| group-specific antigen shared by the members of the reticuloendotheliosis virus complex. | the polypeptides of reticuloendotheliosis virus (rev) were separated by gel filtration in the presence of guanidine hydrochloride. the eight peaks obtained by gel filtration were then analyzed by polyacrylamide gel electrophoresis and four appeared to contain single polypeptides. the material identified as p29 was used to prepare antiserum. this protein constitutes the major internal non-glycosylated polypeptide in the virion. double immunodiffusion indicated that the antiserum was specific for ... | 1976 | 56462 |
| polypeptide and rna composition of the reticuloendotheliosis viruses. | the rna and polypeptide composition of chick syncytial virus (csv) and duck infectious anemia virus (diav) was investigated and compared to that of reticuloendotheliosis virus (rev) strain t, the prototype of the newly recognized rev group of viruses. csv and diav contain genomic rna species which cosediment with those of rev in sucrose gradients. five or six polypeptides, two of which are glycoproteins, were consistently found in csv and diav preparations. the major nonglycosylated polypeptides ... | 1975 | 172472 |
| complement-fixation test for reticuloendotheliosis viruses: limits of sensitivity in infected avian cells. | a specific micro-complement-fixation procedure for assay of avian reticuloendotheliosis viruses (rev), designated by use as the cofar test, was compared with an assay based on immunofluorescent (if) antibody staining of infected chick embryo fibroblasts. endpoint titrations in which rev strain t, chick syncytial virus, and spleen necrosis virus were used indicated that cultures infected with limiting dilutions of each strain were positive by both procedures within 6 days. depending on cell densi ... | 1977 | 204281 |
| differentiation of pathogenic and non-pathogenic serotype 1 marek's disease viruses (mdvs) by the polymerase chain reaction amplification of the tandem direct repeats within the mdv genome. | there are no simple, direct methods to reliably distinguish oncogenic serotype 1 marek's disease viruses (mdvs) from their attenuated variants. the present study was an attempt to apply polymerase chain reaction (pcr) to develop a rapid and sensitive assay for the presence of the mdv genome. pcr oligos were chosen to flank the 132-base-pair tandem direct repeats in the serotype 1 mdv genome. the pcr reaction was specific for serotype 1 mdvs, amplifying fragments corresponding to one to three cop ... | 1992 | 1329706 |
| monoclonal antibodies against avian reticuloendotheliosis virus: identification of strain-specific and strain-common epitopes. | we report the generation and partial characterization of a panel of 11 monoclonal antibodies (mca) made against the nondefective strain t reticuloendotheliosis virus (rev). in an enzyme-linked immunosorbent assay (elisa), nine mca cross-reacted with both homologous strain t and heterologous strain chick syncytial virus (cs), whereas two mca were strain specific and reacted with strain t but not with cs. competitive antibody inhibitory elisa tests demonstrated that the nine mca recognized at leas ... | 1986 | 2422277 |
| a nonimmunosuppressive helper virus allows high efficiency induction of b cell lymphomas by reticuloendotheliosis virus strain t. | we have documented the effect of two nondefective helper viruses, reticuloendotheliosis virus a (rev-a) and chick syncytial virus (csv) infection on bursal tissue. rev-a infection results in bursal atrophy, destroying both its structural and functional integrity. in contrast, the bursae in csv-infected chicks, while reduced slightly in size, appear both structurally and functionally normal. rev-a-induced bursal atrophy is not a result of viral replication in the b-lymphocyte as (a) both viruses ... | 1988 | 2826639 |
| monoclonal-antibody-mediated enzyme-linked immunosorbent assay for detection of reticuloendotheliosis viruses. | an enzyme-linked immunosorbent assay (elisa) is described for the detection of reticuloendotheliosis viruses (revs). the assay uses a mixture of monoclonal antibodies (mcas) prepared against a 62-kilodalton rev envelope glycoprotein (gp62) to capture antigen, rabbit anti-rev serum as detection antibody, and peroxidase-conjugated anti-rabbit igg as indicator antibody. the mcas were reactive with rev strain t, chick syncytial virus, and duck infectious anemia virus but unreactive against marek's d ... | 1988 | 2838016 |
| an igm-producing b lymphoblastoid cell line established from lymphomas induced by a non-defective reticuloendotheliosis virus. | chick syncytial virus (csv), a strain of avian reticuloendotheliosis virus (rev) causes lymphoid tumours in chickens after a prolonged incubation period. a number of csv-induced tumours were examined for cell surface antigen and were found to be of the b cell type and to produce immunoglobulin. attempts were made to grow in vitro cell lines from csv-induced tumours and a lymphoblastoid cell line was established from a liver tumour of a chicken that was inoculated with csv via the yolk sac in emb ... | 1982 | 6278061 |
| effect of endogenous leukosis virus genes on response to infection with avian leukosis and reticuloendotheliosis viruses. | we examined the effect of the presence or absence of endogenous viral gene (ev) 3, which controls expression of group-specific viral and envelope antigens (gs+chf+ phenotype), and ev2, which controls the production of a complete subgroup e virus (v-e+ phenotype), on the response of chickens to rav-1, an exogenous avian leukosis virus (alv) with an antigenic relationship to endogenous virus. after inoculation at one day of age, the chickens lacking either ev gene expression had a lower frequency ... | 1982 | 6285878 |
| phylogenetic analyses indicate little variation among reticuloendotheliosis viruses infecting avian species, including the endangered attwater's prairie chicken. | reticuloendotheliosis virus infection, which typically causes systemic lymphomas and high mortality in the endangered attwater's prairie chicken, has been described as a major obstacle in repopulation efforts of captive breeding facilities in texas. although antigenic relationships among reticuloendotheliosis virus (rev) strains have been previously determined, phylogenetic relationships have not been reported. the pol and env of rev proviral dna from prairie chickens (pc-r92 and pc-2404), from ... | 2006 | 16497405 |
| detection of reticuloendotheliosis virus infection using the polymerase chain reaction. | the polymerase chain reaction (pcr) was shown to be a sensitive and useful method for detection of infection by members of the group of avian reticuloendotheliosis virus (rev). genomic dna extracted from chick embryo fibroblasts (cefs), blood and tumours of chickens experimentally infected with the spleen necrosis virus (snv) strain of rev was used as the target for chain elongation. deoxyoligonucleotide primers that encompass a portion of the unique 3', repeat and unique 5' region of the long t ... | 1993 | 18671039 |
| stimulation of local solid tumour development of the nonproducer marek's disease tumour transplant jmv by virus-induced immunosuppression. | chickens could be protected against lethal lymphoblastic leukaemia due to the nonproducer jmv marek's disease (md) tumour transplant by infection with the herpesvirus of turkeys (hvt) or various strains of md virus. however, solid jmv tumours developed in md virus-infected birds at the site of intramuscular or subcutaneous transplantation, but tumours never developed at the site of md virus inoculation. the incidence and extent of local tumour growth, the development of metastases and the inhibi ... | 1980 | 18770243 |
| immunological effects of reticuloendotheliosis virus as potential contaminant of marek's disease vaccines. | certain properties of four strains of reticuloendotheliosis virus (rev), namely rev-f, rev-s, rev-c and chick syncytial virus (csv) were studied in vivo and in vitro. in chicken embryo fibroblast (cef) cultures these viruses caused a chronic infection which could be detected by indirect immunofluorescence (fa) tests. precipitating antigens were present in infected cultured cells as well as in culture supernatants. antibodies in sera of rev-inoculated chickens were demonstrated by fa tests and ag ... | 1977 | 18770347 |
| characterization of reticuloendotheliosis virus isolates obtained from broiler breeders, turkeys, and prairie chickens located in various geographical regions in the united states. | nine reticuloendotheliosis virus (rev) isolates obtained from broiler breeders, turkeys, and prairie chickens located in three different geographical regions in the usa, and three isolates obtained from known contaminated live-virus vaccines were characterized using polymerase chain reaction (pcr) and indirect immunofluorescence (ifa) assays. all isolates were propagated in chicken embryo fibroblasts obtained from a specific pathogen free breeder flock. pcr analysis of all 12 isolates resulted i ... | 2010 | 20954015 |
| first isolation of reticuloendotheliosis virus from mallards in china. | reticuloendotheliosis virus (rev) causes an oncogenic, immunosuppressive and runting syndrome in many avian hosts worldwide. rev infection has never been reported in mallard ducks, however. to identify rev infection in mallards, we collected 40 mallard duck samples from jilin province of china. in this study, the rev strain, dbyr1102, was first isolated from a mallard in china and identified by pcr, indirect immunofluorescence assay and electron microscopy. the gp90 gene and complete ltr of dbyr ... | 2014 | 24643331 |