonization competence were developed and use ... | 2011 | 22170422 |