| molecular characterisation of an australian isolate of bonamia exitiosa. | an australian (new south wales) isolate of bonamia was characterised at the molecular level by sequencing the 18s-its-1 region of the small subunit rrna operon obtained from flat oysters ostrea angasi shown to be infected by histological examination. sequence data alignment with homologous genes from 2 other isolates of bonamia (new zealand and france) revealed high levels of nucleotide identity with both isolates. however, the australian bonamia is shown to be more closely related to the new ze ... | 2006 | 16922003 |
| molecular detection and quantification of the protozoan bonamia ostreae in the flat oyster, ostrea edulis. | bonamia ostreae is an intracellular protozoan which is recognized as a cause of mortality in european populations of flat oysters (ostrea edulis). based on the recent characterization of actin genes of b. ostreae, specific primers were designed for real-time pcr using sybr green chemistry. specificity was demonstrated by the unique melting temperature peak observed in positive samples and by the lack of amplification in samples of oysters infected by closely related parasites, including bonamia ... | 2009 | 19596439 |
| observation of a bonamia sp. infecting the oyster ostrea stentina in tunisia, and a consideration of its phylogenetic affinities. | the small non-commercial oyster ostrea stentina co-occurs with commercially important ostrea edulis in the mediterranean sea, yet its disposition with respect to the destructive pathogens bonamia ostreae and marteilia refringens is unknown. we began an evaluation of the bonamia spp. infection status of o. stentina from hammamet, tunisia, in june 2007 using polymerase chain reaction diagnostics followed by histology and in situ hybridization. of 85 o. stentina sampled, nine were pcr-positive for ... | 2010 | 20036670 |
| observations raise the question if the pacific oyster, crassostrea gigas, can act as either a carrier or a reservoir for bonamia ostreae or bonamia exitiosa. | this study investigated the ability of the pacific oyster, crassostrea gigas, to act as a carrier or reservoir of the protistan bonamia ostreae. studies were carried out independently in ireland and in spain. naïve c. gigas were exposed to b. ostreae both in the field and in the laboratory via natural exposure or experimental injection. naïve flat oysters, ostrea edulis, were placed in tanks with previously exposed c. gigas. oysters were screened for b. ostreae by examination of ventricular hear ... | 2010 | 20388237 |
| gross signs and histopathology of ostrea puelchana infected by a bonamia exitiosa-like parasite (haplosporidia). | haplosporidian microcells belonging to the genus bonamia parasitise various species of oysters around the world. in argentina, bonamia sp. was the causative agent of mass mortality among ostrea puelchana cultured in san antonio bay (san matías gulf), and it was detected in natural beds inside san matías gulf. in order to describe the gross and histopathological signs caused by bonamia sp. in o. puelchana, cultured and wild oysters were sampled and analysed by traditional techniques including hea ... | 2010 | 20481090 |
| identification and expression of immune genes in the flat oyster ostrea edulis in response to bonamiosis. | the european flat ostrea edulis is highly susceptible to infection by the protozoan bonamia ostreae and bonamia exitiosa, intracellular parasites able to survive and proliferate within the oyster haemocytes. the parasite, once phagocytosed by the haemocyte, the main cellular effector of the immune system, appears to have some counter mechanism that turns off the haemocyte's metabolic destructive capacity, so that the parasite survives within the cell. to further understand the molecular basis of ... | 2012 | 22085815 |
| diseases of oysters crassostrea ariakensis and c. virginica reared in ambient waters from the choptank river, maryland and the indian river lagoon, florida. | to assess potential benefits and liabilities from a proposed introduction of asian suminoe oysters, susceptibilities of exotic crassostrea ariakensis and native c. virginica oysters were compared during exposures to pathogens endemic in temperate, mesohaline waters of chesapeake bay and sub-tropical, polyhaline atlantic waters of southern florida, usa. cohorts of diploid, sibling oysters of both species were periodically tested for diseases while reared in mesocosms receiving ambient waters from ... | 2012 | 23324414 |
| whole-genome amplification: a useful approach to characterize new genes in unculturable protozoan parasites such as bonamia exitiosa. | bonamia exitiosa is an intracellular parasite (haplosporidia) that has been associated with mass mortalities in oyster populations in the southern hemisphere. this parasite was recently detected in the northern hemisphere including europe. some representatives of the bonamia genus have not been well categorized yet due to the lack of genomic information. in the present work, we have applied whole-genome amplification (wga) technique in order to characterize the actin gene in the unculturable pro ... | 2015 | 26282916 |
| detection of bonamia exitiosa in the european flat oyster ostrea edulis in southern portugal. | | 2016 | 26096446 |
| analysis of large-scale spatio-temporal trends of ostrea puelchana beds in northern patagonian gulfs, argentina. | wild oyster populations have declined severely worldwide, however fluctuations of south atlantic populations are poorly documented. we explored the changes in the abundance of ostrea puelchana population of northern patagonia, argentina, by linking data from paleontological, archaeological and informal sources, with time series data from fishing, ecological and studies of oyster pathology. the present work is the first study which includes a south atlantic time series concerning oyster beds. the ... | 2014 | 25085814 |
| bonamia exitiosa transmission among, and incidence in, asian oyster crassostrea ariakensis under warm euhaline conditions. | previously reported in australia, new zealand, and more recently in europe, the protistan parasite bonamia exitiosa was also reported in the mid-atlantic region of the usa after causing serious mortalities there in the asian oyster crassostrea ariakensis. at the time, this oyster was being considered for introduction, and the potential consequences of introducing this species were being assessed using field and laboratory studies. b. exitiosa emerged as the most serious disease threat for this o ... | 2014 | 25060506 |
| bonamiosis status in natural ostrea puelchana beds in san matías gulf (patagonia, argentina), 14 years after an epizootic. | between 1995 and 1996, bonamia exitiosa caused an epizootic in san matías gulf, argentina, that spread from a commercial culture site of ostrea puelchana to natural beds located at the northeastern coast of the gulf. a mortality rate of 95% was registered in cultured oysters, and oysters from natural beds were also affected. the aims of this study were to assess the parasite prevalence in oyster beds and the demographic structure 14 yr after the epizootic. two different oyster beds were studied ... | 2014 | 25060505 |
| species-specific oligonucleotide probe for detection of bonamia exitiosa (haplosporidia) using in situ hybridisation assay. | bonamiosis is a disease affecting various oyster species and causing oyster mass mortalities worldwide. the protozoans bonamia exitiosa and b. ostreae (haplosporidia) are included in the list of notifiable diseases of the world organisation for animal health as the causative agents of this disease. although the geographic range of both species was considered different for years, both species are now known to co-occur in some european areas affecting the same host, ostrea edulis, which strengthen ... | 2014 | 25060500 |
| the haplosporidian bonamia exitiosa is present in australia, but the identity of the parasite described as bonamia (formerly mikrocytos) roughleyi is uncertain. | protistan oyster parasites in the genus bonamia have been observed in recent years infecting new hosts on five continents, with most of these parasites genetically similar to austral species bonamia exitiosa and bonamia roughleyi. identification of the newly observed parasites as one or another of these described species has been complicated by the fact that b. exitiosa and b. roughleyi are phylogenetically indistinguishable at the small-subunit ribosomal dna (ssu rdna) level, with samples of b. ... | 2014 | 24211185 |
| detection of bonamia exitiosa (haplosporidia) in european flat oysters ostrea edulis cultivated in mainland britain. | following a reported mortality event of european flat oysters ostrea edulis in southwestern england in december 2010, a sample of 30 oysters was examined using histology and molecular techniques. histological examination of the oysters revealed the presence of microcell stages in the haemocytes and connective tissues of 3 out of the 30 animals examined. one animal showing marked haemocyte infiltration of the connective tissues was considered to be infected with bonamia ostreae based on the prese ... | 2013 | 24113250 |
| bonamia exitiosa (haplosporidia) observed infecting the european flat oyster ostrea edulis cultured on the spanish mediterranean coast. | bonamia exitiosa and bonamia ostreae are parasites that reproduce within the haemocytes of several oyster species. in europe, the host species is the flat oyster ostrea edulis. the parasite b. ostreae has been responsible for mortalities since the late 1970s throughout the european atlantic coast. b. exitiosa was first detected, in 2007, on this continent in flat oysters cultured in galicia (nw spain). since then, the parasite has also been detected in france, italy and the united kingdom. the b ... | 2012 | 22465568 |