| new insight into diversity in the genus xenorhabdus, including the description of ten novel species. | we investigated the diversity of a collection of 76 xenorhabdus strains, isolated from at least 27 species of steinernema nematodes and collected in 32 countries, using three complementary approaches: 16s rrna gene sequencing, molecular typing and phenotypic characterization. the 16s rrna gene sequences of the xenorhabdus strains were highly conserved (similarity coefficient >95 %), suggesting that the common ancestor of the genus probably emerged between 250 and 500 million years ago. based on ... | 2006 | 17158981 |
| a practical guide to recombineering in photorhabdus and xenorhabdus. | fluent genetic manipulation of prokaryote genomes is still limited to only a few commonly used hosts. ideally the advanced technologies available for cloning into recombinant escherichia coli should also be applicable in other prokaryotes. in particular, 'recombineering' is mediated by the lambda red operon that permits fluent and precise engineering of the e. coli genome and associated recombinant dna. the major limitation is that host-specific phage-derived recombination systems are also requi ... | 2017 | 28154939 |
| xenorhabdus stockiae kt835471-mediated feasible biosynthesis of metal nanoparticles for their antibacterial and cytotoxic activities. | in this study, extracellular metabolites of symbiotic bacteria xenorhabdus stockiae (kt835471) was employed for the synthesis of silver (xsagnps) and gold nanoparticles (xsaunps). synthesized nps were characterized using high throughput instrumentation which confirms the generation of stable, crystalline xsagnps and xsaunps with the mean size of 14 ± 6 and 14 ± 5, respectively. further, the nps exhibits an excellent bactericidal effect against six different pathogens. on the other hand, nps disp ... | 2017 | 28140656 |