| inheritance of oryza sativa endornavirus in f1 and f2 hybrids between japonica and indica rice. | we have found a 14 kbp double-stranded rna (dsrna) in many cultivars of japonica rice (oryza sativa l.) but not in any cultivars of indica rice. this dsrna is an rna replicon with plasmid-like properties and is proposed to be a novel dsrna virus, oryza sativa endornavirus (osv). reciprocal crosses between the osv-carrier japonica variety (nipponbare) and the osv-free indica variety (ir 26 or kasalath) were performed to investigate whether osv can be transmitted to f1 hybrids. when ir 26 and nipp ... | 2003 | 12893964 |
| putative replication intermediates in endornavirus, a novel genus of plant dsrna viruses. | oryza sativa endornavirus (osv) belongs to a new genus (endornavirus) and family (endoviridae) with members containing large double-stranded rna (dsrna) replicons with plasmid-like properties. analysis of products obtained from in vitro reaction of the osv rna-dependent rna polymerase revealed a rapid increase of a population of the non-coding strand rna molecules with a head-to-tail composition. northern hybridization of total rna from osv-carrier cells with riboprobes specific for the coding s ... | 2004 | 15550778 |
| an endornavirus from a hypovirulent strain of the violet root rot fungus, helicobasidium mompa. | we determined the complete nucleotide (nt) sequence (16,614 nt) of a large double-stranded (ds) rna (referred to as l1 dsrna), previously identified as the hypovirulence factor from strain v670 of the violet root rot fungus, helicobasidium mompa. the positive-strand of l1 dsrna contained a long open reading frame (orf) potentially encoding a protein of 5,373 amino acids (molecular mass 603,080 da) with conserved motifs characteristic of rna-dependent rna polymerase (rdrp) and helicase. the orf i ... | 2006 | 16417937 |
| knock-down of osdcl2 in rice negatively affects maintenance of the endogenous dsrna virus, oryza sativa endornavirus. | an endogenous double-stranded rna (dsrna), which has recently been recognized as the dsrna virus oryza sativa endornavirus (osev), is found in many strains of cultivated rice (oryza sativa). small rnas derived from osev dsrna were detected, indicating that the rna silencing machinery recognizes osev dsrna. the existence of osev in knock-down (kd) lines of five genes of rna-dependent rna polymerase (osrdr1-osrdr5) or two genes of dicer-like protein (osdcl2 or osdcl3a) was examined to characterize ... | 2010 | 19933266 |
| evolution of and horizontal gene transfer in the endornavirus genus. | the transfer of genetic information between unrelated species is referred to as horizontal gene transfer. previous studies have demonstrated that both retroviral and non-retroviral sequences have been integrated into eukaryotic genomes. recently, we identified many non-retroviral sequences in plant genomes. in this study, we investigated the evolutionary origin and gene transfer of domains present in endornaviruses which are double-stranded rna viruses. using the available sequences for endornav ... | 2013 | 23667703 |
| molecular characterization of two evolutionarily distinct endornaviruses co-infecting common bean (phaseolus vulgaris). | two high-molecular-mass dsrnas of approximately 14 and 15 kbp were isolated from the common bean (phaseolus vulgaris) cultivar black turtle soup. these dsrnas did not appear to cause obvious disease symptoms, and were transmitted through seeds at nearly 100% efficiency. sequence information indicates that they are the genomes of distinct endornavirus species, for which the names phaseolus vulgaris endornavirus 1 (pvev-1) and phaseolus vulgaris endornavirus 2 (pvev-2) are proposed. the pvev-1 gen ... | 2013 | 23015743 |