isolation in cell culture of nodavirus from farmed atlantic halibut hippoglossus hippoglossus in norway.isolation in cell culture of nodavirus from atlantic halibut hippoglossus hippoglossus suffering from viral encephalopathy and retinopathy (ver) is described. the cell line ssn-1 was inoculated with tissue material from affected juveniles (60 d after first feeding). extensive cytopathic effects (cpe) developed approximately 5 d after inoculation, and were also observed after several passages in the same cell line. cells from infected cultures showed reactivity with an antiserum against sea bass ...200011206733
pathogenicity of nodavirus strains from striped jack pseudocaranx dentex and atlantic halibut hippoglossus hippoglossus, studied by waterborne challenge of yolk-sac larvae of both teleost species.the present study shows that differences in pathogenicity exist among fish nodavirus strains. in challenge trials, a japanese strain (sj93nag) was highly virulent to larvae of the striped jack pseudocaranx dentex but replication was not detected in larvae of atlantic halibut hippoglossus hippoglossus at 6 degrees c. conversely, a norwegian nodavirus strain (ah95nora) that was highly virulent to the atlantic halibut larvae did not replicate in striped jack larvae at 20 degrees c. occurrence of th ...199910686668
antigenic relationship of the picorna-like virus of larval barramundi, lates calcarifer bloch to the nodavirus of larval striped jack, pseudocaranx dentex (bloch & schneider). 19947726766
properties of a new virus belonging to nodaviridae found in larval striped jack (pseudocaranx dentex) with nervous necrosis.spherical virus particles were purified from larval striped jack (pseudocaranx dentex) with nervous necrosis. the virus consists of nonenveloped particles, about 25 nm in diameter, and contains two single-stranded, positive-sense rna molecules with molecular weights of 1.01 x 10(6)da (rna 1) and 0.49 x 10(6)da (rna 2), respectively. the rnas do not have poly(a) sequences at the 3' terminus. virus structural proteins consist of two proteins with molecular weights of 42 and 40 kda. when translated ...19921736540
bacterial vaccines for fish--an update of the current situation worldwide.during the last few years, the use of vaccines for disease prevention in aquaculture has expanded both with regard to the number of fish species and number of microbial diseases. according to the responses to a questionnaire received from 41 countries, vaccination is used in the commercial aquaculture of species like atlantic salmon (salmo salar), rainbow trout (oncorhynchus mykiss), sea bass (dicentrarchus labrax), sea bream (sparus aurata), barramundi (lates calcarifer), tilapia (tilapia spp), ...200515962470
characterization of striped jack nervous necrosis virus subgenomic rna3 and biological activities of its encoded protein b2.striped jack nervous necrosis virus (sjnnv), which infects fish, is the type species of the genus betanodavirus. this virus has a bipartite genome of positive-strand rnas, designated rnas 1 and 2. a small rna (ca. 0.4 kb) has been detected from sjnnv-infected cells, which was newly synthesized and corresponded to the 3'-terminal region of rna1. rapid amplification of cdna ends analysis showed that the 5' end of this small rna (designated rna3) initiated at nt 2730 of the corresponding rna1 seque ...200516186236
variable region of betanodavirus rna2 is sufficient to determine host specificity.betanodaviruses, the causative agents of viral nervous necrosis in marine fish, have bipartite positive-sense rna genomes. the viruses have been classified into 4 distinct types based on nucleotide sequence similarities in the variable region (the so-called t4 region) of the smaller genomic segment rna2 (1.4 kb). betanodaviruses have marked host specificity, although the primary structures of the viral rnas and encoded proteins are similar among the viruses. we have previously demonstrated, usin ...200818589996
exophiala xenobiotica infection in cultured striped jack, pseudocaranx dentex (bloch & schneider), in japan.this report describes exophiala infection in cultured striped jack, pseudocaranx dentex, in japan in 2005. one hundred out of 35,000 fish died per day and mortalities continued for 1 month. diseased fish showed swelling of the abdomen and kidney distension. numerous septate hyphae, pale brown in colour, were seen in kidney in squash preparations. histology revealed abundant fungal hyphae and conidia in gill, heart and kidney. fungal hyphae were accompanied by cell necrosis and influx of inflamma ...200919735347
homogeneity among lactococcus garvieae isolates from striped jack, pseudocaranx dentex (bloch & schneider), and its ectoparasites. 200919500211
structural characterization of carangid fish myoglobins.the primary structures of myoglobin (mb) from the following five carangid species were determined: yellowtail seriola quinqueradiata, greater amberjack seriola dumerili, yellowtail kingfish seriola lalandi, japanese horse mackerel trachurus japonicus, and silver trevally pseudocaranx dentex. the sequences were of varying composition both in the coding and in the noncoding regions, but all contained the open reading frame of 444 nucleotides encoding 147 amino acids. amino acid sequence identities ...201222361749
speciation of gram-positive bacteria in fresh and ambient-stored sub-tropical marine fish.this study identified gram-positive bacteria in three sub-tropical marine fish species; pseudocaranx dentex (silver trevally), pagrus auratus (snapper) and mugil cephalus (sea mullet). it further elucidated the role played by fish habitat, fish body part and ambient storage on the composition of the gram-positive bacteria. a total of 266 isolates of gram-positive bacteria were identified by conventional biochemical methods, vitek, pcr using genus- and species-specific primers and/or 16s rrna gen ...201020110133
genotypic characteristics of a mycobacterium sp. isolated from yellowtail seriola quinqueradiata and striped jack pseudocaranx dentex in japan, a mycobacterium marinum-like mycobacterium was isolated from the yellowtail, seriola quinqueradiata. the species was identified as m. marinum by a commercial mycobacterial dna-dna hybridization kit. nevertheless, pcr restriction analysis of the dna of its rna polymerase β-subunit gene definitively showed that this mycobacterium sp. was m. ulcerans. pcr analysis revealed the genotypic characteristics of m. ulcerans in the mycobacterium sp., only the mup053 gene sequence being absent, as ...201323043488
monostephanostomum nolani sp. n. and m. krusei reimer, 1983 (digenea: acanthocolpidae) from carangid fishes from coral reef waters off australia.monostephanostonum nolani sp. n. is described from carangoides plagiotaenia bleeker, off lizard island, great barrier reef, queensland, australia. it differs from all other species in the genus except m. manteri kruse, 1979 in that the vitellarium reaches into the forebody. it differs from m. manteri in the ventral hiatus in the circum-oral spine row, the extent of the vitellarium in the forebody, where it is not confluent, its elongate pharynx and its smaller eggs. monostephanostoinum krusei re ...200717441433
molecular characterization of anisakid nematode larvae from 13 species of fish from western australia.this study characterized anisakid nematodes in estuarine and near-shore species of fish in southern western australia. a total of 108 fish representing 13 species were examined for anisakid larvae. for the molecular characterization of anisakid larvae (n=218), we used pcr-coupled mutation scanning-sequencing-phylogenetic analyses of sequence variation in the internal transcribed spacers of nuclear ribosomal dna. with the exception of sillaginoides punctatus and sillago schomburgkii, all the fish ...201223353682
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