| exopolysaccharide production by lactose-hydrolyzing bacteria isolated from traditionally fermented milk. | with increasing consumer demands for safer, healthier and more natural products, bacterially produced exopolysaccharides (epss) are becoming a viable option as an additive in designer-type foods. fresh milk samples from cattle and sheep were collected from informal settlements in south africa. after a three day incubation period at 25 degrees c, 550 bacterial strains were isolated and evaluated for eps production from lactose as sole carbon source. strains producing eps on lactose were identifie ... | 2009 | 19282044 |
| modulation of bax/bcl-2 and caspases by probiotics during acetaminophen induced apoptosis in primary hepatocytes. | oxidative stress is an important factor in drug induced hepatotoxicity and antioxidants from natural sources have potential to ameliorate it. the present study was aimed to investigate cyto-protective potential of probiotic enterococcus lactis iitrhr1 (el(sn)) and lactobacillus acidophilus mtcc447 (la(sn)) lysate against acetaminophen (apap) induced hepatotoxicity. cultured rat hepatocytes pretreated with el(sn)/la(sn) showed higher cell viability under apap stress. pre-treatment with el(sn,) re ... | 2010 | 21130831 |
| enterococcus lactis sp. nov. from italian raw milk cheeses. | the taxonomic positions of ten atypical enterococcus strains isolated from raw milk italian cheeses were investigated in a polyphasic study. 16s rrna, phenylalanyl-trna synthase alpha subunit (phes) and rna polymerase alpha subunit (rpoa) gene sequence analysis, intergenic transcribed spacer analysis and randomly amplified polymorphic dna (rapd) pcr, and an examination of phenotypic properties revealed the novel isolates to be a novel enterococcal species. on the basis of the 16s rrna analysis, ... | 2011 | 22003038 |
| isolation and characterisation of an enterocin p-producing enterococcus lactis strain from a fresh shrimp (penaeus vannamei). | screening for lactic acid bacteria (lab) from fresh shrimp samples (penaeus vannamei) collected from retail seafood markets in the tunisian's coast, resulted in the isolation of an enterococcus strain termed q1. this strain was selected for its antagonistic activity against pathogenic bacteria such as listeria monocytogenes, pseudomonas aeruginosa, lactococcus garvieae and against fungi (aspergillus niger and fusarium equiseti). the q1 strain was characterised using standard morphological and bi ... | 2017 | 28265787 |
| biotechnological and safety characterization of enterococcus lactis, a recently described species of dairy origin. | the biotechnological and safety properties of a recently described enterococcal species, enterococcus lactis, were investigated. with regard to the technological properties, in milk all the strains tested had weak acidifying and proteolytic activities, generally medium reduction activity over 24 h (-102 mv < eh < -2 mv) and low lipolytic activity on tributyrin agar. the isolates were tested for resistance against 14 antibiotics and none of the studied strains were classified as resistant to clin ... | 2013 | 22961639 |
| animal rennets as sources of dairy lactic acid bacteria. | the microbial composition of artisan and industrial animal rennet pastes was studied by using both culture-dependent and -independent approaches. pyrosequencing targeting the 16s rrna gene allowed to identify 361 operational taxonomic units (otus) to the genus/species level. among lactic acid bacteria (lab), streptococcus thermophilus and some lactobacilli, mainly lactobacillus crispatus and lactobacillus reuteri, were the most abundant species, with differences among the samples. twelve groups ... | 2014 | 24441167 |
| presence of erythromycin and tetracycline resistance genes in lactic acid bacteria from fermented foods of indian origin. | lactic acid bacteria (lab) resistant to erythromycin were isolated from different food samples on selective media. the isolates were identified as enterococcus durans, enterococcus faecium, enterococcus lactis, enterococcus casseliflavus, lactobacillus salivarius, lactobacillus reuteri, lactobacillus plantarum, lactobacillus fermentum, pediococcus pentosaceus and leuconostoc mesenteroides. of the total 60 isolates, 88 % harbored the ermb gene. the efflux gene msra was identified in e. faecium, e ... | 2012 | 22644346 |
| microbiological characterization using combined culture dependent and independent approaches of casizolu pasta filata cheese. | casizolu is a traditional sardinian (italy) pasta filata cheese made with cow raw milk belonging to sardo-modicana and/or bruno-sarda breeds added with natural whey starter. this work aims to describe the traditional technology of this product and to evaluate the microbial groups/species involved in the first month of ripening. | 2016 | 26551888 |
| selection of amine-oxidizing dairy lactic acid bacteria and identification of the enzyme and gene involved in the decrease of biogenic amines. | accumulation of biogenic amines (bas) in cheese and other foods is a matter of public health concern. the aim of this study was to identify the enzyme activities responsible for ba degradation in lactic acid bacteria which were previously isolated from traditional sicilian and apulian cheeses. the selected strains would control the concentration of bas during cheese manufacture. first, 431 isolates not showing genes encoding the decarboxylases responsible for ba formation were selected using pcr ... | 2016 | 27637883 |
| the prophylactic effect of probiotic enterococcus lactis iw5 against different human cancer cells. | enterococcus lactis iw5 was obtained from human gut and the potential probiotic characteristics of this organism were then evaluated. results showed that this strain was highly resistant to low ph and high bile salt and adhered strongly to caco-2 human epithelial colorectal cell lines. the supernatant of e. lactis iw5 strongly inhibited the growth of several pathogenic bacteria and decreased the viability of different cancer cells, such as hela, mcf-7, ags, ht-29, and caco-2. conversely, e. lact ... | 2015 | 26635778 |
| probiotic enterococcus lactis iitrhr1 protects against acetaminophen-induced hepatotoxicity. | objective: acetaminophen (apap), an antipyretic/analgesic drug, is reported to cause toxicity on overdose. dietary supplements are currently being explored to decrease toxicity. in the present study, the protective effect of probiotic enterococcus lactis iitrhr1 was evaluated at different doses (10(7), 10(8), and 10(9) colony-forming units) against apap-induced liver damage. methods: male wistar rats were administered apap (1 g/kg of body weight orally) for 14 d, and hepatotoxicity was assessed ... | 2011 | 21778036 |