| methylotenera mobilis gen. nov., sp. nov., an obligately methylamine-utilizing bacterium within the family methylophilaceae. | a novel obligate methylamine utilizer (strain jlw8(t)), isolated from lake washington sediment, was characterized taxonomically. the isolate was an aerobic, gram-negative bacterium. cells were rod-shaped and motile by means of a single flagellum. reproduction was by binary fission and no resting bodies were formed. growth was observed within a ph range of 5-8.5, with optimum growth at ph 7.5. it utilized methylamine as a single source of energy, carbon and nitrogen. methylamine was oxidized via ... | 2006 | 17158982 |
| high-resolution metagenomics targets specific functional types in complex microbial communities. | most microbes in the biosphere remain unculturable. whole genome shotgun (wgs) sequencing of environmental dna (metagenomics) can be used to study the genetic and metabolic properties of natural microbial communities. however, in communities of high complexity, metagenomics fails to link specific microbes to specific ecological functions. to overcome this limitation, we developed a method to target microbial subpopulations by labeling dna through stable isotope probing (sip), followed by wgs seq ... | 2008 | 18711340 |
| insights into the physiology of methylotenera mobilis as revealed by metagenome-based shotgun proteomic analysis. | while the shotgun proteomics approach is gaining momentum in understanding microbial physiology, it remains limited by the paucity of high-quality genomic data, especially when it comes to poorly characterized newly identified phyla. at the same time, large-scale metagenomic sequencing projects produce datasets representing genomes of a variety of environmental microbes, although with lower sequence coverage and sequence quality. in this work we tested the utility of a metagenomic dataset enrich ... | 2009 | 19332812 |
| life without light: microbial diversity and evidence of sulfur- and ammonium-based chemolithotrophy in movile cave. | microbial diversity in movile cave (romania) was studied using bacterial and archaeal 16s rrna gene sequence and functional gene analyses, including ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco), soxb (sulfate thioesterase/thiohydrolase) and amoa (ammonia monooxygenase). sulfur oxidizers from both gammaproteobacteria and betaproteobacteria were detected in 16s rrna, soxb and rubisco gene libraries. dna-based stable-isotope probing analyses using 13c-bicarbonate showed that thiobacill ... | 2009 | 19474813 |
| functioning in situ: gene expression in methylotenera mobilis in its native environment as assessed through transcriptomics. | methylotrophs, organisms able to gain energy and carbon from compounds containing no carbon-carbon bonds, such as methane, methanol and methylated amines, are widespread in nature. however, knowledge of their nutrient preference and their metabolism is mostly based on experiments with cultures grown in defined laboratory conditions. here, we use transcriptomics to explore the activity of one methylotroph, methyotenera mobilis in its natural environment, lake sediment from which it has been previ ... | 2010 | 19924155 |
| karst pools in subsurface environments: collectors of microbial diversity or temporary residence between habitat types. | we studied bacterial diversity and community composition in three shallow pools of a swiss karst cave system with contrasting hydrological and hydrochemical properties. the microbial assemblages in the pools were remarkably different, and only one operational taxonomic unit of 16s rrna genes (otu, 97% similarity) was shared between the three of them (total otu number in all pools: 150). unexpectedly high microbial phylotype richness was found even in the two pools without groundwater contact and ... | 2010 | 20132276 |
| novel methylotrophic isolates from lake washington sediment and description of a new species in the genus methylotenera, methylotenera versatilis sp. nov. | phylogenetic positions as well as genotypic and phenotypic characteristics of three new methylotrophic isolates from the sediment of lake washington, seattle, usa, strains 301t, 30s and sip3-4 are described. these are restricted facultative methylotrophs capable of growth on single carbon compounds (methylamine and methanol) in addition to a limited range of multicarbon compounds. all strains use the n-methylglutamate pathway for methylamine oxidation. while strain sip3-4 possesses the canonical ... | 2011 | 21335496 |
| genomes of three methylotrophs from a single niche uncover genetic and metabolic divergence of methylophilaceae. | the genomes of three representatives of the family methylophilaceae, methylotenera mobilis jlw8, methylotenera versatilis 301 and methylovorus glucosetrophus sip3-4, all isolated from a single study site, lake washington in seattle, were completely sequenced. these were compared to each other and to the previously published genomes of methylobacillus flagellatus kt and an unclassified methylophilales strain htcc2181. comparative analysis revealed that the core genome of methylophilaceae may be a ... | 2011 | 21622745 |
| methylotrophy in a lake: from metagenomics to single organism physiology. | this review provides a brief summary of on-going studies in lake washington (seattle, usa) directed at understanding of the content and activities of microbial communities involved in methylotrophy. one of the findings from culture-independent approaches, including functional metagenomics, is the prominent presence of methylotenera species in the site and their inferred activity in c1 metabolism, highlighting the local environmental importance of this group. comparative analyses of individual ge ... | 2011 | 21622781 |
| an integrated proteomics/transcriptomics approach points to oxygen as the main electron sink for methanol metabolism in methylotenera mobilis. | methylotenera species, unlike their close relatives in the genera of methylophilus, methylobacillus and methylovorus neither exhibit the activity of methanol dehydrogenase, nor possess mxafi genes encoding this enzyme, yet they are able to grow on methanol. in this work, we integrated two genome-wide -omics approaches, shotgun proteomics and shotgun transcriptome sequencing (rna-seq) of methylotenera mobilis jlw8 to identify genes and enzymes potentially involved in methanol oxidation, with spec ... | 2011 | 21764938 |